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The map label for this gene is thiE [H]

Identifier: 238924878

GI number: 238924878

Start: 2402445

End: 2403095

Strand: Reverse

Name: thiE [H]

Synonym: EUBREC_2529

Alternate gene names: 238924878

Gene position: 2403095-2402445 (Counterclockwise)

Preceding gene: 238924879

Following gene: 238924877

Centisome position: 69.66

GC content: 49.16

Gene sequence:

>651_bases
ATGAACGAAACTGATTTAAAGCTCTACGCTATAACAGACAGGCAGTGGCTGCACGGAGCTAGGCTTTCTGAGCATGTGAA
GCTTGCGATAGAGGGCGGTGCCACTATGATTCAGATCCGTGACAAGGATATTCTGAGTACGGATTCTGATGCAGGGCTTA
AGGATGAGTATAGTGAGGCTCTTGAGATAAAGAGAATCTGTCATGAGCACAAGGTGCCGCTCATCATCAATGATAACGTG
CAGTTTGCAATTGATATAGCTGCCGATGGAGTACATCTGGGACAGGATGACATGAATCCGGCGGAGGCCAGGAAGCTTCT
TGGAACTGACAAGATAATAGGAGTCACGGCAAAGACCGTGGAGCAGGCAAAGAGGGCAGAAGCTGATGGCGCTGACTATC
TGGGAAGCGGAGCTGTGTTTGGCTCTACCACAAAGCTCAATGCAAAGCCTATGACAAAGGAGCTTCTGCGTGAAATCACG
GCGGCTGTCGATATACCGGTTGTTGCAATCGGAGGCATAAATGCAGACAATGCGGTCACGCTAAAGGGCACAGGGATTGC
AGGAATCGCAGTGGTAGGAGCGATATTTGCAAGCGCAGATATAAAGGCTGCAGCCAAGGAACTCGCAGGAATATGCGACA
AAATTCTGTAA

Upstream 100 bases:

>100_bases
ATTATGAACTGATGTGAATATCGCTGTATGACAGACAATTGGTATGCAGGTCATGAGTCTTATATTTGGAAAACAACAGG
ATAAGAGGTGAAAGGTATAT

Downstream 100 bases:

>100_bases
TCACAGTATGACATACTGCTATAACTACAGTAATTAAACAATATTTTGAAGATACTGAACAGTTACGAGAAATCACAACA
TTCAGTAGAAAGGAGCGTAA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MNETDLKLYAITDRQWLHGARLSEHVKLAIEGGATMIQIRDKDILSTDSDAGLKDEYSEALEIKRICHEHKVPLIINDNV
QFAIDIAADGVHLGQDDMNPAEARKLLGTDKIIGVTAKTVEQAKRAEADGADYLGSGAVFGSTTKLNAKPMTKELLREIT
AAVDIPVVAIGGINADNAVTLKGTGIAGIAVVGAIFASADIKAAAKELAGICDKIL

Sequences:

>Translated_216_residues
MNETDLKLYAITDRQWLHGARLSEHVKLAIEGGATMIQIRDKDILSTDSDAGLKDEYSEALEIKRICHEHKVPLIINDNV
QFAIDIAADGVHLGQDDMNPAEARKLLGTDKIIGVTAKTVEQAKRAEADGADYLGSGAVFGSTTKLNAKPMTKELLREIT
AAVDIPVVAIGGINADNAVTLKGTGIAGIAVVGAIFASADIKAAAKELAGICDKIL
>Mature_216_residues
MNETDLKLYAITDRQWLHGARLSEHVKLAIEGGATMIQIRDKDILSTDSDAGLKDEYSEALEIKRICHEHKVPLIINDNV
QFAIDIAADGVHLGQDDMNPAEARKLLGTDKIIGVTAKTVEQAKRAEADGADYLGSGAVFGSTTKLNAKPMTKELLREIT
AAVDIPVVAIGGINADNAVTLKGTGIAGIAVVGAIFASADIKAAAKELAGICDKIL

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=215, Percent_Identity=31.6279069767442, Blast_Score=75, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6325042, Length=232, Percent_Identity=37.9310344827586, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 22841; Mature: 22841

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNETDLKLYAITDRQWLHGARLSEHVKLAIEGGATMIQIRDKDILSTDSDAGLKDEYSEA
CCCCCEEEEEECCCHHHCCCCCCCEEEEEEECCEEEEEEECCCCCCCCCCCCCHHHHHHH
LEIKRICHEHKVPLIINDNVQFAIDIAADGVHLGQDDMNPAEARKLLGTDKIIGVTAKTV
HHHHHHHHHCCCCEEEECCCEEEEEEECCCEECCCCCCCHHHHHHHHCCCCEEEHHHHHH
EQAKRAEADGADYLGSGAVFGSTTKLNAKPMTKELLREITAAVDIPVVAIGGINADNAVT
HHHHHCCCCCCCCCCCCCEECCCEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEE
LKGTGIAGIAVVGAIFASADIKAAAKELAGICDKIL
EECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNETDLKLYAITDRQWLHGARLSEHVKLAIEGGATMIQIRDKDILSTDSDAGLKDEYSEA
CCCCCEEEEEECCCHHHCCCCCCCEEEEEEECCEEEEEEECCCCCCCCCCCCCHHHHHHH
LEIKRICHEHKVPLIINDNVQFAIDIAADGVHLGQDDMNPAEARKLLGTDKIIGVTAKTV
HHHHHHHHHCCCCEEEECCCEEEEEEECCCEECCCCCCCHHHHHHHHCCCCEEEHHHHHH
EQAKRAEADGADYLGSGAVFGSTTKLNAKPMTKELLREITAAVDIPVVAIGGINADNAVT
HHHHHCCCCCCCCCCCCCEECCCEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEE
LKGTGIAGIAVVGAIFASADIKAAAKELAGICDKIL
EECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA