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The map label for this gene is thiM [H]

Identifier: 238924879

GI number: 238924879

Start: 2403179

End: 2404054

Strand: Reverse

Name: thiM [H]

Synonym: EUBREC_2530

Alternate gene names: 238924879

Gene position: 2404054-2403179 (Counterclockwise)

Preceding gene: 238924881

Following gene: 238924878

Centisome position: 69.69

GC content: 48.97

Gene sequence:

>876_bases
ATGAATAGTTGTAAAGAGATACAGATGAATAATTTTATAGAAAAGCTTAAGTCACGCAGTCCTTTGATTCACAACATCAC
AAATATGGTTACTATCAATGATGTGGCCAATATTGAGCTGGCATGTGGGGCCAGGCCTATTATGGCTATGGCACCGCAGG
AGATGGATGAGGTCACGGGTATCTGCGATGGGCTGAATCTGAATATAGGCACTCCCTCTGAGGAGCGCTTTGAGGCGATG
CGCATTGCTGCGAGGATGGCAGCGAAAAAGAACATTCCTATTGTGCTTGATCTGGTTGGTGTGGGCGTGAGCCGCTTCAG
GATGGATTTTGTGATGAGCCTGCTTGACGAGGTGCGTGTGGATGTGATTAAGGGCAATTTGTCTGAGGTCAAGGCTGTTA
TGGAGCATGGCAGGTGCGATGGCGGCGTGGAAGTGACCGATACGGCAGATGAGTCTGATGCAAAGGCGCTTGCGTGCAGG
GCGGCCCTGCGATATGGCAGCATCTGTGTGATTACAGGTGAGACCGATTATGTGGCAGAATTATGTGATGAAGCTGATTG
CTATGACAATAATGAAAGAAGTGTGATGTGCATCACAGGCGGCCATCCTATGATGAAGCGGGTGACAGGCACCGGATGTA
TGCTTTCAGGGCTCATTTGTGCATTTGTTGCGGCTGATTGCGATGATAAATACGGTGCAGTCACGGCAGCACTTAGCTGC
ATGAAGAGTGCAGGCGGGCTTGCTGCCTCTGATATGGCAGAGCACGGCAGAGAGACTAACAGCTGTTATTTCATTAAGCC
CGGAAATGCTGCATACCGGGACAGGCTGATTGATGCAGTATATCATATATGCGATGGTGATTATGAACTGATGTGA

Upstream 100 bases:

>100_bases
TAACCTGATTTGGATAATGCCAACGTAGGATAATATCGCACGATTTTTAGGCGGATATACTCATTGGCGGTATATCCGCT
TTTTGAATGTGTGGGTGATT

Downstream 100 bases:

>100_bases
ATATCGCTGTATGACAGACAATTGGTATGCAGGTCATGAGTCTTATATTTGGAAAACAACAGGATAAGAGGTGAAAGGTA
TATATGAACGAAACTGATTT

Product: hydroxyethylthiazole kinase ThiM/ThiK

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MNSCKEIQMNNFIEKLKSRSPLIHNITNMVTINDVANIELACGARPIMAMAPQEMDEVTGICDGLNLNIGTPSEERFEAM
RIAARMAAKKNIPIVLDLVGVGVSRFRMDFVMSLLDEVRVDVIKGNLSEVKAVMEHGRCDGGVEVTDTADESDAKALACR
AALRYGSICVITGETDYVAELCDEADCYDNNERSVMCITGGHPMMKRVTGTGCMLSGLICAFVAADCDDKYGAVTAALSC
MKSAGGLAASDMAEHGRETNSCYFIKPGNAAYRDRLIDAVYHICDGDYELM

Sequences:

>Translated_291_residues
MNSCKEIQMNNFIEKLKSRSPLIHNITNMVTINDVANIELACGARPIMAMAPQEMDEVTGICDGLNLNIGTPSEERFEAM
RIAARMAAKKNIPIVLDLVGVGVSRFRMDFVMSLLDEVRVDVIKGNLSEVKAVMEHGRCDGGVEVTDTADESDAKALACR
AALRYGSICVITGETDYVAELCDEADCYDNNERSVMCITGGHPMMKRVTGTGCMLSGLICAFVAADCDDKYGAVTAALSC
MKSAGGLAASDMAEHGRETNSCYFIKPGNAAYRDRLIDAVYHICDGDYELM
>Mature_291_residues
MNSCKEIQMNNFIEKLKSRSPLIHNITNMVTINDVANIELACGARPIMAMAPQEMDEVTGICDGLNLNIGTPSEERFEAM
RIAARMAAKKNIPIVLDLVGVGVSRFRMDFVMSLLDEVRVDVIKGNLSEVKAVMEHGRCDGGVEVTDTADESDAKALACR
AALRYGSICVITGETDYVAELCDEADCYDNNERSVMCITGGHPMMKRVTGTGCMLSGLICAFVAADCDDKYGAVTAALSC
MKSAGGLAASDMAEHGRETNSCYFIKPGNAAYRDRLIDAVYHICDGDYELM

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788421, Length=239, Percent_Identity=34.7280334728033, Blast_Score=113, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6325042, Length=288, Percent_Identity=28.8194444444444, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000417 [H]

Pfam domain/function: PF02110 HK [H]

EC number: =2.7.1.50 [H]

Molecular weight: Translated: 31517; Mature: 31517

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.2 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
11.3 %Cys+Met (Translated Protein)
5.2 %Cys     (Mature Protein)
6.2 %Met     (Mature Protein)
11.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSCKEIQMNNFIEKLKSRSPLIHNITNMVTINDVANIELACGARPIMAMAPQEMDEVTG
CCCCHHHHHHHHHHHHHHCCCHHHHHHCEEEECCCCCEEEECCCCCHHHCCHHHHHHHHH
ICDGLNLNIGTPSEERFEAMRIAARMAAKKNIPIVLDLVGVGVSRFRMDFVMSLLDEVRV
HHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHH
DVIKGNLSEVKAVMEHGRCDGGVEVTDTADESDAKALACRAALRYGSICVITGETDYVAE
HHHCCCHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCEEEEECCHHHHHH
LCDEADCYDNNERSVMCITGGHPMMKRVTGTGCMLSGLICAFVAADCDDKYGAVTAALSC
HHCCCCCCCCCCCEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
MKSAGGLAASDMAEHGRETNSCYFIKPGNAAYRDRLIDAVYHICDGDYELM
HHHCCCCCHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEECC
>Mature Secondary Structure
MNSCKEIQMNNFIEKLKSRSPLIHNITNMVTINDVANIELACGARPIMAMAPQEMDEVTG
CCCCHHHHHHHHHHHHHHCCCHHHHHHCEEEECCCCCEEEECCCCCHHHCCHHHHHHHHH
ICDGLNLNIGTPSEERFEAMRIAARMAAKKNIPIVLDLVGVGVSRFRMDFVMSLLDEVRV
HHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHH
DVIKGNLSEVKAVMEHGRCDGGVEVTDTADESDAKALACRAALRYGSICVITGETDYVAE
HHHCCCHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCEEEEECCHHHHHH
LCDEADCYDNNERSVMCITGGHPMMKRVTGTGCMLSGLICAFVAADCDDKYGAVTAALSC
HHCCCCCCCCCCCEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
MKSAGGLAASDMAEHGRETNSCYFIKPGNAAYRDRLIDAVYHICDGDYELM
HHHCCCCCHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]