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The map label for this gene is sbp [H]

Identifier: 238924825

GI number: 238924825

Start: 2344274

End: 2345293

Strand: Reverse

Name: sbp [H]

Synonym: EUBREC_2476

Alternate gene names: 238924825

Gene position: 2345293-2344274 (Counterclockwise)

Preceding gene: 238924826

Following gene: 238924824

Centisome position: 67.99

GC content: 44.9

Gene sequence:

>1020_bases
ATGAGAAATATACATAAATACACGGCAGCCATGCTGTCAGCGGTGATGGTGGTGTCACTTTTTCTGACAGGCTGCTCAGG
TAAGAAGGATGATGGCACAGTCACCATAACGAATGTGTCATATGATCCGACACGAGAGTTTTACGAAAAATACAACAAAA
TATTCGAGTCTTACTATGAGAGCGAGCATGGCAAAAGGATAAATGTGATTCAGTCACATGGCGGCTCGGGAGCGCAGGCT
CGTTCGGTTGTGGAGGGCTGTAATGCAGATGTTGTCACACTGGCACTCGAGCATGACATAGACCTGATACAAAATACCGG
CTTAATTGAAAAGGGCTGGATTGATGAATTTCCAAAAAGCTCTGCACCGTACACATCAACCATCGTTTTTCTTGTAAGAA
AGGGCAATCCAAAGCATATAAGCGACTGGGATGACCTGGTGAAAAAGAATGTGGATGTGATTACACCGGATCCAAAGAGC
AGTGGCGGAGCCTGCTGGAATTTCCTTGCGGCGCTAAGCTATGCGAAGACAAACTATGACAGCCAAAAGGAACAGAAAAC
GTTTATGAGAAAGCTGTATGAAAATGTCACAATTATGGACTCAGGTGCGAGAGGCTCAACAACCACATTTGTGGAAAACG
GTCAGGGAGATGTGCTTATAGCGTGGGAAAACGAGGCACTTACGACCATGGATTCATATCCGGGTAAGTATGAGATAGTG
AATCCGTCAGTCAGCATCTTAGCGCAGCCGTCTGTTGCCATTGTGGATGATAATGCAAAGAGCAATGGAACAGAAGCTGC
GAGTAAAGAGTACTTACAGTATCTCTACAGCGATGAGGCACAGAGGCTGATTGGAGAGAGTGGCTACAGACCAACCAATC
AGGACATATTAAATCAGTTTTCCGACAAGTTTGATTTGAATATCAGGCTTTGGAGCATAGATGATTACGGCGGATGGGAT
GAAGCCTACAAGACATATTTCGATGATGGAGCAATGTTCGATGAAATTTACGGCAACTGA

Upstream 100 bases:

>100_bases
CTGTCCACACAGCTTTTTACAGTAAACTAATGGTATATAGGACAACCGACGGCATAAGCTACAGTGTCTGAATCTGTAAT
ATGAAAAATGAGAAATATAC

Downstream 100 bases:

>100_bases
TAAAACAGCAAAAAAGAAAACTAAATCAAAGAACATAAAGACCAGAGTTATACCGGGCTACCATCTTTCGCTTGGGATAG
TGGTCACGATGCTTTCAGTC

Product: substrate binding protein of an ABC transporter complex

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MRNIHKYTAAMLSAVMVVSLFLTGCSGKKDDGTVTITNVSYDPTREFYEKYNKIFESYYESEHGKRINVIQSHGGSGAQA
RSVVEGCNADVVTLALEHDIDLIQNTGLIEKGWIDEFPKSSAPYTSTIVFLVRKGNPKHISDWDDLVKKNVDVITPDPKS
SGGACWNFLAALSYAKTNYDSQKEQKTFMRKLYENVTIMDSGARGSTTTFVENGQGDVLIAWENEALTTMDSYPGKYEIV
NPSVSILAQPSVAIVDDNAKSNGTEAASKEYLQYLYSDEAQRLIGESGYRPTNQDILNQFSDKFDLNIRLWSIDDYGGWD
EAYKTYFDDGAMFDEIYGN

Sequences:

>Translated_339_residues
MRNIHKYTAAMLSAVMVVSLFLTGCSGKKDDGTVTITNVSYDPTREFYEKYNKIFESYYESEHGKRINVIQSHGGSGAQA
RSVVEGCNADVVTLALEHDIDLIQNTGLIEKGWIDEFPKSSAPYTSTIVFLVRKGNPKHISDWDDLVKKNVDVITPDPKS
SGGACWNFLAALSYAKTNYDSQKEQKTFMRKLYENVTIMDSGARGSTTTFVENGQGDVLIAWENEALTTMDSYPGKYEIV
NPSVSILAQPSVAIVDDNAKSNGTEAASKEYLQYLYSDEAQRLIGESGYRPTNQDILNQFSDKFDLNIRLWSIDDYGGWD
EAYKTYFDDGAMFDEIYGN
>Mature_339_residues
MRNIHKYTAAMLSAVMVVSLFLTGCSGKKDDGTVTITNVSYDPTREFYEKYNKIFESYYESEHGKRINVIQSHGGSGAQA
RSVVEGCNADVVTLALEHDIDLIQNTGLIEKGWIDEFPKSSAPYTSTIVFLVRKGNPKHISDWDDLVKKNVDVITPDPKS
SGGACWNFLAALSYAKTNYDSQKEQKTFMRKLYENVTIMDSGARGSTTTFVENGQGDVLIAWENEALTTMDSYPGKYEIV
NPSVSILAQPSVAIVDDNAKSNGTEAASKEYLQYLYSDEAQRLIGESGYRPTNQDILNQFSDKFDLNIRLWSIDDYGGWD
EAYKTYFDDGAMFDEIYGN

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=322, Percent_Identity=51.2422360248447, Blast_Score=330, Evalue=6e-92,
Organism=Escherichia coli, GI1788765, Length=305, Percent_Identity=40.327868852459, Blast_Score=233, Evalue=1e-62,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37914; Mature: 37914

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNIHKYTAAMLSAVMVVSLFLTGCSGKKDDGTVTITNVSYDPTREFYEKYNKIFESYYE
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
SEHGKRINVIQSHGGSGAQARSVVEGCNADVVTLALEHDIDLIQNTGLIEKGWIDEFPKS
HCCCCEEEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHCCCCCCCCCCCCCCCC
SAPYTSTIVFLVRKGNPKHISDWDDLVKKNVDVITPDPKSSGGACWNFLAALSYAKTNYD
CCCCCEEEEEEEECCCCCCCCCHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCC
SQKEQKTFMRKLYENVTIMDSGARGSTTTFVENGQGDVLIAWENEALTTMDSYPGKYEIV
CHHHHHHHHHHHHCCEEEEECCCCCCCEEEEECCCCCEEEEECCCCEEHHHCCCCEEEEE
NPSVSILAQPSVAIVDDNAKSNGTEAASKEYLQYLYSDEAQRLIGESGYRPTNQDILNQF
CCCEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
SDKFDLNIRLWSIDDYGGWDEAYKTYFDDGAMFDEIYGN
CCCEEEEEEEEEECCCCCHHHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure
MRNIHKYTAAMLSAVMVVSLFLTGCSGKKDDGTVTITNVSYDPTREFYEKYNKIFESYYE
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
SEHGKRINVIQSHGGSGAQARSVVEGCNADVVTLALEHDIDLIQNTGLIEKGWIDEFPKS
HCCCCEEEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHCCCCCCCCCCCCCCCC
SAPYTSTIVFLVRKGNPKHISDWDDLVKKNVDVITPDPKSSGGACWNFLAALSYAKTNYD
CCCCCEEEEEEEECCCCCCCCCHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCC
SQKEQKTFMRKLYENVTIMDSGARGSTTTFVENGQGDVLIAWENEALTTMDSYPGKYEIV
CHHHHHHHHHHHHCCEEEEECCCCCCCEEEEECCCCCEEEEECCCCEEHHHCCCCEEEEE
NPSVSILAQPSVAIVDDNAKSNGTEAASKEYLQYLYSDEAQRLIGESGYRPTNQDILNQF
CCCEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
SDKFDLNIRLWSIDDYGGWDEAYKTYFDDGAMFDEIYGN
CCCEEEEEEEEEECCCCCHHHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]