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The map label for this gene is cysU [H]

Identifier: 238924824

GI number: 238924824

Start: 2343415

End: 2344293

Strand: Reverse

Name: cysU [H]

Synonym: EUBREC_2475

Alternate gene names: 238924824

Gene position: 2344293-2343415 (Counterclockwise)

Preceding gene: 238924825

Following gene: 238924823

Centisome position: 67.96

GC content: 44.94

Gene sequence:

>879_bases
ATGAAATTTACGGCAACTGATAAAACAGCAAAAAAGAAAACTAAATCAAAGAACATAAAGACCAGAGTTATACCGGGCTA
CCATCTTTCGCTTGGGATAGTGGTCACGATGCTTTCAGTCATCGTACTCATTCCGCTGGCATCAGTGCTTGTGTATTCGC
TAAAGATATCACCCGGTGATTTTGTGGCACTTATCATGAAGGAGAATGTGAGGAATGCCTTTATCACAAGCATCACCTCG
TCATTTATCGCAGCGATTATAAATGTGGTATTTGGACTCATCGTCGCATGGACACTTGTGAAGTACGATTTCCCCGGAAA
ATGGCTGCTTGACGGACTCATAGAGCTTCCGTTTGCACTGCCGACAGCGGTTGCGGGCATCACGCTCTCAAAGCTTTACT
CGGGCACAGGCTTTTTTGGCAAGGGCTTAAGTAAGCTTGGAATAGATGTGGCTTACACACAGGCAGGAATAGTTGTGGCA
CTGGTTTTTGTAGGTATTCCCTTCGTGATCAGAGCAGTTCAGCCGGTACTGGAAAAGATGGATAATACATATGAGGAGGC
AGCATATATGCTTGGTGCCACACCCTCTAAGACCTTTTTTAAGGTAATACTTCCGGAGCTTCGTCCGGCACTCTTAACAG
GCTTTGGTCTTGCATTTGCCAGAGGCATAGGAGAGTACGGAAGCGTTATCTACATATCAGGCAACAGCGCAAAACACAGG
ACACAGGTGGTATCCTACGTCATCATGCAGAAGCTTGGCTATATAGATTATGCCAGCGCGACTGCCATAGCACTTGTGAT
GCTTGTCATATCATTTTTACTGCTGTTATTTGTAAACATAGTGCAGGTTAAACAGTCACAGCGCACTAATTTGTTGTAG

Upstream 100 bases:

>100_bases
TCCGACAAGTTTGATTTGAATATCAGGCTTTGGAGCATAGATGATTACGGCGGATGGGATGAAGCCTACAAGACATATTT
CGATGATGGAGCAATGTTCG

Downstream 100 bases:

>100_bases
AAGGCTGCATATAAATCAAATGCGATGCACACAAACAGGAAAGGAGCACAATGCGATGACCGAACAGGTAAAGCTTGAAA
GAAGAAAAAAGATCACAAAG

Product: sulfate ABC transporter, permease protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MKFTATDKTAKKKTKSKNIKTRVIPGYHLSLGIVVTMLSVIVLIPLASVLVYSLKISPGDFVALIMKENVRNAFITSITS
SFIAAIINVVFGLIVAWTLVKYDFPGKWLLDGLIELPFALPTAVAGITLSKLYSGTGFFGKGLSKLGIDVAYTQAGIVVA
LVFVGIPFVIRAVQPVLEKMDNTYEEAAYMLGATPSKTFFKVILPELRPALLTGFGLAFARGIGEYGSVIYISGNSAKHR
TQVVSYVIMQKLGYIDYASATAIALVMLVISFLLLLFVNIVQVKQSQRTNLL

Sequences:

>Translated_292_residues
MKFTATDKTAKKKTKSKNIKTRVIPGYHLSLGIVVTMLSVIVLIPLASVLVYSLKISPGDFVALIMKENVRNAFITSITS
SFIAAIINVVFGLIVAWTLVKYDFPGKWLLDGLIELPFALPTAVAGITLSKLYSGTGFFGKGLSKLGIDVAYTQAGIVVA
LVFVGIPFVIRAVQPVLEKMDNTYEEAAYMLGATPSKTFFKVILPELRPALLTGFGLAFARGIGEYGSVIYISGNSAKHR
TQVVSYVIMQKLGYIDYASATAIALVMLVISFLLLLFVNIVQVKQSQRTNLL
>Mature_292_residues
MKFTATDKTAKKKTKSKNIKTRVIPGYHLSLGIVVTMLSVIVLIPLASVLVYSLKISPGDFVALIMKENVRNAFITSITS
SFIAAIINVVFGLIVAWTLVKYDFPGKWLLDGLIELPFALPTAVAGITLSKLYSGTGFFGKGLSKLGIDVAYTQAGIVVA
LVFVGIPFVIRAVQPVLEKMDNTYEEAAYMLGATPSKTFFKVILPELRPALLTGFGLAFARGIGEYGSVIYISGNSAKHR
TQVVSYVIMQKLGYIDYASATAIALVMLVISFLLLLFVNIVQVKQSQRTNLL

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=267, Percent_Identity=47.940074906367, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI87082099, Length=260, Percent_Identity=31.5384615384615, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1786980, Length=216, Percent_Identity=34.2592592592593, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787369, Length=211, Percent_Identity=25.5924170616114, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31697; Mature: 31697

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]