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The map label for this gene is cysNC [H]

Identifier: 238924818

GI number: 238924818

Start: 2336713

End: 2338353

Strand: Reverse

Name: cysNC [H]

Synonym: EUBREC_2469

Alternate gene names: 238924818

Gene position: 2338353-2336713 (Counterclockwise)

Preceding gene: 238924819

Following gene: 238924817

Centisome position: 67.78

GC content: 46.44

Gene sequence:

>1641_bases
ATGAGCGGATTACTTAAATTTATTACATGCGGAAGCGTGGACGACGGAAAATCAACTCTGATCGGACATATATTATACGA
TTCAAAGCTGCTCTATGCAGATCAGGAAAAGGCATTGGAGCTTGACAGTAAGGTCGGAAGCCGTGGCGGCGCCATCGACT
ATTCACTCCTCCTCGACGGACTGATGGCAGAGAGAGAGCAGGGAATCACAATCGATGTGGCTTATCGTTACTTCACAACA
GATAAGAGAAGCTTTATCGTGGCAGATACACCGGGCCATGAGGAGTACACAAGAAACATGGCTGTAGGTGCATCCTTTGC
AGACCTTGCGGTAATTTTAATAGATGCAAAGCAGGGCGTATTGGTTCAGACAAGAAGACACGCAAGAATCTGCGCGCTCA
TGGGAATCAACTACTTTGTATTTGCTGTAAACAAGATGGATCTTGTAGGCTACGACGAGAAGCGTTTCGATGAAATCACA
AAGCAGATTATTGAGCTTACAAAGGAGCTTGAACTCAAAAATGTAGTAATAATTCCTGTTTCTGCAACAGAGGGTGACAA
TGTCACAACAAAGTCTGAGAATATCCCATGGTACAAGGGACCTGCTCTCTTATCATACCTTGAGGATGTTGACATAAAGG
ATGAGAACGAGGAAGAGGGCTTTTACATGCCTGTACAGAGAGTCTGCCGTCCTGACCACACCTTCCGTGGATTCCAGGGC
CAGATAGAGGCCGGAGAGGTAAAGGTTGGCGATGAAATCACCACACTGCCAAGCAATGAGACAGCACATGTAAAAAGCAT
CCATGTGGGTGATAAGCTAAGTGATAGCGCATTTACAGGCCAGCCGGTTACAATCCAGCTTGACAGAGAGGTGGACGTAT
CAAGAGGCTGCGTGCTCACAATCGGTTCAGGAGCAAAGGTGGCTTCATCCATCACAGCTACAATACTCTGGATGGATGAC
GATGAGCTCTTTAAGGGAAAGAATTTCTTCTTCAAGCTTGGTACCAAGTCAATACCGGGCATCGTAACAGAGATAGAGCA
TACCATCGACGTAAACACAGGTGAGGAAAAGCCTGCCGACAAGCTTAAAAAGAACGAGATAGCAGTTGTAAAGATTGCAT
TCTCGGACAAGATAGTCTGCGATAAGTTCAAAAATCACAAGACACTTGGTGAGTTCATCTTAATCGACCGTGTGACGGAT
ATGACATCTGCCTGTGGTGTGGTTGAAGAGGTACACACAGAGGAAAGCGGTCTCTACGAGGGCAGAGTAGACAGAAACGT
AAGAGCTGCAATCAAGGGTCAGAAGGCAATCACCGCCGTATTCGTAGACGGAGTGGATGGGGTAAACCGTGGCTTTGTGG
AGGATGTCGAGAAAGCCTTAAATATAGACGGACGCCATACTTATCTCTACGCACCAAAGGAGGGTGAGGACTTCGTAAAT
GTAGTCAAGCACCTAAGCCATGCCGGAATACTCGTACTTCTGCTCATCAGCCAAAAGCAGGAGAAGGAGCTTGCAGCCGA
CAAAGTTGAGTTCACAAAGGACTGGAACAAAAACGGCAGAGATGTGGACAAGGCTGCGGAGTTTATCAAAAAGCAGTCAG
TATATGACGGCGTGATCGTAAACACAAGTGAGTATATCTAA

Upstream 100 bases:

>100_bases
GAGACACTCAGTGCTGTTTCCTCAGAGAGAACCACCCGTGTCATAGACAACGAGGCGGCAGGCAGCATGGAAAGAAGAAA
GAGGGAGGGATATTTCTAAA

Downstream 100 bases:

>100_bases
TTAAAAAAGGGTACTTGATTTCTCAAGTACCCTCATGTATAATCTACTTAGAAAGTGAATCGGATATGACTCCGATTTGC
CCTCAGTGCTAAAATGTTAT

Product: sulfate adenylyltransferase, large subunit

Products: NA

Alternate protein names: Sulfate adenylyltransferase subunit 1; ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT; Adenylyl-sulfate kinase; APS kinase; ATP adenosine-5'-phosphosulfate 3'-phosphotransferase [H]

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MSGLLKFITCGSVDDGKSTLIGHILYDSKLLYADQEKALELDSKVGSRGGAIDYSLLLDGLMAEREQGITIDVAYRYFTT
DKRSFIVADTPGHEEYTRNMAVGASFADLAVILIDAKQGVLVQTRRHARICALMGINYFVFAVNKMDLVGYDEKRFDEIT
KQIIELTKELELKNVVIIPVSATEGDNVTTKSENIPWYKGPALLSYLEDVDIKDENEEEGFYMPVQRVCRPDHTFRGFQG
QIEAGEVKVGDEITTLPSNETAHVKSIHVGDKLSDSAFTGQPVTIQLDREVDVSRGCVLTIGSGAKVASSITATILWMDD
DELFKGKNFFFKLGTKSIPGIVTEIEHTIDVNTGEEKPADKLKKNEIAVVKIAFSDKIVCDKFKNHKTLGEFILIDRVTD
MTSACGVVEEVHTEESGLYEGRVDRNVRAAIKGQKAITAVFVDGVDGVNRGFVEDVEKALNIDGRHTYLYAPKEGEDFVN
VVKHLSHAGILVLLLISQKQEKELAADKVEFTKDWNKNGRDVDKAAEFIKKQSVYDGVIVNTSEYI

Sequences:

>Translated_546_residues
MSGLLKFITCGSVDDGKSTLIGHILYDSKLLYADQEKALELDSKVGSRGGAIDYSLLLDGLMAEREQGITIDVAYRYFTT
DKRSFIVADTPGHEEYTRNMAVGASFADLAVILIDAKQGVLVQTRRHARICALMGINYFVFAVNKMDLVGYDEKRFDEIT
KQIIELTKELELKNVVIIPVSATEGDNVTTKSENIPWYKGPALLSYLEDVDIKDENEEEGFYMPVQRVCRPDHTFRGFQG
QIEAGEVKVGDEITTLPSNETAHVKSIHVGDKLSDSAFTGQPVTIQLDREVDVSRGCVLTIGSGAKVASSITATILWMDD
DELFKGKNFFFKLGTKSIPGIVTEIEHTIDVNTGEEKPADKLKKNEIAVVKIAFSDKIVCDKFKNHKTLGEFILIDRVTD
MTSACGVVEEVHTEESGLYEGRVDRNVRAAIKGQKAITAVFVDGVDGVNRGFVEDVEKALNIDGRHTYLYAPKEGEDFVN
VVKHLSHAGILVLLLISQKQEKELAADKVEFTKDWNKNGRDVDKAAEFIKKQSVYDGVIVNTSEYI
>Mature_545_residues
SGLLKFITCGSVDDGKSTLIGHILYDSKLLYADQEKALELDSKVGSRGGAIDYSLLLDGLMAEREQGITIDVAYRYFTTD
KRSFIVADTPGHEEYTRNMAVGASFADLAVILIDAKQGVLVQTRRHARICALMGINYFVFAVNKMDLVGYDEKRFDEITK
QIIELTKELELKNVVIIPVSATEGDNVTTKSENIPWYKGPALLSYLEDVDIKDENEEEGFYMPVQRVCRPDHTFRGFQGQ
IEAGEVKVGDEITTLPSNETAHVKSIHVGDKLSDSAFTGQPVTIQLDREVDVSRGCVLTIGSGAKVASSITATILWMDDD
ELFKGKNFFFKLGTKSIPGIVTEIEHTIDVNTGEEKPADKLKKNEIAVVKIAFSDKIVCDKFKNHKTLGEFILIDRVTDM
TSACGVVEEVHTEESGLYEGRVDRNVRAAIKGQKAITAVFVDGVDGVNRGFVEDVEKALNIDGRHTYLYAPKEGEDFVNV
VKHLSHAGILVLLLISQKQEKELAADKVEFTKDWNKNGRDVDKAAEFIKKQSVYDGVIVNTSEYI

Specific function: APS kinase catalyzes the synthesis of activated sulfate [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the APS kinase family [H]

Homologues:

Organism=Homo sapiens, GI223555963, Length=429, Percent_Identity=31.4685314685315, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI5729864, Length=429, Percent_Identity=31.4685314685315, Blast_Score=186, Evalue=4e-47,
Organism=Homo sapiens, GI4503475, Length=466, Percent_Identity=27.8969957081545, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI4503471, Length=467, Percent_Identity=28.4796573875803, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI194018522, Length=409, Percent_Identity=25.6723716381418, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI194018520, Length=409, Percent_Identity=25.6723716381418, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI194097354, Length=409, Percent_Identity=25.6723716381418, Blast_Score=137, Evalue=4e-32,
Organism=Homo sapiens, GI46094014, Length=409, Percent_Identity=24.9388753056235, Blast_Score=132, Evalue=9e-31,
Organism=Homo sapiens, GI34147630, Length=337, Percent_Identity=26.1127596439169, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1789108, Length=410, Percent_Identity=44.6341463414634, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI2367247, Length=234, Percent_Identity=28.6324786324786, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1790412, Length=403, Percent_Identity=24.317617866005, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789737, Length=403, Percent_Identity=24.317617866005, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17552884, Length=466, Percent_Identity=28.5407725321888, Blast_Score=164, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI17569207, Length=466, Percent_Identity=28.5407725321888, Blast_Score=164, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI115532067, Length=432, Percent_Identity=28.2407407407407, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI115532065, Length=432, Percent_Identity=28.2407407407407, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI32566629, Length=414, Percent_Identity=27.7777777777778, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI32566303, Length=459, Percent_Identity=27.4509803921569, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI32566301, Length=148, Percent_Identity=34.4594594594595, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI25141371, Length=254, Percent_Identity=29.1338582677165, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556456, Length=339, Percent_Identity=27.4336283185841, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325337, Length=444, Percent_Identity=28.8288288288288, Blast_Score=167, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6319594, Length=444, Percent_Identity=28.8288288288288, Blast_Score=167, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6322937, Length=404, Percent_Identity=27.2277227722772, Blast_Score=153, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6320377, Length=423, Percent_Identity=28.8416075650118, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324761, Length=337, Percent_Identity=28.7833827893175, Blast_Score=98, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24652838, Length=468, Percent_Identity=28.4188034188034, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI17137572, Length=468, Percent_Identity=28.4188034188034, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI45553807, Length=448, Percent_Identity=27.6785714285714, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI45553816, Length=448, Percent_Identity=27.6785714285714, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24651721, Length=448, Percent_Identity=27.6785714285714, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI17864154, Length=448, Percent_Identity=27.6785714285714, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI45550900, Length=432, Percent_Identity=27.7777777777778, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI17137380, Length=406, Percent_Identity=26.8472906403941, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI281363316, Length=333, Percent_Identity=27.3273273273273, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17864358, Length=333, Percent_Identity=27.3273273273273, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19921738, Length=203, Percent_Identity=29.5566502463054, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002891
- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF01583 APS_kinase; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4; =2.7.1.25 [H]

Molecular weight: Translated: 60543; Mature: 60412

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLLKFITCGSVDDGKSTLIGHILYDSKLLYADQEKALELDSKVGSRGGAIDYSLLLDG
CCCCEEEEECCCCCCCCHHEEEEEEECCEEEEECCHHHHHHHHHCCCCCCCEEHHHHHHH
LMAEREQGITIDVAYRYFTTDKRSFIVADTPGHEEYTRNMAVGASFADLAVILIDAKQGV
HHHHHHCCCEEEEEEEEEECCCCEEEEEECCCCHHHHHHHCCCCCHHCEEEEEEECCCCE
LVQTRRHARICALMGINYFVFAVNKMDLVGYDEKRFDEITKQIIELTKELELKNVVIIPV
EEEECCCHHEEEHHCCEEEEEEEECEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEEE
SATEGDNVTTKSENIPWYKGPALLSYLEDVDIKDENEEEGFYMPVQRVCRPDHTFRGFQG
ECCCCCCCEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEECCHHHHCCCCCCCCCCCC
QIEAGEVKVGDEITTLPSNETAHVKSIHVGDKLSDSAFTGQPVTIQLDREVDVSRGCVLT
CEECCCEEECCCEEECCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCEEEE
IGSGAKVASSITATILWMDDDELFKGKNFFFKLGTKSIPGIVTEIEHTIDVNTGEEKPAD
ECCCCHHCCCEEEEEEEECCHHHHCCCCEEEEECCCCCCCHHHEEEEEEECCCCCCCCHH
KLKKNEIAVVKIAFSDKIVCDKFKNHKTLGEFILIDRVTDMTSACGVVEEVHTEESGLYE
HHCCCCEEEEEEEECCCEEHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCC
GRVDRNVRAAIKGQKAITAVFVDGVDGVNRGFVEDVEKALNIDGRHTYLYAPKEGEDFVN
CCCCCCHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHH
VVKHLSHAGILVLLLISQKQEKELAADKVEFTKDWNKNGRDVDKAAEFIKKQSVYDGVIV
HHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEE
NTSEYI
CCCCCC
>Mature Secondary Structure 
SGLLKFITCGSVDDGKSTLIGHILYDSKLLYADQEKALELDSKVGSRGGAIDYSLLLDG
CCCEEEEECCCCCCCCHHEEEEEEECCEEEEECCHHHHHHHHHCCCCCCCEEHHHHHHH
LMAEREQGITIDVAYRYFTTDKRSFIVADTPGHEEYTRNMAVGASFADLAVILIDAKQGV
HHHHHHCCCEEEEEEEEEECCCCEEEEEECCCCHHHHHHHCCCCCHHCEEEEEEECCCCE
LVQTRRHARICALMGINYFVFAVNKMDLVGYDEKRFDEITKQIIELTKELELKNVVIIPV
EEEECCCHHEEEHHCCEEEEEEEECEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEEE
SATEGDNVTTKSENIPWYKGPALLSYLEDVDIKDENEEEGFYMPVQRVCRPDHTFRGFQG
ECCCCCCCEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEECCHHHHCCCCCCCCCCCC
QIEAGEVKVGDEITTLPSNETAHVKSIHVGDKLSDSAFTGQPVTIQLDREVDVSRGCVLT
CEECCCEEECCCEEECCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCEEEE
IGSGAKVASSITATILWMDDDELFKGKNFFFKLGTKSIPGIVTEIEHTIDVNTGEEKPAD
ECCCCHHCCCEEEEEEEECCHHHHCCCCEEEEECCCCCCCHHHEEEEEEECCCCCCCCHH
KLKKNEIAVVKIAFSDKIVCDKFKNHKTLGEFILIDRVTDMTSACGVVEEVHTEESGLYE
HHCCCCEEEEEEEECCCEEHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCC
GRVDRNVRAAIKGQKAITAVFVDGVDGVNRGFVEDVEKALNIDGRHTYLYAPKEGEDFVN
CCCCCCHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHH
VVKHLSHAGILVLLLISQKQEKELAADKVEFTKDWNKNGRDVDKAAEFIKKQSVYDGVIV
HHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEE
NTSEYI
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]