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The map label for this gene is pyrE

Identifier: 238924817

GI number: 238924817

Start: 2335754

End: 2336434

Strand: Reverse

Name: pyrE

Synonym: EUBREC_2468

Alternate gene names: 238924817

Gene position: 2336434-2335754 (Counterclockwise)

Preceding gene: 238924818

Following gene: 238924816

Centisome position: 67.73

GC content: 46.99

Gene sequence:

>681_bases
ATGGAAAGCTACAAGAGCGAATTTATAGACTTTATGGTGGAGAGTGACGTGCTGAAATTTGGTGATTTCACATTAAAGAG
CGGTCGTAAATCACCATTTTTCATGAACGCGGGAGCATATGTGACAGGCTCACAGCTTAAGAGACTCGGAGAGTACTACG
CAAAGGCTATCCATGCAAACTACGGAGATGACTTTGATGTATTATTCGGACCTGCATACAAGGGAATTCCGCTTGCAGTG
GTAACAGCCATCGCATACCATGAGCTCTATGGCAAAGAGGTGCGCTACTGCTCAGACAGAAAAGAGGCAAAGGATCACGG
TGCAGACAAGGGAGGCTTCCTTGGAAGCAAGCTTAAGGATGGCGACAGAGTAGTCATGATAGAGGATGTCACCACATCAG
GCAAATCAATGGAGGAGACAGTACCAAAGGTAAGAGGAGCTGCTGATGTCACAATCGTAGGCCTTATGGTGAGCCTTAAC
AGAATGGAAGTCGGACAGGGCGGCAAGGTGAGCGCGCTCGACGAAATCCATGAGAAGTACGGCTTTGAGGGCAAGGCAAT
CGTTACAATGGAAGAGGTCACAGAGTATCTCTACAACAGAGAGCATGATGGCAGAGTAGTCATTGATGACACAATCAAAG
CAGCTATAGATGAGTACTATAAGCAGTACGGCTGCAAATAG

Upstream 100 bases:

>100_bases
CGCAAAAACAACTAATATTTCCACGTTGAAAAGCACGCCCCAATGTGGTAAAATTGACAAAGATAATTTAAAGAAAAACA
CACAGGAGGATTAACATACA

Downstream 100 bases:

>100_bases
GAGAATATAAATTGCTTGGCAGAAGAAAACGTAACAGGTATAAGTTTACGGAAAAGAAGCAATCTAAGAGGGGGATAGCT
GCACTTGTTGTGGCTATCCT

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MESYKSEFIDFMVESDVLKFGDFTLKSGRKSPFFMNAGAYVTGSQLKRLGEYYAKAIHANYGDDFDVLFGPAYKGIPLAV
VTAIAYHELYGKEVRYCSDRKEAKDHGADKGGFLGSKLKDGDRVVMIEDVTTSGKSMEETVPKVRGAADVTIVGLMVSLN
RMEVGQGGKVSALDEIHEKYGFEGKAIVTMEEVTEYLYNREHDGRVVIDDTIKAAIDEYYKQYGCK

Sequences:

>Translated_226_residues
MESYKSEFIDFMVESDVLKFGDFTLKSGRKSPFFMNAGAYVTGSQLKRLGEYYAKAIHANYGDDFDVLFGPAYKGIPLAV
VTAIAYHELYGKEVRYCSDRKEAKDHGADKGGFLGSKLKDGDRVVMIEDVTTSGKSMEETVPKVRGAADVTIVGLMVSLN
RMEVGQGGKVSALDEIHEKYGFEGKAIVTMEEVTEYLYNREHDGRVVIDDTIKAAIDEYYKQYGCK
>Mature_226_residues
MESYKSEFIDFMVESDVLKFGDFTLKSGRKSPFFMNAGAYVTGSQLKRLGEYYAKAIHANYGDDFDVLFGPAYKGIPLAV
VTAIAYHELYGKEVRYCSDRKEAKDHGADKGGFLGSKLKDGDRVVMIEDVTTSGKSMEETVPKVRGAADVTIVGLMVSLN
RMEVGQGGKVSALDEIHEKYGFEGKAIVTMEEVTEYLYNREHDGRVVIDDTIKAAIDEYYKQYGCK

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=183, Percent_Identity=29.5081967213115, Blast_Score=68, Evalue=6e-12,
Organism=Escherichia coli, GI1790073, Length=224, Percent_Identity=41.0714285714286, Blast_Score=172, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6323530, Length=231, Percent_Identity=36.7965367965368, Blast_Score=142, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6323927, Length=230, Percent_Identity=35.2173913043478, Blast_Score=136, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_EUBR3 (C4ZF78)

Other databases:

- EMBL:   CP001107
- RefSeq:   YP_002938333.1
- ProteinModelPortal:   C4ZF78
- GeneID:   7966173
- GenomeReviews:   CP001107_GR
- KEGG:   ere:EUBREC_2468
- ProtClustDB:   PRK00455
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 25162; Mature: 25162

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 26-26 BINDING 100-100 BINDING 101-101 BINDING 104-104 BINDING 106-106 BINDING 132-132 BINDING 161-161

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESYKSEFIDFMVESDVLKFGDFTLKSGRKSPFFMNAGAYVTGSQLKRLGEYYAKAIHAN
CCCHHHHHHHHHHHHHHHHHCCEEECCCCCCCEEECCCCEEEHHHHHHHHHHHHHHHHCC
YGDDFDVLFGPAYKGIPLAVVTAIAYHELYGKEVRYCSDRKEAKDHGADKGGFLGSKLKD
CCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GDRVVMIEDVTTSGKSMEETVPKVRGAADVTIVGLMVSLNRMEVGQGGKVSALDEIHEKY
CCEEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
GFEGKAIVTMEEVTEYLYNREHDGRVVIDDTIKAAIDEYYKQYGCK
CCCCCEEEEHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MESYKSEFIDFMVESDVLKFGDFTLKSGRKSPFFMNAGAYVTGSQLKRLGEYYAKAIHAN
CCCHHHHHHHHHHHHHHHHHCCEEECCCCCCCEEECCCCEEEHHHHHHHHHHHHHHHHCC
YGDDFDVLFGPAYKGIPLAVVTAIAYHELYGKEVRYCSDRKEAKDHGADKGGFLGSKLKD
CCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GDRVVMIEDVTTSGKSMEETVPKVRGAADVTIVGLMVSLNRMEVGQGGKVSALDEIHEKY
CCEEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
GFEGKAIVTMEEVTEYLYNREHDGRVVIDDTIKAAIDEYYKQYGCK
CCCCCEEEEHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA