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The map label for this gene is cysD [H]

Identifier: 238924819

GI number: 238924819

Start: 2338355

End: 2339266

Strand: Reverse

Name: cysD [H]

Synonym: EUBREC_2470

Alternate gene names: 238924819

Gene position: 2339266-2338355 (Counterclockwise)

Preceding gene: 238924820

Following gene: 238924818

Centisome position: 67.81

GC content: 46.16

Gene sequence:

>912_bases
ATGGTAACAGACATGGGTGAATTATCACATCTTGACGAACTCGAAGCAGAGGCAATATACATAATACGTGAAGTAGCCGC
AGAGTGCGAGAAGCCGGTTATGTTGTACTCAATTGGAAAGGACAGCTCGGTAATGCTTCATCTTGCAATGAAGGCATTTT
ATCCGGAGAAGCCGCCATTTCCGTTTTTACATGTAAATACAACCTGGAAATTCCATGAGATGATAGAATTCAGAGATCGC
ATTGCTAAGGAAAAGGGCATCGAGATGCTTGAATATATAAATCAGGATGGCGTAAAGCAGGGCATCAATCCATTTGACCA
CGGCTCAGCATACACAGATATCATGAAGACACAGGCGCTCAAGCAGGCGCTCGACAAATACGGCTTTACGGCAGCGTTTG
GCGGCGGCCGAAGAGACGAGGAGAAATCAAGAGCCAAGGAGAGAATCTTCTCCTTCAGAAATGAGAACCACGCATGGGAT
CCAAAGAACCAGCGCCCGGAGATGTGGAAGCTCTACAACTCAAGAATTAAAAAGGGCCAGGAGGTGCGAGTTTTCCCACT
TTCAAACTGGACAGAGAAGGATATCTGGCAGTATATAAAGAGAGAGAATATCGAGATTCCTTCACTTTACTTTGCAAAGG
AAAGACCTGTTGTATACAGAGACGGCAACATCATCATGGTAGATGACGACAGAATGAAGCTTCGTCCGGGTGAGAAAATC
CAGATGAAGAGTGTACGTTTCAGAACTCTCGGCTGCTATCCACTGACAGGTGGTGTGGAGTCGACAGCGGATACACTTGA
TGAGATTATTGATGAGACACTCAGTGCTGTTTCCTCAGAGAGAACCACCCGTGTCATAGACAACGAGGCGGCAGGCAGCA
TGGAAAGAAGAAAGAGGGAGGGATATTTCTAA

Upstream 100 bases:

>100_bases
GCACACCGGACACGCTGACCTGGGATATCAAAAAGCGCGACGGAAGCAAGGAAGAGATTCTTATCAACAAGAAGGAATCC
AATAAATATTAAAGAAAAGG

Downstream 100 bases:

>100_bases
AATGAGCGGATTACTTAAATTTATTACATGCGGAAGCGTGGACGACGGAAAATCAACTCTGATCGGACATATATTATACG
ATTCAAAGCTGCTCTATGCA

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MVTDMGELSHLDELEAEAIYIIREVAAECEKPVMLYSIGKDSSVMLHLAMKAFYPEKPPFPFLHVNTTWKFHEMIEFRDR
IAKEKGIEMLEYINQDGVKQGINPFDHGSAYTDIMKTQALKQALDKYGFTAAFGGGRRDEEKSRAKERIFSFRNENHAWD
PKNQRPEMWKLYNSRIKKGQEVRVFPLSNWTEKDIWQYIKRENIEIPSLYFAKERPVVYRDGNIIMVDDDRMKLRPGEKI
QMKSVRFRTLGCYPLTGGVESTADTLDEIIDETLSAVSSERTTRVIDNEAAGSMERRKREGYF

Sequences:

>Translated_303_residues
MVTDMGELSHLDELEAEAIYIIREVAAECEKPVMLYSIGKDSSVMLHLAMKAFYPEKPPFPFLHVNTTWKFHEMIEFRDR
IAKEKGIEMLEYINQDGVKQGINPFDHGSAYTDIMKTQALKQALDKYGFTAAFGGGRRDEEKSRAKERIFSFRNENHAWD
PKNQRPEMWKLYNSRIKKGQEVRVFPLSNWTEKDIWQYIKRENIEIPSLYFAKERPVVYRDGNIIMVDDDRMKLRPGEKI
QMKSVRFRTLGCYPLTGGVESTADTLDEIIDETLSAVSSERTTRVIDNEAAGSMERRKREGYF
>Mature_303_residues
MVTDMGELSHLDELEAEAIYIIREVAAECEKPVMLYSIGKDSSVMLHLAMKAFYPEKPPFPFLHVNTTWKFHEMIEFRDR
IAKEKGIEMLEYINQDGVKQGINPFDHGSAYTDIMKTQALKQALDKYGFTAAFGGGRRDEEKSRAKERIFSFRNENHAWD
PKNQRPEMWKLYNSRIKKGQEVRVFPLSNWTEKDIWQYIKRENIEIPSLYFAKERPVVYRDGNIIMVDDDRMKLRPGEKI
QMKSVRFRTLGCYPLTGGVESTADTLDEIIDETLSAVSSERTTRVIDNEAAGSMERRKREGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=301, Percent_Identity=68.1063122923588, Blast_Score=422, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 35303; Mature: 35303

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTDMGELSHLDELEAEAIYIIREVAAECEKPVMLYSIGKDSSVMLHLAMKAFYPEKPPF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCC
PFLHVNTTWKFHEMIEFRDRIAKEKGIEMLEYINQDGVKQGINPFDHGSAYTDIMKTQAL
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHH
KQALDKYGFTAAFGGGRRDEEKSRAKERIFSFRNENHAWDPKNQRPEMWKLYNSRIKKGQ
HHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCC
EVRVFPLSNWTEKDIWQYIKRENIEIPSLYFAKERPVVYRDGNIIMVDDDRMKLRPGEKI
CEEEEECCCCCHHHHHHHHHHCCCCCCCEEEECCCCEEEECCCEEEEECCCEECCCCCCE
QMKSVRFRTLGCYPLTGGVESTADTLDEIIDETLSAVSSERTTRVIDNEAAGSMERRKRE
EHHHEEEEEECCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHC
GYF
CCC
>Mature Secondary Structure
MVTDMGELSHLDELEAEAIYIIREVAAECEKPVMLYSIGKDSSVMLHLAMKAFYPEKPPF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCC
PFLHVNTTWKFHEMIEFRDRIAKEKGIEMLEYINQDGVKQGINPFDHGSAYTDIMKTQAL
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHH
KQALDKYGFTAAFGGGRRDEEKSRAKERIFSFRNENHAWDPKNQRPEMWKLYNSRIKKGQ
HHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCC
EVRVFPLSNWTEKDIWQYIKRENIEIPSLYFAKERPVVYRDGNIIMVDDDRMKLRPGEKI
CEEEEECCCCCHHHHHHHHHHCCCCCCCEEEECCCCEEEECCCEEEEECCCEECCCCCCE
QMKSVRFRTLGCYPLTGGVESTADTLDEIIDETLSAVSSERTTRVIDNEAAGSMERRKRE
EHHHEEEEEECCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHC
GYF
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]