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The map label for this gene is hisZ [H]

Identifier: 238924047

GI number: 238924047

Start: 1567876

End: 1569123

Strand: Direct

Name: hisZ [H]

Synonym: EUBREC_1685

Alternate gene names: 238924047

Gene position: 1567876-1569123 (Clockwise)

Preceding gene: 238924046

Following gene: 238924048

Centisome position: 45.45

GC content: 41.51

Gene sequence:

>1248_bases
ATGGTAGCATATTACCACGATAACACTTTACTACAAGAAAGCGAGATTTTACAGATTATGGAAAATCAATTATTACACAC
TCCGGACGGAGTAAGAGACATATATAACGGAGAATGCAAAAAAAAGCTGTATTTGCAGGATAAGCTGCACCGTACACTTT
TAAAATACGGATATCATGATATCATGACACCTACGTTTGAGTTTTTTAATATTTTTGGAAGCGATGTCGGAACAACCCCT
TCAAAGGATTTATATAAGTTTTTTGACAAGGAGGGCAATACGCTTGTTCTTCGTCCTGATTTCACACCTTCTATTGCAAG
AAGTGCGGCTAAGTACTATATAGAGGACGATATGCCTGTCAAGCTGTGTTATCTTGGCAATACTTTTATCAATTCATCGG
ATTATCAGGGCAGGCTCAAGGAAAGCACACAGTGCGGTGCAGAGCTGATAGGTGATGGCTCTATCTCTGCAGATGCCGAG
ATACTTTCGATGACGGTTGAGTCGATGCTTGAGAGCGGACTTAAGAATTTCCAGATTTCAGTCGGACATTCGCAGTTTTT
TTACGGTCTCACAAAGGCAGCAGGGCTGTCAGGGGAGCAGGAGGAAAACCTAAAAGAGCTCATAGCAAATAAGAATTTCT
TCGGTGTGGAGGAGTTTGTTGACAGTCTTTCGATGAACGACAAGCTAAGGAAGCTGTTTGGACTGTTAGACAATTTCGAT
TTACAGGATGAGCAGCTTATGGAGGCTTTAAAGCTTGCGGAGGGATATGATGAGATATTATCATCTATTGATACTCTGCT
TAAGCTTAGAGAATATTTAAAGGCGTACGGCATCGAGAAATATATTTCGTATGAGCTTGGACTTATCAGTGACTACACAT
ATTACACTGGTATCATTTTCCAGGGCTACACATATGGTACAGGAGAGCCTATTGTAAAGGGAGGCCGTTATGACAAGCTG
CTTTCACACTTTGGAAAGGACGCTGCTGCAATCGGCTTTGCAATTGTTGTGGATCAGCTCATGGCAGCATTAAGCCGTCA
GGATATTGATGTGCCTGTCATTGAGAATTCCTCACTTATAGTATTTGAGGAGGCTGCATACCATGCGGCAATCAAGCGCT
CTATGGAGCTTCGAAGAGATGGGGTAAATACACAGATGGTGCTTAAGGACAAAAATAAAACGAAAGAGGACTATGCTTCA
TATGCGGTAGACAACCGTATAAACAGAGTTGAGTTTATAGAGGAATAA

Upstream 100 bases:

>100_bases
GCCGGCATTTTTCCTCCCGGAGGTGCCCGGAATACTTGTGCCGTATCTGAACGCTCTTCAGCTTGACTTAAATTGCAGGG
CTTGACAGTATTACTATCGT

Downstream 100 bases:

>100_bases
AAGATGAGTGATGACAGATATTTAACCTTTGCCCTGGGAAAGGGCAGACTTGCAAAAAAGACACTGCAGCTTTTTGAGCA
GATAGGCATCACCTGTGAGG

Product: histidine--tRNA ligase

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MVAYYHDNTLLQESEILQIMENQLLHTPDGVRDIYNGECKKKLYLQDKLHRTLLKYGYHDIMTPTFEFFNIFGSDVGTTP
SKDLYKFFDKEGNTLVLRPDFTPSIARSAAKYYIEDDMPVKLCYLGNTFINSSDYQGRLKESTQCGAELIGDGSISADAE
ILSMTVESMLESGLKNFQISVGHSQFFYGLTKAAGLSGEQEENLKELIANKNFFGVEEFVDSLSMNDKLRKLFGLLDNFD
LQDEQLMEALKLAEGYDEILSSIDTLLKLREYLKAYGIEKYISYELGLISDYTYYTGIIFQGYTYGTGEPIVKGGRYDKL
LSHFGKDAAAIGFAIVVDQLMAALSRQDIDVPVIENSSLIVFEEAAYHAAIKRSMELRRDGVNTQMVLKDKNKTKEDYAS
YAVDNRINRVEFIEE

Sequences:

>Translated_415_residues
MVAYYHDNTLLQESEILQIMENQLLHTPDGVRDIYNGECKKKLYLQDKLHRTLLKYGYHDIMTPTFEFFNIFGSDVGTTP
SKDLYKFFDKEGNTLVLRPDFTPSIARSAAKYYIEDDMPVKLCYLGNTFINSSDYQGRLKESTQCGAELIGDGSISADAE
ILSMTVESMLESGLKNFQISVGHSQFFYGLTKAAGLSGEQEENLKELIANKNFFGVEEFVDSLSMNDKLRKLFGLLDNFD
LQDEQLMEALKLAEGYDEILSSIDTLLKLREYLKAYGIEKYISYELGLISDYTYYTGIIFQGYTYGTGEPIVKGGRYDKL
LSHFGKDAAAIGFAIVVDQLMAALSRQDIDVPVIENSSLIVFEEAAYHAAIKRSMELRRDGVNTQMVLKDKNKTKEDYAS
YAVDNRINRVEFIEE
>Mature_415_residues
MVAYYHDNTLLQESEILQIMENQLLHTPDGVRDIYNGECKKKLYLQDKLHRTLLKYGYHDIMTPTFEFFNIFGSDVGTTP
SKDLYKFFDKEGNTLVLRPDFTPSIARSAAKYYIEDDMPVKLCYLGNTFINSSDYQGRLKESTQCGAELIGDGSISADAE
ILSMTVESMLESGLKNFQISVGHSQFFYGLTKAAGLSGEQEENLKELIANKNFFGVEEFVDSLSMNDKLRKLFGLLDNFD
LQDEQLMEALKLAEGYDEILSSIDTLLKLREYLKAYGIEKYISYELGLISDYTYYTGIIFQGYTYGTGEPIVKGGRYDKL
LSHFGKDAAAIGFAIVVDQLMAALSRQDIDVPVIENSSLIVFEEAAYHAAIKRSMELRRDGVNTQMVLKDKNKTKEDYAS
YAVDNRINRVEFIEE

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine [H]

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=347, Percent_Identity=24.7838616714697, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI1788861, Length=321, Percent_Identity=24.9221183800623, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71993693, Length=424, Percent_Identity=23.8207547169811, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71993686, Length=424, Percent_Identity=23.8207547169811, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6325290, Length=392, Percent_Identity=24.7448979591837, Blast_Score=90, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24643061, Length=357, Percent_Identity=24.3697478991597, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24643059, Length=375, Percent_Identity=23.7333333333333, Blast_Score=78, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR004517
- InterPro:   IPR004516 [H]

Pfam domain/function: PF00587 tRNA-synt_2b [H]

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 47111; Mature: 47111

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAYYHDNTLLQESEILQIMENQLLHTPDGVRDIYNGECKKKLYLQDKLHRTLLKYGYHD
CEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHH
IMTPTFEFFNIFGSDVGTTPSKDLYKFFDKEGNTLVLRPDFTPSIARSAAKYYIEDDMPV
HCCHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHEECCCCCE
KLCYLGNTFINSSDYQGRLKESTQCGAELIGDGSISADAEILSMTVESMLESGLKNFQIS
EEEEECCHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCHHHEEEE
VGHSQFFYGLTKAAGLSGEQEENLKELIANKNFFGVEEFVDSLSMNDKLRKLFGLLDNFD
ECCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCC
LQDEQLMEALKLAEGYDEILSSIDTLLKLREYLKAYGIEKYISYELGLISDYTYYTGIIF
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHEEE
QGYTYGTGEPIVKGGRYDKLLSHFGKDAAAIGFAIVVDQLMAALSRQDIDVPVIENSSLI
ECEECCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEE
VFEEAAYHAAIKRSMELRRDGVNTQMVLKDKNKTKEDYASYAVDNRINRVEFIEE
EEEHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCHHHCCC
>Mature Secondary Structure
MVAYYHDNTLLQESEILQIMENQLLHTPDGVRDIYNGECKKKLYLQDKLHRTLLKYGYHD
CEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHH
IMTPTFEFFNIFGSDVGTTPSKDLYKFFDKEGNTLVLRPDFTPSIARSAAKYYIEDDMPV
HCCHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHEECCCCCE
KLCYLGNTFINSSDYQGRLKESTQCGAELIGDGSISADAEILSMTVESMLESGLKNFQIS
EEEEECCHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCHHHEEEE
VGHSQFFYGLTKAAGLSGEQEENLKELIANKNFFGVEEFVDSLSMNDKLRKLFGLLDNFD
ECCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCC
LQDEQLMEALKLAEGYDEILSSIDTLLKLREYLKAYGIEKYISYELGLISDYTYYTGIIF
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHEEE
QGYTYGTGEPIVKGGRYDKLLSHFGKDAAAIGFAIVVDQLMAALSRQDIDVPVIENSSLI
ECEECCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEE
VFEEAAYHAAIKRSMELRRDGVNTQMVLKDKNKTKEDYASYAVDNRINRVEFIEE
EEEHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA