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The map label for this gene is pgk [H]

Identifier: 238923895

GI number: 238923895

Start: 1407043

End: 1408275

Strand: Direct

Name: pgk [H]

Synonym: EUBREC_1523

Alternate gene names: 238923895

Gene position: 1407043-1408275 (Clockwise)

Preceding gene: 238923894

Following gene: 238923896

Centisome position: 40.79

GC content: 44.85

Gene sequence:

>1233_bases
ATGTCATTAAACAAGAAATCAATTGATGATATCAACGTAAAAGGCAAGAGAGTTCTCGTTAGATGTGACTTCAACGTGCC
ACTTAAGGACGGAAAAATCACAGATGAGAACCGTATCAACGGAGCTCTTCCAACAATCCAGAAATTAATCAAGGACGGTG
GAAAGGTTATCCTTTGCTCACACCTTGGAAAAGTTAAAAACGGACCAAACGAGGGTGAGTCACTTGCTCCTGTAGCTGCA
GCTCTTGAGGCTAAGCTTGGACAGAAGGTTACATTTGTAGCTGACTACAACGTAACAGGAGAGGCTGCAACAAAGGCTGT
TTCAGAGATGAAAGAGGGAGATGTAGTTCTTCTTCAGAATACACGTTTCAGAGGCAAAGAGGAGACAAAGCCACAGGAAG
CCGGAGATGCATTCGCAAAAGAGCTTGCTGACCTTGCTGATGTATATGTATGTGACGCTTTCGGTTCTGCTCATAGAGCA
CATGCTTCTGTAGCAGGTGTTACAAAGTTCATCACAGCTAAGGGTGGAGAGAATGTTGTTGGATACCTTATGCAGAAGGA
AATTGACTTCCTTGGCAAGGCAGTAGAGAATCCTGTACGTCCTTTCGTAGCTATTCTTGGTGGAGCTAAAGTAGCTGACA
AGCTCAATGTTATCGACAATCTCCTCGAGAAGGCTGATACACTTATCATCGGTGGTGGAATGGCATACACATTCCTTAAA
GCACAGGGCTATGAGATTGGTATCTCAATGCTTGATGAGACAAAGATTGATTACTGTAAAGAGATGCTTGCAAAAGCAGA
GAAACTTGGAAAGAAGATTCTTCTTCCTGTTGATGCAGTTACAATCAAGGATTTCCCAAATCCAATCGATGCTCCTGTAG
AGACAGAGACATATGATTATGACAAGATGCCTGCAGACCGTGAGGGCTGTGATATCGGACCTAAGACACGTGAGCTTTTC
GCTGACGCTGTTGCATCAGCTAAAACTGTTGTATGGAACGGACCAATGGGTGTATTTGAGAACCCAACACTTGCAGCAGG
TACACTTGCAGTAGCAGAGGCACTTGCTAAATCAGATGCTATCACAATCATCGGTGGTGGAGATTCAGCTGCAGCAGTTA
ACCAGATGGGACTTGGCGACAAGATGAGCCACATCTCAACAGGTGGCGGTGCTTCCCTTGAGTTCCTTGAGGGCAAGGAG
CTTCCTGGCGTAGCTTGTGCTGATGATAAATAA

Upstream 100 bases:

>100_bases
CTTTTGAATATCTGTGAATTGTAGAATTAATTATATTTTTTAATATGCACAATATGGCGTGGCCACATGGCTTATGCCTT
GAAATAAGGGAGGACATATT

Downstream 100 bases:

>100_bases
GCCGAAAGCAGCTTAATGAAATCAAGCCGCAGGCAAAAAATATTTGTATATAAATACAAGGAGATATAAAATGGCAAGAA
AGAAAATTGTAGCCGGCAAC

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MSLNKKSIDDINVKGKRVLVRCDFNVPLKDGKITDENRINGALPTIQKLIKDGGKVILCSHLGKVKNGPNEGESLAPVAA
ALEAKLGQKVTFVADYNVTGEAATKAVSEMKEGDVVLLQNTRFRGKEETKPQEAGDAFAKELADLADVYVCDAFGSAHRA
HASVAGVTKFITAKGGENVVGYLMQKEIDFLGKAVENPVRPFVAILGGAKVADKLNVIDNLLEKADTLIIGGGMAYTFLK
AQGYEIGISMLDETKIDYCKEMLAKAEKLGKKILLPVDAVTIKDFPNPIDAPVETETYDYDKMPADREGCDIGPKTRELF
ADAVASAKTVVWNGPMGVFENPTLAAGTLAVAEALAKSDAITIIGGGDSAAAVNQMGLGDKMSHISTGGGASLEFLEGKE
LPGVACADDK

Sequences:

>Translated_410_residues
MSLNKKSIDDINVKGKRVLVRCDFNVPLKDGKITDENRINGALPTIQKLIKDGGKVILCSHLGKVKNGPNEGESLAPVAA
ALEAKLGQKVTFVADYNVTGEAATKAVSEMKEGDVVLLQNTRFRGKEETKPQEAGDAFAKELADLADVYVCDAFGSAHRA
HASVAGVTKFITAKGGENVVGYLMQKEIDFLGKAVENPVRPFVAILGGAKVADKLNVIDNLLEKADTLIIGGGMAYTFLK
AQGYEIGISMLDETKIDYCKEMLAKAEKLGKKILLPVDAVTIKDFPNPIDAPVETETYDYDKMPADREGCDIGPKTRELF
ADAVASAKTVVWNGPMGVFENPTLAAGTLAVAEALAKSDAITIIGGGDSAAAVNQMGLGDKMSHISTGGGASLEFLEGKE
LPGVACADDK
>Mature_409_residues
SLNKKSIDDINVKGKRVLVRCDFNVPLKDGKITDENRINGALPTIQKLIKDGGKVILCSHLGKVKNGPNEGESLAPVAAA
LEAKLGQKVTFVADYNVTGEAATKAVSEMKEGDVVLLQNTRFRGKEETKPQEAGDAFAKELADLADVYVCDAFGSAHRAH
ASVAGVTKFITAKGGENVVGYLMQKEIDFLGKAVENPVRPFVAILGGAKVADKLNVIDNLLEKADTLIIGGGMAYTFLKA
QGYEIGISMLDETKIDYCKEMLAKAEKLGKKILLPVDAVTIKDFPNPIDAPVETETYDYDKMPADREGCDIGPKTRELFA
DAVASAKTVVWNGPMGVFENPTLAAGTLAVAEALAKSDAITIIGGGDSAAAVNQMGLGDKMSHISTGGGASLEFLEGKEL
PGVACADDK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=423, Percent_Identity=46.8085106382979, Blast_Score=335, Evalue=4e-92,
Organism=Homo sapiens, GI31543397, Length=422, Percent_Identity=46.4454976303318, Blast_Score=330, Evalue=1e-90,
Organism=Escherichia coli, GI1789294, Length=402, Percent_Identity=43.2835820895522, Blast_Score=299, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI17508823, Length=419, Percent_Identity=47.255369928401, Blast_Score=343, Evalue=8e-95,
Organism=Saccharomyces cerevisiae, GI6319857, Length=427, Percent_Identity=47.7751756440281, Blast_Score=348, Evalue=8e-97,
Organism=Drosophila melanogaster, GI17136394, Length=422, Percent_Identity=45.260663507109, Blast_Score=314, Evalue=6e-86,
Organism=Drosophila melanogaster, GI24581203, Length=426, Percent_Identity=42.9577464788732, Blast_Score=310, Evalue=2e-84,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 43348; Mature: 43217

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLNKKSIDDINVKGKRVLVRCDFNVPLKDGKITDENRINGALPTIQKLIKDGGKVILCS
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEE
HLGKVKNGPNEGESLAPVAAALEAKLGQKVTFVADYNVTGEAATKAVSEMKEGDVVLLQN
HHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEC
TRFRGKEETKPQEAGDAFAKELADLADVYVCDAFGSAHRAHASVAGVTKFITAKGGENVV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEECCCCCHHH
GYLMQKEIDFLGKAVENPVRPFVAILGGAKVADKLNVIDNLLEKADTLIIGGGMAYTFLK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCEEEECCCCEEEEEE
AQGYEIGISMLDETKIDYCKEMLAKAEKLGKKILLPVDAVTIKDFPNPIDAPVETETYDY
CCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEEECCCCCCCCCCCCCCCCCC
DKMPADREGCDIGPKTRELFADAVASAKTVVWNGPMGVFENPTLAAGTLAVAEALAKSDA
CCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCC
ITIIGGGDSAAAVNQMGLGDKMSHISTGGGASLEFLEGKELPGVACADDK
EEEEECCCCHHHHHHCCCCCHHHHCCCCCCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
SLNKKSIDDINVKGKRVLVRCDFNVPLKDGKITDENRINGALPTIQKLIKDGGKVILCS
CCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEE
HLGKVKNGPNEGESLAPVAAALEAKLGQKVTFVADYNVTGEAATKAVSEMKEGDVVLLQN
HHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEC
TRFRGKEETKPQEAGDAFAKELADLADVYVCDAFGSAHRAHASVAGVTKFITAKGGENVV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEECCCCCHHH
GYLMQKEIDFLGKAVENPVRPFVAILGGAKVADKLNVIDNLLEKADTLIIGGGMAYTFLK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCEEEECCCCEEEEEE
AQGYEIGISMLDETKIDYCKEMLAKAEKLGKKILLPVDAVTIKDFPNPIDAPVETETYDY
CCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEEECCCCCCCCCCCCCCCCCC
DKMPADREGCDIGPKTRELFADAVASAKTVVWNGPMGVFENPTLAAGTLAVAEALAKSDA
CCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCC
ITIIGGGDSAAAVNQMGLGDKMSHISTGGGASLEFLEGKELPGVACADDK
EEEEECCCCHHHHHHCCCCCHHHHCCCCCCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10463150; 11466286 [H]