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The map label for this gene is hisF [H]

Identifier: 238923840

GI number: 238923840

Start: 1346454

End: 1347215

Strand: Direct

Name: hisF [H]

Synonym: EUBREC_1468

Alternate gene names: 238923840

Gene position: 1346454-1347215 (Clockwise)

Preceding gene: 238923839

Following gene: 238923841

Centisome position: 39.03

GC content: 47.38

Gene sequence:

>762_bases
ATGTTTACGAAGAGAATAATCCCATGTCTGGATGTGAATAACGGACGTGTTGTTAAGGGCATAAACTTTGTCAATTTAAG
AGATGCAGGTGACCCGGTTGAGATTGCGGCTGCTTATGATAAGGCAGGTGCAGATGAGGTGGTTTTTCTGGATATTACGG
CGTCAAGCGACAACAGAAATACTGTTGTGGATATGGTCAGAAAGGTTGCTGAAAAGGTGTTTATACCGTTCACTGTGGGC
GGCGGCATCAGAACAGTCGATGATTTTAAGGCACTTCTTAGAGAGGGTGCGGACAAGATTTCAATAAATTCAAGTGCCAT
AAACACACCGCGGCTTATCAGCGACGCTGCGGATAAGTTTGGCAGCCAGTGTGTGGTTGTGGCAATCGATGCAAGGCGTC
GTGTTGACGGAAGCGGCTGGAATGTATACAAGAACGGAGGCCGCATAGATACAGGCCTTGATGCCATTGAATGGGCTGTT
AAGGCAAATGAGCTTGGAGCGGGAGAGATACTTCTTACAAGCATGGACTGTGACGGCACAAAGGATGGCTACGATATCGA
GCTTACAAGGTTTATTGCCGATAACGTTTCTGTTCCGGTTATCGCATCGGGCGGTGCAGGCACAAAGGAGCATTTTTACG
AGGCTCTGACCGAGGGAAAGGCGGACGCAGCGCTTGCAGCTTCACTTTTCCACTACAAGGAGCTTGAAATTATGGATTTA
AAGAATTATCTGGCAGATAAAGGTGTGTCGGTCAGAAGATAG

Upstream 100 bases:

>100_bases
GAGAAAAACAATGTGTTCGCCTGCCAGTTCCATCCGGAAAAGAGCAGCACAGTCGGACTTTCGATACTTAAAAATTTTGC
AGGGATAGGGGAGGCATAAT

Downstream 100 bases:

>100_bases
GCGGGAGATAAAGATTATGAGTATGCTTGATGGCGAGTGGGCAAAGGCTGTGGGAGCCGAGTTTAAAAAACCGTATTATA
GGGATTTGTACAATTTTGTA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MFTKRIIPCLDVNNGRVVKGINFVNLRDAGDPVEIAAAYDKAGADEVVFLDITASSDNRNTVVDMVRKVAEKVFIPFTVG
GGIRTVDDFKALLREGADKISINSSAINTPRLISDAADKFGSQCVVVAIDARRRVDGSGWNVYKNGGRIDTGLDAIEWAV
KANELGAGEILLTSMDCDGTKDGYDIELTRFIADNVSVPVIASGGAGTKEHFYEALTEGKADAALAASLFHYKELEIMDL
KNYLADKGVSVRR

Sequences:

>Translated_253_residues
MFTKRIIPCLDVNNGRVVKGINFVNLRDAGDPVEIAAAYDKAGADEVVFLDITASSDNRNTVVDMVRKVAEKVFIPFTVG
GGIRTVDDFKALLREGADKISINSSAINTPRLISDAADKFGSQCVVVAIDARRRVDGSGWNVYKNGGRIDTGLDAIEWAV
KANELGAGEILLTSMDCDGTKDGYDIELTRFIADNVSVPVIASGGAGTKEHFYEALTEGKADAALAASLFHYKELEIMDL
KNYLADKGVSVRR
>Mature_253_residues
MFTKRIIPCLDVNNGRVVKGINFVNLRDAGDPVEIAAAYDKAGADEVVFLDITASSDNRNTVVDMVRKVAEKVFIPFTVG
GGIRTVDDFKALLREGADKISINSSAINTPRLISDAADKFGSQCVVVAIDARRRVDGSGWNVYKNGGRIDTGLDAIEWAV
KANELGAGEILLTSMDCDGTKDGYDIELTRFIADNVSVPVIASGGAGTKEHFYEALTEGKADAALAASLFHYKELEIMDL
KNYLADKGVSVRR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=47.4708171206226, Blast_Score=226, Evalue=1e-60,
Organism=Escherichia coli, GI87082028, Length=213, Percent_Identity=28.6384976525822, Blast_Score=78, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6319725, Length=313, Percent_Identity=34.185303514377, Blast_Score=160, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27267; Mature: 27267

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTKRIIPCLDVNNGRVVKGINFVNLRDAGDPVEIAAAYDKAGADEVVFLDITASSDNRN
CCCCCCCCEEECCCCEEEECEEEEEECCCCCCEEEEEECCCCCCCCEEEEEEEECCCCCH
TVVDMVRKVAEKVFIPFTVGGGIRTVDDFKALLREGADKISINSSAINTPRLISDAADKF
HHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHH
GSQCVVVAIDARRRVDGSGWNVYKNGGRIDTGLDAIEWAVKANELGAGEILLTSMDCDGT
CCCEEEEEEECCCCCCCCCCEEEECCCEECCCHHHHHHEEECCCCCCCEEEEEEECCCCC
KDGYDIELTRFIADNVSVPVIASGGAGTKEHFYEALTEGKADAALAASLFHYKELEIMDL
CCCCEEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEHH
KNYLADKGVSVRR
HHHHHHCCCCCCC
>Mature Secondary Structure
MFTKRIIPCLDVNNGRVVKGINFVNLRDAGDPVEIAAAYDKAGADEVVFLDITASSDNRN
CCCCCCCCEEECCCCEEEECEEEEEECCCCCCEEEEEECCCCCCCCEEEEEEEECCCCCH
TVVDMVRKVAEKVFIPFTVGGGIRTVDDFKALLREGADKISINSSAINTPRLISDAADKF
HHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHH
GSQCVVVAIDARRRVDGSGWNVYKNGGRIDTGLDAIEWAVKANELGAGEILLTSMDCDGT
CCCEEEEEEECCCCCCCCCCEEEECCCEECCCHHHHHHEEECCCCCCCEEEEEEECCCCC
KDGYDIELTRFIADNVSVPVIASGGAGTKEHFYEALTEGKADAALAASLFHYKELEIMDL
CCCCEEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEHH
KNYLADKGVSVRR
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA