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The map label for this gene is tilS [H]

Identifier: 238922945

GI number: 238922945

Start: 491620

End: 493164

Strand: Direct

Name: tilS [H]

Synonym: EUBREC_0533

Alternate gene names: 238922945

Gene position: 491620-493164 (Clockwise)

Preceding gene: 238922944

Following gene: 238922946

Centisome position: 14.25

GC content: 47.18

Gene sequence:

>1545_bases
ATGATTAGACGGACAAAAGATTATATTCTGGAAAACAAGATGATAAACAATCACAGCACGGTGCTTGTTGCGCTGTCGGG
CGGTGCGGATTCGGTCTGCCTTCTGCTGGTGTTAAATGAGATAAAAAGACAGTGCGCAGATGAGCTTGATTTTGCGCTGG
AGGCAGTACATGTGGAGCATGGCATCAGAGGAGCCGAGAGCAGGGAGGATGCGAGGTTTGCGTCAGATATCTGCGAGAGG
CTTAACATACCGTGCCATGTGCATTCTGTGGATGTACCGCAATATGCAGCAAGTCATGGACTCGGACTGGAGGAGGCCGC
CAGGATATTGCGCTATGAAGCGTTTGAAAAGGAAGTCGCCAGGATATTGCGCTATGAGGCGTTTGAAGAGGGGGCAGGCA
GATATCCCTGTGAACCGGCCAATGCATCAGACCAAAAACAGGGTAACAGCAGGCAGGTAGTCGTTGCAGTTGCGCACCAT
ATGGAGGACAACGCAGAGACAGTTCTTTTTCAGATGCTTCGCGGAAGCGGAGCGAAGGGGCTATCTGGCATGCATCCGGT
TTCAGTGAAGAACGGTGTCACCTATATCAGGCCGCTTTTATCAGCAACACGCGCACAGATAGAGGAATATCTGAAGGAAA
ACGGACAGGCTTTTGTCACCGATGCCACAAACACTGACACAGCCTACTCCAGAAACAAGCTGCGCCATGATGTGTTTCCT
TTGCTTTTGCAGATCAATGACAGGGCGATAGAGCATATCAATGAGAGCGCAGGACAGCTTGCGGTGATGAATGATTTTTA
CGAGCAGCAGCTAAAAGAGGCGTGCGACAGCATGGTTTCAAAAAAAGAAGGCAGGGTGATACTTGAAATCAGCCGTTTTG
AGTGTCTGCATCCGGCATTAAAAAGCGGAGTGGCGCGCGAGTGTATACATCTGGCGTCAGGGCGGCTCAAGGACATCACG
AGCACGCATATCGGTGCCCTTGTGAAGCTTGCAGGGCAGCAGTCGGGCAGAAAAATCAATCTGCCGTATGGTATTATTGC
GGAAAAATCATTTGGCGAGGTGATACTCTTCGCCGAAAGGTGTGACGATGAAAAAGAAGAAATTCACATTACGCAAAAAG
AGCTTGAGGTACTGAGTGACACGGGAGAGCAAAAAAACATAAAGCTTTCGGCAGATGGCTCATATGTCACACTTTGTATA
CAAGATTTTAATGGTAAAATGGACGAAATTCCCAAAAAACCATATACGAAATGGTTTGATTATGATAAGATGAAGAAAGG
CTTTGAAATAAGAACAAGAAAAGCCGGTGACTATATAATTGTGGATGACGAGGGCCATCATAAGAAGCTGAAGCAGGTAT
TTACAGGTGATAAAATTCCTGCACAGCAGAGAGCGGGGCTGTGGCTCATAGCCCATGAATCACTGGTGCATGCCATTATC
GGATACAGATCCGGCTGCAGCGCACTTGTCACACCACAGACAGGAAAAGTACTTAAGATCACATTTTACGGAGGAAAACA
AAATGGATTTTTCAAAAAAATATGA

Upstream 100 bases:

>100_bases
GCGTGATGGCGCAGGAAATTCTGGACAGGGTGCTGCTTGACACAGGTGGATACGCCATGGATGATATGACTGTAGTTGCA
ATAGGAATCTGGGAAAAATA

Downstream 100 bases:

>100_bases
TATTTCGGTTTACCTGACAGAGGAGCAGCTCAATAAGCGTATTGCAGAGATTGGAGCGCAAATCACAGAAAAATACAGGG
GACAGTCAGTATATCTCATT

Product: cell cycle protein MesJ, putative

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MIRRTKDYILENKMINNHSTVLVALSGGADSVCLLLVLNEIKRQCADELDFALEAVHVEHGIRGAESREDARFASDICER
LNIPCHVHSVDVPQYAASHGLGLEEAARILRYEAFEKEVARILRYEAFEEGAGRYPCEPANASDQKQGNSRQVVVAVAHH
MEDNAETVLFQMLRGSGAKGLSGMHPVSVKNGVTYIRPLLSATRAQIEEYLKENGQAFVTDATNTDTAYSRNKLRHDVFP
LLLQINDRAIEHINESAGQLAVMNDFYEQQLKEACDSMVSKKEGRVILEISRFECLHPALKSGVARECIHLASGRLKDIT
STHIGALVKLAGQQSGRKINLPYGIIAEKSFGEVILFAERCDDEKEEIHITQKELEVLSDTGEQKNIKLSADGSYVTLCI
QDFNGKMDEIPKKPYTKWFDYDKMKKGFEIRTRKAGDYIIVDDEGHHKKLKQVFTGDKIPAQQRAGLWLIAHESLVHAII
GYRSGCSALVTPQTGKVLKITFYGGKQNGFFKKI

Sequences:

>Translated_514_residues
MIRRTKDYILENKMINNHSTVLVALSGGADSVCLLLVLNEIKRQCADELDFALEAVHVEHGIRGAESREDARFASDICER
LNIPCHVHSVDVPQYAASHGLGLEEAARILRYEAFEKEVARILRYEAFEEGAGRYPCEPANASDQKQGNSRQVVVAVAHH
MEDNAETVLFQMLRGSGAKGLSGMHPVSVKNGVTYIRPLLSATRAQIEEYLKENGQAFVTDATNTDTAYSRNKLRHDVFP
LLLQINDRAIEHINESAGQLAVMNDFYEQQLKEACDSMVSKKEGRVILEISRFECLHPALKSGVARECIHLASGRLKDIT
STHIGALVKLAGQQSGRKINLPYGIIAEKSFGEVILFAERCDDEKEEIHITQKELEVLSDTGEQKNIKLSADGSYVTLCI
QDFNGKMDEIPKKPYTKWFDYDKMKKGFEIRTRKAGDYIIVDDEGHHKKLKQVFTGDKIPAQQRAGLWLIAHESLVHAII
GYRSGCSALVTPQTGKVLKITFYGGKQNGFFKKI
>Mature_514_residues
MIRRTKDYILENKMINNHSTVLVALSGGADSVCLLLVLNEIKRQCADELDFALEAVHVEHGIRGAESREDARFASDICER
LNIPCHVHSVDVPQYAASHGLGLEEAARILRYEAFEKEVARILRYEAFEEGAGRYPCEPANASDQKQGNSRQVVVAVAHH
MEDNAETVLFQMLRGSGAKGLSGMHPVSVKNGVTYIRPLLSATRAQIEEYLKENGQAFVTDATNTDTAYSRNKLRHDVFP
LLLQINDRAIEHINESAGQLAVMNDFYEQQLKEACDSMVSKKEGRVILEISRFECLHPALKSGVARECIHLASGRLKDIT
STHIGALVKLAGQQSGRKINLPYGIIAEKSFGEVILFAERCDDEKEEIHITQKELEVLSDTGEQKNIKLSADGSYVTLCI
QDFNGKMDEIPKKPYTKWFDYDKMKKGFEIRTRKAGDYIIVDDEGHHKKLKQVFTGDKIPAQQRAGLWLIAHESLVHAII
GYRSGCSALVTPQTGKVLKITFYGGKQNGFFKKI

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=233, Percent_Identity=33.0472103004292, Blast_Score=111, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 57460; Mature: 57460

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRRTKDYILENKMINNHSTVLVALSGGADSVCLLLVLNEIKRQCADELDFALEAVHVEH
CCCCCHHHHHHCCEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIRGAESREDARFASDICERLNIPCHVHSVDVPQYAASHGLGLEEAARILRYEAFEKEVA
CCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHH
RILRYEAFEEGAGRYPCEPANASDQKQGNSRQVVVAVAHHMEDNAETVLFQMLRGSGAKG
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHCCCCHHHHHHHHHHCCCCCC
LSGMHPVSVKNGVTYIRPLLSATRAQIEEYLKENGQAFVTDATNTDTAYSRNKLRHDVFP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHH
LLLQINDRAIEHINESAGQLAVMNDFYEQQLKEACDSMVSKKEGRVILEISRFECLHPAL
HHHHCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHH
KSGVARECIHLASGRLKDITSTHIGALVKLAGQQSGRKINLPYGIIAEKSFGEVILFAER
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEECCCCCCEEEEHHH
CDDEKEEIHITQKELEVLSDTGEQKNIKLSADGSYVTLCIQDFNGKMDEIPKKPYTKWFD
CCCCHHHEEHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEECCCCCHHHCCCCCCHHHCC
YDKMKKGFEIRTRKAGDYIIVDDEGHHKKLKQVFTGDKIPAQQRAGLWLIAHESLVHAII
HHHHHCCCEEEEECCCCEEEECCCCHHHHHHHHHCCCCCCHHHHCCEEEEEHHHHHHHHH
GYRSGCSALVTPQTGKVLKITFYGGKQNGFFKKI
HHCCCCCEEECCCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MIRRTKDYILENKMINNHSTVLVALSGGADSVCLLLVLNEIKRQCADELDFALEAVHVEH
CCCCCHHHHHHCCEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIRGAESREDARFASDICERLNIPCHVHSVDVPQYAASHGLGLEEAARILRYEAFEKEVA
CCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHH
RILRYEAFEEGAGRYPCEPANASDQKQGNSRQVVVAVAHHMEDNAETVLFQMLRGSGAKG
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHCCCCHHHHHHHHHHCCCCCC
LSGMHPVSVKNGVTYIRPLLSATRAQIEEYLKENGQAFVTDATNTDTAYSRNKLRHDVFP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHH
LLLQINDRAIEHINESAGQLAVMNDFYEQQLKEACDSMVSKKEGRVILEISRFECLHPAL
HHHHCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHH
KSGVARECIHLASGRLKDITSTHIGALVKLAGQQSGRKINLPYGIIAEKSFGEVILFAER
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEECCCCCCEEEEHHH
CDDEKEEIHITQKELEVLSDTGEQKNIKLSADGSYVTLCIQDFNGKMDEIPKKPYTKWFD
CCCCHHHEEHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEECCCCCHHHCCCCCCHHHCC
YDKMKKGFEIRTRKAGDYIIVDDEGHHKKLKQVFTGDKIPAQQRAGLWLIAHESLVHAII
HHHHHCCCEEEEECCCCEEEECCCCHHHHHHHHHCCCCCCHHHHCCEEEEEHHHHHHHHH
GYRSGCSALVTPQTGKVLKITFYGGKQNGFFKKI
HHCCCCCEEECCCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA