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The map label for this gene is spoIIE [H]

Identifier: 238922944

GI number: 238922944

Start: 489755

End: 491620

Strand: Direct

Name: spoIIE [H]

Synonym: EUBREC_0532

Alternate gene names: 238922944

Gene position: 489755-491620 (Clockwise)

Preceding gene: 238922943

Following gene: 238922945

Centisome position: 14.2

GC content: 46.89

Gene sequence:

>1866_bases
TTGAAAAAAGATGGAGGCAAAAAGATGAGGATTGCAAATTTAAAAAAGGCTGTGTGGGGGCTTATGGCATTTGCATCCAC
ACTGGTGTGTGTGATGGATTGTTATCCGCTGATACCTGCAGTGTATGGAGTTTATTGTCTGAGCAGTGGGCATACGATTA
TTTTTTACATAGGATTAATTATTGGAATGGGATACTTTATATCGATACCGTCGATATGTAAGTATCTCTTTATTATTGCG
GTGATTTACTTTGGGGAAAGGCTATTTGTGAGAAAAAGCAGTAAAAACGGATGTCTGACAACAGCCGTTGTAGCAGCTTG
TGCCACGGCGGTGATGAATCTGTCGGTAACATTTCTGGGAAAGCCGTATACGGATGAGATAGTGTTGTCGGTAGCAGAGA
GCCTTGTTGTGTTTTCGATGGCCTTTGTGCTTTGCAGAGCATGCGAATATTTAAGGGCTCTTGAGCACAATGAAAACCCG
GTCATAGCAGGACTCCTGCCCGACAGGGAGGAGGCATTTGCCACAGCAGTTTCAGGACTTTCAGGCATTATTTCGACTGC
AAACGTCATGGCTGTAAAATGTACGGCAGATAAAATCCCTGATGAGAGTGAGAAAATACAGCTTGAGGTGACAGGGAGAC
TTTGTGCATGTTGTGAGGGCTGCAGTGTGTGCTGGACAAGCGGGACTTCGATTTCCGACAGTATAAAAATGCTTGCTGAT
GCAGTGCGCAAACGTATGAAAACAGAGGAAATTGTGCAAAACAGATATGTGGATGGCTGCCCGCACTATACGCGCATGGT
GGAAGCCGCCACAGAGGCATTTGCCCGTATTGAGCTAAATGAAGCCTGGTACAGGCGTCTCACGGAAAACAGACGCGTCA
TAGCCACACAGCTTGACGCTATGGCAGAGCTCATGGATAGCTGGTGCAGGGCTGAAAAATGTATTGATAAAAAAAGAAGG
CTGCGTCTTACAAGAGTTTATGTATATACAAAAGAGGCTGGTATACAGGTGGAAAATGCCCATATTTATGAGAACGCAAG
GCAGCAGGTATGTATAAAGGCAGATGTCTGCACCAAGATAGATGGCGGGATTGAAATTTCAAAGTATGTGCAGGCGGTTT
CACGCGCCATGGGAGTGAAGCTCAGGCAGGCACATGGGACAGTTTCAATAATATCCGATGAGAGGACATCTATTGTGCTT
TACGAGGAGAATCAGTTCTATGCACTTTCGGGGGTGGCGACCAAAAAGAAGACCGGCTCTCAGGCAAATGGCGACAGCTG
CAGCATGTTTCAGCTGGATGATGGCATGTATCATGTATGTGTATCCGATGGCATGGGCTCGGGCAAGCAGGCGCAGGCCG
AGAGCACACTGGTGGTGGATTTGCTTGAAAAGCTTTTGGAGGCCGGCTTTAGCAGGGAAAGTGCCCTAAAGCTGATGAAC
TCTGCCATGGTCATATCGGCGGGCGAGGAGTCCTATTCCACGGTTGATTTTGCCACAATAGACATGTACACAGGTGAGCT
TGAGCTGGCCAAAACAGGCGCCGCACCGTCATTTATCAAATCAGGGAAACAAGTAAGTGTTATAGAAAATGAATCACTCC
CGGCGGGGGTCGATGTCTGGCAGGAGAGCAAACATTCAAAAAATACACTTCAAAGTGGCGATTTCCTTGTCATGGTGACG
GATGGTGTGCTAGAATATCTGCATGTAAAGGACAGACAGGGGAAACTTATGGATATCATCGCAGGCGTCAAAAGCGACAA
TGCAGGCGTGATGGCGCAGGAAATTCTGGACAGGGTGCTGCTTGACACAGGTGGATACGCCATGGATGATATGACTGTAG
TTGCAATAGGAATCTGGGAAAAATAA

Upstream 100 bases:

>100_bases
GTATGACAATGAAATAATCTTCAAGGAAAAGGACAAATAATTTTTGAAGATATAGTTGCGCTTTTTCGTCAAAACCTTCA
TTTTTGACCACATATAATCG

Downstream 100 bases:

>100_bases
TGATTAGACGGACAAAAGATTATATTCTGGAAAACAAGATGATAAACAATCACAGCACGGTGCTTGTTGCGCTGTCGGGC
GGTGCGGATTCGGTCTGCCT

Product: phosphoprotein phosphatase

Products: NA

Alternate protein names: Stage II sporulation protein H [H]

Number of amino acids: Translated: 621; Mature: 621

Protein sequence:

>621_residues
MKKDGGKKMRIANLKKAVWGLMAFASTLVCVMDCYPLIPAVYGVYCLSSGHTIIFYIGLIIGMGYFISIPSICKYLFIIA
VIYFGERLFVRKSSKNGCLTTAVVAACATAVMNLSVTFLGKPYTDEIVLSVAESLVVFSMAFVLCRACEYLRALEHNENP
VIAGLLPDREEAFATAVSGLSGIISTANVMAVKCTADKIPDESEKIQLEVTGRLCACCEGCSVCWTSGTSISDSIKMLAD
AVRKRMKTEEIVQNRYVDGCPHYTRMVEAATEAFARIELNEAWYRRLTENRRVIATQLDAMAELMDSWCRAEKCIDKKRR
LRLTRVYVYTKEAGIQVENAHIYENARQQVCIKADVCTKIDGGIEISKYVQAVSRAMGVKLRQAHGTVSIISDERTSIVL
YEENQFYALSGVATKKKTGSQANGDSCSMFQLDDGMYHVCVSDGMGSGKQAQAESTLVVDLLEKLLEAGFSRESALKLMN
SAMVISAGEESYSTVDFATIDMYTGELELAKTGAAPSFIKSGKQVSVIENESLPAGVDVWQESKHSKNTLQSGDFLVMVT
DGVLEYLHVKDRQGKLMDIIAGVKSDNAGVMAQEILDRVLLDTGGYAMDDMTVVAIGIWEK

Sequences:

>Translated_621_residues
MKKDGGKKMRIANLKKAVWGLMAFASTLVCVMDCYPLIPAVYGVYCLSSGHTIIFYIGLIIGMGYFISIPSICKYLFIIA
VIYFGERLFVRKSSKNGCLTTAVVAACATAVMNLSVTFLGKPYTDEIVLSVAESLVVFSMAFVLCRACEYLRALEHNENP
VIAGLLPDREEAFATAVSGLSGIISTANVMAVKCTADKIPDESEKIQLEVTGRLCACCEGCSVCWTSGTSISDSIKMLAD
AVRKRMKTEEIVQNRYVDGCPHYTRMVEAATEAFARIELNEAWYRRLTENRRVIATQLDAMAELMDSWCRAEKCIDKKRR
LRLTRVYVYTKEAGIQVENAHIYENARQQVCIKADVCTKIDGGIEISKYVQAVSRAMGVKLRQAHGTVSIISDERTSIVL
YEENQFYALSGVATKKKTGSQANGDSCSMFQLDDGMYHVCVSDGMGSGKQAQAESTLVVDLLEKLLEAGFSRESALKLMN
SAMVISAGEESYSTVDFATIDMYTGELELAKTGAAPSFIKSGKQVSVIENESLPAGVDVWQESKHSKNTLQSGDFLVMVT
DGVLEYLHVKDRQGKLMDIIAGVKSDNAGVMAQEILDRVLLDTGGYAMDDMTVVAIGIWEK
>Mature_621_residues
MKKDGGKKMRIANLKKAVWGLMAFASTLVCVMDCYPLIPAVYGVYCLSSGHTIIFYIGLIIGMGYFISIPSICKYLFIIA
VIYFGERLFVRKSSKNGCLTTAVVAACATAVMNLSVTFLGKPYTDEIVLSVAESLVVFSMAFVLCRACEYLRALEHNENP
VIAGLLPDREEAFATAVSGLSGIISTANVMAVKCTADKIPDESEKIQLEVTGRLCACCEGCSVCWTSGTSISDSIKMLAD
AVRKRMKTEEIVQNRYVDGCPHYTRMVEAATEAFARIELNEAWYRRLTENRRVIATQLDAMAELMDSWCRAEKCIDKKRR
LRLTRVYVYTKEAGIQVENAHIYENARQQVCIKADVCTKIDGGIEISKYVQAVSRAMGVKLRQAHGTVSIISDERTSIVL
YEENQFYALSGVATKKKTGSQANGDSCSMFQLDDGMYHVCVSDGMGSGKQAQAESTLVVDLLEKLLEAGFSRESALKLMN
SAMVISAGEESYSTVDFATIDMYTGELELAKTGAAPSFIKSGKQVSVIENESLPAGVDVWQESKHSKNTLQSGDFLVMVT
DGVLEYLHVKDRQGKLMDIIAGVKSDNAGVMAQEILDRVLLDTGGYAMDDMTVVAIGIWEK

Specific function: Normally needed for pro-sigma E processing during sporulation but can be bypassed in vegetative cells. Activates spoIIAA by dephosphorylation [H]

COG id: COG2208

COG function: function code TK; Serine phosphatase RsbU, regulator of sigma subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein. Note=Polar septum [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PP2C-like domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001932
- InterPro:   IPR014221
- InterPro:   IPR010822 [H]

Pfam domain/function: PF07228 SpoIIE [H]

EC number: =3.1.3.16 [H]

Molecular weight: Translated: 68109; Mature: 68109

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS00079 MULTICOPPER_OXIDASE1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKDGGKKMRIANLKKAVWGLMAFASTLVCVMDCYPLIPAVYGVYCLSSGHTIIFYIGLI
CCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
IGMGYFISIPSICKYLFIIAVIYFGERLFVRKSSKNGCLTTAVVAACATAVMNLSVTFLG
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHCEEEECC
KPYTDEIVLSVAESLVVFSMAFVLCRACEYLRALEHNENPVIAGLLPDREEAFATAVSGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHH
SGIISTANVMAVKCTADKIPDESEKIQLEVTGRLCACCEGCSVCWTSGTSISDSIKMLAD
HHHHHCCCEEEEEEECCCCCCCCCEEEEEECCHHHHHHCCCCEEEECCCCHHHHHHHHHH
AVRKRMKTEEIVQNRYVDGCPHYTRMVEAATEAFARIELNEAWYRRLTENRRVIATQLDA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEECHHHHHHHHCCCEEEHHHHHH
MAELMDSWCRAEKCIDKKRRLRLTRVYVYTKEAGIQVENAHIYENARQQVCIKADVCTKI
HHHHHHHHHHHHHHHHHHHCEEEEEEEEEEECCCCEEECCHHHHHHHHCEEEEEHHHHCC
DGGIEISKYVQAVSRAMGVKLRQAHGTVSIISDERTSIVLYEENQFYALSGVATKKKTGS
CCCCCHHHHHHHHHHHHCCEEEECCCEEEEEECCCEEEEEEECCCEEEEECCCCCCCCCC
QANGDSCSMFQLDDGMYHVCVSDGMGSGKQAQAESTLVVDLLEKLLEAGFSRESALKLMN
CCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
SAMVISAGEESYSTVDFATIDMYTGELELAKTGAAPSFIKSGKQVSVIENESLPAGVDVW
CEEEEECCCCCCCEEEEEEEEEEECCEEEECCCCCHHHHHCCCEEEEEECCCCCCCCHHH
QESKHSKNTLQSGDFLVMVTDGVLEYLHVKDRQGKLMDIIAGVKSDNAGVMAQEILDRVL
HHCCCCCCCCCCCCEEEEEEHHHHHHHHCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHH
LDTGGYAMDDMTVVAIGIWEK
HCCCCCEECCEEEEEEEECCC
>Mature Secondary Structure
MKKDGGKKMRIANLKKAVWGLMAFASTLVCVMDCYPLIPAVYGVYCLSSGHTIIFYIGLI
CCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
IGMGYFISIPSICKYLFIIAVIYFGERLFVRKSSKNGCLTTAVVAACATAVMNLSVTFLG
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHCEEEECC
KPYTDEIVLSVAESLVVFSMAFVLCRACEYLRALEHNENPVIAGLLPDREEAFATAVSGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHH
SGIISTANVMAVKCTADKIPDESEKIQLEVTGRLCACCEGCSVCWTSGTSISDSIKMLAD
HHHHHCCCEEEEEEECCCCCCCCCEEEEEECCHHHHHHCCCCEEEECCCCHHHHHHHHHH
AVRKRMKTEEIVQNRYVDGCPHYTRMVEAATEAFARIELNEAWYRRLTENRRVIATQLDA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEECHHHHHHHHCCCEEEHHHHHH
MAELMDSWCRAEKCIDKKRRLRLTRVYVYTKEAGIQVENAHIYENARQQVCIKADVCTKI
HHHHHHHHHHHHHHHHHHHCEEEEEEEEEEECCCCEEECCHHHHHHHHCEEEEEHHHHCC
DGGIEISKYVQAVSRAMGVKLRQAHGTVSIISDERTSIVLYEENQFYALSGVATKKKTGS
CCCCCHHHHHHHHHHHHCCEEEECCCEEEEEECCCEEEEEEECCCEEEEECCCCCCCCCC
QANGDSCSMFQLDDGMYHVCVSDGMGSGKQAQAESTLVVDLLEKLLEAGFSRESALKLMN
CCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
SAMVISAGEESYSTVDFATIDMYTGELELAKTGAAPSFIKSGKQVSVIENESLPAGVDVW
CEEEEECCCCCCCEEEEEEEEEEECCEEEECCCCCHHHHHCCCEEEEEECCCCCCCCHHH
QESKHSKNTLQSGDFLVMVTDGVLEYLHVKDRQGKLMDIIAGVKSDNAGVMAQEILDRVL
HHCCCCCCCCCCCCEEEEEEHHHHHHHHCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHH
LDTGGYAMDDMTVVAIGIWEK
HCCCCCEECCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7584024; 8830262; 9384377; 2832371; 8113187; 7570023 [H]