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The map label for this gene is thiH [H]

Identifier: 238922660

GI number: 238922660

Start: 216620

End: 218047

Strand: Direct

Name: thiH [H]

Synonym: EUBREC_0235

Alternate gene names: 238922660

Gene position: 216620-218047 (Clockwise)

Preceding gene: 238922659

Following gene: 238922661

Centisome position: 6.28

GC content: 45.52

Gene sequence:

>1428_bases
ATGTATAACGTAAAATCATTAAAGGCTGAAGAATTTATTTCAGACGAGGAAATCAAAGAGACTCTCGAATACGCAGAGGC
AAACAAAAATAATATAGGGCTTATCGATGCGATTATCGAGAAAGCAAAGCTAAAAAAAGGACTCACACACAGAGAGGCAT
CAGTGCTTTTAGCCTGTGAGATTCCGGAGAAGCTTGACGAGGTTTACAAGCTTGCACAGCAGATAAAGAAGGATTTCTAC
GGAAACAGAATCGTGCTTTTTGCACCGCTTTATCTGTCAAACTATTGTGTGAACGGCTGTGTTTACTGTCCATATCACAT
GAAAAATAAGCATATTGCGAGAAAGAAGCTCACTCAGGAGGAGATAGTAAAGGAGGTCACAGCCCTTCAGGATATGGGAC
ACAAGCGTCTTGCAATCGAGGCAGGAGAGGACCCTGTAAACAATCCGATTGAGTATATTCTCGAGTGTATCAACACAATC
TACAGCATCAAGCACAAAAACGGAGCTATCCGCCGTGTAAATGTAAATATCGCTGCCACTACAGTGGAGAATTACAGAAA
GCTCAAGGAGGCAGGAATCGGAACCTACATACTCTTCCAGGAGACATATCACAAGGAGAGCTATGAGCAGCTTCATCCGA
CAGGACCAAAACATGACTATGCATATCACACAGAGGCAATGGACCGTGCTATGGAGGGCGGCATTGACGATGTGGGACTT
GGTGTACTTTTCGGACTGGACAAATACAAGTACGAGTTTGCAGGACTTTTGATGCATGCAGAGCACCTTGAGGCAGTGCA
CGGTGTAGGTCCTCACACCATCAGTGTGCCGCGTATAAAGCACGCAGATGATATAGACCCGGATGTATTTGACAATGGCA
TCAGTGATGATACCTTCGCCAAAATATGCGCACTTATCCGTATTTCAGTGCCATACACAGGAATGATAATCTCAACCCGT
GAGAGCCAGACTGTCCGTGAAAAGGTTCTGCCGCTCGGTGTTTCACAGATCAGCGGCGCATCAAAGACAAGTGTTGGAGG
CTATGCAGACCCGGAGGCTGAGAAAAATGCAGAGGCAACATCGGAGCAGTTTGATGTCAGCGACCAGAGAACACTTGACG
AGGTGGTAAACTGGCTTATGAAGATGGACTATATTCCGTCCTTCTGTACAGCCTGCTACAGAGAAGGCAGAACGGGAGAC
CGTTTTATGGCACTGTGCAAGAGCATGCAGATACTTAATTGCTGTCATCCGAACGCACTCATGACACTTAAGGAGTATCT
CGAGGACTATGCAAGCCCGCAGACAAGAGAGCTTGGAATGAAGCTCATCGATAGGGAAATAGAAAAGATTACCAATCCAA
AGGTTAAGCAGACCTGCTATGAGCATATACATGATATTGCAGACGGAAAGCGTGATTTCCGTTTCTAA

Upstream 100 bases:

>100_bases
GCTATCAGGTGGTGGTGTCGCGTGGGGACTACGCAAATTTTTGATTAATAGTTACGATTAATAAATATAAAACAACACCA
GGATATAGAAGGAGATAAAA

Downstream 100 bases:

>100_bases
AGGAGGAAATATGGGAAATTTGAATGAGACGCCTTCTGCCAACAGACTTCATATAGGTATATACGGAAAGACAAACAGTG
GTAAGTCATCTCTGATAAAT

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MYNVKSLKAEEFISDEEIKETLEYAEANKNNIGLIDAIIEKAKLKKGLTHREASVLLACEIPEKLDEVYKLAQQIKKDFY
GNRIVLFAPLYLSNYCVNGCVYCPYHMKNKHIARKKLTQEEIVKEVTALQDMGHKRLAIEAGEDPVNNPIEYILECINTI
YSIKHKNGAIRRVNVNIAATTVENYRKLKEAGIGTYILFQETYHKESYEQLHPTGPKHDYAYHTEAMDRAMEGGIDDVGL
GVLFGLDKYKYEFAGLLMHAEHLEAVHGVGPHTISVPRIKHADDIDPDVFDNGISDDTFAKICALIRISVPYTGMIISTR
ESQTVREKVLPLGVSQISGASKTSVGGYADPEAEKNAEATSEQFDVSDQRTLDEVVNWLMKMDYIPSFCTACYREGRTGD
RFMALCKSMQILNCCHPNALMTLKEYLEDYASPQTRELGMKLIDREIEKITNPKVKQTCYEHIHDIADGKRDFRF

Sequences:

>Translated_475_residues
MYNVKSLKAEEFISDEEIKETLEYAEANKNNIGLIDAIIEKAKLKKGLTHREASVLLACEIPEKLDEVYKLAQQIKKDFY
GNRIVLFAPLYLSNYCVNGCVYCPYHMKNKHIARKKLTQEEIVKEVTALQDMGHKRLAIEAGEDPVNNPIEYILECINTI
YSIKHKNGAIRRVNVNIAATTVENYRKLKEAGIGTYILFQETYHKESYEQLHPTGPKHDYAYHTEAMDRAMEGGIDDVGL
GVLFGLDKYKYEFAGLLMHAEHLEAVHGVGPHTISVPRIKHADDIDPDVFDNGISDDTFAKICALIRISVPYTGMIISTR
ESQTVREKVLPLGVSQISGASKTSVGGYADPEAEKNAEATSEQFDVSDQRTLDEVVNWLMKMDYIPSFCTACYREGRTGD
RFMALCKSMQILNCCHPNALMTLKEYLEDYASPQTRELGMKLIDREIEKITNPKVKQTCYEHIHDIADGKRDFRF
>Mature_475_residues
MYNVKSLKAEEFISDEEIKETLEYAEANKNNIGLIDAIIEKAKLKKGLTHREASVLLACEIPEKLDEVYKLAQQIKKDFY
GNRIVLFAPLYLSNYCVNGCVYCPYHMKNKHIARKKLTQEEIVKEVTALQDMGHKRLAIEAGEDPVNNPIEYILECINTI
YSIKHKNGAIRRVNVNIAATTVENYRKLKEAGIGTYILFQETYHKESYEQLHPTGPKHDYAYHTEAMDRAMEGGIDDVGL
GVLFGLDKYKYEFAGLLMHAEHLEAVHGVGPHTISVPRIKHADDIDPDVFDNGISDDTFAKICALIRISVPYTGMIISTR
ESQTVREKVLPLGVSQISGASKTSVGGYADPEAEKNAEATSEQFDVSDQRTLDEVVNWLMKMDYIPSFCTACYREGRTGD
RFMALCKSMQILNCCHPNALMTLKEYLEDYASPQTRELGMKLIDREIEKITNPKVKQTCYEHIHDIADGKRDFRF

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=374, Percent_Identity=29.4117647058824, Blast_Score=164, Evalue=9e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 53899; Mature: 53899

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNVKSLKAEEFISDEEIKETLEYAEANKNNIGLIDAIIEKAKLKKGLTHREASVLLACE
CCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEC
IPEKLDEVYKLAQQIKKDFYGNRIVLFAPLYLSNYCVNGCVYCPYHMKNKHIARKKLTQE
CHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHCCEEECCCCCCCCHHHHHHCCHH
EIVKEVTALQDMGHKRLAIEAGEDPVNNPIEYILECINTIYSIKHKNGAIRRVNVNIAAT
HHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEH
TVENYRKLKEAGIGTYILFQETYHKESYEQLHPTGPKHDYAYHTEAMDRAMEGGIDDVGL
HHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHH
GVLFGLDKYKYEFAGLLMHAEHLEAVHGVGPHTISVPRIKHADDIDPDVFDNGISDDTFA
HHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHH
KICALIRISVPYTGMIISTRESQTVREKVLPLGVSQISGASKTSVGGYADPEAEKNAEAT
HHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCHHH
SEQFDVSDQRTLDEVVNWLMKMDYIPSFCTACYREGRTGDRFMALCKSMQILNCCHPNAL
HHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHH
MTLKEYLEDYASPQTRELGMKLIDREIEKITNPKVKQTCYEHIHDIADGKRDFRF
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MYNVKSLKAEEFISDEEIKETLEYAEANKNNIGLIDAIIEKAKLKKGLTHREASVLLACE
CCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEC
IPEKLDEVYKLAQQIKKDFYGNRIVLFAPLYLSNYCVNGCVYCPYHMKNKHIARKKLTQE
CHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHCCEEECCCCCCCCHHHHHHCCHH
EIVKEVTALQDMGHKRLAIEAGEDPVNNPIEYILECINTIYSIKHKNGAIRRVNVNIAAT
HHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEH
TVENYRKLKEAGIGTYILFQETYHKESYEQLHPTGPKHDYAYHTEAMDRAMEGGIDDVGL
HHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHH
GVLFGLDKYKYEFAGLLMHAEHLEAVHGVGPHTISVPRIKHADDIDPDVFDNGISDDTFA
HHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHH
KICALIRISVPYTGMIISTRESQTVREKVLPLGVSQISGASKTSVGGYADPEAEKNAEAT
HHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCHHH
SEQFDVSDQRTLDEVVNWLMKMDYIPSFCTACYREGRTGDRFMALCKSMQILNCCHPNAL
HHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHH
MTLKEYLEDYASPQTRELGMKLIDREIEKITNPKVKQTCYEHIHDIADGKRDFRF
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]