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The map label for this gene is bioB [H]

Identifier: 238922659

GI number: 238922659

Start: 215505

End: 216563

Strand: Direct

Name: bioB [H]

Synonym: EUBREC_0234

Alternate gene names: 238922659

Gene position: 215505-216563 (Clockwise)

Preceding gene: 238922658

Following gene: 238922660

Centisome position: 6.25

GC content: 49.01

Gene sequence:

>1059_bases
ATGACTGACAATACAGCAATAATAAAAATTATAGATGAAATTACGGCCTGCCGGAATATCACCCTCGAGCAGCTGCATAT
GCTGCTTGAAACGGATGATATGCAGGCAGTTGATTACCTTAGAAAGAGGGCATCTGAGAAAGCACACGAGACCTATGGCA
ATCAGGTTTTTATACGAGGGCTGATAGAGTTTACCAACTACTGCAAAAACGACTGTCTCTACTGCGGCATAAGGCACAGC
AATACTCACGCGGACCGCTACCGCCTGAGTACGGAGCAGATTATGGCATGCTGCGAGAGCGGCTATGAGCTTGGTTTTCG
TACCTTTGTGCTGCAGGGAGGTGAAGATCCGTATTACACGGATGAGCGCATCTGTGAGATAGTGTCCGGGATTAAGGCAA
AGTATCCGGACTGCGCAGTTACACTCTCTATCGGAGAAAAGTCAAAGGAGAGCTATCAGAGCTATTTTGATGCAGGGGCA
GACAGATATCTGCTGCGCCATGAGACTGCGGATGAAAAGCATTACAGTAGGCTTCATCCGGCAGAGATGTCACTTGAAAA
CAGAAAGCAGTGCCTTTGGGATTTGAAAGAGATAGGATATCAGGTGGGATGCGGCTTCATGGTCGGATCGCCGGGACAGA
CAGTGGAGACCTTGTACGAGGATTTGCAGTTTATAAAGGAGCTTGAGCCTCACATGGTTGGAATCGGTCCGTTCATATCG
CAGAAGGATACACCTTTTGCAAACGAGAAGTCAGGTACGATGGATGAGACGCTTCGCCTGCTTTCAATCATCAGGCTCAT
ACATCCAAAAGTGCTGCTGCCTGCCACAACAGCGCTTGGCACAATCCATCCGCTCGGCAGGGAAAAGGGTATACAGTCGG
GTGCCAATGTTGTGATGCCAAACCTCTCACCGGTCAATGTTCGTGACAAATACAAGCTCTACGACAATAAAATCTGCACC
GGAGATGAGGCGGCAGAGTGCCGCTACTGTATGGAAAACCGGATGAAGAGCATCGGCTATCAGGTGGTGGTGTCGCGTGG
GGACTACGCAAATTTTTGA

Upstream 100 bases:

>100_bases
ATTATCAGTGCTCTGTCCGGCAGGATTGGCAGGCTGGATGGCATAGGAGTGAAGACAGTTTACTCTAATAAATAAAGCAG
ACCAGACTACAGGAGAAAGC

Downstream 100 bases:

>100_bases
TTAATAGTTACGATTAATAAATATAAAACAACACCAGGATATAGAAGGAGATAAAAATGTATAACGTAAAATCATTAAAG
GCTGAAGAATTTATTTCAGA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MTDNTAIIKIIDEITACRNITLEQLHMLLETDDMQAVDYLRKRASEKAHETYGNQVFIRGLIEFTNYCKNDCLYCGIRHS
NTHADRYRLSTEQIMACCESGYELGFRTFVLQGGEDPYYTDERICEIVSGIKAKYPDCAVTLSIGEKSKESYQSYFDAGA
DRYLLRHETADEKHYSRLHPAEMSLENRKQCLWDLKEIGYQVGCGFMVGSPGQTVETLYEDLQFIKELEPHMVGIGPFIS
QKDTPFANEKSGTMDETLRLLSIIRLIHPKVLLPATTALGTIHPLGREKGIQSGANVVMPNLSPVNVRDKYKLYDNKICT
GDEAAECRYCMENRMKSIGYQVVVSRGDYANF

Sequences:

>Translated_352_residues
MTDNTAIIKIIDEITACRNITLEQLHMLLETDDMQAVDYLRKRASEKAHETYGNQVFIRGLIEFTNYCKNDCLYCGIRHS
NTHADRYRLSTEQIMACCESGYELGFRTFVLQGGEDPYYTDERICEIVSGIKAKYPDCAVTLSIGEKSKESYQSYFDAGA
DRYLLRHETADEKHYSRLHPAEMSLENRKQCLWDLKEIGYQVGCGFMVGSPGQTVETLYEDLQFIKELEPHMVGIGPFIS
QKDTPFANEKSGTMDETLRLLSIIRLIHPKVLLPATTALGTIHPLGREKGIQSGANVVMPNLSPVNVRDKYKLYDNKICT
GDEAAECRYCMENRMKSIGYQVVVSRGDYANF
>Mature_351_residues
TDNTAIIKIIDEITACRNITLEQLHMLLETDDMQAVDYLRKRASEKAHETYGNQVFIRGLIEFTNYCKNDCLYCGIRHSN
THADRYRLSTEQIMACCESGYELGFRTFVLQGGEDPYYTDERICEIVSGIKAKYPDCAVTLSIGEKSKESYQSYFDAGAD
RYLLRHETADEKHYSRLHPAEMSLENRKQCLWDLKEIGYQVGCGFMVGSPGQTVETLYEDLQFIKELEPHMVGIGPFISQ
KDTPFANEKSGTMDETLRLLSIIRLIHPKVLLPATTALGTIHPLGREKGIQSGANVVMPNLSPVNVRDKYKLYDNKICTG
DEAAECRYCMENRMKSIGYQVVVSRGDYANF

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 40008; Mature: 39877

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDNTAIIKIIDEITACRNITLEQLHMLLETDDMQAVDYLRKRASEKAHETYGNQVFIRG
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
LIEFTNYCKNDCLYCGIRHSNTHADRYRLSTEQIMACCESGYELGFRTFVLQGGEDPYYT
HHHHHHHHCCCCEEEEECCCCCCHHHHHCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCC
DERICEIVSGIKAKYPDCAVTLSIGEKSKESYQSYFDAGADRYLLRHETADEKHYSRLHP
HHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEHHCCCCCHHHHHHCCC
AEMSLENRKQCLWDLKEIGYQVGCGFMVGSPGQTVETLYEDLQFIKELEPHMVGIGPFIS
HHHCHHHHHHHHHHHHHCCHHHCCCEEECCCCHHHHHHHHHHHHHHHHCCCEEECCCCCC
QKDTPFANEKSGTMDETLRLLSIIRLIHPKVLLPATTALGTIHPLGREKGIQSGANVVMP
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHCCCCCCHHHCCCCCCCEECC
NLSPVNVRDKYKLYDNKICTGDEAAECRYCMENRMKSIGYQVVVSRGDYANF
CCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCC
>Mature Secondary Structure 
TDNTAIIKIIDEITACRNITLEQLHMLLETDDMQAVDYLRKRASEKAHETYGNQVFIRG
CCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
LIEFTNYCKNDCLYCGIRHSNTHADRYRLSTEQIMACCESGYELGFRTFVLQGGEDPYYT
HHHHHHHHCCCCEEEEECCCCCCHHHHHCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCC
DERICEIVSGIKAKYPDCAVTLSIGEKSKESYQSYFDAGADRYLLRHETADEKHYSRLHP
HHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEHHCCCCCHHHHHHCCC
AEMSLENRKQCLWDLKEIGYQVGCGFMVGSPGQTVETLYEDLQFIKELEPHMVGIGPFIS
HHHCHHHHHHHHHHHHHCCHHHCCCEEECCCCHHHHHHHHHHHHHHHHCCCEEECCCCCC
QKDTPFANEKSGTMDETLRLLSIIRLIHPKVLLPATTALGTIHPLGREKGIQSGANVVMP
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHCCCCCCHHHCCCCCCCEECC
NLSPVNVRDKYKLYDNKICTGDEAAECRYCMENRMKSIGYQVVVSRGDYANF
CCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA