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The map label for this gene is mnmE [H]

Identifier: 238922661

GI number: 238922661

Start: 218010

End: 219374

Strand: Direct

Name: mnmE [H]

Synonym: EUBREC_0236

Alternate gene names: 238922661

Gene position: 218010-219374 (Clockwise)

Preceding gene: 238922660

Following gene: 238922662

Centisome position: 6.32

GC content: 47.4

Gene sequence:

>1365_bases
ATGATATTGCAGACGGAAAGCGTGATTTCCGTTTCTAAAGGAGGAAATATGGGAAATTTGAATGAGACGCCTTCTGCCAA
CAGACTTCATATAGGTATATACGGAAAGACAAACAGTGGTAAGTCATCTCTGATAAATGCGGTTACAAAGCAGGAGGTTT
CCATTGTAGCTGACGTGGCAGGTACGACAACCGACCCTGTGTACAAGCCGATGGAGATACATCCGCTCGGACCGTGCGTG
ATTATTGATACGGCGGGCTTTGATGATGACAGTGAGCTTGGTGAAAGACGAGTGGAAAAGACCCATCTGGCAGCAGAGAA
GACAGACATAGCCGTTGTAGTGCTTGATATTGCAGAGGTCATTGCTGCAAAGAAGGCAGGGGTACCATTTAAAAAGGCTT
TTAAGGATGAGGCAGAGTGGAGCCTGCTATTTGCAAAAAAACATACTCCGGTTGTATTTGCCTTAAACAAGATAGACGAG
ATTTTGCTTTCCGCAGAGGCTCAGGAAAAGAGCAGGGGAAAGCTTGACAATGCTGCAATAGAGGCCGCGAAGTGCTGGGT
TTATGAGGAAAACAGTGCTGTGATGGGCAAAAATGCAGACAATAGCTTTGAGGTTGTAGCAGTCAGCGCATTAAAGGGCA
AGGGAATGGATGCCGTTATCTCTGCACTCACACGCCTTTTGCCTGAGGATTTCGGACAGGAATTTATCCTGGGAGACCTT
GTTTCTCAGACAGACCTGGTGCTTCTTGTTATGCCGCAGGATATCCAGGCGCCAAAGGGCAGACTCATATTGCCACAGGT
GCAGACACTCAGGGAATTGCTCGATAGAAAATGTCTTGTGATGAGCACGACGACTGACAAAATGACAGATGCGCTTGCAG
CATTATCTCACGCGCCAAAGCTCATCGTGACAGATTCACAGGTGTTTGGCTATGTGTACGAGCACAAACCGGCGGAGAGT
ATGCTCACATCATTTTCAGTGCTTTTTGCAGCATACAAGGGTGATCTGCCATATTATGTGGAGAGCGCAAGGGCAATTGA
TGCCTTAAAGCCATCCTCAAGAGTGCTTATCGCAGAGTGCTGTACACATGCGCCTCTTGCAGAGGATATAGGCAGGGTGA
AGCTTCCGAACCTGCTTCGAAAGCGTGTCGGTGAGGAGCTTACCGTGGATATTACAAGCGGTACAGACTTCCCTGATAAT
CTTTCTCAGTACGATCTCGTTATTCAGTGCGGTGCTTGCATGTTTAACAGAAAATATGTATTATCAAGAATAGACAGGGC
ACGCACTCAGCATGTGCCGATGACAAACTATGGCGTGGCTATAGCACACCTGACAGGAATACTCGATAAGGTGAGCATGC
CCTAG

Upstream 100 bases:

>100_bases
GCAAGCCCGCAGACAAGAGAGCTTGGAATGAAGCTCATCGATAGGGAAATAGAAAAGATTACCAATCCAAAGGTTAAGCA
GACCTGCTATGAGCATATAC

Downstream 100 bases:

>100_bases
AAAAACCTTAAAAGCCGGGAGGAAGAGAAGCTATCGGTATGACGAAAACAAGCGATTTTGACGACAAAGTGAATTATAGT
GGCGATTATAAAGGCAATTA

Product: GTP-binding protein EngA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MILQTESVISVSKGGNMGNLNETPSANRLHIGIYGKTNSGKSSLINAVTKQEVSIVADVAGTTTDPVYKPMEIHPLGPCV
IIDTAGFDDDSELGERRVEKTHLAAEKTDIAVVVLDIAEVIAAKKAGVPFKKAFKDEAEWSLLFAKKHTPVVFALNKIDE
ILLSAEAQEKSRGKLDNAAIEAAKCWVYEENSAVMGKNADNSFEVVAVSALKGKGMDAVISALTRLLPEDFGQEFILGDL
VSQTDLVLLVMPQDIQAPKGRLILPQVQTLRELLDRKCLVMSTTTDKMTDALAALSHAPKLIVTDSQVFGYVYEHKPAES
MLTSFSVLFAAYKGDLPYYVESARAIDALKPSSRVLIAECCTHAPLAEDIGRVKLPNLLRKRVGEELTVDITSGTDFPDN
LSQYDLVIQCGACMFNRKYVLSRIDRARTQHVPMTNYGVAIAHLTGILDKVSMP

Sequences:

>Translated_454_residues
MILQTESVISVSKGGNMGNLNETPSANRLHIGIYGKTNSGKSSLINAVTKQEVSIVADVAGTTTDPVYKPMEIHPLGPCV
IIDTAGFDDDSELGERRVEKTHLAAEKTDIAVVVLDIAEVIAAKKAGVPFKKAFKDEAEWSLLFAKKHTPVVFALNKIDE
ILLSAEAQEKSRGKLDNAAIEAAKCWVYEENSAVMGKNADNSFEVVAVSALKGKGMDAVISALTRLLPEDFGQEFILGDL
VSQTDLVLLVMPQDIQAPKGRLILPQVQTLRELLDRKCLVMSTTTDKMTDALAALSHAPKLIVTDSQVFGYVYEHKPAES
MLTSFSVLFAAYKGDLPYYVESARAIDALKPSSRVLIAECCTHAPLAEDIGRVKLPNLLRKRVGEELTVDITSGTDFPDN
LSQYDLVIQCGACMFNRKYVLSRIDRARTQHVPMTNYGVAIAHLTGILDKVSMP
>Mature_454_residues
MILQTESVISVSKGGNMGNLNETPSANRLHIGIYGKTNSGKSSLINAVTKQEVSIVADVAGTTTDPVYKPMEIHPLGPCV
IIDTAGFDDDSELGERRVEKTHLAAEKTDIAVVVLDIAEVIAAKKAGVPFKKAFKDEAEWSLLFAKKHTPVVFALNKIDE
ILLSAEAQEKSRGKLDNAAIEAAKCWVYEENSAVMGKNADNSFEVVAVSALKGKGMDAVISALTRLLPEDFGQEFILGDL
VSQTDLVLLVMPQDIQAPKGRLILPQVQTLRELLDRKCLVMSTTTDKMTDALAALSHAPKLIVTDSQVFGYVYEHKPAES
MLTSFSVLFAAYKGDLPYYVESARAIDALKPSSRVLIAECCTHAPLAEDIGRVKLPNLLRKRVGEELTVDITSGTDFPDN
LSQYDLVIQCGACMFNRKYVLSRIDRARTQHVPMTNYGVAIAHLTGILDKVSMP

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG1160

COG function: function code R; Predicted GTPases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49421; Mature: 49421

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILQTESVISVSKGGNMGNLNETPSANRLHIGIYGKTNSGKSSLINAVTKQEVSIVADVA
CEECCCCEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHEEEECC
GTTTDPVYKPMEIHPLGPCVIIDTAGFDDDSELGERRVEKTHLAAEKTDIAVVVLDIAEV
CCCCCCCCCCEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHH
IAAKKAGVPFKKAFKDEAEWSLLFAKKHTPVVFALNKIDEILLSAEAQEKSRGKLDNAAI
HHHHHCCCCHHHHHCCCCCEEEEEEECCCCEEEEHHHHHHHHHHCCHHHHHCCCCHHHHH
EAAKCWVYEENSAVMGKNADNSFEVVAVSALKGKGMDAVISALTRLLPEDFGQEFILGDL
HHEEEEEEECCCEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
VSQTDLVLLVMPQDIQAPKGRLILPQVQTLRELLDRKCLVMSTTTDKMTDALAALSHAPK
CCCCCEEEEEECCCCCCCCCCEECHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCE
LIVTDSQVFGYVYEHKPAESMLTSFSVLFAAYKGDLPYYVESARAIDALKPSSRVLIAEC
EEEECHHHEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEHHHH
CTHAPLAEDIGRVKLPNLLRKRVGEELTVDITSGTDFPDNLSQYDLVIQCGACMFNRKYV
HCCCCCHHHHCCCCCHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEEEEECHHHHHHHHH
LSRIDRARTQHVPMTNYGVAIAHLTGILDKVSMP
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MILQTESVISVSKGGNMGNLNETPSANRLHIGIYGKTNSGKSSLINAVTKQEVSIVADVA
CEECCCCEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHEEEECC
GTTTDPVYKPMEIHPLGPCVIIDTAGFDDDSELGERRVEKTHLAAEKTDIAVVVLDIAEV
CCCCCCCCCCEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHH
IAAKKAGVPFKKAFKDEAEWSLLFAKKHTPVVFALNKIDEILLSAEAQEKSRGKLDNAAI
HHHHHCCCCHHHHHCCCCCEEEEEEECCCCEEEEHHHHHHHHHHCCHHHHHCCCCHHHHH
EAAKCWVYEENSAVMGKNADNSFEVVAVSALKGKGMDAVISALTRLLPEDFGQEFILGDL
HHEEEEEEECCCEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
VSQTDLVLLVMPQDIQAPKGRLILPQVQTLRELLDRKCLVMSTTTDKMTDALAALSHAPK
CCCCCEEEEEECCCCCCCCCCEECHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCE
LIVTDSQVFGYVYEHKPAESMLTSFSVLFAAYKGDLPYYVESARAIDALKPSSRVLIAEC
EEEECHHHEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEHHHH
CTHAPLAEDIGRVKLPNLLRKRVGEELTVDITSGTDFPDNLSQYDLVIQCGACMFNRKYV
HCCCCCHHHHCCCCCHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEEEEECHHHHHHHHH
LSRIDRARTQHVPMTNYGVAIAHLTGILDKVSMP
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA