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The map label for this gene is gyrB [H]

Identifier: 238922437

GI number: 238922437

Start: 4065

End: 6002

Strand: Direct

Name: gyrB [H]

Synonym: EUBREC_0005

Alternate gene names: 238922437

Gene position: 4065-6002 (Clockwise)

Preceding gene: 238922436

Following gene: 238922438

Centisome position: 0.12

GC content: 44.69

Gene sequence:

>1938_bases
ATGAGTAAAGAAATACAGCAGAACAATCAGGAATACGGAGCTGACCAGATTCAGATACTTGAGGGTCTTGAGGCGGTCAG
AAAAAGACCGGGTATGTATATCGGCAGTACGTCTTCGAGAGGACTTCATCATCTGGTTTACGAGATAGTGGACAATGCAG
TTGATGAGGCCTTAGCCGGTTATTGCAAAAATATCGAGGTCACAATCGAGGAAGACAATTCCATAACAGTACAGGATGAT
GGACGAGGAATCCCGGTCGGAACCAATCATAAAGCAAAAAAATCAGCACTTGAGGTCGTTTTTACAGTGCTTCACGCAGG
TGGTAAATTCGGCGGTGGAGGCTATAAGGTATCAGGCGGTCTGCACGGAGTTGGTGCATCTGTAGTAAATGCTCTTTCCA
CATGGCTGACAGTTGAGGTCTACAAGGACGGAAATATTTACAGACAAAGCTACAAGCGTGGCAAAACAGACGATACAGTA
AAGATTGTTGGACAGTGTGACGAGTCGCTTCATGGTACAAAGGTGCATTTTCTGCCGGATCCGGAGATTTTTGAGGAGAC
AGTGTATGACTACGATACCTTAAAGCAGCGTCTCAGAGAGACAGCCTTCCTCACAAAAGGACTTAAGATTACTTTAAAGG
ATGTGCGTGAGAATAGCCATCACAATCATGTATTCCACTATGAGGGCGGAATCAAGGAATTTGTAGAGTATCTTAACCGT
GGCAAGGAGGCTCTCTATGATGAGGTCATCTACTGCGAGGGAACACAAAATGGTGTTTATGTTGAGGTTGCATTGCAGCA
CAATGATTCGTTTAATGATTCAACCTTCAGCTTTGTAAACAATGTAATCACACCTGAGGGAGGTACTCATCTCGCAGGCT
TTAGAAATGCCTTGACAAAGACCTTCAATGCCTATGCGCGTGAGAAAAAGATACTTAAGGATTCAGATCCGGCCCTGACA
GGTGATGATATCCGTGAGGGACTTACTGCAATCATCAGTATCAAGATTGAGGAGCCTCAGTTCGAGGGACAGACAAAGCA
GAAGCTTGGAAACAGTGAGGCCAGAGGAGCCGTAGATGCTGTTGTATCGGAAAAACTCACATATTTCCTCGAGCAGAATC
CTGATGTAGCCAAAAATATCTGTGAGAAATCACTTTTAGCACAAAGAGCCAGAGAGGCCGCAAGAAAAGCAAGAGACCTG
ACCAGAAGAAAGACCTCTCTCGATGGAGGTACTCTGCCGGGCAAGCTGGCTGACTGCTCAGACAAGGATCCTAAAAACTG
TGAAATTTTCATAGTCGAGGGAGATTCAGCCGGCGGTTCTGCAAAGACAGCAAGGAGCCGTGCTACACAGGCAATTTTAC
CTCTGCGAGGCAAGATACTGAATGTTGAAAAGGCAAGACTTGACCGTATATATGACAATGCAGAAATCAGAGCCATGATT
ACAGCATTCGGAACAGGAATCCATGAGGATTTTGATATAACAAAGCTGCGTTATGACAAGATAATCATCATGACAGATGC
CGATGTAGATGGTGCTCACATCGCTACTCTCATGCTCACCTTCCTCTACAGATTCATGCCTGATCTGATCAAGGAGGGAC
ATGTATACCTGGCTACACCGCCACTTTACAAGGTGGAGAAAAACAAGGGTGTATGGTATGCCTACGATGATGACGAGCTC
AAGCAGATTTTAAATGACATAGGCCGTGACAATAATAACAAGATCCAGCGATACAAGGGACTTGGCGAGATGGATGCTGA
TCAGCTCTGGGAGACAACAATGGATCCTGACCACAGAATTTTGAAACAGGTCATGATAGACGGAGAAAATTCATCTGAGC
TTGATGTGACATTTACCACACTGATGGGTGATAAGGTAGAGCCAAGAAAAGAATTTATTGAAGCTAATGCCAAGTATGTA
ACAAATCTTGACATCTAG

Upstream 100 bases:

>100_bases
CAGACCATTATCACTTGTACGGGACTTGATGAATTTGTAAAAAATAGATTTCAGGTGGACAAGGTCTTTCATGTCGTGAA
GGGCCAGGTGGAGGTTATTG

Downstream 100 bases:

>100_bases
CAGGGAGGAGTAATTTAAGTGGACGACAAAATTTTTGACCAGATACAGCAGGTCGATTTGAAAAAAACAATGGAGAGCTC
CTACATAGATTACGCGATGA

Product: DNA gyrase, B subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 645; Mature: 644

Protein sequence:

>645_residues
MSKEIQQNNQEYGADQIQILEGLEAVRKRPGMYIGSTSSRGLHHLVYEIVDNAVDEALAGYCKNIEVTIEEDNSITVQDD
GRGIPVGTNHKAKKSALEVVFTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSTWLTVEVYKDGNIYRQSYKRGKTDDTV
KIVGQCDESLHGTKVHFLPDPEIFEETVYDYDTLKQRLRETAFLTKGLKITLKDVRENSHHNHVFHYEGGIKEFVEYLNR
GKEALYDEVIYCEGTQNGVYVEVALQHNDSFNDSTFSFVNNVITPEGGTHLAGFRNALTKTFNAYAREKKILKDSDPALT
GDDIREGLTAIISIKIEEPQFEGQTKQKLGNSEARGAVDAVVSEKLTYFLEQNPDVAKNICEKSLLAQRAREAARKARDL
TRRKTSLDGGTLPGKLADCSDKDPKNCEIFIVEGDSAGGSAKTARSRATQAILPLRGKILNVEKARLDRIYDNAEIRAMI
TAFGTGIHEDFDITKLRYDKIIIMTDADVDGAHIATLMLTFLYRFMPDLIKEGHVYLATPPLYKVEKNKGVWYAYDDDEL
KQILNDIGRDNNNKIQRYKGLGEMDADQLWETTMDPDHRILKQVMIDGENSSELDVTFTTLMGDKVEPRKEFIEANAKYV
TNLDI

Sequences:

>Translated_645_residues
MSKEIQQNNQEYGADQIQILEGLEAVRKRPGMYIGSTSSRGLHHLVYEIVDNAVDEALAGYCKNIEVTIEEDNSITVQDD
GRGIPVGTNHKAKKSALEVVFTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSTWLTVEVYKDGNIYRQSYKRGKTDDTV
KIVGQCDESLHGTKVHFLPDPEIFEETVYDYDTLKQRLRETAFLTKGLKITLKDVRENSHHNHVFHYEGGIKEFVEYLNR
GKEALYDEVIYCEGTQNGVYVEVALQHNDSFNDSTFSFVNNVITPEGGTHLAGFRNALTKTFNAYAREKKILKDSDPALT
GDDIREGLTAIISIKIEEPQFEGQTKQKLGNSEARGAVDAVVSEKLTYFLEQNPDVAKNICEKSLLAQRAREAARKARDL
TRRKTSLDGGTLPGKLADCSDKDPKNCEIFIVEGDSAGGSAKTARSRATQAILPLRGKILNVEKARLDRIYDNAEIRAMI
TAFGTGIHEDFDITKLRYDKIIIMTDADVDGAHIATLMLTFLYRFMPDLIKEGHVYLATPPLYKVEKNKGVWYAYDDDEL
KQILNDIGRDNNNKIQRYKGLGEMDADQLWETTMDPDHRILKQVMIDGENSSELDVTFTTLMGDKVEPRKEFIEANAKYV
TNLDI
>Mature_644_residues
SKEIQQNNQEYGADQIQILEGLEAVRKRPGMYIGSTSSRGLHHLVYEIVDNAVDEALAGYCKNIEVTIEEDNSITVQDDG
RGIPVGTNHKAKKSALEVVFTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSTWLTVEVYKDGNIYRQSYKRGKTDDTVK
IVGQCDESLHGTKVHFLPDPEIFEETVYDYDTLKQRLRETAFLTKGLKITLKDVRENSHHNHVFHYEGGIKEFVEYLNRG
KEALYDEVIYCEGTQNGVYVEVALQHNDSFNDSTFSFVNNVITPEGGTHLAGFRNALTKTFNAYAREKKILKDSDPALTG
DDIREGLTAIISIKIEEPQFEGQTKQKLGNSEARGAVDAVVSEKLTYFLEQNPDVAKNICEKSLLAQRAREAARKARDLT
RRKTSLDGGTLPGKLADCSDKDPKNCEIFIVEGDSAGGSAKTARSRATQAILPLRGKILNVEKARLDRIYDNAEIRAMIT
AFGTGIHEDFDITKLRYDKIIIMTDADVDGAHIATLMLTFLYRFMPDLIKEGHVYLATPPLYKVEKNKGVWYAYDDDELK
QILNDIGRDNNNKIQRYKGLGEMDADQLWETTMDPDHRILKQVMIDGENSSELDVTFTTLMGDKVEPRKEFIEANAKYVT
NLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=663, Percent_Identity=27.1493212669683, Blast_Score=153, Evalue=5e-37,
Organism=Homo sapiens, GI19913406, Length=657, Percent_Identity=26.4840182648402, Blast_Score=146, Evalue=5e-35,
Organism=Escherichia coli, GI48994957, Length=562, Percent_Identity=52.846975088968, Blast_Score=564, Evalue=1e-162,
Organism=Escherichia coli, GI1789408, Length=629, Percent_Identity=38.9507154213037, Blast_Score=430, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17535065, Length=663, Percent_Identity=28.5067873303167, Blast_Score=182, Evalue=7e-46,
Organism=Caenorhabditis elegans, GI212645845, Length=601, Percent_Identity=27.1214642262895, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI212645657, Length=197, Percent_Identity=31.9796954314721, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324241, Length=670, Percent_Identity=27.3134328358209, Blast_Score=140, Evalue=7e-34,
Organism=Drosophila melanogaster, GI17136538, Length=627, Percent_Identity=26.9537480063796, Blast_Score=156, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72089; Mature: 71958

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEIQQNNQEYGADQIQILEGLEAVRKRPGMYIGSTSSRGLHHLVYEIVDNAVDEALAG
CCCHHHHCCHHHCCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
YCKNIEVTIEEDNSITVQDDGRGIPVGTNHKAKKSALEVVFTVLHAGGKFGGGGYKVSGG
HHCCCEEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCC
LHGVGASVVNALSTWLTVEVYKDGNIYRQSYKRGKTDDTVKIVGQCDESLHGTKVHFLPD
CCCHHHHHHHHHHHHEEEEEEECCCHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEECCC
PEIFEETVYDYDTLKQRLRETAFLTKGLKITLKDVRENSHHNHVFHYEGGIKEFVEYLNR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCCEEEEECCCHHHHHHHHHC
GKEALYDEVIYCEGTQNGVYVEVALQHNDSFNDSTFSFVNNVITPEGGTHLAGFRNALTK
CHHHHHHCEEEEECCCCCEEEEEEEECCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHH
TFNAYAREKKILKDSDPALTGDDIREGLTAIISIKIEEPQFEGQTKQKLGNSEARGAVDA
HHHHHHHHHHHHCCCCCCCCHHHHHHCCEEEEEEEEECCCCCCCHHHHCCCCHHHHHHHH
VVSEKLTYFLEQNPDVAKNICEKSLLAQRAREAARKARDLTRRKTSLDGGTLPGKLADCS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
DKDPKNCEIFIVEGDSAGGSAKTARSRATQAILPLRGKILNVEKARLDRIYDNAEIRAMI
CCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEECHHHHHHHHHHCCCHHEEHH
TAFGTGIHEDFDITKLRYDKIIIMTDADVDGAHIATLMLTFLYRFMPDLIKEGHVYLATP
HHHCCCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
PLYKVEKNKGVWYAYDDDELKQILNDIGRDNNNKIQRYKGLGEMDADQLWETTMDPDHRI
CCEEEECCCCEEEEECHHHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHCCCCHHHHH
LKQVMIDGENSSELDVTFTTLMGDKVEPRKEFIEANAKYVTNLDI
HHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHCCCCEEEECCC
>Mature Secondary Structure 
SKEIQQNNQEYGADQIQILEGLEAVRKRPGMYIGSTSSRGLHHLVYEIVDNAVDEALAG
CCHHHHCCHHHCCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
YCKNIEVTIEEDNSITVQDDGRGIPVGTNHKAKKSALEVVFTVLHAGGKFGGGGYKVSGG
HHCCCEEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCC
LHGVGASVVNALSTWLTVEVYKDGNIYRQSYKRGKTDDTVKIVGQCDESLHGTKVHFLPD
CCCHHHHHHHHHHHHEEEEEEECCCHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEECCC
PEIFEETVYDYDTLKQRLRETAFLTKGLKITLKDVRENSHHNHVFHYEGGIKEFVEYLNR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCCEEEEECCCHHHHHHHHHC
GKEALYDEVIYCEGTQNGVYVEVALQHNDSFNDSTFSFVNNVITPEGGTHLAGFRNALTK
CHHHHHHCEEEEECCCCCEEEEEEEECCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHH
TFNAYAREKKILKDSDPALTGDDIREGLTAIISIKIEEPQFEGQTKQKLGNSEARGAVDA
HHHHHHHHHHHHCCCCCCCCHHHHHHCCEEEEEEEEECCCCCCCHHHHCCCCHHHHHHHH
VVSEKLTYFLEQNPDVAKNICEKSLLAQRAREAARKARDLTRRKTSLDGGTLPGKLADCS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
DKDPKNCEIFIVEGDSAGGSAKTARSRATQAILPLRGKILNVEKARLDRIYDNAEIRAMI
CCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEECHHHHHHHHHHCCCHHEEHH
TAFGTGIHEDFDITKLRYDKIIIMTDADVDGAHIATLMLTFLYRFMPDLIKEGHVYLATP
HHHCCCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
PLYKVEKNKGVWYAYDDDELKQILNDIGRDNNNKIQRYKGLGEMDADQLWETTMDPDHRI
CCEEEECCCCEEEEECHHHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHCCCCHHHHH
LKQVMIDGENSSELDVTFTTLMGDKVEPRKEFIEANAKYVTNLDI
HHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]