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The map label for this gene is recF

Identifier: 238922436

GI number: 238922436

Start: 2984

End: 4072

Strand: Direct

Name: recF

Synonym: EUBREC_0004

Alternate gene names: 238922436

Gene position: 2984-4072 (Clockwise)

Preceding gene: 238922435

Following gene: 238922437

Centisome position: 0.09

GC content: 41.51

Gene sequence:

>1089_bases
ATGATTATTAAGTCAATCCAACTGAGTAATTTCAGAAATTACGAAAAACTGGATATCAGCTTTGACACTGAAACCAACAT
AATCTACGGCGATAATGCCCAGGGCAAGACCAATATACTTGAGGCTGCATATTTGAGCGGCACTACAAAGTCCCATAAGG
GCAGCAAGGACAAGGAAATGATCCGGTTTGGCGAGGATGAGGCACATATCAGGACAATTGTTGAAAAAAATGACAAGGAA
TACCGGATTGACATGCATCTGCGAAAAAACGGTGCAAAGGGTGTGGCCATCAATAAAATGCCGATCAAAAAAGCGAGTGA
GCTGTTTGGTATTTTGAATATTGTGTTTTTCTCTCCTGAGGATCTCAATATTATCAAAAACGGTCCTGCCGAGAGACGCA
GATTTATAGATTTGGAGCTTTGCCAGCTTGACAAAATATATCTGTCTAACCTGAGTAAGTATAATAAGACATTAGTTCAG
AGAAACAGACTGTTAAAGGATATAGCTTACAGACCTGATTTGATTGATACACTTCAGGTGTGGGATATGCAGCTTCTCGA
GTATGGCAGGCATGTTATAAAAAAGAGGCGCGAGTTTGTTAATGAGCTAAATGAGATAATACAGGACATTCACAGCAATA
TTTCAGGAGGCAGGGAGAAGCTTATACTCAAATATGAGCCAAGCATTGATGATATTTTTTTCGAGGACGAGCTGCTTAAG
GCAAGAAGCAGGGATTTGAAGCTGTGCCAGACAACCGTTGGACCGCACAGGGATGATATGCTTTTTTCAGTGGACGGTGT
GGATATCAGAAAATACGGCTCGCAGGGTCAGCAGAGGACATCTGCTCTCTCACTCAAGCTTTCTGAGATTAGTCTTGTAA
AGAAAAATATAAACAGCACTCCCGTGCTTTTACTGGATGATGTGCTTTCAGAGCTGGATGGCAACAGGCAGAACTATCTG
CTCAACAGCTTAAGTGATACACAGACCATTATCACTTGTACGGGACTTGATGAATTTGTAAAAAATAGATTTCAGGTGGA
CAAGGTCTTTCATGTCGTGAAGGGCCAGGTGGAGGTTATTGATGAGTAA

Upstream 100 bases:

>100_bases
AGGTGACTGTAAACGGTGAGACTGAGCTTCAGAGAGGAAAAAAGCTTCACGACGGAGATGTTTTCTCATATGACGGCGAA
ACAGTTAAGGTTGTTAAATA

Downstream 100 bases:

>100_bases
AGAAATACAGCAGAACAATCAGGAATACGGAGCTGACCAGATTCAGATACTTGAGGGTCTTGAGGCGGTCAGAAAAAGAC
CGGGTATGTATATCGGCAGT

Product: DNA replication and repair protein RecF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MIIKSIQLSNFRNYEKLDISFDTETNIIYGDNAQGKTNILEAAYLSGTTKSHKGSKDKEMIRFGEDEAHIRTIVEKNDKE
YRIDMHLRKNGAKGVAINKMPIKKASELFGILNIVFFSPEDLNIIKNGPAERRRFIDLELCQLDKIYLSNLSKYNKTLVQ
RNRLLKDIAYRPDLIDTLQVWDMQLLEYGRHVIKKRREFVNELNEIIQDIHSNISGGREKLILKYEPSIDDIFFEDELLK
ARSRDLKLCQTTVGPHRDDMLFSVDGVDIRKYGSQGQQRTSALSLKLSEISLVKKNINSTPVLLLDDVLSELDGNRQNYL
LNSLSDTQTIITCTGLDEFVKNRFQVDKVFHVVKGQVEVIDE

Sequences:

>Translated_362_residues
MIIKSIQLSNFRNYEKLDISFDTETNIIYGDNAQGKTNILEAAYLSGTTKSHKGSKDKEMIRFGEDEAHIRTIVEKNDKE
YRIDMHLRKNGAKGVAINKMPIKKASELFGILNIVFFSPEDLNIIKNGPAERRRFIDLELCQLDKIYLSNLSKYNKTLVQ
RNRLLKDIAYRPDLIDTLQVWDMQLLEYGRHVIKKRREFVNELNEIIQDIHSNISGGREKLILKYEPSIDDIFFEDELLK
ARSRDLKLCQTTVGPHRDDMLFSVDGVDIRKYGSQGQQRTSALSLKLSEISLVKKNINSTPVLLLDDVLSELDGNRQNYL
LNSLSDTQTIITCTGLDEFVKNRFQVDKVFHVVKGQVEVIDE
>Mature_362_residues
MIIKSIQLSNFRNYEKLDISFDTETNIIYGDNAQGKTNILEAAYLSGTTKSHKGSKDKEMIRFGEDEAHIRTIVEKNDKE
YRIDMHLRKNGAKGVAINKMPIKKASELFGILNIVFFSPEDLNIIKNGPAERRRFIDLELCQLDKIYLSNLSKYNKTLVQ
RNRLLKDIAYRPDLIDTLQVWDMQLLEYGRHVIKKRREFVNELNEIIQDIHSNISGGREKLILKYEPSIDDIFFEDELLK
ARSRDLKLCQTTVGPHRDDMLFSVDGVDIRKYGSQGQQRTSALSLKLSEISLVKKNINSTPVLLLDDVLSELDGNRQNYL
LNSLSDTQTIITCTGLDEFVKNRFQVDKVFHVVKGQVEVIDE

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=360, Percent_Identity=23.0555555555556, Blast_Score=115, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_EUBR3 (C4Z940)

Other databases:

- EMBL:   CP001107
- RefSeq:   YP_002935949.1
- GeneID:   7963230
- GenomeReviews:   CP001107_GR
- KEGG:   ere:EUBREC_0004
- ProtClustDB:   CLSK2505978
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 41717; Mature: 41717

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIKSIQLSNFRNYEKLDISFDTETNIIYGDNAQGKTNILEAAYLSGTTKSHKGSKDKEM
CEEECEECCCCCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCHHH
IRFGEDEAHIRTIVEKNDKEYRIDMHLRKNGAKGVAINKMPIKKASELFGILNIVFFSPE
HHCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHEEECCC
DLNIIKNGPAERRRFIDLELCQLDKIYLSNLSKYNKTLVQRNRLLKDIAYRPDLIDTLQV
CCCEECCCCHHHHHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
WDMQLLEYGRHVIKKRREFVNELNEIIQDIHSNISGGREKLILKYEPSIDDIFFEDELLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEECHHHHHH
ARSRDLKLCQTTVGPHRDDMLFSVDGVDIRKYGSQGQQRTSALSLKLSEISLVKKNINST
HHCCCHHHHHHCCCCCCCCEEEEECCCHHHHHCCCCHHHHHHHEEEHHHHHHHHHCCCCC
PVLLLDDVLSELDGNRQNYLLNSLSDTQTIITCTGLDEFVKNRFQVDKVFHVVKGQVEVI
CEEHHHHHHHHHCCCCHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEE
DE
CC
>Mature Secondary Structure
MIIKSIQLSNFRNYEKLDISFDTETNIIYGDNAQGKTNILEAAYLSGTTKSHKGSKDKEM
CEEECEECCCCCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCHHH
IRFGEDEAHIRTIVEKNDKEYRIDMHLRKNGAKGVAINKMPIKKASELFGILNIVFFSPE
HHCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHEEECCC
DLNIIKNGPAERRRFIDLELCQLDKIYLSNLSKYNKTLVQRNRLLKDIAYRPDLIDTLQV
CCCEECCCCHHHHHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
WDMQLLEYGRHVIKKRREFVNELNEIIQDIHSNISGGREKLILKYEPSIDDIFFEDELLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEECHHHHHH
ARSRDLKLCQTTVGPHRDDMLFSVDGVDIRKYGSQGQQRTSALSLKLSEISLVKKNINST
HHCCCHHHHHHCCCCCCCCEEEEECCCHHHHHCCCCHHHHHHHEEEHHHHHHHHHCCCCC
PVLLLDDVLSELDGNRQNYLLNSLSDTQTIITCTGLDEFVKNRFQVDKVFHVVKGQVEVI
CEEHHHHHHHHHCCCCHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEE
DE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA