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The map label for this gene is gdhA [H]

Identifier: 238921579

GI number: 238921579

Start: 3617978

End: 3619324

Strand: Direct

Name: gdhA [H]

Synonym: NT01EI_3732

Alternate gene names: 238921579

Gene position: 3617978-3619324 (Clockwise)

Preceding gene: 238921578

Following gene: 238921582

Centisome position: 94.9

GC content: 62.81

Gene sequence:

>1347_bases
ATGGCTCACCTCGCATCAGTCGATCAATTCATGCAGCATGTCCAGCTGCGCGATCCCCATCAGCCGGAGTTTGCACAGGC
CGTCCGTGAGGTCATGACAACCCTGTGGCCTTTTCTGCAGCAGCACCCCCACTACCGCGACAGTGCCCTGCTGGAGCGGC
TAATCGAGCCGGAGCGGGTGATCCAATTTCGTGTCAGCTGGGTGGATGACCGTAACCAGGTGCAGGTGAATCGGGCCTGG
CGCGTACAGTTCAGCTCGGCGATTGGGCCATTCAAGGGCGGAATGCGCTTTCATCCCTCGGTCAATCTGTCGATCCTCAA
GTTTCTCGGCTTTGAGCAGACCTTTAAAAACGCACTCACCACGCTGCCGATGGGCGGGGGAAAAGGTGGCAGCGATTTTA
ACCCCAAAGGGAAAAGCGCCGGTGAGATCATGCGCTTCTGTCAGGCGCTGATGACCGAGCTGTATCGCCATCTCGGCGCT
GATACCGACGTTCCGGCGGGAGATATCGGCGTCGGCGGACGCGAGGTCGGCTATATGGCGGGAATGATGAAAAAGCTCGC
CAACAACACCGCCTGTGTATTTACCGGCAAGGGGCTCTCCTTCGGCGGCAGCCTGATGCGCCCAGAGGCCACCGGCTATG
GGCTGGTCTATTTTACCGATGCCATGCTCCGGCGCCATGCCATGGGGCTGGAGGGCATGCGCATCGCCGTTTCCGGCTCT
GGAAACGTGGCGCAATACGCCATCGAGAAAGCGATGGCGCTGGGCGCCAGGGTCATTACCGCATCAGACTCCAACGGCTG
CGTAGTCGATGAGGTGGGATTTACCCCGGAAAAGCTGGCCCGCCTGTGCGCGATCAAGGCGACGCCGGATGGCCGGATCG
CCGATTACGCCCGCGAGTTTGACCTGATCTATCAGGAGGGGCGCCAGCCTTGGCACGTACCGGTCGATATCGCGCTACCC
TGCGCCACCCAGAATGAGCTGGATGCCGACGCCGCACGCACCCTGATCGCCAACGGCGTGCGCGCGGTGGCCGAAGGGGC
CAACATGCCCACCACCATTGAGGCCACCGACCTGTTCTTAGCGGCCGGCGTCCTGTTTGCCCCCGGCAAGGCGGCCAACG
CCGGTGGGGTCGCCACCTCAGGACTGGAAATGGCACAGAACGCCGCGCGCCTCGGCTGGCGCGCAGAGGAGGTCGATCGC
CGTCTGCAGCAAATCATGCTGGATATCCATCAGGCCTGCGTCGACTACAGCGGCGGTCAGAGCGGACCGGTGCACTACGT
GCGCGGCGCCAACATCGCGGGCTTCGTGAAGGTCGCCGACGCCATGCTGGCGCAGGGCGTACTGTAG

Upstream 100 bases:

>100_bases
CGTGTGAAGCGATGCTATAGTCCCAGACAGCCTATTTTTCAGGCTAATGACAGACGTCTGCGACACAGCGAATGCAATCC
AATAATAACAAGGAGTTTTT

Downstream 100 bases:

>100_bases
TCCACAGCCCTACAGCGGCAGCGACAGTCGAAAACAGGCACCCTGTCGCTGCCGCGCGATCAGAGCGATATCCCCGCCGT
GCAGCTCCAGCATCCGTTTC

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MAHLASVDQFMQHVQLRDPHQPEFAQAVREVMTTLWPFLQQHPHYRDSALLERLIEPERVIQFRVSWVDDRNQVQVNRAW
RVQFSSAIGPFKGGMRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFNPKGKSAGEIMRFCQALMTELYRHLGA
DTDVPAGDIGVGGREVGYMAGMMKKLANNTACVFTGKGLSFGGSLMRPEATGYGLVYFTDAMLRRHAMGLEGMRIAVSGS
GNVAQYAIEKAMALGARVITASDSNGCVVDEVGFTPEKLARLCAIKATPDGRIADYAREFDLIYQEGRQPWHVPVDIALP
CATQNELDADAARTLIANGVRAVAEGANMPTTIEATDLFLAAGVLFAPGKAANAGGVATSGLEMAQNAARLGWRAEEVDR
RLQQIMLDIHQACVDYSGGQSGPVHYVRGANIAGFVKVADAMLAQGVL

Sequences:

>Translated_448_residues
MAHLASVDQFMQHVQLRDPHQPEFAQAVREVMTTLWPFLQQHPHYRDSALLERLIEPERVIQFRVSWVDDRNQVQVNRAW
RVQFSSAIGPFKGGMRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFNPKGKSAGEIMRFCQALMTELYRHLGA
DTDVPAGDIGVGGREVGYMAGMMKKLANNTACVFTGKGLSFGGSLMRPEATGYGLVYFTDAMLRRHAMGLEGMRIAVSGS
GNVAQYAIEKAMALGARVITASDSNGCVVDEVGFTPEKLARLCAIKATPDGRIADYAREFDLIYQEGRQPWHVPVDIALP
CATQNELDADAARTLIANGVRAVAEGANMPTTIEATDLFLAAGVLFAPGKAANAGGVATSGLEMAQNAARLGWRAEEVDR
RLQQIMLDIHQACVDYSGGQSGPVHYVRGANIAGFVKVADAMLAQGVL
>Mature_447_residues
AHLASVDQFMQHVQLRDPHQPEFAQAVREVMTTLWPFLQQHPHYRDSALLERLIEPERVIQFRVSWVDDRNQVQVNRAWR
VQFSSAIGPFKGGMRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFNPKGKSAGEIMRFCQALMTELYRHLGAD
TDVPAGDIGVGGREVGYMAGMMKKLANNTACVFTGKGLSFGGSLMRPEATGYGLVYFTDAMLRRHAMGLEGMRIAVSGSG
NVAQYAIEKAMALGARVITASDSNGCVVDEVGFTPEKLARLCAIKATPDGRIADYAREFDLIYQEGRQPWHVPVDIALPC
ATQNELDADAARTLIANGVRAVAEGANMPTTIEATDLFLAAGVLFAPGKAANAGGVATSGLEMAQNAARLGWRAEEVDRR
LQQIMLDIHQACVDYSGGQSGPVHYVRGANIAGFVKVADAMLAQGVL

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=362, Percent_Identity=29.2817679558011, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI4885281, Length=362, Percent_Identity=28.4530386740331, Blast_Score=125, Evalue=6e-29,
Organism=Escherichia coli, GI1788059, Length=448, Percent_Identity=83.0357142857143, Blast_Score=781, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544676, Length=378, Percent_Identity=29.1005291005291, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6324951, Length=451, Percent_Identity=51.4412416851441, Blast_Score=427, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6319256, Length=453, Percent_Identity=51.2141280353201, Blast_Score=416, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24649283, Length=401, Percent_Identity=28.1795511221945, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI45553475, Length=363, Percent_Identity=28.6501377410468, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI45549226, Length=363, Percent_Identity=28.6501377410468, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24649460, Length=302, Percent_Identity=29.1390728476821, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 48476; Mature: 48345

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHLASVDQFMQHVQLRDPHQPEFAQAVREVMTTLWPFLQQHPHYRDSALLERLIEPERV
CCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHH
IQFRVSWVDDRNQVQVNRAWRVQFSSAIGPFKGGMRFHPSVNLSILKFLGFEQTFKNALT
HEEEEEEECCCCEEEEEHEEEEEEHHCCCCCCCCCEECCCCCHHHHHHHCHHHHHHHHHH
TLPMGGGKGGSDFNPKGKSAGEIMRFCQALMTELYRHLGADTDVPAGDIGVGGREVGYMA
HCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
GMMKKLANNTACVFTGKGLSFGGSLMRPEATGYGLVYFTDAMLRRHAMGLEGMRIAVSGS
HHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECC
GNVAQYAIEKAMALGARVITASDSNGCVVDEVGFTPEKLARLCAIKATPDGRIADYAREF
CCHHHHHHHHHHHHCCEEEEECCCCCEEEECCCCCHHHHHHHHEECCCCCCHHHHHHHHH
DLIYQEGRQPWHVPVDIALPCATQNELDADAARTLIANGVRAVAEGANMPTTIEATDLFL
HHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHH
AAGVLFAPGKAANAGGVATSGLEMAQNAARLGWRAEEVDRRLQQIMLDIHQACVDYSGGQ
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
SGPVHYVRGANIAGFVKVADAMLAQGVL
CCCEEEECCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AHLASVDQFMQHVQLRDPHQPEFAQAVREVMTTLWPFLQQHPHYRDSALLERLIEPERV
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHH
IQFRVSWVDDRNQVQVNRAWRVQFSSAIGPFKGGMRFHPSVNLSILKFLGFEQTFKNALT
HEEEEEEECCCCEEEEEHEEEEEEHHCCCCCCCCCEECCCCCHHHHHHHCHHHHHHHHHH
TLPMGGGKGGSDFNPKGKSAGEIMRFCQALMTELYRHLGADTDVPAGDIGVGGREVGYMA
HCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
GMMKKLANNTACVFTGKGLSFGGSLMRPEATGYGLVYFTDAMLRRHAMGLEGMRIAVSGS
HHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECC
GNVAQYAIEKAMALGARVITASDSNGCVVDEVGFTPEKLARLCAIKATPDGRIADYAREF
CCHHHHHHHHHHHHCCEEEEECCCCCEEEECCCCCHHHHHHHHEECCCCCCHHHHHHHHH
DLIYQEGRQPWHVPVDIALPCATQNELDADAARTLIANGVRAVAEGANMPTTIEATDLFL
HHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHH
AAGVLFAPGKAANAGGVATSGLEMAQNAARLGWRAEEVDRRLQQIMLDIHQACVDYSGGQ
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
SGPVHYVRGANIAGFVKVADAMLAQGVL
CCCEEEECCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308576; 6373501; 9097039; 9278503; 9298646 [H]