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The map label for this gene is greB [H]

Identifier: 238921494

GI number: 238921494

Start: 3532715

End: 3533191

Strand: Direct

Name: greB [H]

Synonym: NT01EI_3646

Alternate gene names: 238921494

Gene position: 3532715-3533191 (Clockwise)

Preceding gene: 238921491

Following gene: 238921495

Centisome position: 92.67

GC content: 54.09

Gene sequence:

>477_bases
ATGAAAACCCCGCTGATTACCCGAGAAGGTTACGAAAAGCTCAAACAGGAGCTGGATTATCTGTGGCGCGAGGAGCGTCC
GGAAGTCACCAAAAAAGTGACCTGGGCGGCGAGCCTGGGCGATCGCAGCGAAAACGCAGATTATCAGTACAACAAGAAGC
GCCTGCGCGAAATTGACAGACGCGTACGTTATCTTCGTAAGTGCATGAGTATACTTAAAATTGTCGATTACGCCCCGCAG
CAGAACGGTAAAGTCTTCTTCGGCGCCTGGGTCGAGGTGGAAAACGAGCGGGGCGATATCAAGCGCTTTCGCATCGTCGG
CTATGATGAAATCTTTGGCCGTAACGACTATATCTCCATTGACTCGCCGATGGCCCGCGCGCTGCTGAAAAAAGAGGTGG
GCGATGCCGCGACCGTGGCAACGCCGCAGGGTGACGCGGAGTGGTATGTCAATGCCATCAACTACGTCACGTCATAG

Upstream 100 bases:

>100_bases
GGGATAGGCTGTGATGTGCCGCCGCGCACTGCACCTACGCGATGGTGTGGCCGTACGGCGCGGCACTGGACGATGTGGCA
TCCACTTCAGGTTATAACAG

Downstream 100 bases:

>100_bases
TCCGCCCGCCTGCGCGGGCTGGCATTTTCCTGACTGACTCCGTATAACGGGGACTTGGCTTTTATCCATCAAGACTATCT
AACGCTATGAATGACGCATT

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MKTPLITREGYEKLKQELDYLWREERPEVTKKVTWAASLGDRSENADYQYNKKRLREIDRRVRYLRKCMSILKIVDYAPQ
QNGKVFFGAWVEVENERGDIKRFRIVGYDEIFGRNDYISIDSPMARALLKKEVGDAATVATPQGDAEWYVNAINYVTS

Sequences:

>Translated_158_residues
MKTPLITREGYEKLKQELDYLWREERPEVTKKVTWAASLGDRSENADYQYNKKRLREIDRRVRYLRKCMSILKIVDYAPQ
QNGKVFFGAWVEVENERGDIKRFRIVGYDEIFGRNDYISIDSPMARALLKKEVGDAATVATPQGDAEWYVNAINYVTS
>Mature_158_residues
MKTPLITREGYEKLKQELDYLWREERPEVTKKVTWAASLGDRSENADYQYNKKRLREIDRRVRYLRKCMSILKIVDYAPQ
QNGKVFFGAWVEVENERGDIKRFRIVGYDEIFGRNDYISIDSPMARALLKKEVGDAATVATPQGDAEWYVNAINYVTS

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=156, Percent_Identity=85.8974358974359, Blast_Score=278, Evalue=1e-76,
Organism=Escherichia coli, GI87082225, Length=158, Percent_Identity=34.8101265822785, Blast_Score=95, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 18537; Mature: 18537

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTPLITREGYEKLKQELDYLWREERPEVTKKVTWAASLGDRSENADYQYNKKRLREIDR
CCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
RVRYLRKCMSILKIVDYAPQQNGKVFFGAWVEVENERGDIKRFRIVGYDEIFGRNDYISI
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCEEEEEEECHHHHHCCCCEEEC
DSPMARALLKKEVGDAATVATPQGDAEWYVNAINYVTS
CCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKTPLITREGYEKLKQELDYLWREERPEVTKKVTWAASLGDRSENADYQYNKKRLREIDR
CCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
RVRYLRKCMSILKIVDYAPQQNGKVFFGAWVEVENERGDIKRFRIVGYDEIFGRNDYISI
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCEEEEEEECHHHHHCCCCEEEC
DSPMARALLKKEVGDAATVATPQGDAEWYVNAINYVTS
CCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]