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The map label for this gene is pckA

Identifier: 238921491

GI number: 238921491

Start: 3528561

End: 3530180

Strand: Direct

Name: pckA

Synonym: NT01EI_3643

Alternate gene names: 238921491

Gene position: 3528561-3530180 (Clockwise)

Preceding gene: 238921489

Following gene: 238921494

Centisome position: 92.56

GC content: 59.44

Gene sequence:

>1620_bases
ATGCATGCCAAAGGTTTAACCGCAGCTGATCTCGCCTCCTACGGCATTTCTGACGTTACTGAAATCGTCCACAATCCAGA
TTATGACCTCTTGTTCAAGGAAGAAACCGCCCCTGGCCTACAGGGCTATGAACGCGGTACCGTTACCTCTCTGGGCGCCG
TTGCCGTGGATACCGGTATTTTTACCGGCCGTTCCCCCAAGGATAAGTACCTGGTGCGCGACGACACCACCCGCGATACC
GTCTGGTGGTCCGATCAGGGCAAAGGCAAGAATGACAACCGCCCGCTGTCGCCGGAAGTCTGGCAGCATCTGAAGGGGCT
GGTGACCCGCCAGCTCTCCAACAAGCGCCTGTTCGTCGTCGATGCCTACTGCGGCGCCAACCCGGACAGCCGCCTGAAGG
TCCGCTTTATTACCGAGGTGGCCTGGCAGGCCCACTTCGTCAAAAATATGTTCATCCGTCCAGAGGCGGATGAACTGGCC
GGTTTTGAACCCGATTTCATCGTGATGAACGGTGCCAAATGCACCAATCCGCAGTGGCAGGCGCAGGGGCTGAACTCCGA
AAACTTTGTCGCCTTTAACCTGACCGAACGCATTCAGCTGATCGGGGGAACCTGGTACGGCGGCGAGATGAAGAAGGGGA
TGTTCTCCATCATGAACTACCTGCTGCCGTTAAAGGGCATCGCCTCCATGCACTGCTCCGCCAACGTGGGCGAACAGGGC
GACGTGGCGATCTTCTTTGGCCTCTCCGGCACCGGGAAAACCACGCTCTCAACCGATCCCAAGCGCCGCCTGATCGGCGA
TGATGAGCACGGCTGGGACGATGACGGCGTGTTTAACTTTGAGGGCGGCTGCTACGCGAAGACCATTAAACTCTCCGCCG
ACGCCGAACCCGACATCTACGGCGCCATCACCCGCGATGCGCTGCTGGAAAACGTCACGGTGCGGGCGGATGGCAGCGTC
GACTTTGACGACGGCAGCAAGACCGAAAACACCCGCGTCTCCTACCCCATCTACCACATCAAAAACATCGTGAAGCCGGT
ATCCAAAGCCGGGGCGGCCAAGAAGGTCATTTTCCTTACCGCCGACGCCTTCGGCGTCCTGCCGCCGGTCTCCCGTCTGA
CCGCGGATCAGACCCAGTACCACTTCCTGTCCGGTTTTACTGCCAAACTGGCCGGTACCGAGCGTGGCGTAACCGAACCG
ACGCCAACCTTCTCCGCCTGCTTCGGCGCCGCATTCCTGACGCTGCACCCCACCCAATACGCCGAGGTGCTGGTCCGGCG
GATGCAGGCGGCCGGAGCTCAGGCCTACCTGGTAAACACTGGCTGGAACGGCAGCGGTAAGCGGATCTCCATCAAGGATA
CCCGTGCCATTATCGATGCTATCCTCAACGGGGATATCGATCGGGTGGAAACCTTTACCCTGCCGATCTTTAATCTGGCG
GTGCCGATCGAGCTGCCCGGTGTCAATCCGGCTATCCTCGATCCGCGCGATACCTACGCCAATCGCGAGCAGTGGCAAGA
GAAAGCCCAGGATCTGGCGCAGCGTTTCATCACCAACTTTGATAAATACACCGATACGCCGGCAGGTGCTGCACTGGTTC
ACGCCGGTCCCCGGCGCTAA

Upstream 100 bases:

>100_bases
ATTCCGCTGAATTTTTGTTTTTTTGATGACTATCTCGGTTAACAAACACGTAACCACATACAGTAGACGCAGATAAAAAT
AACCTATAAGGAGTAGCTCA

Downstream 100 bases:

>100_bases
CTCACGCCCACGCGGATATACGCCACCTGACGTAAAAAAGGATGCCCAAGGGCATCCTTTTTACTGACCATCGGCTCTCT
GGCCTGGCGGAGCTTCAGAC

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MHAKGLTAADLASYGISDVTEIVHNPDYDLLFKEETAPGLQGYERGTVTSLGAVAVDTGIFTGRSPKDKYLVRDDTTRDT
VWWSDQGKGKNDNRPLSPEVWQHLKGLVTRQLSNKRLFVVDAYCGANPDSRLKVRFITEVAWQAHFVKNMFIRPEADELA
GFEPDFIVMNGAKCTNPQWQAQGLNSENFVAFNLTERIQLIGGTWYGGEMKKGMFSIMNYLLPLKGIASMHCSANVGEQG
DVAIFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDDGVFNFEGGCYAKTIKLSADAEPDIYGAITRDALLENVTVRADGSV
DFDDGSKTENTRVSYPIYHIKNIVKPVSKAGAAKKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLAGTERGVTEP
TPTFSACFGAAFLTLHPTQYAEVLVRRMQAAGAQAYLVNTGWNGSGKRISIKDTRAIIDAILNGDIDRVETFTLPIFNLA
VPIELPGVNPAILDPRDTYANREQWQEKAQDLAQRFITNFDKYTDTPAGAALVHAGPRR

Sequences:

>Translated_539_residues
MHAKGLTAADLASYGISDVTEIVHNPDYDLLFKEETAPGLQGYERGTVTSLGAVAVDTGIFTGRSPKDKYLVRDDTTRDT
VWWSDQGKGKNDNRPLSPEVWQHLKGLVTRQLSNKRLFVVDAYCGANPDSRLKVRFITEVAWQAHFVKNMFIRPEADELA
GFEPDFIVMNGAKCTNPQWQAQGLNSENFVAFNLTERIQLIGGTWYGGEMKKGMFSIMNYLLPLKGIASMHCSANVGEQG
DVAIFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDDGVFNFEGGCYAKTIKLSADAEPDIYGAITRDALLENVTVRADGSV
DFDDGSKTENTRVSYPIYHIKNIVKPVSKAGAAKKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLAGTERGVTEP
TPTFSACFGAAFLTLHPTQYAEVLVRRMQAAGAQAYLVNTGWNGSGKRISIKDTRAIIDAILNGDIDRVETFTLPIFNLA
VPIELPGVNPAILDPRDTYANREQWQEKAQDLAQRFITNFDKYTDTPAGAALVHAGPRR
>Mature_539_residues
MHAKGLTAADLASYGISDVTEIVHNPDYDLLFKEETAPGLQGYERGTVTSLGAVAVDTGIFTGRSPKDKYLVRDDTTRDT
VWWSDQGKGKNDNRPLSPEVWQHLKGLVTRQLSNKRLFVVDAYCGANPDSRLKVRFITEVAWQAHFVKNMFIRPEADELA
GFEPDFIVMNGAKCTNPQWQAQGLNSENFVAFNLTERIQLIGGTWYGGEMKKGMFSIMNYLLPLKGIASMHCSANVGEQG
DVAIFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDDGVFNFEGGCYAKTIKLSADAEPDIYGAITRDALLENVTVRADGSV
DFDDGSKTENTRVSYPIYHIKNIVKPVSKAGAAKKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLAGTERGVTEP
TPTFSACFGAAFLTLHPTQYAEVLVRRMQAAGAQAYLVNTGWNGSGKRISIKDTRAIIDAILNGDIDRVETFTLPIFNLA
VPIELPGVNPAILDPRDTYANREQWQEKAQDLAQRFITNFDKYTDTPAGAALVHAGPRR

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family

Homologues:

Organism=Escherichia coli, GI1789807, Length=534, Percent_Identity=86.3295880149813, Blast_Score=978, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322950, Length=532, Percent_Identity=45.3007518796992, Blast_Score=439, Evalue=1e-124,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PCKA_EDWI9 (C5BGS4)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002935006.1
- GeneID:   7962151
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3643
- OMA:   MFIRPTE
- ProtClustDB:   PRK09344
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00453
- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PIRSF:   PIRSF006294
- TIGRFAMs:   TIGR00224

Pfam domain/function: PF01293 PEPCK_ATP; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.49

Molecular weight: Translated: 59209; Mature: 59209

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAKGLTAADLASYGISDVTEIVHNPDYDLLFKEETAPGLQGYERGTVTSLGAVAVDTGI
CCCCCCCHHHHHHCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCHHCCHHEEECCE
FTGRSPKDKYLVRDDTTRDTVWWSDQGKGKNDNRPLSPEVWQHLKGLVTRQLSNKRLFVV
ECCCCCCCCEEEECCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEE
DAYCGANPDSRLKVRFITEVAWQAHFVKNMFIRPEADELAGFEPDFIVMNGAKCTNPQWQ
EECCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCHHHHCCCCCCEEEECCCCCCCCCHH
AQGLNSENFVAFNLTERIQLIGGTWYGGEMKKGMFSIMNYLLPLKGIASMHCSANVGEQG
CCCCCCCCEEEEEHHHEEEEEECEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DVAIFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDDGVFNFEGGCYAKTIKLSADAEPDIY
CEEEEEEECCCCCCCCCCCCHHHCCCCCCCCCCCCCEEEECCCEEEEEEEEECCCCCCHH
GAITRDALLENVTVRADGSVDFDDGSKTENTRVSYPIYHIKNIVKPVSKAGAAKKVIFLT
HHHHHHHHHHCEEEEECCCEECCCCCCCCCCEEECHHHHHHHHHHHHHHCCCCEEEEEEE
ADAFGVLPPVSRLTADQTQYHFLSGFTAKLAGTERGVTEPTPTFSACFGAAFLTLHPTQY
ECCCCCCCCHHHHCCCCHHHHHHCCCCCEECCCCCCCCCCCCCHHHHHHEEEEEECCHHH
AEVLVRRMQAAGAQAYLVNTGWNGSGKRISIKDTRAIIDAILNGDIDRVETFTLPIFNLA
HHHHHHHHHHCCCEEEEEECCCCCCCCEEEEHHHHHHHHHHHCCCHHHEEEEEEEHEEEE
VPIELPGVNPAILDPRDTYANREQWQEKAQDLAQRFITNFDKYTDTPAGAALVHAGPRR
EEEECCCCCCCEECCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCC
>Mature Secondary Structure
MHAKGLTAADLASYGISDVTEIVHNPDYDLLFKEETAPGLQGYERGTVTSLGAVAVDTGI
CCCCCCCHHHHHHCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCHHCCHHEEECCE
FTGRSPKDKYLVRDDTTRDTVWWSDQGKGKNDNRPLSPEVWQHLKGLVTRQLSNKRLFVV
ECCCCCCCCEEEECCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEE
DAYCGANPDSRLKVRFITEVAWQAHFVKNMFIRPEADELAGFEPDFIVMNGAKCTNPQWQ
EECCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCHHHHCCCCCCEEEECCCCCCCCCHH
AQGLNSENFVAFNLTERIQLIGGTWYGGEMKKGMFSIMNYLLPLKGIASMHCSANVGEQG
CCCCCCCCEEEEEHHHEEEEEECEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DVAIFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDDGVFNFEGGCYAKTIKLSADAEPDIY
CEEEEEEECCCCCCCCCCCCHHHCCCCCCCCCCCCCEEEECCCEEEEEEEEECCCCCCHH
GAITRDALLENVTVRADGSVDFDDGSKTENTRVSYPIYHIKNIVKPVSKAGAAKKVIFLT
HHHHHHHHHHCEEEEECCCEECCCCCCCCCCEEECHHHHHHHHHHHHHHCCCCEEEEEEE
ADAFGVLPPVSRLTADQTQYHFLSGFTAKLAGTERGVTEPTPTFSACFGAAFLTLHPTQY
ECCCCCCCCHHHHCCCCHHHHHHCCCCCEECCCCCCCCCCCCCHHHHHHEEEEEECCHHH
AEVLVRRMQAAGAQAYLVNTGWNGSGKRISIKDTRAIIDAILNGDIDRVETFTLPIFNLA
HHHHHHHHHHCCCEEEEEECCCCCCCCEEEEHHHHHHHHHHHCCCHHHEEEEEEEHEEEE
VPIELPGVNPAILDPRDTYANREQWQEKAQDLAQRFITNFDKYTDTPAGAALVHAGPRR
EEEECCCCCCCEECCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA