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The map label for this gene is iscA

Identifier: 238921051

GI number: 238921051

Start: 3061908

End: 3062231

Strand: Reverse

Name: iscA

Synonym: NT01EI_3181

Alternate gene names: 238921051

Gene position: 3062231-3061908 (Counterclockwise)

Preceding gene: 238921052

Following gene: 238921050

Centisome position: 80.32

GC content: 49.69

Gene sequence:

>324_bases
ATGTCGATTACCATGAGCGACTCCGCTGCAAAGCGCGTACAGACATTTTTAACCAATCGCGGTAAGGGATTGGGCCTGCG
TCTGGGGGTAAAAACCTCGGGTTGTTCAGGCATGGCGTATGTGCTGGAGTTTGTCGATGTCCTGAACGAGGACGATGTGG
TTTTCGAGGATCAAGGAGTGAAGGTTATCGTTGACGGAAAGAGCCTGGTGTACCTCGATGGTACCGAGTTGGACTTCGTT
AAAGAGGGGCTTAACGAAGGCTTTAAGTTCAACAACCCAAATGTTAAGAATGAGTGCGGCTGCGGCGAGAGCTTTAACGT
TTAA

Upstream 100 bases:

>100_bases
CGCCGATTATAAAAACAAGCATAGCGCGGAATAAGCGCCGTGCTCCGGTGGCCGGCTATCCGGTCACCGTCACCCTGAAT
TTTTTGACTGAGGTTGTTGT

Downstream 100 bases:

>100_bases
CCCGCGCCCACGTCACCGGCGGGCGTCGCCTCTCCTTAATTAGCTGACGTCGGCGACGCCGCTTTTACGGTATTAGCATC
AGAGTACGGCATGGACTATT

Product: iron-sulfur cluster assembly protein IscA

Products: NA

Alternate protein names: Iron-sulfur cluster assembly protein

Number of amino acids: Translated: 107; Mature: 106

Protein sequence:

>107_residues
MSITMSDSAAKRVQTFLTNRGKGLGLRLGVKTSGCSGMAYVLEFVDVLNEDDVVFEDQGVKVIVDGKSLVYLDGTELDFV
KEGLNEGFKFNNPNVKNECGCGESFNV

Sequences:

>Translated_107_residues
MSITMSDSAAKRVQTFLTNRGKGLGLRLGVKTSGCSGMAYVLEFVDVLNEDDVVFEDQGVKVIVDGKSLVYLDGTELDFV
KEGLNEGFKFNNPNVKNECGCGESFNV
>Mature_106_residues
SITMSDSAAKRVQTFLTNRGKGLGLRLGVKTSGCSGMAYVLEFVDVLNEDDVVFEDQGVKVIVDGKSLVYLDGTELDFVK
EGLNEGFKFNNPNVKNECGCGESFNV

Specific function: Is able to transfer iron-sulfur clusters to apo- ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that iscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transi

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesB/iscA family

Homologues:

Organism=Homo sapiens, GI52426768, Length=106, Percent_Identity=38.6792452830189, Blast_Score=98, Evalue=1e-21,
Organism=Homo sapiens, GI160420328, Length=83, Percent_Identity=42.1686746987952, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788877, Length=107, Percent_Identity=81.3084112149533, Blast_Score=181, Evalue=9e-48,
Organism=Escherichia coli, GI1787974, Length=105, Percent_Identity=46.6666666666667, Blast_Score=113, Evalue=3e-27,
Organism=Escherichia coli, GI1786351, Length=110, Percent_Identity=40.9090909090909, Blast_Score=79, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI25146411, Length=106, Percent_Identity=35.8490566037736, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI193209025, Length=106, Percent_Identity=35.8490566037736, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71998452, Length=106, Percent_Identity=34.9056603773585, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323001, Length=105, Percent_Identity=39.0476190476191, Blast_Score=86, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24642263, Length=107, Percent_Identity=42.0560747663551, Blast_Score=96, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24649697, Length=108, Percent_Identity=35.1851851851852, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISCA_EDWI9 (C5BEU3)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934566.1
- ProteinModelPortal:   C5BEU3
- GeneID:   7958699
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3181
- OMA:   ELAPEDQ
- ProtClustDB:   CLSK2813571
- HAMAP:   MF_01429
- InterPro:   IPR000361
- InterPro:   IPR016092
- InterPro:   IPR017870
- InterPro:   IPR011302
- Gene3D:   G3DSA:2.60.300.12
- PANTHER:   PTHR10072
- TIGRFAMs:   TIGR00049
- TIGRFAMs:   TIGR02011

Pfam domain/function: PF01521 Fe-S_biosyn; SSF89360 HesB_yadR_yfhF-like

EC number: NA

Molecular weight: Translated: 11607; Mature: 11476

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS01152 HESB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITMSDSAAKRVQTFLTNRGKGLGLRLGVKTSGCSGMAYVLEFVDVLNEDDVVFEDQGV
CEEECCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCEEECCCCC
KVIVDGKSLVYLDGTELDFVKEGLNEGFKFNNPNVKNECGCGESFNV
EEEECCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SITMSDSAAKRVQTFLTNRGKGLGLRLGVKTSGCSGMAYVLEFVDVLNEDDVVFEDQGV
EEECCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCEEECCCCC
KVIVDGKSLVYLDGTELDFVKEGLNEGFKFNNPNVKNECGCGESFNV
EEEECCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA