Click here to switch to the map view.

The map label for this gene is nifU [H]

Identifier: 238921052

GI number: 238921052

Start: 3062298

End: 3062684

Strand: Reverse

Name: nifU [H]

Synonym: NT01EI_3182

Alternate gene names: 238921052

Gene position: 3062684-3062298 (Counterclockwise)

Preceding gene: 238921053

Following gene: 238921051

Centisome position: 80.34

GC content: 55.04

Gene sequence:

>387_bases
ATGGCTTACAGCGATAAAGTGATCGATCACTATGAGAATCCGCGCAACGTCGGATCCTTTGACAATAGCGATCCCAACGT
CGGTAGCGGTATGGTCGGCGCCCCGGCCTGCGGTGACGTGATGAAGTTGCAGATTAAAGTCAACAATGAGGGTATCATTG
AGGACGCCCGTTTCAAGACCTACGGCTGTGGCTCTGCCATTGCCTCCAGCTCGCTGGTCACTGAGTGGGTCAAGGGTAAG
TCCCTGGAGCAGGCGGCGGAGATCAAGAATACCGACATCGCCGAGGAGCTGGCGCTGCCGCCGGTGAAGATCCACTGCTC
GATCTTGGCAGAAGACGCCATTAAGGCAGCGATCGCCGATTATAAAAACAAGCATAGCGCGGAATAA

Upstream 100 bases:

>100_bases
TCTCACCGCTGTGGGAGATGCATAAGCAGGGCGTGGATATCAGCACCATCGAATGGTCAGCCCATTAATTCCCAGGACAC
CCTTTTAGGAGCATCGATAA

Downstream 100 bases:

>100_bases
GCGCCGTGCTCCGGTGGCCGGCTATCCGGTCACCGTCACCCTGAATTTTTTGACTGAGGTTGTTGTATGTCGATTACCAT
GAGCGACTCCGCTGCAAAGC

Product: FeS cluster assembly scaffold IscU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 128; Mature: 127

Protein sequence:

>128_residues
MAYSDKVIDHYENPRNVGSFDNSDPNVGSGMVGAPACGDVMKLQIKVNNEGIIEDARFKTYGCGSAIASSSLVTEWVKGK
SLEQAAEIKNTDIAEELALPPVKIHCSILAEDAIKAAIADYKNKHSAE

Sequences:

>Translated_128_residues
MAYSDKVIDHYENPRNVGSFDNSDPNVGSGMVGAPACGDVMKLQIKVNNEGIIEDARFKTYGCGSAIASSSLVTEWVKGK
SLEQAAEIKNTDIAEELALPPVKIHCSILAEDAIKAAIADYKNKHSAE
>Mature_127_residues
AYSDKVIDHYENPRNVGSFDNSDPNVGSGMVGAPACGDVMKLQIKVNNEGIIEDARFKTYGCGSAIASSSLVTEWVKGKS
LEQAAEIKNTDIAEELALPPVKIHCSILAEDAIKAAIADYKNKHSAE

Specific function: May be involved in the formation or repair of [Fe-S] clusters present in iron-sulfur proteins (Potential) [H]

COG id: COG0822

COG function: function code C; NifU homolog involved in Fe-S cluster formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifU family [H]

Homologues:

Organism=Homo sapiens, GI56699456, Length=123, Percent_Identity=75.609756097561, Blast_Score=203, Evalue=3e-53,
Organism=Homo sapiens, GI24307953, Length=123, Percent_Identity=74.7967479674797, Blast_Score=200, Evalue=3e-52,
Organism=Escherichia coli, GI1788878, Length=128, Percent_Identity=89.84375, Blast_Score=237, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI17543474, Length=124, Percent_Identity=67.741935483871, Blast_Score=191, Evalue=9e-50,
Organism=Saccharomyces cerevisiae, GI6325122, Length=124, Percent_Identity=70.9677419354839, Blast_Score=190, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6324800, Length=123, Percent_Identity=72.3577235772358, Blast_Score=187, Evalue=4e-49,
Organism=Drosophila melanogaster, GI21355597, Length=123, Percent_Identity=73.1707317073171, Blast_Score=197, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011339
- InterPro:   IPR002871 [H]

Pfam domain/function: PF01592 NifU_N [H]

EC number: NA

Molecular weight: Translated: 13697; Mature: 13566

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYSDKVIDHYENPRNVGSFDNSDPNVGSGMVGAPACGDVMKLQIKVNNEGIIEDARFKT
CCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEECCCCCC
YGCGSAIASSSLVTEWVKGKSLEQAAEIKNTDIAEELALPPVKIHCSILAEDAIKAAIAD
CCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHCCCCEEEHHHHHHHHHHHHHHHH
YKNKHSAE
HCCCCCCC
>Mature Secondary Structure 
AYSDKVIDHYENPRNVGSFDNSDPNVGSGMVGAPACGDVMKLQIKVNNEGIIEDARFKT
CCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEECCCCCC
YGCGSAIASSSLVTEWVKGKSLEQAAEIKNTDIAEELALPPVKIHCSILAEDAIKAAIAD
CCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHCCCCEEEHHHHHHHHHHHHHHHH
YKNKHSAE
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]