Click here to switch to the map view.

The map label for this gene is ispG [H]

Identifier: 238921039

GI number: 238921039

Start: 3051338

End: 3052465

Strand: Reverse

Name: ispG [H]

Synonym: NT01EI_3169

Alternate gene names: 238921039

Gene position: 3052465-3051338 (Counterclockwise)

Preceding gene: 238921040

Following gene: 238921038

Centisome position: 80.07

GC content: 59.04

Gene sequence:

>1128_bases
ATGCATAGTACTCCATCCCCCATTAAGCGCCGTAAATCGACGCGTATCCATGTCGGCAGCGTGCCGATCGGTGACGGCGC
ACCCATCACCGTCCAGTCCATGACCAACACCCGCACCACCGACGTCGATGCGACAGTGGCGCAGATCCGTGCACTGGAGC
GGGTCGGAGTGGATATTGTGCGCGTATCGGTGCCCACCATGGAGGCGGCCGAAGCATTTCGTTTGATCAAACAGCAGGCG
CAGGTACCGTTAGTAGCCGATATTCACTTTGACTACCGTATTGCGCTGAAGGTTGCGGAGTATGGCGTAGACTGCCTGCG
TATCAATCCGGGCAATATCGGTAATGAGAGCCGGATCCGCTCGGTGGTTGACTGCGCGCGCGATCGCGGTATTCCGATCC
GCATCGGTATTAACGCCGGATCACTGGAGAAGGACATCCAGGAGAAGTACGGCGAGCCGACGCCGCAGGCGTTGTTGGAG
TCGGCCATGCGCCATGTCGATATTCTGGATCGCCTCAACTTTGACCAGTTTAAGGTGAGCGTGAAGGCTTCCGACGTGTT
CCTGGCCGTTGACGCCTACCGTCTGCTGGCGGCGCGTATCGATCAACCGCTGCATTTGGGGATCACTGAGGCCGGGGGTG
CCCGCAGTGGCTCGGTGAAGTCTGCTGTCGGGCTGGGGATGCTGCTGGCGGAGGGGATCGGTGATACCCTGCGCGTATCG
CTGGCCGCCGATCCGGTAGAAGAAGTGAAGGTGGGATTTGATATCCTCAAGTCGCTGCGCATCCGCTCGCGCGGGATCAA
CTTTATCGCCTGTCCCACCTGCTCCCGTCAGGAGTTTGATGTGATCGGAACGGTGAATGCACTGGAACAGCGCCTGGAAG
ACATCATCACGCCGATGGACGTATCGATCATCGGCTGCGTGGTCAATGGACCCGGCGAAGCGCTGGTCTCAACCTTGGGC
GTGACCGGCGGACACAATAAGAGCGGCTTCTATGAGGACGGCATCCGTCAGAAGGAGCGCTTTGATAACAGTGCGATCAT
CGATCTACTGGAGGCGAAGATCCGCGCTAAGGCCGCCATGCTGGATGAAAAGATGCGCATTGGCGTCACGACGCTGGACG
ATCGGTGA

Upstream 100 bases:

>100_bases
GATCTGAGTAGTTTCATCAAGTCAAGTCAGGTTGCCCGTTTTACGTTAGACGCGCAGTCTGCGGCGGCTAACTGAGCTAT
AGCCAGTGCGGAGAGAAGTC

Downstream 100 bases:

>100_bases
TGGCGGGCGGCGCGTTCTGGCGCCGCCGCTGTTTGCCCGCGTTGAAGAGGGGGCGTGAATCCCTCTATAATCGGGCCAGT
TTTTGCAAATTACATAGAGA

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MHSTPSPIKRRKSTRIHVGSVPIGDGAPITVQSMTNTRTTDVDATVAQIRALERVGVDIVRVSVPTMEAAEAFRLIKQQA
QVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNESRIRSVVDCARDRGIPIRIGINAGSLEKDIQEKYGEPTPQALLE
SAMRHVDILDRLNFDQFKVSVKASDVFLAVDAYRLLAARIDQPLHLGITEAGGARSGSVKSAVGLGMLLAEGIGDTLRVS
LAADPVEEVKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLG
VTGGHNKSGFYEDGIRQKERFDNSAIIDLLEAKIRAKAAMLDEKMRIGVTTLDDR

Sequences:

>Translated_375_residues
MHSTPSPIKRRKSTRIHVGSVPIGDGAPITVQSMTNTRTTDVDATVAQIRALERVGVDIVRVSVPTMEAAEAFRLIKQQA
QVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNESRIRSVVDCARDRGIPIRIGINAGSLEKDIQEKYGEPTPQALLE
SAMRHVDILDRLNFDQFKVSVKASDVFLAVDAYRLLAARIDQPLHLGITEAGGARSGSVKSAVGLGMLLAEGIGDTLRVS
LAADPVEEVKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLG
VTGGHNKSGFYEDGIRQKERFDNSAIIDLLEAKIRAKAAMLDEKMRIGVTTLDDR
>Mature_375_residues
MHSTPSPIKRRKSTRIHVGSVPIGDGAPITVQSMTNTRTTDVDATVAQIRALERVGVDIVRVSVPTMEAAEAFRLIKQQA
QVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNESRIRSVVDCARDRGIPIRIGINAGSLEKDIQEKYGEPTPQALLE
SAMRHVDILDRLNFDQFKVSVKASDVFLAVDAYRLLAARIDQPLHLGITEAGGARSGSVKSAVGLGMLLAEGIGDTLRVS
LAADPVEEVKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLG
VTGGHNKSGFYEDGIRQKERFDNSAIIDLLEAKIRAKAAMLDEKMRIGVTTLDDR

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=373, Percent_Identity=85.7908847184987, Blast_Score=651, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 40704; Mature: 40704

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSTPSPIKRRKSTRIHVGSVPIGDGAPITVQSMTNTRTTDVDATVAQIRALERVGVDIV
CCCCCCHHHHCCCCEEEECCEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEE
RVSVPTMEAAEAFRLIKQQAQVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNESRIR
EEECCCHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEHHCCCEEEEECCCCCCCHHHHH
SVVDCARDRGIPIRIGINAGSLEKDIQEKYGEPTPQALLESAMRHVDILDRLNFDQFKVS
HHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEE
VKASDVFLAVDAYRLLAARIDQPLHLGITEAGGARSGSVKSAVGLGMLLAEGIGDTLRVS
EEECEEEEEHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEE
LAADPVEEVKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMD
EECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEECHHHHHHHHHHHCCCCC
VSIIGCVVNGPGEALVSTLGVTGGHNKSGFYEDGIRQKERFDNSAIIDLLEAKIRAKAAM
EEEEEEEECCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LDEKMRIGVTTLDDR
HHHHHHCCCEECCCC
>Mature Secondary Structure
MHSTPSPIKRRKSTRIHVGSVPIGDGAPITVQSMTNTRTTDVDATVAQIRALERVGVDIV
CCCCCCHHHHCCCCEEEECCEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEE
RVSVPTMEAAEAFRLIKQQAQVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNESRIR
EEECCCHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEHHCCCEEEEECCCCCCCHHHHH
SVVDCARDRGIPIRIGINAGSLEKDIQEKYGEPTPQALLESAMRHVDILDRLNFDQFKVS
HHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEE
VKASDVFLAVDAYRLLAARIDQPLHLGITEAGGARSGSVKSAVGLGMLLAEGIGDTLRVS
EEECEEEEEHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEE
LAADPVEEVKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMD
EECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEECHHHHHHHHHHHCCCCC
VSIIGCVVNGPGEALVSTLGVTGGHNKSGFYEDGIRQKERFDNSAIIDLLEAKIRAKAAM
EEEEEEEECCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LDEKMRIGVTTLDDR
HHHHHHCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA