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The map label for this gene is hisS

Identifier: 238921038

GI number: 238921038

Start: 3049957

End: 3051231

Strand: Reverse

Name: hisS

Synonym: NT01EI_3168

Alternate gene names: 238921038

Gene position: 3051231-3049957 (Counterclockwise)

Preceding gene: 238921039

Following gene: 238921037

Centisome position: 80.04

GC content: 61.1

Gene sequence:

>1275_bases
GTGGCTAAGAATATCCAGGCCATTCGTGGCATGAACGACTATTTGCCGGCAGATACGGCGCTGTGGCAGCCGATTGAAGC
GGCGCTTAAGCAGGTGCTGGCCAGCTACGGCTACAGCGAAATCCGTTTGCCGATTGTAGAGCAGACCCCGTTATTCAAAC
GCGCCATCGGTGAAGTGACCGATGTCGTTGAAAAAGAAATGTATACCTTCGAGGATCGCAATGGCGACAGCCTGACCCTG
CGTCCGGAAGGCACCGCTGGCTGCGTGCGCGCCGGTATCGAACATGGTCTGCTGTACAATCAGGAGCAGCGTTTGTGGTA
CATCGGGCCGATGTTCCGCCACGAACGTCCGCAGAAGGGGCGCTATCGTCAGTTTCATCAGTTGGGGGCTGAGGTGTTTG
GCCTGGCCGGTCCCGATATCGATGCGGAAATCATCATGATGACCGCCCGCTGGTGGAAAGTGCTGGGGATTGCCGATCAC
GTCACACTGGAGCTGAACTCCATCGGCTCACTGGAGGCGCGTGCCGCCTACCGCGACGCCCTGGTTGCCTACCTGGAGCA
GTATAAGGACCAGCTGGATGAAGATTGCCTGCGCCGTATGTATAGCAATCCGCTGCGCGTGCTGGACTCGAAGAATCCAC
AGGTACAGGCGCTGCTGAACGCGGCTCCGCGCCTGTCCGAGTATCTGGACGCCGACTCCCGCGCACACTTTGACGGCCTG
TGCCGTCTGCTGGACGACGCCGGGATCGTCTATACGGTCAACGAGCGTCTGGTGCGCGGGCTGGATTACTATAACCGCAC
TGTGTTTGAGTGGGTTACCACCAGCCTTGGTGCTCAGGGCACCGTCTGCGCAGGAGGCCGTTACGATGGCCTGGTGGCGC
AACTGGGCGGTCATGCGACGTCGGCGGTGGGTTTTGCCATGGGGCTGGAGCGTCTGGTGCTTTTAGTGCAGGCAGTTAAT
CCGGCCTTCCTGCCGCAGCGCGCGGTCGATGTCTACCTGATCGCCGCCGGCGAGGGCACCCAAAGCGCGGCGATGCGTCT
GGCGGAGCAGCTGCGCGACGCCCTGCCGACGCTGCGTCTGATGACCAACTACGGCGGCGGTAACTTCAAGAAGCAGTTTG
CCCGTGCCGATAAATGGGGCGCCCGCATTGCGCTGGTGTTGGGCGAGAGCGAAGTACAGAGTGGTCAGGTGAACGTGAAG
TGCCTGGCAAGCGGTGAACAGCAGACCGTGGCGCAGGATCAGGCCGCGACGCTGCTGGCAACCCTGTTGGGCTAA

Upstream 100 bases:

>100_bases
GCGGCGCGTTCTGGCGCCGCCGCTGTTTGCCCGCGTTGAAGAGGGGGCGTGAATCCCTCTATAATCGGGCCAGTTTTTGC
AAATTACATAGAGAGTTGAC

Downstream 100 bases:

>100_bases
GGAGCAGGAAACAGTGGAAGTCTATAGCACAGAGAACGAACAGGTTGATGCCATCCGCCATTTCTTCCAGGAGTATGGCA
AGACGCTGGTGGTGGGCGTG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MAKNIQAIRGMNDYLPADTALWQPIEAALKQVLASYGYSEIRLPIVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTL
RPEGTAGCVRAGIEHGLLYNQEQRLWYIGPMFRHERPQKGRYRQFHQLGAEVFGLAGPDIDAEIIMMTARWWKVLGIADH
VTLELNSIGSLEARAAYRDALVAYLEQYKDQLDEDCLRRMYSNPLRVLDSKNPQVQALLNAAPRLSEYLDADSRAHFDGL
CRLLDDAGIVYTVNERLVRGLDYYNRTVFEWVTTSLGAQGTVCAGGRYDGLVAQLGGHATSAVGFAMGLERLVLLVQAVN
PAFLPQRAVDVYLIAAGEGTQSAAMRLAEQLRDALPTLRLMTNYGGGNFKKQFARADKWGARIALVLGESEVQSGQVNVK
CLASGEQQTVAQDQAATLLATLLG

Sequences:

>Translated_424_residues
MAKNIQAIRGMNDYLPADTALWQPIEAALKQVLASYGYSEIRLPIVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTL
RPEGTAGCVRAGIEHGLLYNQEQRLWYIGPMFRHERPQKGRYRQFHQLGAEVFGLAGPDIDAEIIMMTARWWKVLGIADH
VTLELNSIGSLEARAAYRDALVAYLEQYKDQLDEDCLRRMYSNPLRVLDSKNPQVQALLNAAPRLSEYLDADSRAHFDGL
CRLLDDAGIVYTVNERLVRGLDYYNRTVFEWVTTSLGAQGTVCAGGRYDGLVAQLGGHATSAVGFAMGLERLVLLVQAVN
PAFLPQRAVDVYLIAAGEGTQSAAMRLAEQLRDALPTLRLMTNYGGGNFKKQFARADKWGARIALVLGESEVQSGQVNVK
CLASGEQQTVAQDQAATLLATLLG
>Mature_423_residues
AKNIQAIRGMNDYLPADTALWQPIEAALKQVLASYGYSEIRLPIVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLR
PEGTAGCVRAGIEHGLLYNQEQRLWYIGPMFRHERPQKGRYRQFHQLGAEVFGLAGPDIDAEIIMMTARWWKVLGIADHV
TLELNSIGSLEARAAYRDALVAYLEQYKDQLDEDCLRRMYSNPLRVLDSKNPQVQALLNAAPRLSEYLDADSRAHFDGLC
RLLDDAGIVYTVNERLVRGLDYYNRTVFEWVTTSLGAQGTVCAGGRYDGLVAQLGGHATSAVGFAMGLERLVLLVQAVNP
AFLPQRAVDVYLIAAGEGTQSAAMRLAEQLRDALPTLRLMTNYGGGNFKKQFARADKWGARIALVLGESEVQSGQVNVKC
LASGEQQTVAQDQAATLLATLLG

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1788861, Length=424, Percent_Identity=82.0754716981132, Blast_Score=724, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71993686, Length=470, Percent_Identity=24.6808510638298, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71993693, Length=470, Percent_Identity=24.6808510638298, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_EDWI9 (C5BET0)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934553.1
- GeneID:   7958686
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3168
- OMA:   VFEWVTT
- ProtClustDB:   PRK00037
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 46905; Mature: 46774

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNIQAIRGMNDYLPADTALWQPIEAALKQVLASYGYSEIRLPIVEQTPLFKRAIGEVT
CCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHH
DVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQEQRLWYIGPMFRHERPQKG
HHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHCCEECCCCCEEEECCHHHCCCCCCC
RYRQFHQLGAEVFGLAGPDIDAEIIMMTARWWKVLGIADHVTLELNSIGSLEARAAYRDA
HHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHEECCEEEEEECCCCCHHHHHHHHHH
LVAYLEQYKDQLDEDCLRRMYSNPLRVLDSKNPQVQALLNAAPRLSEYLDADSRAHFDGL
HHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
CRLLDDAGIVYTVNERLVRGLDYYNRTVFEWVTTSLGAQGTVCAGGRYDGLVAQLGGHAT
HHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHCCCHH
SAVGFAMGLERLVLLVQAVNPAFLPQRAVDVYLIAAGEGTQSAAMRLAEQLRDALPTLRL
HHHHHHHHHHHHHHHHHHCCCHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
MTNYGGGNFKKQFARADKWGARIALVLGESEVQSGQVNVKCLASGEQQTVAQDQAATLLA
HHCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHH
TLLG
HHHC
>Mature Secondary Structure 
AKNIQAIRGMNDYLPADTALWQPIEAALKQVLASYGYSEIRLPIVEQTPLFKRAIGEVT
CCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHH
DVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQEQRLWYIGPMFRHERPQKG
HHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHCCEECCCCCEEEECCHHHCCCCCCC
RYRQFHQLGAEVFGLAGPDIDAEIIMMTARWWKVLGIADHVTLELNSIGSLEARAAYRDA
HHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHEECCEEEEEECCCCCHHHHHHHHHH
LVAYLEQYKDQLDEDCLRRMYSNPLRVLDSKNPQVQALLNAAPRLSEYLDADSRAHFDGL
HHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
CRLLDDAGIVYTVNERLVRGLDYYNRTVFEWVTTSLGAQGTVCAGGRYDGLVAQLGGHAT
HHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHCCCHH
SAVGFAMGLERLVLLVQAVNPAFLPQRAVDVYLIAAGEGTQSAAMRLAEQLRDALPTLRL
HHHHHHHHHHHHHHHHHHCCCHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
MTNYGGGNFKKQFARADKWGARIALVLGESEVQSGQVNVKCLASGEQQTVAQDQAATLLA
HHCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHH
TLLG
HHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA