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The map label for this gene is mlaA [H]

Identifier: 238920648

GI number: 238920648

Start: 2659261

End: 2660028

Strand: Reverse

Name: mlaA [H]

Synonym: NT01EI_2760

Alternate gene names: 238920648

Gene position: 2660028-2659261 (Counterclockwise)

Preceding gene: 238920649

Following gene: 238920646

Centisome position: 69.77

GC content: 57.42

Gene sequence:

>768_bases
ATGAAATCTCGTCTGGCGGGGGTGGCCCTGGCAAGCGTGGTACTGGCCGGTTGTGCCACGGGATCTTCCCGGCAGGATAC
ACAGGGGCGATCGGATCCGCTGGAGGGCTTTAACCGGGCCATGTTCAACGTTAACTATGATTACCTTGACCCCTATTTGG
TACGGCCGGTTGCTGTGGTATGGCGTGATTATGTTCCCAGCCCGGCGCGTAATGGTCTGATGAATTTCTTCGTTAACCTG
GCGGAGCCAGCCAGCATGGTGAACTGTTTTGCCGAAGGGCGGATTTATGATGGCTTTCGCCACCTTAACCGCTTTTTCCT
CAACACGACGCTGGGGATAGGGGGGCTGATTGACGTTGCGGCGATGGCTAACCCTGATAAGCTGAGCAAACAGGATCCGC
AGCGCTTCGGCAGTACGCTGGGCTACTACCACATTCCCTATGGGCCCTACCTGGTGCTGCCGGGCTACGGTAGCTTTACC
CTGCGTCAGGAGGGGGGGAATTCGGTGGATTACCTCTACCCACCGCTGGGATGGCTGACCTTCTGGATGTCGGCCGGTAA
GTGGGCGCTGGAAGGTATTGAAACCCGGGCTCAGCTGCTGGACTCCGATGGTCTGCTGCGTAACTCGCCCGATCCGTACA
TCACGGTGCGCGAGGCTTATTTCCAGTACAATGACTTTCTGGTCAACGGCGGCCAGGTTCAGCCAGAGGCCAATCCCAAT
GCCCAGGCGATCCAGGATGATTTGAAAGATATCGACTCGGCCAACTGA

Upstream 100 bases:

>100_bases
GCGATAACCCTTAGCCGTCAGGTACCTTAAGCGGCTGGGCGAAGTGTAAGGCTGTTGAAGGGCGCCCCGTTGAGGGGCGC
TCTGGTTTATGGAGCAAATT

Downstream 100 bases:

>100_bases
GGTATTTGGCGACGTGGGTGCCCGACGAGCACCCGCTTTTTAGCACGTTGCTGACGACATTCGTATCTCCCCTTCTGTGG
CCTCTCCCGCGTCTGCGTCC

Product: lipoprotein VacJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKSRLAGVALASVVLAGCATGSSRQDTQGRSDPLEGFNRAMFNVNYDYLDPYLVRPVAVVWRDYVPSPARNGLMNFFVNL
AEPASMVNCFAEGRIYDGFRHLNRFFLNTTLGIGGLIDVAAMANPDKLSKQDPQRFGSTLGYYHIPYGPYLVLPGYGSFT
LRQEGGNSVDYLYPPLGWLTFWMSAGKWALEGIETRAQLLDSDGLLRNSPDPYITVREAYFQYNDFLVNGGQVQPEANPN
AQAIQDDLKDIDSAN

Sequences:

>Translated_255_residues
MKSRLAGVALASVVLAGCATGSSRQDTQGRSDPLEGFNRAMFNVNYDYLDPYLVRPVAVVWRDYVPSPARNGLMNFFVNL
AEPASMVNCFAEGRIYDGFRHLNRFFLNTTLGIGGLIDVAAMANPDKLSKQDPQRFGSTLGYYHIPYGPYLVLPGYGSFT
LRQEGGNSVDYLYPPLGWLTFWMSAGKWALEGIETRAQLLDSDGLLRNSPDPYITVREAYFQYNDFLVNGGQVQPEANPN
AQAIQDDLKDIDSAN
>Mature_255_residues
MKSRLAGVALASVVLAGCATGSSRQDTQGRSDPLEGFNRAMFNVNYDYLDPYLVRPVAVVWRDYVPSPARNGLMNFFVNL
AEPASMVNCFAEGRIYDGFRHLNRFFLNTTLGIGGLIDVAAMANPDKLSKQDPQRFGSTLGYYHIPYGPYLVLPGYGSFT
LRQEGGNSVDYLYPPLGWLTFWMSAGKWALEGIETRAQLLDSDGLLRNSPDPYITVREAYFQYNDFLVNGGQVQPEANPN
AQAIQDDLKDIDSAN

Specific function: Actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane [H]

COG id: COG2853

COG function: function code M; Surface lipoprotein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaA family [H]

Homologues:

Organism=Escherichia coli, GI1788688, Length=253, Percent_Identity=68.3794466403162, Blast_Score=350, Evalue=7e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007428 [H]

Pfam domain/function: PF04333 VacJ [H]

EC number: NA

Molecular weight: Translated: 28275; Mature: 28275

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSRLAGVALASVVLAGCATGSSRQDTQGRSDPLEGFNRAMFNVNYDYLDPYLVRPVAVV
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHEECCHHHCCHHHHHHHHHH
WRDYVPSPARNGLMNFFVNLAEPASMVNCFAEGRIYDGFRHLNRFFLNTTLGIGGLIDVA
HHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
AMANPDKLSKQDPQRFGSTLGYYHIPYGPYLVLPGYGSFTLRQEGGNSVDYLYPPLGWLT
HCCCCHHCCCCCHHHHHCCCCEEECCCCCEEEECCCCCEEEECCCCCCCCEEECCHHHHH
FWMSAGKWALEGIETRAQLLDSDGLLRNSPDPYITVREAYFQYNDFLVNGGQVQPEANPN
HHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHCCEEEECCEECCCCCCC
AQAIQDDLKDIDSAN
HHHHHHHHHHCCCCC
>Mature Secondary Structure
MKSRLAGVALASVVLAGCATGSSRQDTQGRSDPLEGFNRAMFNVNYDYLDPYLVRPVAVV
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHEECCHHHCCHHHHHHHHHH
WRDYVPSPARNGLMNFFVNLAEPASMVNCFAEGRIYDGFRHLNRFFLNTTLGIGGLIDVA
HHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
AMANPDKLSKQDPQRFGSTLGYYHIPYGPYLVLPGYGSFTLRQEGGNSVDYLYPPLGWLT
HCCCCHHCCCCCHHHHHCCCCEEECCCCCEEEECCCCCEEEECCCCCCCCEEECCHHHHH
FWMSAGKWALEGIETRAQLLDSDGLLRNSPDPYITVREAYFQYNDFLVNGGQVQPEANPN
HHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHCCEEEECCEECCCCCCC
AQAIQDDLKDIDSAN
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]