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The map label for this gene is purF [H]

Identifier: 238920602

GI number: 238920602

Start: 2606988

End: 2608505

Strand: Reverse

Name: purF [H]

Synonym: NT01EI_2714

Alternate gene names: 238920602

Gene position: 2608505-2606988 (Counterclockwise)

Preceding gene: 238920603

Following gene: 238920601

Centisome position: 68.42

GC content: 58.43

Gene sequence:

>1518_bases
ATGTGCGGTATTGTCGGTATCGCCGGTTTTACACCGGTAAACCAGTCTATCTATGATGCTCTGACGGTGTTGCAGCACCG
CGGCCAGGACGCCGCTGGCATCGTCACCATCGATGATAACCATGCCTTCCGCCTGCGTAAGGCCAATGGACTGGTCAGTG
ATGTGTTTGAGGCGCGCCATATGCAGCGCCTGCAGGGAAATATGGGGATCGGGCACGTGCGCTATCCCACCGCCGGAAGC
TCCAGCGCATCAGAGGCTCAGCCGTTCTATGTTAACTCCCCCTTTGGCATCACCCTGGCGCACAACGGCAATCTGACTAA
TGCCCATCAGCTGAAGCAGCACCTGTTTGAGCATGCCCGCCGCCATGTCAATACCACCTCAGATTCCGAGATCCTGCTGA
ATATCTTCGCCTACGAGCTGGATCGCTACGATCACTATCCGCTGAGTGACGAAAATATCTTCGCCGCCGTCGCGGCGACC
CACCGCCAAATCCGCGGGGCTTATGCCTGCGTGGCGATGATCATCGGCCATGGCATGGTGGCGTTCCGCGACCCGAACGG
TATCCGGCCGCTGGTGATCGGCAAGCGAACCCTAGAGGATGGCCGTACAGAATATATGGTCGCCTCAGAGAGCGTGGCGC
TGGATACGCTGGGGTTCGACTTTATCCGTGACGTGGCGCCGGGCGAGGCGATTTACGTTAATCTGCAGGGACAGCTGTTT
ACCCGCCAGTGCGCCCAGGATCCGAAGAGTCATCCCTGCCTGTTTGAGTATGTCTATTTTGCCCGTCCAGATTCGTTTCT
CGACAAGATCTCCGTCTATTGCGCCCGGGTGCGGATGGGGCAGAAGCTGGGCGAGAAGATCGCACGTGAATGGGAGGATC
TGGATATCGACGTAGTGATCCCGATCCCGGAAACCTCCTGCGATATCGCGCTGCAGATCGCCCGTATTCTGGATAAGCCC
TACCGCCAGGGGTTTGTCAAAAACCGCTATGTGGGGCGTACCTTTATCATGCCGGGGCAGCAGCTGCGCCGCCAATCGGT
GCGCCGCAAGCTGAACGCCAATCGCGCCGAGTTTCGCGATAAGAATGTGCTGCTGGTGGATGACTCCATCGTGCGCGGCA
CCACCTCGGAGCAGATCATCGAGATGGCACGGGAGGCGGGCGCCCGTCGGGTTTATCTGGCCTCCGCCGCGCCGGAGATC
CGTTTTCCCAACGTCTATGGCATCGATATGCCGAGCGCGAGCGAGCTTATCGCCCACGGGCGCGAGGTCGAGGAGATCCG
CCAGCTGATCGGCGCCGATGGGCTGATTTTCCAGGATTTGACGGATCTTATCGAGGCTGTGCGAGAGGATAACCCGGATA
TCGAGCAGTTTGAGTGTTCGGTATTTGACGGGGTATATATCACGCGCGACGTGGATCAGGCCTATCTGGACTATCTGCAA
TCGCTGCGCGGCGATGATACCAAGGCGCTGAATAACCAGCAGCAGGCGGAAGATCTCGCGTTGTATAATGAAGGGTAG

Upstream 100 bases:

>100_bases
CATCAGGTGGTCATTTGACTATCTGCAAAGCACGTCGAGTTTCTTGCCGGAGCACATGCCGTCGCTGCCGCTCCGGTAAC
GCTGATGAGGAAATGACAAC

Downstream 100 bases:

>100_bases
CAGCACGTTTTGCGCTTTGCCGCCGGGCCGCATATGCGGCCCGTTTTTACTTGCACGCTCGCCCGCCTTGCGGCAAAGTC
GCTGCAGCAAAGCGTTGAAT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MCGIVGIAGFTPVNQSIYDALTVLQHRGQDAAGIVTIDDNHAFRLRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGS
SSASEAQPFYVNSPFGITLAHNGNLTNAHQLKQHLFEHARRHVNTTSDSEILLNIFAYELDRYDHYPLSDENIFAAVAAT
HRQIRGAYACVAMIIGHGMVAFRDPNGIRPLVIGKRTLEDGRTEYMVASESVALDTLGFDFIRDVAPGEAIYVNLQGQLF
TRQCAQDPKSHPCLFEYVYFARPDSFLDKISVYCARVRMGQKLGEKIAREWEDLDIDVVIPIPETSCDIALQIARILDKP
YRQGFVKNRYVGRTFIMPGQQLRRQSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGARRVYLASAAPEI
RFPNVYGIDMPSASELIAHGREVEEIRQLIGADGLIFQDLTDLIEAVREDNPDIEQFECSVFDGVYITRDVDQAYLDYLQ
SLRGDDTKALNNQQQAEDLALYNEG

Sequences:

>Translated_505_residues
MCGIVGIAGFTPVNQSIYDALTVLQHRGQDAAGIVTIDDNHAFRLRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGS
SSASEAQPFYVNSPFGITLAHNGNLTNAHQLKQHLFEHARRHVNTTSDSEILLNIFAYELDRYDHYPLSDENIFAAVAAT
HRQIRGAYACVAMIIGHGMVAFRDPNGIRPLVIGKRTLEDGRTEYMVASESVALDTLGFDFIRDVAPGEAIYVNLQGQLF
TRQCAQDPKSHPCLFEYVYFARPDSFLDKISVYCARVRMGQKLGEKIAREWEDLDIDVVIPIPETSCDIALQIARILDKP
YRQGFVKNRYVGRTFIMPGQQLRRQSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGARRVYLASAAPEI
RFPNVYGIDMPSASELIAHGREVEEIRQLIGADGLIFQDLTDLIEAVREDNPDIEQFECSVFDGVYITRDVDQAYLDYLQ
SLRGDDTKALNNQQQAEDLALYNEG
>Mature_505_residues
MCGIVGIAGFTPVNQSIYDALTVLQHRGQDAAGIVTIDDNHAFRLRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGS
SSASEAQPFYVNSPFGITLAHNGNLTNAHQLKQHLFEHARRHVNTTSDSEILLNIFAYELDRYDHYPLSDENIFAAVAAT
HRQIRGAYACVAMIIGHGMVAFRDPNGIRPLVIGKRTLEDGRTEYMVASESVALDTLGFDFIRDVAPGEAIYVNLQGQLF
TRQCAQDPKSHPCLFEYVYFARPDSFLDKISVYCARVRMGQKLGEKIAREWEDLDIDVVIPIPETSCDIALQIARILDKP
YRQGFVKNRYVGRTFIMPGQQLRRQSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGARRVYLASAAPEI
RFPNVYGIDMPSASELIAHGREVEEIRQLIGADGLIFQDLTDLIEAVREDNPDIEQFECSVFDGVYITRDVDQAYLDYLQ
SLRGDDTKALNNQQQAEDLALYNEG

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=473, Percent_Identity=35.7293868921776, Blast_Score=270, Evalue=3e-72,
Organism=Escherichia coli, GI1788651, Length=505, Percent_Identity=85.1485148514851, Blast_Score=908, Evalue=0.0,
Organism=Escherichia coli, GI1790167, Length=223, Percent_Identity=31.390134529148, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17554892, Length=463, Percent_Identity=33.0453563714903, Blast_Score=226, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6323958, Length=509, Percent_Identity=50.4911591355599, Blast_Score=502, Evalue=1e-143,
Organism=Drosophila melanogaster, GI24659598, Length=468, Percent_Identity=33.7606837606838, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24659604, Length=461, Percent_Identity=33.8394793926247, Blast_Score=242, Evalue=4e-64,
Organism=Drosophila melanogaster, GI28573187, Length=475, Percent_Identity=33.8947368421053, Blast_Score=238, Evalue=7e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 56722; Mature: 56722

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGIAGFTPVNQSIYDALTVLQHRGQDAAGIVTIDDNHAFRLRKANGLVSDVFEARH
CCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHH
MQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPFGITLAHNGNLTNAHQLKQHLFEHAR
HHHHHCCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHH
RHVNTTSDSEILLNIFAYELDRYDHYPLSDENIFAAVAATHRQIRGAYACVAMIIGHGMV
HHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEE
AFRDPNGIRPLVIGKRTLEDGRTEYMVASESVALDTLGFDFIRDVAPGEAIYVNLQGQLF
EEECCCCCCEEEEECHHHCCCCCEEEEECCCHHHHHHCHHHHHHCCCCCEEEEEECCHHH
TRQCAQDPKSHPCLFEYVYFARPDSFLDKISVYCARVRMGQKLGEKIAREWEDLDIDVVI
HHHHHCCCCCCCHHEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
PIPETSCDIALQIARILDKPYRQGFVKNRYVGRTFIMPGQQLRRQSVRRKLNANRAEFRD
ECCCCCHHHHHHHHHHHCCHHHCCHHHCCCCCEEEECCCHHHHHHHHHHHHCCCHHHHCC
KNVLLVDDSIVRGTTSEQIIEMAREAGARRVYLASAAPEIRFPNVYGIDMPSASELIAHG
CCEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCHHHHHHCC
REVEEIRQLIGADGLIFQDLTDLIEAVREDNPDIEQFECSVFDGVYITRDVDQAYLDYLQ
CCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHEEHEEECCEEEEECHHHHHHHHHH
SLRGDDTKALNNQQQAEDLALYNEG
HHCCCCHHHHCCHHHHHHHHCCCCC
>Mature Secondary Structure
MCGIVGIAGFTPVNQSIYDALTVLQHRGQDAAGIVTIDDNHAFRLRKANGLVSDVFEARH
CCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHH
MQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPFGITLAHNGNLTNAHQLKQHLFEHAR
HHHHHCCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHH
RHVNTTSDSEILLNIFAYELDRYDHYPLSDENIFAAVAATHRQIRGAYACVAMIIGHGMV
HHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEE
AFRDPNGIRPLVIGKRTLEDGRTEYMVASESVALDTLGFDFIRDVAPGEAIYVNLQGQLF
EEECCCCCCEEEEECHHHCCCCCEEEEECCCHHHHHHCHHHHHHCCCCCEEEEEECCHHH
TRQCAQDPKSHPCLFEYVYFARPDSFLDKISVYCARVRMGQKLGEKIAREWEDLDIDVVI
HHHHHCCCCCCCHHEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
PIPETSCDIALQIARILDKPYRQGFVKNRYVGRTFIMPGQQLRRQSVRRKLNANRAEFRD
ECCCCCHHHHHHHHHHHCCHHHCCHHHCCCCCEEEECCCHHHHHHHHHHHHCCCHHHHCC
KNVLLVDDSIVRGTTSEQIIEMAREAGARRVYLASAAPEIRFPNVYGIDMPSASELIAHG
CCEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCHHHHHHCC
REVEEIRQLIGADGLIFQDLTDLIEAVREDNPDIEQFECSVFDGVYITRDVDQAYLDYLQ
CCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHEEHEEECCEEEEECHHHHHHHHHH
SLRGDDTKALNNQQQAEDLALYNEG
HHCCCCHHHHCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3047685; 6443594; 6277938; 9205837; 9278503; 3040734; 7037784; 9333323; 9514258 [H]