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The map label for this gene is menD

Identifier: 238920538

GI number: 238920538

Start: 2544011

End: 2545690

Strand: Reverse

Name: menD

Synonym: NT01EI_2650

Alternate gene names: 238920538

Gene position: 2545690-2544011 (Counterclockwise)

Preceding gene: 238920539

Following gene: 238920537

Centisome position: 66.78

GC content: 66.43

Gene sequence:

>1680_bases
ATGTCATCTATTCTGTTTAACCGTCGCTGGGCCTCGGTGCTGCTTGAAGCGTTGACCCGTCACGGCGTACATCATGTCTG
TATCGCTCCCGGTTCCCGCTCTACGCCGCTGACGATGGCGGCAGCATCCCATCCCCGCCTGCATAGTCATACCCATTTCG
ACGAGCGCGGCCTAGGGCATTTGGCGCTGGGGCTGGCCAAAGTCAGTGGTCAGCCGGTGGCGGTGATCGTCACGTCGGGA
ACGGCCGTTGCCAATCTCTATCCGGCGCTGATCGAGGCCGGTCTGACCGGAGAGCGGCTGATCCTGCTGACCGCCGACCG
TCCGCCAGAGCTGATCGACTGCGGTGCCAACCAGGCGATCCGCCAACAGAACATGTTCGCCTCCCACCCGACGGAAACCC
TCAGTCTTCCCCGGCCTACGCCAGATATCTCCGCTGCCTGGCTGGTTTCCAGCATCGATAGCGCCATGGCCCGCCTCGAC
CACGGCGCACTGCACATCAACTGCCCATTCGCAGATCCGCTGTACGGCGAGGATGACGATGGACAGCAGGGGTGGTCTGC
GGCGCTGGGCTGCTGGTGGCAGGGCGAGACGCCCTGGCTGCAGGAGAGCTCCCCCTATACGGGCGCGCTGCGTGCAGAGG
TGGATTGGGCGCAGTGGCGCCGCCGTCGCGGGGTGGTGCTGATTGGCCGCGTTGACGCCGAAGCGGGCCGACAGGCGGCC
GCGTGGGGTGAGGAGCTGGGCTGGCCGGTGCTGGGGGATGTGTTGTCGCAAAGTGGCCAGCCGTTGCCCTGCGCCGATCT
GTGGCTTCCCCATGCAGAGGCGCGCCGCCTGCTGGCGCAGGCCGAGATTGTCGTGCAGTTTGGCGCCAGTCTGACCGGTA
AGCGTCTGCTGCAGTGGCAGGCAGCCTGCAAGCCACAGCGCTACTGGCTGATTGATCCGTTGCCGGGGCGGCTTGATCCC
GCCTGTCACCGCGGCCGCCGTCTGGTGGCGCCGGTGCAGGCATGGCTACAGGCACATCCTGCGCAGCCTGCCGCCCCCTG
GGCACCGCAGTTGAAGGTGCTGGCCGGGCGGGCTGCCCAGTGCGTGGAGCAAACGCTGCAGGCGTCGTTTGGTGAGGCGC
AGGTGGCGCACCGGCTGGCACAGCTGCTGCCGCTGCGGGGACAGCTGTTTGTCGGTAATAGCCTGATCGTGCGCCTGGTC
GATGCGCTGGGGCAGCTGCCTGCAGGCTATCCGGTATACAGTAACCGTGGCGCCAGCGGTATCGATGGACTGGTTTCAAC
CATTGCCGGGGTACAGCGCGGCAGCGGGCTGCCGACGCTGGCGATCCTGGGCGATCTGTCGGCGTTGTACGATATTAACG
GTCTGGCGCTACTGCGTCAGGTGACTGCGCCGACGGTGGTGATCGTGGTGAATAACAACGGTGGGCAGATTTTCTCCATG
CTGCCAACGCCACCGGCGGAGCGCGAGCGCTTTTACCTGATGCCGCAGAATGTGGACTTCCGCCACGCGGCGGCGCTGTT
TGATTTGGACTACGCCTGCCCGGTGGACTGGGCGGCGCTGACCGACGCGGTCTCTCAGGGCTGGCGGCGTTGCGGGACGA
CGCTGATCGAACTGCGGGTACCCGGCACGGCGGGCGCGGAGGCGCTGCAGCAGCTGCTGGGTAAGGTGGCCGCGCTGTGA

Upstream 100 bases:

>100_bases
TGGGGTGTGCTGTACACCAACGCGCGCGTCAGGAAGCCGCTGCCAGCCTGCCCGCTGCAGCTGCAGGACGTGCAATCCGC
TAAGCCACTGTTGAGTCGCC

Downstream 100 bases:

>100_bases
TCCTGGCCTATCGCCGTCTGGCGGCTGCGTCTCCCGATGCGCCGACCCTGGTATGGCTGCACGGTTTTCTCGGCAGTCAC
CGTGAGTGGCTGGCGCAGGC

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 559; Mature: 558

Protein sequence:

>559_residues
MSSILFNRRWASVLLEALTRHGVHHVCIAPGSRSTPLTMAAASHPRLHSHTHFDERGLGHLALGLAKVSGQPVAVIVTSG
TAVANLYPALIEAGLTGERLILLTADRPPELIDCGANQAIRQQNMFASHPTETLSLPRPTPDISAAWLVSSIDSAMARLD
HGALHINCPFADPLYGEDDDGQQGWSAALGCWWQGETPWLQESSPYTGALRAEVDWAQWRRRRGVVLIGRVDAEAGRQAA
AWGEELGWPVLGDVLSQSGQPLPCADLWLPHAEARRLLAQAEIVVQFGASLTGKRLLQWQAACKPQRYWLIDPLPGRLDP
ACHRGRRLVAPVQAWLQAHPAQPAAPWAPQLKVLAGRAAQCVEQTLQASFGEAQVAHRLAQLLPLRGQLFVGNSLIVRLV
DALGQLPAGYPVYSNRGASGIDGLVSTIAGVQRGSGLPTLAILGDLSALYDINGLALLRQVTAPTVVIVVNNNGGQIFSM
LPTPPAERERFYLMPQNVDFRHAAALFDLDYACPVDWAALTDAVSQGWRRCGTTLIELRVPGTAGAEALQQLLGKVAAL

Sequences:

>Translated_559_residues
MSSILFNRRWASVLLEALTRHGVHHVCIAPGSRSTPLTMAAASHPRLHSHTHFDERGLGHLALGLAKVSGQPVAVIVTSG
TAVANLYPALIEAGLTGERLILLTADRPPELIDCGANQAIRQQNMFASHPTETLSLPRPTPDISAAWLVSSIDSAMARLD
HGALHINCPFADPLYGEDDDGQQGWSAALGCWWQGETPWLQESSPYTGALRAEVDWAQWRRRRGVVLIGRVDAEAGRQAA
AWGEELGWPVLGDVLSQSGQPLPCADLWLPHAEARRLLAQAEIVVQFGASLTGKRLLQWQAACKPQRYWLIDPLPGRLDP
ACHRGRRLVAPVQAWLQAHPAQPAAPWAPQLKVLAGRAAQCVEQTLQASFGEAQVAHRLAQLLPLRGQLFVGNSLIVRLV
DALGQLPAGYPVYSNRGASGIDGLVSTIAGVQRGSGLPTLAILGDLSALYDINGLALLRQVTAPTVVIVVNNNGGQIFSM
LPTPPAERERFYLMPQNVDFRHAAALFDLDYACPVDWAALTDAVSQGWRRCGTTLIELRVPGTAGAEALQQLLGKVAAL
>Mature_558_residues
SSILFNRRWASVLLEALTRHGVHHVCIAPGSRSTPLTMAAASHPRLHSHTHFDERGLGHLALGLAKVSGQPVAVIVTSGT
AVANLYPALIEAGLTGERLILLTADRPPELIDCGANQAIRQQNMFASHPTETLSLPRPTPDISAAWLVSSIDSAMARLDH
GALHINCPFADPLYGEDDDGQQGWSAALGCWWQGETPWLQESSPYTGALRAEVDWAQWRRRRGVVLIGRVDAEAGRQAAA
WGEELGWPVLGDVLSQSGQPLPCADLWLPHAEARRLLAQAEIVVQFGASLTGKRLLQWQAACKPQRYWLIDPLPGRLDPA
CHRGRRLVAPVQAWLQAHPAQPAAPWAPQLKVLAGRAAQCVEQTLQASFGEAQVAHRLAQLLPLRGQLFVGNSLIVRLVD
ALGQLPAGYPVYSNRGASGIDGLVSTIAGVQRGSGLPTLAILGDLSALYDINGLALLRQVTAPTVVIVVNNNGGQIFSML
PTPPAERERFYLMPQNVDFRHAAALFDLDYACPVDWAALTDAVSQGWRRCGTTLIELRVPGTAGAEALQQLLGKVAAL

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=559, Percent_Identity=67.6207513416816, Blast_Score=758, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_EDWI9 (C5B7I2)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934053.1
- ProteinModelPortal:   C5B7I2
- GeneID:   7961676
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_2650
- OMA:   FYHDLNG
- ProtClustDB:   PRK07449
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 60185; Mature: 60053

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSILFNRRWASVLLEALTRHGVHHVCIAPGSRSTPLTMAAASHPRLHSHTHFDERGLGH
CCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCH
LALGLAKVSGQPVAVIVTSGTAVANLYPALIEAGLTGERLILLTADRPPELIDCGANQAI
HHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHEECCCCHHH
RQQNMFASHPTETLSLPRPTPDISAAWLVSSIDSAMARLDHGALHINCPFADPLYGEDDD
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCC
GQQGWSAALGCWWQGETPWLQESSPYTGALRAEVDWAQWRRRRGVVLIGRVDAEAGRQAA
CCHHHHHHHEEEECCCCCCCCCCCCCCCEEEECCCHHHHHHHCCEEEEEECCHHCCCHHH
AWGEELGWPVLGDVLSQSGQPLPCADLWLPHAEARRLLAQAEIVVQFGASLTGKRLLQWQ
HHHHHCCCHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AACKPQRYWLIDPLPGRLDPACHRGRRLVAPVQAWLQAHPAQPAAPWAPQLKVLAGRAAQ
HCCCCCCEEEECCCCCCCCHHHHCCCEEHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
CVEQTLQASFGEAQVAHRLAQLLPLRGQLFVGNSLIVRLVDALGQLPAGYPVYSNRGASG
HHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCH
IDGLVSTIAGVQRGSGLPTLAILGDLSALYDINGLALLRQVTAPTVVIVVNNNGGQIFSM
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEEECCCCEEEEE
LPTPPAERERFYLMPQNVDFRHAAALFDLDYACPVDWAALTDAVSQGWRRCGTTLIELRV
CCCCCCCCCEEEECCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEC
PGTAGAEALQQLLGKVAAL
CCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSILFNRRWASVLLEALTRHGVHHVCIAPGSRSTPLTMAAASHPRLHSHTHFDERGLGH
CCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCH
LALGLAKVSGQPVAVIVTSGTAVANLYPALIEAGLTGERLILLTADRPPELIDCGANQAI
HHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHEECCCCHHH
RQQNMFASHPTETLSLPRPTPDISAAWLVSSIDSAMARLDHGALHINCPFADPLYGEDDD
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCC
GQQGWSAALGCWWQGETPWLQESSPYTGALRAEVDWAQWRRRRGVVLIGRVDAEAGRQAA
CCHHHHHHHEEEECCCCCCCCCCCCCCCEEEECCCHHHHHHHCCEEEEEECCHHCCCHHH
AWGEELGWPVLGDVLSQSGQPLPCADLWLPHAEARRLLAQAEIVVQFGASLTGKRLLQWQ
HHHHHCCCHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AACKPQRYWLIDPLPGRLDPACHRGRRLVAPVQAWLQAHPAQPAAPWAPQLKVLAGRAAQ
HCCCCCCEEEECCCCCCCCHHHHCCCEEHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
CVEQTLQASFGEAQVAHRLAQLLPLRGQLFVGNSLIVRLVDALGQLPAGYPVYSNRGASG
HHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCH
IDGLVSTIAGVQRGSGLPTLAILGDLSALYDINGLALLRQVTAPTVVIVVNNNGGQIFSM
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEEECCCCEEEEE
LPTPPAERERFYLMPQNVDFRHAAALFDLDYACPVDWAALTDAVSQGWRRCGTTLIELRV
CCCCCCCCCEEEECCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEC
PGTAGAEALQQLLGKVAAL
CCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA