Click here to switch to the map view.

The map label for this gene is menF [H]

Identifier: 238920539

GI number: 238920539

Start: 2545929

End: 2547257

Strand: Reverse

Name: menF [H]

Synonym: NT01EI_2651

Alternate gene names: 238920539

Gene position: 2547257-2545929 (Counterclockwise)

Preceding gene: 238920540

Following gene: 238920538

Centisome position: 66.82

GC content: 63.51

Gene sequence:

>1329_bases
GTGCACCATTTTACTGCTCTGATACAACGCATTGTCCCCCAGCTACAGAAAGCGGCCCAGCAGGGTCCGGGACTGGTCAG
AGTGGCGCTGCCCTTTGCGGCGGATGACAGTGATTCGATGCTGGCGTGGCTGGCGACCCAGCCGCTGTATCCGCAGTTTT
ACTGGCGCCACCGCAGTGATGAGGAGGAGGCCGCGGTGTGTGGGGCGCTGAAGGTGTTTCGCGATGCCGGGCAGGCGCAG
GCGTTTATCCGGGGGTACAACCATCCGGATCTGCGGATCTGGGGGCTGAATGCTTTCGACCGCGTGGCATTGGATGGCTA
TGGTGAGGTGGAGAGCCTGCTGTTTTTACCGCGTCTGGCGCTGCTGCGCTACGGTGATAGGGCTGAGCTGGTGGTATACC
TGTACAGCGAGACATCGCTGCAGGAGGATCTGCCGCGGGCGTTGAGCGATCTGGCCACGCTGCTGCCGCCGCAGCCGCTG
GGGTCCCTACAGGCTCAGGTGCTGAGCGCCGCCCATCAGCCCGATCACGGCGCCTGGTGCGCGCTGCTGACCCAGGCGCT
GGCAGCGATCGCCCGCAATGAATTTGCCAAGGTAGTGATGGCGCGCCGTACCACCTTAACCCTCTGTGAGCCACTGCGCG
CAGCACAGTTTCTGGCCGCCAGCCGGGCAGTCAACCACCGTTGCTACCACTTTATGCTGGCGTTGGCGCCGCACCGGGCA
TTTTTGGGATCGTCGCCGGAGCGGCTGTACCGCCGTATTGGTCGGCAGCTGGAGACCGAGGCGCTGGCCGGTACCGTCGC
CCGCGGTGAAAACGAGGTGCAGCGCCGGGCTAACGCGGCATGGCTGATGCAAGATGGCAAGAATCAGCATGAAAATCTGC
TGGTGGTTGATGATATCTGCCAGCGCCTGTGCAGCGGCGCGGGTGCGCCGACGCTGGAGGTGATGGCGGCGGAGATTGTG
GCGCTGCGCAAGGTACAGCATCTGCGTCGCAGCATCCATGTGATTCTCAACCGGCAGGATGATGCTGAATGCCTGCGCCG
CCTGCAGCCTACAGCGGCGGTGGCTGGACTGCCTCGTGAGCCCGCACGCTATTTTATTATGCGCGATGAGCCCTTTGCGC
GCGGCTGGTATGCGGGCTCTGCAGGCTACCTTGCGCTGGCGCAGGCCGAGTTTGCCGTTGCGTTGCGTTCGGCGCTGATC
GAAGACGAGCGTATCCATTTGTATGCCGGTGCGGGTATCGTGGCGGGCTCGGATCCGCAGCAAGAGTGGCTGGAAATTGA
GAATAAGGCGGCCGGTCTGCGTACGCTGCTGGAGGGCGAGAGCCTGTAG

Upstream 100 bases:

>100_bases
GCCAGTGGGAGCGATATCACCCTCATCCGCTTTTTTTCTCTTTAATCCCCCGTATAATTCACCACTCTCCTAACCGCTGT
TATCCCCGACCGAGGTTGTC

Downstream 100 bases:

>100_bases
CATCGGAGACAAAAGCTGCGATCGGGGCGGTATATGCGCAGTACACGGATTGTGATATGGATGAGGCTGGTGTACCATAT
TGGTCATATTTTATATTTGA

Product: isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MHHFTALIQRIVPQLQKAAQQGPGLVRVALPFAADDSDSMLAWLATQPLYPQFYWRHRSDEEEAAVCGALKVFRDAGQAQ
AFIRGYNHPDLRIWGLNAFDRVALDGYGEVESLLFLPRLALLRYGDRAELVVYLYSETSLQEDLPRALSDLATLLPPQPL
GSLQAQVLSAAHQPDHGAWCALLTQALAAIARNEFAKVVMARRTTLTLCEPLRAAQFLAASRAVNHRCYHFMLALAPHRA
FLGSSPERLYRRIGRQLETEALAGTVARGENEVQRRANAAWLMQDGKNQHENLLVVDDICQRLCSGAGAPTLEVMAAEIV
ALRKVQHLRRSIHVILNRQDDAECLRRLQPTAAVAGLPREPARYFIMRDEPFARGWYAGSAGYLALAQAEFAVALRSALI
EDERIHLYAGAGIVAGSDPQQEWLEIENKAAGLRTLLEGESL

Sequences:

>Translated_442_residues
MHHFTALIQRIVPQLQKAAQQGPGLVRVALPFAADDSDSMLAWLATQPLYPQFYWRHRSDEEEAAVCGALKVFRDAGQAQ
AFIRGYNHPDLRIWGLNAFDRVALDGYGEVESLLFLPRLALLRYGDRAELVVYLYSETSLQEDLPRALSDLATLLPPQPL
GSLQAQVLSAAHQPDHGAWCALLTQALAAIARNEFAKVVMARRTTLTLCEPLRAAQFLAASRAVNHRCYHFMLALAPHRA
FLGSSPERLYRRIGRQLETEALAGTVARGENEVQRRANAAWLMQDGKNQHENLLVVDDICQRLCSGAGAPTLEVMAAEIV
ALRKVQHLRRSIHVILNRQDDAECLRRLQPTAAVAGLPREPARYFIMRDEPFARGWYAGSAGYLALAQAEFAVALRSALI
EDERIHLYAGAGIVAGSDPQQEWLEIENKAAGLRTLLEGESL
>Mature_442_residues
MHHFTALIQRIVPQLQKAAQQGPGLVRVALPFAADDSDSMLAWLATQPLYPQFYWRHRSDEEEAAVCGALKVFRDAGQAQ
AFIRGYNHPDLRIWGLNAFDRVALDGYGEVESLLFLPRLALLRYGDRAELVVYLYSETSLQEDLPRALSDLATLLPPQPL
GSLQAQVLSAAHQPDHGAWCALLTQALAAIARNEFAKVVMARRTTLTLCEPLRAAQFLAASRAVNHRCYHFMLALAPHRA
FLGSSPERLYRRIGRQLETEALAGTVARGENEVQRRANAAWLMQDGKNQHENLLVVDDICQRLCSGAGAPTLEVMAAEIV
ALRKVQHLRRSIHVILNRQDDAECLRRLQPTAAVAGLPREPARYFIMRDEPFARGWYAGSAGYLALAQAEFAVALRSALI
EDERIHLYAGAGIVAGSDPQQEWLEIENKAAGLRTLLEGESL

Specific function: Menaquinone biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI87082077, Length=441, Percent_Identity=51.0204081632653, Blast_Score=407, Evalue=1e-115,
Organism=Escherichia coli, GI1786809, Length=271, Percent_Identity=29.520295202952, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1787518, Length=295, Percent_Identity=29.1525423728814, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788114, Length=202, Percent_Identity=28.7128712871287, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320935, Length=229, Percent_Identity=28.82096069869, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 49049; Mature: 49049

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHHFTALIQRIVPQLQKAAQQGPGLVRVALPFAADDSDSMLAWLATQPLYPQFYWRHRSD
CCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEHHHHCCCCCHHHHHCCCCC
EEEAAVCGALKVFRDAGQAQAFIRGYNHPDLRIWGLNAFDRVALDGYGEVESLLFLPRLA
HHHHHHHHHHHHHHHCHHHHHHHHCCCCCCEEEECCCHHHHHHCCCCCHHHHHHHHHHHH
LLRYGDRAELVVYLYSETSLQEDLPRALSDLATLLPPQPLGSLQAQVLSAAHQPDHGAWC
HHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHH
ALLTQALAAIARNEFAKVVMARRTTLTLCEPLRAAQFLAASRAVNHRCYHFMLALAPHRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLGSSPERLYRRIGRQLETEALAGTVARGENEVQRRANAAWLMQDGKNQHENLLVVDDIC
HHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHH
QRLCSGAGAPTLEVMAAEIVALRKVQHLRRSIHVILNRQDDAECLRRLQPTAAVAGLPRE
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHCCCCCC
PARYFIMRDEPFARGWYAGSAGYLALAQAEFAVALRSALIEDERIHLYAGAGIVAGSDPQ
CCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCEEECCCCCH
QEWLEIENKAAGLRTLLEGESL
HHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MHHFTALIQRIVPQLQKAAQQGPGLVRVALPFAADDSDSMLAWLATQPLYPQFYWRHRSD
CCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEHHHHCCCCCHHHHHCCCCC
EEEAAVCGALKVFRDAGQAQAFIRGYNHPDLRIWGLNAFDRVALDGYGEVESLLFLPRLA
HHHHHHHHHHHHHHHCHHHHHHHHCCCCCCEEEECCCHHHHHHCCCCCHHHHHHHHHHHH
LLRYGDRAELVVYLYSETSLQEDLPRALSDLATLLPPQPLGSLQAQVLSAAHQPDHGAWC
HHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHH
ALLTQALAAIARNEFAKVVMARRTTLTLCEPLRAAQFLAASRAVNHRCYHFMLALAPHRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLGSSPERLYRRIGRQLETEALAGTVARGENEVQRRANAAWLMQDGKNQHENLLVVDDIC
HHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHH
QRLCSGAGAPTLEVMAAEIVALRKVQHLRRSIHVILNRQDDAECLRRLQPTAAVAGLPRE
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHCCCCCC
PARYFIMRDEPFARGWYAGSAGYLALAQAEFAVALRSALIEDERIHLYAGAGIVAGSDPQ
CCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCEEECCCCCH
QEWLEIENKAAGLRTLLEGESL
HHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8549818; 8764478; 9205837; 9278503; 2666397; 7984428 [H]