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The map label for this gene is hisH [H]

Identifier: 238920457

GI number: 238920457

Start: 2460579

End: 2461184

Strand: Direct

Name: hisH [H]

Synonym: NT01EI_2568

Alternate gene names: 238920457

Gene position: 2460579-2461184 (Clockwise)

Preceding gene: 238920456

Following gene: 238920458

Centisome position: 64.54

GC content: 66.34

Gene sequence:

>606_bases
ATGGCCACAGCGTGCAACGTGGTGATTCTGGATACCGGCTGCGCCAACCTGGCCTCGGTGCGCTATGCCCTGGAGCGCCT
GGGCTGCCGGCCGACCGTCAGCCGCGATGCACAGGCGGTGCTGGCGGCTGACAAACTGCTGCTGCCCGGCGTCGGCAACG
CCGCCGCCGCCATGGAGCAGCTGCGCCAGCGCGATCTTATCGACCTGATCAGGGCCTGCACCCAGCCGGTGCTCGGCATC
TGCCTGGGGATGCAGCTGCTGGCCAGCCGCAGCGCCGAAGGGGATGTCCCCGGCCTCCATCTGATCGACACCCCGGTCGA
ACGGATGACCGACACCGGCCTGCCGCTGCCGCACACCGGCTGGAATCAGGTCACCTCTCTGGCCGGTAATCCCCTGTTTC
GCGGGATTCCCGACGGCAGCTACTTCTATTTCGTGCACAGCTACGCCTATCCCCTGTGCGCCGACACCCTGGCGCAGTGC
GACTACGGCTATACCTTTAGCGCCGCGGTGCGCCGCGATAACTTCTACGGCGTGCAGTTTCACCCCGAGCGCTCCGGCAA
GGCCGGTGCCCTCCTGCTGAAAAACTTTCTGGAGATGCCGTCATGA

Upstream 100 bases:

>100_bases
ACCACCGCGTAGAGAGCCTGTTCAAGGCCTTTGGCCGCACCCTGCGCCAGGCGATCCGCATCGAAGACGACCGCTTGCCC
AGCTCAAAAGGAGTATTGTG

Downstream 100 bases:

>100_bases
TCATTCCGGCACTGGACTTTATCGACGGCAGCGTCGTTCGCCTGCACCAGGGGGACTATGGCCGTCAGCGCGACTATGAT
GGCGATGCACTGGCGCGGCT

Product: imidazole glycerol phosphate synthase, glutamine amidotransferase subunit

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MATACNVVILDTGCANLASVRYALERLGCRPTVSRDAQAVLAADKLLLPGVGNAAAAMEQLRQRDLIDLIRACTQPVLGI
CLGMQLLASRSAEGDVPGLHLIDTPVERMTDTGLPLPHTGWNQVTSLAGNPLFRGIPDGSYFYFVHSYAYPLCADTLAQC
DYGYTFSAAVRRDNFYGVQFHPERSGKAGALLLKNFLEMPS

Sequences:

>Translated_201_residues
MATACNVVILDTGCANLASVRYALERLGCRPTVSRDAQAVLAADKLLLPGVGNAAAAMEQLRQRDLIDLIRACTQPVLGI
CLGMQLLASRSAEGDVPGLHLIDTPVERMTDTGLPLPHTGWNQVTSLAGNPLFRGIPDGSYFYFVHSYAYPLCADTLAQC
DYGYTFSAAVRRDNFYGVQFHPERSGKAGALLLKNFLEMPS
>Mature_200_residues
ATACNVVILDTGCANLASVRYALERLGCRPTVSRDAQAVLAADKLLLPGVGNAAAAMEQLRQRDLIDLIRACTQPVLGIC
LGMQLLASRSAEGDVPGLHLIDTPVERMTDTGLPLPHTGWNQVTSLAGNPLFRGIPDGSYFYFVHSYAYPLCADTLAQCD
YGYTFSAAVRRDNFYGVQFHPERSGKAGALLLKNFLEMPS

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=195, Percent_Identity=69.2307692307692, Blast_Score=271, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6319725, Length=212, Percent_Identity=33.9622641509434, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 21708; Mature: 21577

Theoretical pI: Translated: 6.49; Mature: 6.49

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATACNVVILDTGCANLASVRYALERLGCRPTVSRDAQAVLAADKLLLPGVGNAAAAMEQ
CCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHH
LRQRDLIDLIRACTQPVLGICLGMQLLASRSAEGDVPGLHLIDTPVERMTDTGLPLPHTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHCCCCCCCCC
WNQVTSLAGNPLFRGIPDGSYFYFVHSYAYPLCADTLAQCDYGYTFSAAVRRDNFYGVQF
HHHHHHHCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCEEHHEEECCCEEEEEE
HPERSGKAGALLLKNFLEMPS
CCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
ATACNVVILDTGCANLASVRYALERLGCRPTVSRDAQAVLAADKLLLPGVGNAAAAMEQ
CCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHH
LRQRDLIDLIRACTQPVLGICLGMQLLASRSAEGDVPGLHLIDTPVERMTDTGLPLPHTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHCCCCCCCCC
WNQVTSLAGNPLFRGIPDGSYFYFVHSYAYPLCADTLAQCDYGYTFSAAVRRDNFYGVQF
HHHHHHHCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCEEHHEEECCCEEEEEE
HPERSGKAGALLLKNFLEMPS
CCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]