Click here to switch to the map view.

The map label for this gene is hisA

Identifier: 238920458

GI number: 238920458

Start: 2461181

End: 2461918

Strand: Direct

Name: hisA

Synonym: NT01EI_2569

Alternate gene names: 238920458

Gene position: 2461181-2461918 (Clockwise)

Preceding gene: 238920457

Following gene: 238920459

Centisome position: 64.56

GC content: 66.94

Gene sequence:

>738_bases
ATGATCATTCCGGCACTGGACTTTATCGACGGCAGCGTCGTTCGCCTGCACCAGGGGGACTATGGCCGTCAGCGCGACTA
TGATGGCGATGCACTGGCGCGGCTGCTGGAGTATCAGCGTCAGGGCGCAGCACAGCTGCACCTGGTCGACCTGAGCGGCG
CGCGCGATCCCGCCCGCCGCCAGCTCCCCATGTTGACCCGCCTGCTGCGGGCATTGAGCGTGCCGGTACAGGTCGGCGGC
GGCGTTCGCAGCGCAGAGGACGTGGCCACACTGCTCGACGCCGGGGCGACGCGGGTGGTGGTCGGCTCAACGGCGGTCCG
CCAGCCGCAGGAGGTCGCCCGCTGGTTCACGCGCTTCGGCGCCGATCGGCTGGTGCTGGCGCTGGATGTACGTATCGCCG
CCAACGGCGATAAACGTGTGGCCGTCAGCGGCTGGCAAGAGGACTCCGGCGTGACCCTGGAACAGACGGTAGCGCATTTT
CTGCCGCTGGGACTACGGCACGTACTGTGTACCGACATCGCCTGCGACGGCACCCTGGGCGGCGCCAATGTGGCGCTGTA
CCGCCAGATCTGCCCGCGCTATCCGCAGATCGAATTTCAATCCTCCGGCGGCATCGGCGCGCTGCAGGACATTACTGCCC
TGCGCGGCAGCGGCGTGCAGGGCATCATCGTCGGCCGTGCCCTGCTGGAAGGCAAATTCAGCGTGAAGGAGGCCATCGCA
TGCTGGCAAAACGCATAA

Upstream 100 bases:

>100_bases
CCGCGGTGCGCCGCGATAACTTCTACGGCGTGCAGTTTCACCCCGAGCGCTCCGGCAAGGCCGGTGCCCTCCTGCTGAAA
AACTTTCTGGAGATGCCGTC

Downstream 100 bases:

>100_bases
TTCCCTGTCTGGACGTTCGCGACGGCCAGGTCGTCAAAGGCGTTCAGTTTCGCCATCACGAGATCATCGGCGATATCGTT
CCGCTGGCCCGGCGCTACGC

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MIIPALDFIDGSVVRLHQGDYGRQRDYDGDALARLLEYQRQGAAQLHLVDLSGARDPARRQLPMLTRLLRALSVPVQVGG
GVRSAEDVATLLDAGATRVVVGSTAVRQPQEVARWFTRFGADRLVLALDVRIAANGDKRVAVSGWQEDSGVTLEQTVAHF
LPLGLRHVLCTDIACDGTLGGANVALYRQICPRYPQIEFQSSGGIGALQDITALRGSGVQGIIVGRALLEGKFSVKEAIA
CWQNA

Sequences:

>Translated_245_residues
MIIPALDFIDGSVVRLHQGDYGRQRDYDGDALARLLEYQRQGAAQLHLVDLSGARDPARRQLPMLTRLLRALSVPVQVGG
GVRSAEDVATLLDAGATRVVVGSTAVRQPQEVARWFTRFGADRLVLALDVRIAANGDKRVAVSGWQEDSGVTLEQTVAHF
LPLGLRHVLCTDIACDGTLGGANVALYRQICPRYPQIEFQSSGGIGALQDITALRGSGVQGIIVGRALLEGKFSVKEAIA
CWQNA
>Mature_245_residues
MIIPALDFIDGSVVRLHQGDYGRQRDYDGDALARLLEYQRQGAAQLHLVDLSGARDPARRQLPMLTRLLRALSVPVQVGG
GVRSAEDVATLLDAGATRVVVGSTAVRQPQEVARWFTRFGADRLVLALDVRIAANGDKRVAVSGWQEDSGVTLEQTVAHF
LPLGLRHVLCTDIACDGTLGGANVALYRQICPRYPQIEFQSSGGIGALQDITALRGSGVQGIIVGRALLEGKFSVKEAIA
CWQNA

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=245, Percent_Identity=70.2040816326531, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1788336, Length=235, Percent_Identity=26.8085106382979, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_EDWI9 (C5BG15)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002933973.1
- ProteinModelPortal:   C5BG15
- GeneID:   7961757
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_2569
- OMA:   SIIYTDI
- ProtClustDB:   CLSK2813361
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 26353; Mature: 26353

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: NA

Important sites: ACT_SITE 7-7 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIPALDFIDGSVVRLHQGDYGRQRDYDGDALARLLEYQRQGAAQLHLVDLSGARDPARR
CCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHH
QLPMLTRLLRALSVPVQVGGGVRSAEDVATLLDAGATRVVVGSTAVRQPQEVARWFTRFG
HHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHCCCCEEEECCHHHCCHHHHHHHHHHHC
ADRLVLALDVRIAANGDKRVAVSGWQEDSGVTLEQTVAHFLPLGLRHVLCTDIACDGTLG
CCEEEEEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GANVALYRQICPRYPQIEFQSSGGIGALQDITALRGSGVQGIIVGRALLEGKFSVKEAIA
CCHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEHHHHHCCCHHHHHHHH
CWQNA
HHCCC
>Mature Secondary Structure
MIIPALDFIDGSVVRLHQGDYGRQRDYDGDALARLLEYQRQGAAQLHLVDLSGARDPARR
CCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHH
QLPMLTRLLRALSVPVQVGGGVRSAEDVATLLDAGATRVVVGSTAVRQPQEVARWFTRFG
HHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHCCCCEEEECCHHHCCHHHHHHHHHHHC
ADRLVLALDVRIAANGDKRVAVSGWQEDSGVTLEQTVAHFLPLGLRHVLCTDIACDGTLG
CCEEEEEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GANVALYRQICPRYPQIEFQSSGGIGALQDITALRGSGVQGIIVGRALLEGKFSVKEAIA
CCHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEHHHHHCCCHHHHHHHH
CWQNA
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA