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The map label for this gene is phoQ [H]

Identifier: 238920216

GI number: 238920216

Start: 2222975

End: 2224441

Strand: Direct

Name: phoQ [H]

Synonym: NT01EI_2325

Alternate gene names: 238920216

Gene position: 2222975-2224441 (Clockwise)

Preceding gene: 238920215

Following gene: 238920217

Centisome position: 58.31

GC content: 60.74

Gene sequence:

>1467_bases
ATGATGCTACAGCGCTGGCAGGCATGGAAAGCCCGCCCCTTATCGCTGCGCACCCGCTTTATGCTGGCTACCGCCGCGAT
GGTGTTCCTGCTCTCGCTGGCCTATGGCCTGGTGGCAGTGGTTGGCTACGCCGTCAGCTTCGACAAAACCACCTACCGTC
TGTTGCGCGGTGAGAGCAACCTGTTTTTTAGCCTGGCCCACTGGCAAGATGGACGCCTGACCATCACCCCTCCACCCAAC
ATGGCGCTGAATACGCCGACGCTGGTGATGATTTACGACCGTGAAGGACGCCTGCTCTGGTCTAAGCGTCAGATACCGGC
GCTGGAAAAACGCATTGAGCGCAGCTGGCTGGAGAAGCCGGGCTTTTATGAAATCGACAGCAACAAGTATTTCAGCAGCA
CGGTGCTCGGCGACAACCCGCGGATGCAGAATCAGCTGAAGGACTACGACGACGACAGCGCCGATGCCATGACCCACTCC
GTCGCCGTCAGCCAATATACTGCCACGGCGCACCTACCGGCGCTGACCATCGTGGTTGTCGATACCATCCCCCAGGAGCT
GCAAAACTCTGACATGGTCTGGGGCTGGTTCAGTTATGTACTGATAGCCAACGTGCTGTTGGTGATCCCCCTGCTGTGGC
TCGCCGCCTTCTGGAGCCTGCAACCGATCAAGGCCCTGGCCACCCAGGTACGCGAGCTGGAGAAGGGGCAGCGCGATAGC
CTCAGCGAAAATCCCCCTCATGAGCTGCGCGGTCTGGTGCGCAACCTCAACATCCTGCTGAACAACGAGCGCCAGCGCTA
TACCCGCTACCGCACCACCCTTGCCGATCTGACCCACAGCCTGAAGACCCCGCTGGCGGTGCTACAGAGCACCCTGCGTT
CCCTGCGCAACGGGAAAAGTCTGAGCGTCGAACAGGCCGAGCCGATCATGCTGGAGCAGATCAGCCGTATCTCCCAGCAG
ATCGGCTACTACCTGCACCGCGCCAGCGCCCATTCGGAACACAGCGTGCTCAACCGCGAGGTGCACTCGGTATCAGCGAT
CCTCGACGCCCTGACCTCGGCGCTGAACAAAGTCTACCAGCGCAAGGGGGTGGACATCTCGCTGGATATCCCGCCGGAGC
TCACCTTTATCGGTGAAAAGAACGATCTGATGGAGGTGATCGGAAACCTGCTGGATAACGCCTGCAAATACTGCCTGGAA
TTTGTGGAGGTCAGCGCCAGCCTGCACGGCGGCGATCTGATGCTATGGGTAGACGACGACGGCCCCGGCATTCCACACGA
CAAACGCGAGCTGGTGTTCGCCCGCGGTCAGCGCGCTGATACCCTGCGCCCCGGCCAGGGGCTGGGGCTGGCACTGGTGG
ACGAACTGGTCAATCAGTACGACGGCAGCATCGCTGTCGATGACAGTCCACTGGGCGGCGCACGCATCTGCGTACGCTTT
GGCCAGCAACAGCCGGATCGCAATTAA

Upstream 100 bases:

>100_bases
ACGTACTGATGGGGCGCCTGCGCAAAAAGCTGCAGAGTGAATACCCGGCCGATGTCATCACCACCGTACGCGGTCAGGGC
TACCGCTTCGACGTGCCGGC

Downstream 100 bases:

>100_bases
ATCGACCACCGGCCGCACAATATTCCCCTTAGCCCGGCGGATCGCCGCGCGTCATCCGTTATACTGGGCAGAGTCCCGCC
ACGCGATGTAACCCGATATC

Product: sensor protein PhoQ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MMLQRWQAWKARPLSLRTRFMLATAAMVFLLSLAYGLVAVVGYAVSFDKTTYRLLRGESNLFFSLAHWQDGRLTITPPPN
MALNTPTLVMIYDREGRLLWSKRQIPALEKRIERSWLEKPGFYEIDSNKYFSSTVLGDNPRMQNQLKDYDDDSADAMTHS
VAVSQYTATAHLPALTIVVVDTIPQELQNSDMVWGWFSYVLIANVLLVIPLLWLAAFWSLQPIKALATQVRELEKGQRDS
LSENPPHELRGLVRNLNILLNNERQRYTRYRTTLADLTHSLKTPLAVLQSTLRSLRNGKSLSVEQAEPIMLEQISRISQQ
IGYYLHRASAHSEHSVLNREVHSVSAILDALTSALNKVYQRKGVDISLDIPPELTFIGEKNDLMEVIGNLLDNACKYCLE
FVEVSASLHGGDLMLWVDDDGPGIPHDKRELVFARGQRADTLRPGQGLGLALVDELVNQYDGSIAVDDSPLGGARICVRF
GQQQPDRN

Sequences:

>Translated_488_residues
MMLQRWQAWKARPLSLRTRFMLATAAMVFLLSLAYGLVAVVGYAVSFDKTTYRLLRGESNLFFSLAHWQDGRLTITPPPN
MALNTPTLVMIYDREGRLLWSKRQIPALEKRIERSWLEKPGFYEIDSNKYFSSTVLGDNPRMQNQLKDYDDDSADAMTHS
VAVSQYTATAHLPALTIVVVDTIPQELQNSDMVWGWFSYVLIANVLLVIPLLWLAAFWSLQPIKALATQVRELEKGQRDS
LSENPPHELRGLVRNLNILLNNERQRYTRYRTTLADLTHSLKTPLAVLQSTLRSLRNGKSLSVEQAEPIMLEQISRISQQ
IGYYLHRASAHSEHSVLNREVHSVSAILDALTSALNKVYQRKGVDISLDIPPELTFIGEKNDLMEVIGNLLDNACKYCLE
FVEVSASLHGGDLMLWVDDDGPGIPHDKRELVFARGQRADTLRPGQGLGLALVDELVNQYDGSIAVDDSPLGGARICVRF
GQQQPDRN
>Mature_488_residues
MMLQRWQAWKARPLSLRTRFMLATAAMVFLLSLAYGLVAVVGYAVSFDKTTYRLLRGESNLFFSLAHWQDGRLTITPPPN
MALNTPTLVMIYDREGRLLWSKRQIPALEKRIERSWLEKPGFYEIDSNKYFSSTVLGDNPRMQNQLKDYDDDSADAMTHS
VAVSQYTATAHLPALTIVVVDTIPQELQNSDMVWGWFSYVLIANVLLVIPLLWLAAFWSLQPIKALATQVRELEKGQRDS
LSENPPHELRGLVRNLNILLNNERQRYTRYRTTLADLTHSLKTPLAVLQSTLRSLRNGKSLSVEQAEPIMLEQISRISQQ
IGYYLHRASAHSEHSVLNREVHSVSAILDALTSALNKVYQRKGVDISLDIPPELTFIGEKNDLMEVIGNLLDNACKYCLE
FVEVSASLHGGDLMLWVDDDGPGIPHDKRELVFARGQRADTLRPGQGLGLALVDELVNQYDGSIAVDDSPLGGARICVRF
GQQQPDRN

Specific function: Member of the two-component regulatory system phoQ/phoP which regulates the expression of genes involved in virulence and resistance to host defense antimicrobial peptides. In presence of low periplasmic Mg(2+) concentrations, phoQ functions as a membrane

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787374, Length=473, Percent_Identity=62.1564482029598, Blast_Score=593, Evalue=1e-170,
Organism=Escherichia coli, GI1790346, Length=289, Percent_Identity=26.9896193771626, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788393, Length=273, Percent_Identity=26.007326007326, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1786912, Length=220, Percent_Identity=32.7272727272727, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787894, Length=325, Percent_Identity=26.7692307692308, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87082128, Length=336, Percent_Identity=25.8928571428571, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786783, Length=285, Percent_Identity=23.5087719298246, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789808, Length=289, Percent_Identity=26.2975778546713, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1786600, Length=218, Percent_Identity=27.0642201834862, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790861, Length=263, Percent_Identity=27.3764258555133, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI145693157, Length=227, Percent_Identity=28.6343612334802, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR015014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF08918 PhoQ_Sensor [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 55056; Mature: 55056

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLQRWQAWKARPLSLRTRFMLATAAMVFLLSLAYGLVAVVGYAVSFDKTTYRLLRGESN
CCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCC
LFFSLAHWQDGRLTITPPPNMALNTPTLVMIYDREGRLLWSKRQIPALEKRIERSWLEKP
EEEEEEECCCCEEEECCCCCCEECCCEEEEEECCCCCEEECCCCCCHHHHHHHHHHCCCC
GFYEIDSNKYFSSTVLGDNPRMQNQLKDYDDDSADAMTHSVAVSQYTATAHLPALTIVVV
CCEEECCCCEEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEE
DTIPQELQNSDMVWGWFSYVLIANVLLVIPLLWLAAFWSLQPIKALATQVRELEKGQRDS
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
LSENPPHELRGLVRNLNILLNNERQRYTRYRTTLADLTHSLKTPLAVLQSTLRSLRNGKS
CCCCCCHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LSVEQAEPIMLEQISRISQQIGYYLHRASAHSEHSVLNREVHSVSAILDALTSALNKVYQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKGVDISLDIPPELTFIGEKNDLMEVIGNLLDNACKYCLEFVEVSASLHGGDLMLWVDDD
HCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
GPGIPHDKRELVFARGQRADTLRPGQGLGLALVDELVNQYDGSIAVDDSPLGGARICVRF
CCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHH
GQQQPDRN
CCCCCCCC
>Mature Secondary Structure
MMLQRWQAWKARPLSLRTRFMLATAAMVFLLSLAYGLVAVVGYAVSFDKTTYRLLRGESN
CCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCC
LFFSLAHWQDGRLTITPPPNMALNTPTLVMIYDREGRLLWSKRQIPALEKRIERSWLEKP
EEEEEEECCCCEEEECCCCCCEECCCEEEEEECCCCCEEECCCCCCHHHHHHHHHHCCCC
GFYEIDSNKYFSSTVLGDNPRMQNQLKDYDDDSADAMTHSVAVSQYTATAHLPALTIVVV
CCEEECCCCEEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEE
DTIPQELQNSDMVWGWFSYVLIANVLLVIPLLWLAAFWSLQPIKALATQVRELEKGQRDS
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
LSENPPHELRGLVRNLNILLNNERQRYTRYRTTLADLTHSLKTPLAVLQSTLRSLRNGKS
CCCCCCHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LSVEQAEPIMLEQISRISQQIGYYLHRASAHSEHSVLNREVHSVSAILDALTSALNKVYQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKGVDISLDIPPELTFIGEKNDLMEVIGNLLDNACKYCLEFVEVSASLHGGDLMLWVDDD
HCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
GPGIPHDKRELVFARGQRADTLRPGQGLGLALVDELVNQYDGSIAVDDSPLGGARICVRF
CCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHH
GQQQPDRN
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA