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The map label for this gene is mii [H]

Identifier: 238920067

GI number: 238920067

Start: 2099639

End: 2100814

Strand: Direct

Name: mii [H]

Synonym: NT01EI_2172

Alternate gene names: 238920067

Gene position: 2099639-2100814 (Clockwise)

Preceding gene: 238920066

Following gene: 238920068

Centisome position: 55.08

GC content: 65.65

Gene sequence:

>1176_bases
ATGCAAACTGAAGTGACTGCGCTCCCCGTCGCTATCGTACGCGGGGGCACCAGCAAGGGCATCTTCATCATGAAGAACGC
CCTCCCCGCCGATCCCGCCCTGCGCGATCGGGTGATCCTCTCCGTCTTCGGCAGCCCGGATCTGCGCCAGATCGATGGGC
TGGGCGGCGCCGATCCGCTGACCAGCAAGCTGGCCATCATCGCGCCCGCCAGCCGCCCCGACGCGGATGTGGATTATATC
TTCGGCCAGGTCAGCCTCAGCGAGGCCTTCATCGACTACGGCGGCAACTGCGGCAACATCTCGGCGGCGGTCGGCGCCTT
CGCGATCCAGAAGGGGATGATCGCTGCTGAGGTACCGCTGACCCGGGTGCGGATACATATGGTGAACACCGGGCGGATCC
TGATCGCCGATGTGCCGGTCGCCGACGGTATGCCGCGGGTTGAGGGCGACTGCAGTATCGACGGCGTACCGGGCAGTGGC
GCCCGCATCGATCTCGACTGGGCCGACTGCGGCGGGGAGACCACCGGCGCGCTGCTGCCCAGCGGAGCACCCTGTGACAC
CATTGAGGCCGGCGGACGTGAGTATCAGGTCTCCATCATTGATGCGGGCAACGTCACGGTGTTTATCGATGCCGGGGCGC
TGGGGCTGCGCGGTACGGAGACCCCGGCCGAAATCAGCGGCGATGCGCAGCGGATGGCACTGATCGAAGAGATCCGCGCC
AAGGCCGCGCAGCGGATTGGACTGATCGATGACTGGCGCGAGGCGGCGCAGCAGATCCCCTATGCGCCCTTTTTTAGCAT
TGTCAGTTCACCGCGCACGCATGATACCTTTAACGGTAAGCACATTGAGGCGGACCAGTGCGACATTGTCTCACGCCTGG
TATTTATGCAGGCGCTGCACAAGGCCCACCCGGTGACGGGTACCGTCGCGCTGGGCGCCGCCGCCCGCATTCCCGGCACC
CTGGTATACCAACGGATGGCACCGGCGGCCCACGCCGGCGATCGCCTGCGCATCGGCCATCCCAGCGGGGTGATCGTGAC
CGACTGCGCCGTCGGACGACAGGGGGAGACATACCAGCTGCGTAAGTCGCGGGTGACCCGCACCGCCCGGATCCTGCTGG
ACGGACAATTTTACCTGCGAAACAGCCTGTTGGCTCCGACGTCCGACGGGAAGTAG

Upstream 100 bases:

>100_bases
CAGCAGGAGATCTACCCCTGCACCCGTCTGCCGGATAACATTATGGCGATGATCCGCGCCGGCGGACTGGTAAACCATCT
CAGACAAACCGGAGGTTGTG

Downstream 100 bases:

>100_bases
CGCCTCACGCCGGGCTGAGCCACGGGGGCCGCTCACGCGGCCCCTCTGCTGGCGGGCTCACCCGGTACCGTTGACCAAGG
AGTTTCCATGAGCGCCGAAT

Product: hypothetical protein

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MQTEVTALPVAIVRGGTSKGIFIMKNALPADPALRDRVILSVFGSPDLRQIDGLGGADPLTSKLAIIAPASRPDADVDYI
FGQVSLSEAFIDYGGNCGNISAAVGAFAIQKGMIAAEVPLTRVRIHMVNTGRILIADVPVADGMPRVEGDCSIDGVPGSG
ARIDLDWADCGGETTGALLPSGAPCDTIEAGGREYQVSIIDAGNVTVFIDAGALGLRGTETPAEISGDAQRMALIEEIRA
KAAQRIGLIDDWREAAQQIPYAPFFSIVSSPRTHDTFNGKHIEADQCDIVSRLVFMQALHKAHPVTGTVALGAAARIPGT
LVYQRMAPAAHAGDRLRIGHPSGVIVTDCAVGRQGETYQLRKSRVTRTARILLDGQFYLRNSLLAPTSDGK

Sequences:

>Translated_391_residues
MQTEVTALPVAIVRGGTSKGIFIMKNALPADPALRDRVILSVFGSPDLRQIDGLGGADPLTSKLAIIAPASRPDADVDYI
FGQVSLSEAFIDYGGNCGNISAAVGAFAIQKGMIAAEVPLTRVRIHMVNTGRILIADVPVADGMPRVEGDCSIDGVPGSG
ARIDLDWADCGGETTGALLPSGAPCDTIEAGGREYQVSIIDAGNVTVFIDAGALGLRGTETPAEISGDAQRMALIEEIRA
KAAQRIGLIDDWREAAQQIPYAPFFSIVSSPRTHDTFNGKHIEADQCDIVSRLVFMQALHKAHPVTGTVALGAAARIPGT
LVYQRMAPAAHAGDRLRIGHPSGVIVTDCAVGRQGETYQLRKSRVTRTARILLDGQFYLRNSLLAPTSDGK
>Mature_391_residues
MQTEVTALPVAIVRGGTSKGIFIMKNALPADPALRDRVILSVFGSPDLRQIDGLGGADPLTSKLAIIAPASRPDADVDYI
FGQVSLSEAFIDYGGNCGNISAAVGAFAIQKGMIAAEVPLTRVRIHMVNTGRILIADVPVADGMPRVEGDCSIDGVPGSG
ARIDLDWADCGGETTGALLPSGAPCDTIEAGGREYQVSIIDAGNVTVFIDAGALGLRGTETPAEISGDAQRMALIEEIRA
KAAQRIGLIDDWREAAQQIPYAPFFSIVSSPRTHDTFNGKHIEADQCDIVSRLVFMQALHKAHPVTGTVALGAAARIPGT
LVYQRMAPAAHAGDRLRIGHPSGVIVTDCAVGRQGETYQLRKSRVTRTARILLDGQFYLRNSLLAPTSDGK

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=372, Percent_Identity=36.0215053763441, Blast_Score=222, Evalue=4e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 41218; Mature: 41218

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTEVTALPVAIVRGGTSKGIFIMKNALPADPALRDRVILSVFGSPDLRQIDGLGGADPL
CCCCCEEEEEEEEECCCCCCEEEEECCCCCCCCHHCEEEEEECCCCCHHHHCCCCCCCCC
TSKLAIIAPASRPDADVDYIFGQVSLSEAFIDYGGNCGNISAAVGAFAIQKGMIAAEVPL
CCCEEEEECCCCCCCCCEEEEEEEEHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEECCCE
TRVRIHMVNTGRILIADVPVADGMPRVEGDCSIDGVPGSGARIDLDWADCGGETTGALLP
EEEEEEEEECCEEEEEECCCCCCCCCCCCCCEECCCCCCCCEEEEEHHHCCCCCCCCCCC
SGAPCDTIEAGGREYQVSIIDAGNVTVFIDAGALGLRGTETPAEISGDAQRMALIEEIRA
CCCCCCHHHCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCCHHCCCCHHHHHHHHHHHH
KAAQRIGLIDDWREAAQQIPYAPFFSIVSSPRTHDTFNGKHIEADQCDIVSRLVFMQALH
HHHHHCCCHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHH
KAHPVTGTVALGAAARIPGTLVYQRMAPAAHAGDRLRIGHPSGVIVTDCAVGRQGETYQL
HCCCCCEEEEECCHHHCCHHHHHHHHCCCCCCCCEEEECCCCCEEEEEECCCCCCCCHHH
RKSRVTRTARILLDGQFYLRNSLLAPTSDGK
HHHHHCEEEEEEECCCCHHHCCCCCCCCCCC
>Mature Secondary Structure
MQTEVTALPVAIVRGGTSKGIFIMKNALPADPALRDRVILSVFGSPDLRQIDGLGGADPL
CCCCCEEEEEEEEECCCCCCEEEEECCCCCCCCHHCEEEEEECCCCCHHHHCCCCCCCCC
TSKLAIIAPASRPDADVDYIFGQVSLSEAFIDYGGNCGNISAAVGAFAIQKGMIAAEVPL
CCCEEEEECCCCCCCCCEEEEEEEEHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEECCCE
TRVRIHMVNTGRILIADVPVADGMPRVEGDCSIDGVPGSGARIDLDWADCGGETTGALLP
EEEEEEEEECCEEEEEECCCCCCCCCCCCCCEECCCCCCCCEEEEEHHHCCCCCCCCCCC
SGAPCDTIEAGGREYQVSIIDAGNVTVFIDAGALGLRGTETPAEISGDAQRMALIEEIRA
CCCCCCHHHCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCCHHCCCCHHHHHHHHHHHH
KAAQRIGLIDDWREAAQQIPYAPFFSIVSSPRTHDTFNGKHIEADQCDIVSRLVFMQALH
HHHHHCCCHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHH
KAHPVTGTVALGAAARIPGTLVYQRMAPAAHAGDRLRIGHPSGVIVTDCAVGRQGETYQL
HCCCCCEEEEECCHHHCCHHHHHHHHCCCCCCCCEEEECCCCCEEEEEECCCCCCCCHHH
RKSRVTRTARILLDGQFYLRNSLLAPTSDGK
HHHHHCEEEEEEECCCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA