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The map label for this gene is amtB [C]

Identifier: 238919951

GI number: 238919951

Start: 1977255

End: 1977824

Strand: Reverse

Name: amtB [C]

Synonym: NT01EI_2055

Alternate gene names: 238919951

Gene position: 1977824-1977255 (Counterclockwise)

Preceding gene: 238919952

Following gene: 238919945

Centisome position: 51.88

GC content: 47.72

Gene sequence:

>570_bases
ATGCTGCAAAATTTTATCTGTATGGGGGTGGTAGCAGTTATTTGGATATTCGGTGGGTTTAGCTTGGTATTCGGACAGGA
TGTGGGGGGAGTGATCGGTAACTTTTTTGATTACTTTGGAATGCTACATATCGGCGTTTCGGTGAATAAAGCGTGGGCGC
CAAATATTCCATTTATCTTGTTTTTTGCCTATCAAATGATGTTTGCCATCATTACGCCAGCGTTGATCAGTGGTGCTATC
GTTGGGCGTGTCAAGTTCTCCGCCTATGTTAAATTTGTTTTCTTATGGGTGATCTTCGTCTATATCCCCGTTGCTCACTG
GGTCTGGGGAGGCGGTTTCTTAGAGCAAATCGGCGTGGTTGATTTTGCAGGCGGTATCGTGGTGCATGTGACCGCGGGGT
TCTCTGCGCTCGCCGCGGCGTTGTTCATCGGTAAACGGGTTGACTCGGTTATAAAATCAGAGAAGCCAGTGAGCTTGCCG
ATTGTGGCTTGTGGGGCCGGCTTACTGTGGTTTGGTTGGTTTGGATTTAGAGCCGCTAACAAAACCGTTCAACAAAGCGC
AAGCAGATAA

Upstream 100 bases:

>100_bases
CGCATTCATAATTTTATGTACGGCAATGGTTTGCCTGATGACACCAGGATTAGCGCTATTTTATGGCGGTTTGGTTAGCG
AGCGGAATATAATTTCTGTT

Downstream 100 bases:

>100_bases
CAGCACAAGCCAGCGTCAGCAAGGCAAGATGGGTATCCAGCCGCCGTTCGAAGCGAATACGCAGCTTGGACCCTGTACAC
GATTCTGTGTAAATGCCTTT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MLQNFICMGVVAVIWIFGGFSLVFGQDVGGVIGNFFDYFGMLHIGVSVNKAWAPNIPFILFFAYQMMFAIITPALISGAI
VGRVKFSAYVKFVFLWVIFVYIPVAHWVWGGGFLEQIGVVDFAGGIVVHVTAGFSALAAALFIGKRVDSVIKSEKPVSLP
IVACGAGLLWFGWFGFRAANKTVQQSASR

Sequences:

>Translated_189_residues
MLQNFICMGVVAVIWIFGGFSLVFGQDVGGVIGNFFDYFGMLHIGVSVNKAWAPNIPFILFFAYQMMFAIITPALISGAI
VGRVKFSAYVKFVFLWVIFVYIPVAHWVWGGGFLEQIGVVDFAGGIVVHVTAGFSALAAALFIGKRVDSVIKSEKPVSLP
IVACGAGLLWFGWFGFRAANKTVQQSASR
>Mature_189_residues
MLQNFICMGVVAVIWIFGGFSLVFGQDVGGVIGNFFDYFGMLHIGVSVNKAWAPNIPFILFFAYQMMFAIITPALISGAI
VGRVKFSAYVKFVFLWVIFVYIPVAHWVWGGGFLEQIGVVDFAGGIVVHVTAGFSALAAALFIGKRVDSVIKSEKPVSLP
IVACGAGLLWFGWFGFRAANKTVQQSASR

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=180, Percent_Identity=40, Blast_Score=121, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI133931100, Length=173, Percent_Identity=30.0578034682081, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI32564042, Length=123, Percent_Identity=34.9593495934959, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324187, Length=182, Percent_Identity=39.5604395604396, Blast_Score=108, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6325396, Length=170, Percent_Identity=30.5882352941176, Blast_Score=92, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6321559, Length=171, Percent_Identity=31.5789473684211, Blast_Score=90, Evalue=3e-19,
Organism=Drosophila melanogaster, GI161078314, Length=170, Percent_Identity=35.8823529411765, Blast_Score=71, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047
- InterPro:   IPR002229 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 20529; Mature: 20529

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQNFICMGVVAVIWIFGGFSLVFGQDVGGVIGNFFDYFGMLHIGVSVNKAWAPNIPFIL
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
FFAYQMMFAIITPALISGAIVGRVKFSAYVKFVFLWVIFVYIPVAHWVWGGGFLEQIGVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCHH
DFAGGIVVHVTAGFSALAAALFIGKRVDSVIKSEKPVSLPIVACGAGLLWFGWFGFRAAN
HCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
KTVQQSASR
HHHHHHCCC
>Mature Secondary Structure
MLQNFICMGVVAVIWIFGGFSLVFGQDVGGVIGNFFDYFGMLHIGVSVNKAWAPNIPFIL
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
FFAYQMMFAIITPALISGAIVGRVKFSAYVKFVFLWVIFVYIPVAHWVWGGGFLEQIGVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCHH
DFAGGIVVHVTAGFSALAAALFIGKRVDSVIKSEKPVSLPIVACGAGLLWFGWFGFRAAN
HCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
KTVQQSASR
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]