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The map label for this gene is iolJ [H]

Identifier: 238919659

GI number: 238919659

Start: 1708357

End: 1709235

Strand: Reverse

Name: iolJ [H]

Synonym: NT01EI_1760

Alternate gene names: 238919659

Gene position: 1709235-1708357 (Counterclockwise)

Preceding gene: 238919660

Following gene: 238919658

Centisome position: 44.83

GC content: 54.38

Gene sequence:

>879_bases
ATGAGTTTGATAAATCTGGCCAAGATGCTGAAAACCGCTAAAAAAGAACATTTTGCTGTTGGTGCGTTCAATGCCATCGA
TAATCACTTCGTCGATGCAATTTTTGCCGCTGCCGATCAAAATCATTCCCCTATCATTCTCAATTTTGCTGAAGTGCATA
GCCGTCTGGTCAACCTGGAAGACATGGCCGATTACGTATGCTTCAAAGCCCGCCGCGCGAATGTGCCGGTGACGTTAAAC
CTGGATCATGGGCTGACACTGGACGCAGTAACTCGTGCCATCAACAGCGGCTTTACGTCCATCATGTTTGATGGCTCCCA
TCTTGATTACGAAGACAACGTCCGCCAGACGGCGGACGTTGTCGACATATGTCGTGAGCTAAATATCTCCGTCGAAGGCG
AGCTGGGTGCCGTCGGCGGCGATGAAGGCGGCGCTCTGGAAGGATCGGCGGATGCCGCACAGTATACCGATATCGACCAG
GCGCACGACTTTGTCTCCCGCACCGGTATTAACGCGTTGGCCGTCGCCATCGGCAACAGCCACGGCCGCTACAAAGGGGT
GCCGAAACTGGATTTTGCCCGCCTGGACGCACTGGCAAATTGCGTCGATGTGCCGCTGGTGCTACACGGTGGCTCCGGGC
TTTCCGCGCAAGATTTCCGCCGGGTCATCGAACTCGGTATCGCCAAGATTAACTTCTATACCGGCATGTCGCAGGTTGCT
CTGCATACGCTAGAAACCCGGCTGCATGACACCGCATTGAGCAACAAATACGACCACTACCTGCTGCTGATGAAAGAGGT
CGAGCATGGCGTTAGCACCACCGTCGCAGAGCAAATGGCCATCTTCGGCAGTCTGGGCAAGGCAGACGGCTATGCATAA

Upstream 100 bases:

>100_bases
CCGTTTCCTGGTTCCGCTTTCCCGCTTTGCCGAAATTGAAGAAGATGAACCGGTGAAATGGGTTCTTTGCAATGAATATC
AATTGATTAAGGGATAAGTA

Downstream 100 bases:

>100_bases
ACGTCGGGGGATCATTGCGATCGGCAATTTGATAGTCGATCAGTCGCTGCGTTGCACGGAATATCCACAGGAGTCGATGC
TGACGCAAATTACCCACGTC

Product: hypothetical protein

Products: NA

Alternate protein names: DKGP aldolase [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MSLINLAKMLKTAKKEHFAVGAFNAIDNHFVDAIFAAADQNHSPIILNFAEVHSRLVNLEDMADYVCFKARRANVPVTLN
LDHGLTLDAVTRAINSGFTSIMFDGSHLDYEDNVRQTADVVDICRELNISVEGELGAVGGDEGGALEGSADAAQYTDIDQ
AHDFVSRTGINALAVAIGNSHGRYKGVPKLDFARLDALANCVDVPLVLHGGSGLSAQDFRRVIELGIAKINFYTGMSQVA
LHTLETRLHDTALSNKYDHYLLLMKEVEHGVSTTVAEQMAIFGSLGKADGYA

Sequences:

>Translated_292_residues
MSLINLAKMLKTAKKEHFAVGAFNAIDNHFVDAIFAAADQNHSPIILNFAEVHSRLVNLEDMADYVCFKARRANVPVTLN
LDHGLTLDAVTRAINSGFTSIMFDGSHLDYEDNVRQTADVVDICRELNISVEGELGAVGGDEGGALEGSADAAQYTDIDQ
AHDFVSRTGINALAVAIGNSHGRYKGVPKLDFARLDALANCVDVPLVLHGGSGLSAQDFRRVIELGIAKINFYTGMSQVA
LHTLETRLHDTALSNKYDHYLLLMKEVEHGVSTTVAEQMAIFGSLGKADGYA
>Mature_291_residues
SLINLAKMLKTAKKEHFAVGAFNAIDNHFVDAIFAAADQNHSPIILNFAEVHSRLVNLEDMADYVCFKARRANVPVTLNL
DHGLTLDAVTRAINSGFTSIMFDGSHLDYEDNVRQTADVVDICRELNISVEGELGAVGGDEGGALEGSADAAQYTDIDQA
HDFVSRTGINALAVAIGNSHGRYKGVPKLDFARLDALANCVDVPLVLHGGSGLSAQDFRRVIELGIAKINFYTGMSQVAL
HTLETRLHDTALSNKYDHYLLLMKEVEHGVSTTVAEQMAIFGSLGKADGYA

Specific function: Produces dihydroxyacetone phosphate (DHAP or glycerone phosphate) and malonic semialdehyde (MSA or 3-oxopropanoate) from 6-phospho-5-dehydro-2-deoxy-D-gluconate (DKGP) [H]

COG id: COG0191

COG function: function code G; Fructose/tagatose bisphosphate aldolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class II fructose-bisphosphate aldolase family. IolJ subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788072, Length=277, Percent_Identity=37.5451263537906, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI87082041, Length=288, Percent_Identity=34.0277777777778, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1789526, Length=271, Percent_Identity=36.1623616236162, Blast_Score=168, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011289
- InterPro:   IPR000771 [H]

Pfam domain/function: PF01116 F_bP_aldolase [H]

EC number: =4.1.2.29 [H]

Molecular weight: Translated: 31534; Mature: 31403

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00806 ALDOLASE_CLASS_II_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLINLAKMLKTAKKEHFAVGAFNAIDNHFVDAIFAAADQNHSPIILNFAEVHSRLVNLE
CCHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHCCHH
DMADYVCFKARRANVPVTLNLDHGLTLDAVTRAINSGFTSIMFDGSHLDYEDNVRQTADV
HHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHH
VDICRELNISVEGELGAVGGDEGGALEGSADAAQYTDIDQAHDFVSRTGINALAVAIGNS
HHHHHHCCCEECCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHCCCCEEEEEECCC
HGRYKGVPKLDFARLDALANCVDVPLVLHGGSGLSAQDFRRVIELGIAKINFYTGMSQVA
CCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCHHHHH
LHTLETRLHDTALSNKYDHYLLLMKEVEHGVSTTVAEQMAIFGSLGKADGYA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SLINLAKMLKTAKKEHFAVGAFNAIDNHFVDAIFAAADQNHSPIILNFAEVHSRLVNLE
CHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHCCHH
DMADYVCFKARRANVPVTLNLDHGLTLDAVTRAINSGFTSIMFDGSHLDYEDNVRQTADV
HHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHH
VDICRELNISVEGELGAVGGDEGGALEGSADAAQYTDIDQAHDFVSRTGINALAVAIGNS
HHHHHHCCCEECCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHCCCCEEEEEECCC
HGRYKGVPKLDFARLDALANCVDVPLVLHGGSGLSAQDFRRVIELGIAKINFYTGMSQVA
CCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCHHHHH
LHTLETRLHDTALSNKYDHYLLLMKEVEHGVSTTVAEQMAIFGSLGKADGYA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]