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The map label for this gene is pncA [H]

Identifier: 238919548

GI number: 238919548

Start: 1605695

End: 1606330

Strand: Reverse

Name: pncA [H]

Synonym: NT01EI_1644

Alternate gene names: 238919548

Gene position: 1606330-1605695 (Counterclockwise)

Preceding gene: 238919549

Following gene: 238919545

Centisome position: 42.14

GC content: 63.05

Gene sequence:

>636_bases
ATGTCCGCTGCACTTTTGCTGATCGATCTACAGAATGATTTTTGCCCCCATGGCGCGCTGGCGGTTCCCCAGGGCGATGA
GGTCATCCCTGTCGCCCTGCAGGCGGTCGCCATGGCGCGTCGGGATGGCACCCCAGTAATCGCAACCCAGGATTGGCACC
CGGCGGAACACGGCAGCTTCGCCAGCCGCAGCGGCGGACAGATGGGTGAGGTCGGCAAGCTCAATGGGCTGCCCCAGGTC
TGGTGGCCTGACCACTGCGTGCAGAACACCATCGGTGCCGCGTTTCACCCAGCCCTGCGCAATACCGCCTTCGATTTTGT
GGTGCATAAGGGAACCGATGCTGCGGTAGACAGCTATAGCGCCTTTTTCGATAACGGGCAGCGCGGTGCCACCGCGCTGG
ATGCCTGGCTGCGCCAGCACGGTATCACCCGCCTGTGGCTACTGGGTCTGGCAACAGATTACTGTGTGAAGTTTTCGGTG
TTGGACGCCCTGCGCCTGGGCTATCAGGTCAATATCATTAGCGACGGCTGCCGCGGCGTGGATCTCACTCCCGGCGATAG
CGAACGCGCGCTGGCCGGGATGGTAAAAGCCGGGGCAGCCCTAACCCCCCTCACCACGCTCAGCCACCTGCGCTGA

Upstream 100 bases:

>100_bases
GATCTGCGCGGAGAGCTGAGCGAGACCCCCTAAGCGTAATATCTCACCGCGACAACGTCGGGAGAGACAACCTAGCCATA
CAACCACGGGAGGACGACGG

Downstream 100 bases:

>100_bases
GCGCGCTCCCGCACGGCCGCGCCCTACTTCGCCAACGGCGCGGCAAACAGTGCTTTCAGCGCTTGCTTGCTTTTCATCTC
TATCTCCCCGCGGGTATTCA

Product: nicotinamidase/pyrazinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MSAALLLIDLQNDFCPHGALAVPQGDEVIPVALQAVAMARRDGTPVIATQDWHPAEHGSFASRSGGQMGEVGKLNGLPQV
WWPDHCVQNTIGAAFHPALRNTAFDFVVHKGTDAAVDSYSAFFDNGQRGATALDAWLRQHGITRLWLLGLATDYCVKFSV
LDALRLGYQVNIISDGCRGVDLTPGDSERALAGMVKAGAALTPLTTLSHLR

Sequences:

>Translated_211_residues
MSAALLLIDLQNDFCPHGALAVPQGDEVIPVALQAVAMARRDGTPVIATQDWHPAEHGSFASRSGGQMGEVGKLNGLPQV
WWPDHCVQNTIGAAFHPALRNTAFDFVVHKGTDAAVDSYSAFFDNGQRGATALDAWLRQHGITRLWLLGLATDYCVKFSV
LDALRLGYQVNIISDGCRGVDLTPGDSERALAGMVKAGAALTPLTTLSHLR
>Mature_210_residues
SAALLLIDLQNDFCPHGALAVPQGDEVIPVALQAVAMARRDGTPVIATQDWHPAEHGSFASRSGGQMGEVGKLNGLPQVW
WPDHCVQNTIGAAFHPALRNTAFDFVVHKGTDAAVDSYSAFFDNGQRGATALDAWLRQHGITRLWLLGLATDYCVKFSVL
DALRLGYQVNIISDGCRGVDLTPGDSERALAGMVKAGAALTPLTTLSHLR

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=201, Percent_Identity=59.7014925373134, Blast_Score=246, Evalue=9e-67,
Organism=Caenorhabditis elegans, GI193206735, Length=225, Percent_Identity=32.4444444444444, Blast_Score=104, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17543130, Length=225, Percent_Identity=32.4444444444444, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71999339, Length=201, Percent_Identity=31.8407960199005, Blast_Score=90, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6321401, Length=195, Percent_Identity=30.7692307692308, Blast_Score=105, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24648247, Length=202, Percent_Identity=34.6534653465347, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 22543; Mature: 22412

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAALLLIDLQNDFCPHGALAVPQGDEVIPVALQAVAMARRDGTPVIATQDWHPAEHGSF
CCCEEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
ASRSGGQMGEVGKLNGLPQVWWPDHCVQNTIGAAFHPALRNTAFDFVVHKGTDAAVDSYS
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHH
AFFDNGQRGATALDAWLRQHGITRLWLLGLATDYCVKFSVLDALRLGYQVNIISDGCRGV
HHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCC
DLTPGDSERALAGMVKAGAALTPLTTLSHLR
CCCCCCCHHHHHHHHHCCCCCCHHHHHHHCC
>Mature Secondary Structure 
SAALLLIDLQNDFCPHGALAVPQGDEVIPVALQAVAMARRDGTPVIATQDWHPAEHGSF
CCEEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
ASRSGGQMGEVGKLNGLPQVWWPDHCVQNTIGAAFHPALRNTAFDFVVHKGTDAAVDSYS
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHH
AFFDNGQRGATALDAWLRQHGITRLWLLGLATDYCVKFSVLDALRLGYQVNIISDGCRGV
HHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCC
DLTPGDSERALAGMVKAGAALTPLTTLSHLR
CCCCCCCHHHHHHHHHCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]