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The map label for this gene is ecoRIIM [H]

Identifier: 238919389

GI number: 238919389

Start: 1457274

End: 1458689

Strand: Reverse

Name: ecoRIIM [H]

Synonym: NT01EI_1483

Alternate gene names: 238919389

Gene position: 1458689-1457274 (Counterclockwise)

Preceding gene: 238919391

Following gene: 238919383

Centisome position: 38.26

GC content: 39.41

Gene sequence:

>1416_bases
ATGTCAGAATTTGATCTATTTGCTCAGGAATTGCTCGAAAAAGCAAAAACAGAAGAAAAACAGCAGCAGGTACGTGACAG
AAAGCTTATTGATCGGGTTCTTGAAATTTATGATCAAAAATATGTAGCTGAATTACTTAGAAAAGTTGGGAAAAATGAGT
GGAGCCGTGAAACGTTAAACCGCTGGGTGAACGAAAAATGTGAGCCTAAATCACTCACAGTTGCAGAAGAAGCGCTGCTG
CGAAAAATGTTGCCGGAACGTCCTGCCCACGAGCATAAATTTAGGTTTATTGACCTTTTTGCCGGAATTGGCGGGATCCG
AAAAGGCTTTGAATCCATTGGCGGGCAATGTGTTTTTACCAGTGAGTGGAACAAGGAGGCCGTCCGGACCTATAAAGCCA
ATTGGTATAATGATGAAGATCTGCATACATTTAATATGGACATTAGAGAAATTACTCTAAGCGCAGAAAGTGATATATCT
GAACATGATGCCTATAAGAATATAAATCAGCATATTCCAGACCACGATGTACTATTAGCTGGATTTCCCTGTCAACCTTT
CAGTCTCGCTGGTGTGAGTAAAAAAAACTCGCTTGGGCGCGCTCACGGTTTCGAATGTGAAGCACAAGGTACACTATTCT
TCGATGTCGCAAGGATAATCAAAGCAAAACGACCAGCAATTTTCGTCCTTGAAAATGTTAAGAATTTAAAAAGTCATGAC
AAGGGGAAAACATTTAAGGTTATAATGGATACGCTTGATGAGTTGGGATATGAAGTTGCCGATTCAGACGCTAATGGAAA
ACCAGATCCTAAAATCATTGATGGGAAGCATTTTTTACCACAACACCGGGAGCGCATAGTTCTTGTTGGTTTTCGTCGTG
ACCTCAATATACATCACGACTTTACCCTACAAAAAATCCAGCAGTTCTATCCTGAAAAAAAACCAACATTTGGTGAGTTA
TTAGAACCAGCCGTTGACAGCAAATACATATTAAGCCCAAAATTATGGGAATATCTATATAACTATGCAAAGAAACATGC
AGAAAAAGGGAATGGATTTGGCTTTGGTTTAGTTGATCCAAAAAATAAAGAAAGCATTGCCAGGACATTGTCTGCAAGAT
ATCATAAGGATGGTTCTGAAATACTTATTGACAGAGGATGGGATAAGGCTATAGGAGAGATTAACTTTACCGATCCGAAG
AATCAAGAGAGAAGACCAAGAAGGCTGACACCCAGAGAGTGCGCTAGATTGATGGGTTTTGAGAAACAGAACGAAAAACA
ATTCAGAATTCCTGTTTCTGACACTCAGGCTTATCGCCAATTTGGCAACTCAGTAGTAGTTCCTGTATTTGAAGCAGTAG
CAAAACTACTGGCTCCATATATAGATAAAGCCATGCAGGAAAAATATAATAAATAA

Upstream 100 bases:

>100_bases
ATTCATCTCCTCCAAATATACTGGTTAAATATCCAGAACACTCATTCCATCTGGGTCAAATGAGTGATACTATATCACCC
ATAAACCCCAAGGAGGCGTT

Downstream 100 bases:

>100_bases
TATATTTGATTGAATACCAGAAGACTTATGGTATTCAATCAATCATTTTTAAGAGTACAGTTTTTTAGTATCAAAAATAA
AATCATTCAATGAAATTAAA

Product: DNA cytosine methylase

Products: NA

Alternate protein names: M.EcoRII; Cytosine-specific methyltransferase EcoRII [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MSEFDLFAQELLEKAKTEEKQQQVRDRKLIDRVLEIYDQKYVAELLRKVGKNEWSRETLNRWVNEKCEPKSLTVAEEALL
RKMLPERPAHEHKFRFIDLFAGIGGIRKGFESIGGQCVFTSEWNKEAVRTYKANWYNDEDLHTFNMDIREITLSAESDIS
EHDAYKNINQHIPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECEAQGTLFFDVARIIKAKRPAIFVLENVKNLKSHD
KGKTFKVIMDTLDELGYEVADSDANGKPDPKIIDGKHFLPQHRERIVLVGFRRDLNIHHDFTLQKIQQFYPEKKPTFGEL
LEPAVDSKYILSPKLWEYLYNYAKKHAEKGNGFGFGLVDPKNKESIARTLSARYHKDGSEILIDRGWDKAIGEINFTDPK
NQERRPRRLTPRECARLMGFEKQNEKQFRIPVSDTQAYRQFGNSVVVPVFEAVAKLLAPYIDKAMQEKYNK

Sequences:

>Translated_471_residues
MSEFDLFAQELLEKAKTEEKQQQVRDRKLIDRVLEIYDQKYVAELLRKVGKNEWSRETLNRWVNEKCEPKSLTVAEEALL
RKMLPERPAHEHKFRFIDLFAGIGGIRKGFESIGGQCVFTSEWNKEAVRTYKANWYNDEDLHTFNMDIREITLSAESDIS
EHDAYKNINQHIPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECEAQGTLFFDVARIIKAKRPAIFVLENVKNLKSHD
KGKTFKVIMDTLDELGYEVADSDANGKPDPKIIDGKHFLPQHRERIVLVGFRRDLNIHHDFTLQKIQQFYPEKKPTFGEL
LEPAVDSKYILSPKLWEYLYNYAKKHAEKGNGFGFGLVDPKNKESIARTLSARYHKDGSEILIDRGWDKAIGEINFTDPK
NQERRPRRLTPRECARLMGFEKQNEKQFRIPVSDTQAYRQFGNSVVVPVFEAVAKLLAPYIDKAMQEKYNK
>Mature_470_residues
SEFDLFAQELLEKAKTEEKQQQVRDRKLIDRVLEIYDQKYVAELLRKVGKNEWSRETLNRWVNEKCEPKSLTVAEEALLR
KMLPERPAHEHKFRFIDLFAGIGGIRKGFESIGGQCVFTSEWNKEAVRTYKANWYNDEDLHTFNMDIREITLSAESDISE
HDAYKNINQHIPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECEAQGTLFFDVARIIKAKRPAIFVLENVKNLKSHDK
GKTFKVIMDTLDELGYEVADSDANGKPDPKIIDGKHFLPQHRERIVLVGFRRDLNIHHDFTLQKIQQFYPEKKPTFGELL
EPAVDSKYILSPKLWEYLYNYAKKHAEKGNGFGFGLVDPKNKESIARTLSARYHKDGSEILIDRGWDKAIGEINFTDPKN
QERRPRRLTPRECARLMGFEKQNEKQFRIPVSDTQAYRQFGNSVVVPVFEAVAKLLAPYIDKAMQEKYNK

Specific function: This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the EcoRII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=439, Percent_Identity=67.8815489749431, Blast_Score=605, Evalue=1e-174,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 54569; Mature: 54438

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEFDLFAQELLEKAKTEEKQQQVRDRKLIDRVLEIYDQKYVAELLRKVGKNEWSRETLN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
RWVNEKCEPKSLTVAEEALLRKMLPERPAHEHKFRFIDLFAGIGGIRKGFESIGGQCVFT
HHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
SEWNKEAVRTYKANWYNDEDLHTFNMDIREITLSAESDISEHDAYKNINQHIPDHDVLLA
CCCCHHHHHHHHCCCCCCCCCEEECCCHHHEEECCCCCCHHHHHHHHHHHCCCCCCEEEE
GFPCQPFSLAGVSKKNSLGRAHGFECEAQGTLFFDVARIIKAKRPAIFVLENVKNLKSHD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHCC
KGKTFKVIMDTLDELGYEVADSDANGKPDPKIIDGKHFLPQHRERIVLVGFRRDLNIHHD
CCCHHHHHHHHHHHCCCEEECCCCCCCCCCCEECCCCCCCCCCCEEEEEEEECCCCCCCH
FTLQKIQQFYPEKKPTFGELLEPAVDSKYILSPKLWEYLYNYAKKHAEKGNGFGFGLVDP
HHHHHHHHHCCCCCCCHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHCCCCCCEECCCCC
KNKESIARTLSARYHKDGSEILIDRGWDKAIGEINFTDPKNQERRPRRLTPRECARLMGF
CCHHHHHHHHHHHHCCCCCEEEEECCCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCC
EKQNEKQFRIPVSDTQAYRQFGNSVVVPVFEAVAKLLAPYIDKAMQEKYNK
CCCCCCEEECCCCHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEFDLFAQELLEKAKTEEKQQQVRDRKLIDRVLEIYDQKYVAELLRKVGKNEWSRETLN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
RWVNEKCEPKSLTVAEEALLRKMLPERPAHEHKFRFIDLFAGIGGIRKGFESIGGQCVFT
HHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
SEWNKEAVRTYKANWYNDEDLHTFNMDIREITLSAESDISEHDAYKNINQHIPDHDVLLA
CCCCHHHHHHHHCCCCCCCCCEEECCCHHHEEECCCCCCHHHHHHHHHHHCCCCCCEEEE
GFPCQPFSLAGVSKKNSLGRAHGFECEAQGTLFFDVARIIKAKRPAIFVLENVKNLKSHD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHCC
KGKTFKVIMDTLDELGYEVADSDANGKPDPKIIDGKHFLPQHRERIVLVGFRRDLNIHHD
CCCHHHHHHHHHHHCCCEEECCCCCCCCCCCEECCCCCCCCCCCEEEEEEEECCCCCCCH
FTLQKIQQFYPEKKPTFGELLEPAVDSKYILSPKLWEYLYNYAKKHAEKGNGFGFGLVDP
HHHHHHHHHCCCCCCCHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHCCCCCCEECCCCC
KNKESIARTLSARYHKDGSEILIDRGWDKAIGEINFTDPKNQERRPRRLTPRECARLMGF
CCHHHHHHHHHHHHCCCCCEEEEECCCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCC
EKQNEKQFRIPVSDTQAYRQFGNSVVVPVFEAVAKLLAPYIDKAMQEKYNK
CCCCCCEEECCCCHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3029675 [H]