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The map label for this gene is iadA [H]

Identifier: 238919383

GI number: 238919383

Start: 1451208

End: 1452356

Strand: Reverse

Name: iadA [H]

Synonym: NT01EI_1477

Alternate gene names: 238919383

Gene position: 1452356-1451208 (Counterclockwise)

Preceding gene: 238919389

Following gene: 238919382

Centisome position: 38.1

GC content: 65.19

Gene sequence:

>1149_bases
ATGTTTACCCTGCTTAAAGATGCCGACGTCTATCAGCCACAGCCCCTGGGGCGCCGGGACGTATTGCTGTGTGGTGCGAA
GATCGTCGCCATCGGGGAGAACCTGCAGGTCAGCGCGCTGCCCGGGCTGCGCGAGGTTATCGACTGTCGCGGTCAGATCC
TGGCCCCCGGCCTCATCGACCAGCATGTGCATCTGATCGGCGGCGGCGGCGAGGGCGGCTTTGCCAGCCGGACCCCCGCG
GTCTCCCTGTCGCGACTGATCCGCGCCGCGACCACCACCGTCGTCGGGGTGCTCGGCACCGACGGTATCTCCCGTACCCC
CAAAGATCTGTACGCCAAAATGCAGGCGCTGAACGCGGAGGGGATCAGCGCCTATATGCACACCGGATCTTACGAGCTCC
CCACCCGGACCATCACCGGCACCATTCGCGATGATATCGCCTTTATTGAGCGGGTGCTGGGGGTGAAGATAGCGCTGGCC
GATCACCGCGGTTCGTTCCCGACGACCCAGGAGCTGCTGCGTATCGTCTCCGATATCCGCGTTGCCGCGCTGCTGGCGGG
CAAACGTGGGCTGCTGCACATCCATCTGGGCGCGCTGCCCGACGCTTTTGCCCAGATCAATGCGCTGCTGGCGCACGGTC
TGCCTATCCAGCATATCTCGCCGACCCACGTGGCGCGCACCGAAGCGCTGTTCGCTGACGCCATCGCCTTTGCCCGCGCC
GGAGGCCATATCGATATGACCTCCGGCGGCAGCCGCTTTGTCGCGCCAGCCTGCGCCGCCCGCCTGGCGCTGGAGGCCGG
TGTCAGCCCGGATCGGCTCACCATCAGCTCCGATGGCAACGGCAGCGTGCCGCGCTTTGATGCGCAGGGCAGCATTATCG
GGCTGGATGCGGCACCGCTGGACGGCAACCTGCAGCTGCTGCCACAGCTGATTGACCAGCGCATTCCCCTGCCGCAGGCG
CTGGCGATGGTTACCGCCAACGTGGCCGATGCGCTGGGGATCGCGAAGGGCAGAATTTTACCCGCGGGGGATGCCGATCT
GTGCCTGTTCGATGATAATCTGCGCCCCGAACGCGTATACGCCCGCGGCCGCCTGATGCTGAAGGGAGAGGAGTGTCTGG
CCATGGGTAACTTTGAACAAGGAAACTGA

Upstream 100 bases:

>100_bases
CCCCGCCACGGGGAGCCGCCGCTCGTCATATCGACACAGCCACTCACCCGCCCCCGCGGCGGGTTGCGCTATCGTCATGC
CTAACACAGGAGAGAAGTCC

Downstream 100 bases:

>100_bases
GATAATGAGCCTACTGATCGCGCTGTGCGCCGTGCTGTTTGCCGGGCGCCTGATCCTGAAAAACTATAATCCCCAGGCAG
TATTGCTGCTGAGCGGCCTG

Product: isoaspartyl dipeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MFTLLKDADVYQPQPLGRRDVLLCGAKIVAIGENLQVSALPGLREVIDCRGQILAPGLIDQHVHLIGGGGEGGFASRTPA
VSLSRLIRAATTTVVGVLGTDGISRTPKDLYAKMQALNAEGISAYMHTGSYELPTRTITGTIRDDIAFIERVLGVKIALA
DHRGSFPTTQELLRIVSDIRVAALLAGKRGLLHIHLGALPDAFAQINALLAHGLPIQHISPTHVARTEALFADAIAFARA
GGHIDMTSGGSRFVAPACAARLALEAGVSPDRLTISSDGNGSVPRFDAQGSIIGLDAAPLDGNLQLLPQLIDQRIPLPQA
LAMVTANVADALGIAKGRILPAGDADLCLFDDNLRPERVYARGRLMLKGEECLAMGNFEQGN

Sequences:

>Translated_382_residues
MFTLLKDADVYQPQPLGRRDVLLCGAKIVAIGENLQVSALPGLREVIDCRGQILAPGLIDQHVHLIGGGGEGGFASRTPA
VSLSRLIRAATTTVVGVLGTDGISRTPKDLYAKMQALNAEGISAYMHTGSYELPTRTITGTIRDDIAFIERVLGVKIALA
DHRGSFPTTQELLRIVSDIRVAALLAGKRGLLHIHLGALPDAFAQINALLAHGLPIQHISPTHVARTEALFADAIAFARA
GGHIDMTSGGSRFVAPACAARLALEAGVSPDRLTISSDGNGSVPRFDAQGSIIGLDAAPLDGNLQLLPQLIDQRIPLPQA
LAMVTANVADALGIAKGRILPAGDADLCLFDDNLRPERVYARGRLMLKGEECLAMGNFEQGN
>Mature_382_residues
MFTLLKDADVYQPQPLGRRDVLLCGAKIVAIGENLQVSALPGLREVIDCRGQILAPGLIDQHVHLIGGGGEGGFASRTPA
VSLSRLIRAATTTVVGVLGTDGISRTPKDLYAKMQALNAEGISAYMHTGSYELPTRTITGTIRDDIAFIERVLGVKIALA
DHRGSFPTTQELLRIVSDIRVAALLAGKRGLLHIHLGALPDAFAQINALLAHGLPIQHISPTHVARTEALFADAIAFARA
GGHIDMTSGGSRFVAPACAARLALEAGVSPDRLTISSDGNGSVPRFDAQGSIIGLDAAPLDGNLQLLPQLIDQRIPLPQA
LAMVTANVADALGIAKGRILPAGDADLCLFDDNLRPERVYARGRLMLKGEECLAMGNFEQGN

Specific function: Catalyzes the hydrolytic cleavage of a subset of L- isoaspartyl (L-beta-aspartyl) dipeptides. Used to degrade proteins damaged by L-isoaspartyl residues formation. The best substrate for the enzyme reported thus far is iso-Asp-Leu [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M38 family [H]

Homologues:

Organism=Escherichia coli, GI1790784, Length=381, Percent_Identity=46.9816272965879, Blast_Score=317, Evalue=1e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR011059
- InterPro:   IPR010229 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.4.19.- [C]

Molecular weight: Translated: 40264; Mature: 40264

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00070 ALDEHYDE_DEHYDR_CYS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTLLKDADVYQPQPLGRRDVLLCGAKIVAIGENLQVSALPGLREVIDCRGQILAPGLID
CCEEECCCCCCCCCCCCCCCEEEECCEEEEECCCCEEEECCCHHHHHHCCCCEECCCHHC
QHVHLIGGGGEGGFASRTPAVSLSRLIRAATTTVVGVLGTDGISRTPKDLYAKMQALNAE
CCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHCCCC
GISAYMHTGSYELPTRTITGTIRDDIAFIERVLGVKIALADHRGSFPTTQELLRIVSDIR
CCEEEEECCCEECCCEEEECCHHHHHHHHHHHHCEEEEEECCCCCCCCHHHHHHHHHHHH
VAALLAGKRGLLHIHLGALPDAFAQINALLAHGLPIQHISPTHVARTEALFADAIAFARA
HHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
GGHIDMTSGGSRFVAPACAARLALEAGVSPDRLTISSDGNGSVPRFDAQGSIIGLDAAPL
CCEEEECCCCCEEEHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEECCCCCEEEEECCCC
DGNLQLLPQLIDQRIPLPQALAMVTANVADALGIAKGRILPAGDADLCLFDDNLRPERVY
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEECCCCCHHHEE
ARGRLMLKGEECLAMGNFEQGN
ECCEEEEECCCEEEECCCCCCC
>Mature Secondary Structure
MFTLLKDADVYQPQPLGRRDVLLCGAKIVAIGENLQVSALPGLREVIDCRGQILAPGLID
CCEEECCCCCCCCCCCCCCCEEEECCEEEEECCCCEEEECCCHHHHHHCCCCEECCCHHC
QHVHLIGGGGEGGFASRTPAVSLSRLIRAATTTVVGVLGTDGISRTPKDLYAKMQALNAE
CCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHCCCC
GISAYMHTGSYELPTRTITGTIRDDIAFIERVLGVKIALADHRGSFPTTQELLRIVSDIR
CCEEEEECCCEECCCEEEECCHHHHHHHHHHHHCEEEEEECCCCCCCCHHHHHHHHHHHH
VAALLAGKRGLLHIHLGALPDAFAQINALLAHGLPIQHISPTHVARTEALFADAIAFARA
HHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
GGHIDMTSGGSRFVAPACAARLALEAGVSPDRLTISSDGNGSVPRFDAQGSIIGLDAAPL
CCEEEECCCCCEEEHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEECCCCCEEEEECCCC
DGNLQLLPQLIDQRIPLPQALAMVTANVADALGIAKGRILPAGDADLCLFDDNLRPERVY
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEECCCCCHHHEE
ARGRLMLKGEECLAMGNFEQGN
ECCEEEEECCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7876157; 7610040; 9278503 [H]