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The map label for this gene is dosC [H]

Identifier: 238917783

GI number: 238917783

Start: 1925535

End: 1927331

Strand: Reverse

Name: dosC [H]

Synonym: EUBELI_01864

Alternate gene names: 238917783

Gene position: 1927331-1925535 (Counterclockwise)

Preceding gene: 238917784

Following gene: 238917782

Centisome position: 89.89

GC content: 33.95

Gene sequence:

>1797_bases
ATGGTGGATTTTTTTAAAGCTGAGACTGTTAATTCAGACATTCTTATTGATGAGACAATGTCAATTGTAACAGCAGATGA
AAATTTTGAAGCAATTTCAGGCAATAAAGCACTTTTTATATATACAAGATATATACACCCTGTCGATGTCAGCAGATTCA
CAGAAGCATTAAAGGATTACGCTGAATCCGGTAAGTATATTGTTGTAAGAATGTTATATCAGACAGGCGAATATCACTGG
ATGCTTACAAGAATTACAGATGGCGGTGTATCTGAGACAAGAGGTCATATGTATGAAATTAATATTATTGATGCCGCATT
AATTACTACTCAGATAGATAACCTTAATTCGCAGATTAAGAGATATTCTAGCTATCTTGGCATGTGTGAGAATGTCCTGT
TTTCTTATGATATTCTTAATGATGACTTTAATATATTTATGACATCAGATGGACACCAGACTTTGTCTTTTTACCGTGGA
ACATTAAATGCATGGGAAGAAGATAAGATAAAGACTGATGCACTTGGGCTGAATGAGGTAAAAGAACTTGATGGATTTTG
CAAGGCACTTGCTAATGGTGAGAAGTTATTCAAGCGGGAGATTACTATTAATACTCTTAATAAGCTTGGAAGACTGGATA
AATGTCTTGTGAGAGGTAATACGATTGTTGATGAATATGGAAACAGTATCGTTATAGGTACTATTATAATACTTGAGAGT
TCAGATAATCATGTAGTTGAAGAACTCAATATAATATCTGATATGAAAGATCCTGGTACAGATTTGCTTAATAAGCGCGC
TATTACTGATTATGTAAGAAAGCTGATAGATTCACAGCCGGGACATACTGTAACAATTGCTATTATTGATGTTGATGATT
TTAAGACTATTAATGATACATATGGACATATGTTTGGTGACGAAGTTCTTTGTAAGGTTGCAGATATCTTAAGAGATGCA
GTTGGAAGCAGAGGATTGTGTGGAAGAATTGGCGGAGATGAGATGTTTATCATTATGGAAGGACTTAATGATAACGAGGG
TATAAGGAATGTTTTAAGAACAATCCGCAATAATACAAAATGGCTGTATCATGATGATCCGCGTAATATTAAGATTACAT
GTTCAATTGGTTCAGCAACTTATCCTAATGATGCCAAGAGTTATGATGAATTATTTAAGATAGCTGATAAGGTTCTTTAC
CTTGCTAAAGAAAAGGGAAAAGACAGATATATCATTTACCATGAGGATATACATAGGGAATATGTATATGGAATGGGAAG
GATTGTTGACCTTAATGATAAAGTGTTTTACAAGTACCATAAGATGGAGGTTGTTAATACCATTATCAGGGAATACAAGG
AAGCTGATGACGCAAGAAGAAAGGAACTGATAGATATTGTGGCAGTAGCATTTAATATTAATACTATTGCAATATATGAC
AGAACTGAGCTGACAAAGCATATTCTGTATGGTGACCAGCGTATGACAGATGATGACGGAAGCTTTTTCAAGGAAGATAA
TTATATTCCCAACTTTAGGGAAGATGGCATATTTGTAATAGATAACATCAACTTTTTTGAAACAAAGGCTCCAGCAGTAT
ATAAAGTGTATTCTGAATACGGAATTGTTCAGGCTGTCCAGTACATTATAGGTGGAGATATTAAGAAGAATAACAATATT
ATTTCTTATGGAAGATATAAGCTTGATAAGAAATGGGCAGAGAGCGACATGAATTTCCTTGCTATTATAGGTGATTACAT
AGGAAGAATATTCCTGAAAGAGAGAAAACATGATTAA

Upstream 100 bases:

>100_bases
TACAGACCGGATTTGTATGAAGAATATGTTAAGAAACATTCTGATGAATTCAGATGATATAGGGAGAGGCACAATTAATT
ATAAAATAGGGGTTTAAGCT

Downstream 100 bases:

>100_bases
CAGATATGTTGCACTGGATATTGAGACTACAGGTCTTAATCCGGCTGTTGACAGAATTATTGAAGTTGGAATGGCAAGAG
TGGAAGATGGTCAGATAACA

Product: two-component system PleD-like family response regulator

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MVDFFKAETVNSDILIDETMSIVTADENFEAISGNKALFIYTRYIHPVDVSRFTEALKDYAESGKYIVVRMLYQTGEYHW
MLTRITDGGVSETRGHMYEINIIDAALITTQIDNLNSQIKRYSSYLGMCENVLFSYDILNDDFNIFMTSDGHQTLSFYRG
TLNAWEEDKIKTDALGLNEVKELDGFCKALANGEKLFKREITINTLNKLGRLDKCLVRGNTIVDEYGNSIVIGTIIILES
SDNHVVEELNIISDMKDPGTDLLNKRAITDYVRKLIDSQPGHTVTIAIIDVDDFKTINDTYGHMFGDEVLCKVADILRDA
VGSRGLCGRIGGDEMFIIMEGLNDNEGIRNVLRTIRNNTKWLYHDDPRNIKITCSIGSATYPNDAKSYDELFKIADKVLY
LAKEKGKDRYIIYHEDIHREYVYGMGRIVDLNDKVFYKYHKMEVVNTIIREYKEADDARRKELIDIVAVAFNINTIAIYD
RTELTKHILYGDQRMTDDDGSFFKEDNYIPNFREDGIFVIDNINFFETKAPAVYKVYSEYGIVQAVQYIIGGDIKKNNNI
ISYGRYKLDKKWAESDMNFLAIIGDYIGRIFLKERKHD

Sequences:

>Translated_598_residues
MVDFFKAETVNSDILIDETMSIVTADENFEAISGNKALFIYTRYIHPVDVSRFTEALKDYAESGKYIVVRMLYQTGEYHW
MLTRITDGGVSETRGHMYEINIIDAALITTQIDNLNSQIKRYSSYLGMCENVLFSYDILNDDFNIFMTSDGHQTLSFYRG
TLNAWEEDKIKTDALGLNEVKELDGFCKALANGEKLFKREITINTLNKLGRLDKCLVRGNTIVDEYGNSIVIGTIIILES
SDNHVVEELNIISDMKDPGTDLLNKRAITDYVRKLIDSQPGHTVTIAIIDVDDFKTINDTYGHMFGDEVLCKVADILRDA
VGSRGLCGRIGGDEMFIIMEGLNDNEGIRNVLRTIRNNTKWLYHDDPRNIKITCSIGSATYPNDAKSYDELFKIADKVLY
LAKEKGKDRYIIYHEDIHREYVYGMGRIVDLNDKVFYKYHKMEVVNTIIREYKEADDARRKELIDIVAVAFNINTIAIYD
RTELTKHILYGDQRMTDDDGSFFKEDNYIPNFREDGIFVIDNINFFETKAPAVYKVYSEYGIVQAVQYIIGGDIKKNNNI
ISYGRYKLDKKWAESDMNFLAIIGDYIGRIFLKERKHD
>Mature_598_residues
MVDFFKAETVNSDILIDETMSIVTADENFEAISGNKALFIYTRYIHPVDVSRFTEALKDYAESGKYIVVRMLYQTGEYHW
MLTRITDGGVSETRGHMYEINIIDAALITTQIDNLNSQIKRYSSYLGMCENVLFSYDILNDDFNIFMTSDGHQTLSFYRG
TLNAWEEDKIKTDALGLNEVKELDGFCKALANGEKLFKREITINTLNKLGRLDKCLVRGNTIVDEYGNSIVIGTIIILES
SDNHVVEELNIISDMKDPGTDLLNKRAITDYVRKLIDSQPGHTVTIAIIDVDDFKTINDTYGHMFGDEVLCKVADILRDA
VGSRGLCGRIGGDEMFIIMEGLNDNEGIRNVLRTIRNNTKWLYHDDPRNIKITCSIGSATYPNDAKSYDELFKIADKVLY
LAKEKGKDRYIIYHEDIHREYVYGMGRIVDLNDKVFYKYHKMEVVNTIIREYKEADDARRKELIDIVAVAFNINTIAIYD
RTELTKHILYGDQRMTDDDGSFFKEDNYIPNFREDGIFVIDNINFFETKAPAVYKVYSEYGIVQAVQYIIGGDIKKNNNI
ISYGRYKLDKKWAESDMNFLAIIGDYIGRIFLKERKHD

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=161, Percent_Identity=37.2670807453416, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787262, Length=174, Percent_Identity=28.735632183908, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI87082007, Length=157, Percent_Identity=30.5732484076433, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1787541, Length=180, Percent_Identity=25.5555555555556, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786584, Length=165, Percent_Identity=32.1212121212121, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI87081881, Length=198, Percent_Identity=32.3232323232323, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1787056, Length=135, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788085, Length=178, Percent_Identity=25.8426966292135, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1787802, Length=130, Percent_Identity=30.7692307692308, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1788956, Length=162, Percent_Identity=29.0123456790123, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1787816, Length=170, Percent_Identity=30, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI87081977, Length=171, Percent_Identity=28.6549707602339, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 68775; Mature: 68775

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDFFKAETVNSDILIDETMSIVTADENFEAISGNKALFIYTRYIHPVDVSRFTEALKDY
CCCCEECCCCCCCEEEECCEEEEEECCCCEEECCCCEEEEEEEEECCCCHHHHHHHHHHH
AESGKYIVVRMLYQTGEYHWMLTRITDGGVSETRGHMYEINIIDAALITTQIDNLNSQIK
HHCCCEEEEEEEECCCCEEEEEEEEECCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHH
RYSSYLGMCENVLFSYDILNDDFNIFMTSDGHQTLSFYRGTLNAWEEDKIKTDALGLNEV
HHHHHHHHHHHHHEEEEEECCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHH
KELDGFCKALANGEKLFKREITINTLNKLGRLDKCLVRGNTIVDEYGNSIVIGTIIILES
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHCCCEEEEEEEEEEEC
SDNHVVEELNIISDMKDPGTDLLNKRAITDYVRKLIDSQPGHTVTIAIIDVDDFKTINDT
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHH
YGHMFGDEVLCKVADILRDAVGSRGLCGRIGGDEMFIIMEGLNDNEGIRNVLRTIRNNTK
HHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEECCCCCHHHHHHHHHHHCCCC
WLYHDDPRNIKITCSIGSATYPNDAKSYDELFKIADKVLYLAKEKGKDRYIIYHEDIHRE
EEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHH
YVYGMGRIVDLNDKVFYKYHKMEVVNTIIREYKEADDARRKELIDIVAVAFNINTIAIYD
HHHCCCEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECEEEEEEC
RTELTKHILYGDQRMTDDDGSFFKEDNYIPNFREDGIFVIDNINFFETKAPAVYKVYSEY
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEECCCCCHHHHHHHHH
GIVQAVQYIIGGDIKKNNNIISYGRYKLDKKWAESDMNFLAIIGDYIGRIFLKERKHD
HHHHHHHHHHCCCEECCCCEEEECCEEHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVDFFKAETVNSDILIDETMSIVTADENFEAISGNKALFIYTRYIHPVDVSRFTEALKDY
CCCCEECCCCCCCEEEECCEEEEEECCCCEEECCCCEEEEEEEEECCCCHHHHHHHHHHH
AESGKYIVVRMLYQTGEYHWMLTRITDGGVSETRGHMYEINIIDAALITTQIDNLNSQIK
HHCCCEEEEEEEECCCCEEEEEEEEECCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHH
RYSSYLGMCENVLFSYDILNDDFNIFMTSDGHQTLSFYRGTLNAWEEDKIKTDALGLNEV
HHHHHHHHHHHHHEEEEEECCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHH
KELDGFCKALANGEKLFKREITINTLNKLGRLDKCLVRGNTIVDEYGNSIVIGTIIILES
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHCCCEEEEEEEEEEEC
SDNHVVEELNIISDMKDPGTDLLNKRAITDYVRKLIDSQPGHTVTIAIIDVDDFKTINDT
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHH
YGHMFGDEVLCKVADILRDAVGSRGLCGRIGGDEMFIIMEGLNDNEGIRNVLRTIRNNTK
HHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEECCCCCHHHHHHHHHHHCCCC
WLYHDDPRNIKITCSIGSATYPNDAKSYDELFKIADKVLYLAKEKGKDRYIIYHEDIHRE
EEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHH
YVYGMGRIVDLNDKVFYKYHKMEVVNTIIREYKEADDARRKELIDIVAVAFNINTIAIYD
HHHCCCEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECEEEEEEC
RTELTKHILYGDQRMTDDDGSFFKEDNYIPNFREDGIFVIDNINFFETKAPAVYKVYSEY
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEECCCCCHHHHHHHHH
GIVQAVQYIIGGDIKKNNNIISYGRYKLDKKWAESDMNFLAIIGDYIGRIFLKERKHD
HHHHHHHHHHCCCEECCCCEEEECCEEHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]