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The map label for this gene is trmD

Identifier: 238917784

GI number: 238917784

Start: 1927375

End: 1928085

Strand: Reverse

Name: trmD

Synonym: EUBELI_01865

Alternate gene names: 238917784

Gene position: 1928085-1927375 (Counterclockwise)

Preceding gene: 238917785

Following gene: 238917783

Centisome position: 89.92

GC content: 38.96

Gene sequence:

>711_bases
ATGGATTTTCATGTTATGACATTGTTCCCTGATATGATAATGGATGGGCTTAATACAAGTATTACAGGCAGAGCCATAAA
GTCGGGGGTTATGTCTGTAACAGCACATGACATAAGGGATTATTCTAATGATAAGCATTTAAAGGTTGACGATTATCCTT
ATGGTGGTGGTGCAGGAATGGTTATGAGAGCTGCTCCTGTCTGCGACTGTTATGAGGATATCGTCAAGAATATAGGAAAA
AGACCGCGTGTTATATATATGACACCTCAGGGGTATACATTTACACAGAAGATGGCAGAAGATTTTGCAAAGGAAGATAA
TCTCGTTATTTTGTGTGGACATTATGAAGGTATTGATGAGAGGGCATTGGAGAATATAGTAACAGATTATGTATCAATCG
GTGACTATGTTCTTACAGGTGGAGAACTGCCGGCAATGGTAGTTATAGATACTATTTCAAGACTGGTTCCGGGAGTTCTT
AATAATGAGGAATCAGCTGAAACGGAGAGCTTTTCCGATGGGCTGCTTGAGTATCCGCAATACACAAGACCAGCAGACTA
TAATGGACAATTGGTTCCCGAAGTACTTTTGTCAGGACATCATGCTAATATTGAAAAATGGAGACATGAGAAGTCGATTG
AAAGAACGAAAAAGTACAGACCGGATTTGTATGAAGAATATGTTAAGAAACATTCTGATGAATTCAGATGA

Upstream 100 bases:

>100_bases
GAACAGGTTATCTGGGTTCATCTTATACTGATAGCGGATATAATCTTAAGAGTGGATATTATGCATTCTTGCTTAATGCA
ATTGCAGGAGTAGTTAAATA

Downstream 100 bases:

>100_bases
TATAGGGAGAGGCACAATTAATTATAAAATAGGGGTTTAAGCTATGGTGGATTTTTTTAAAGCTGAGACTGTTAATTCAG
ACATTCTTATTGATGAGACA

Product: tRNA (guanine-N1-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MDFHVMTLFPDMIMDGLNTSITGRAIKSGVMSVTAHDIRDYSNDKHLKVDDYPYGGGAGMVMRAAPVCDCYEDIVKNIGK
RPRVIYMTPQGYTFTQKMAEDFAKEDNLVILCGHYEGIDERALENIVTDYVSIGDYVLTGGELPAMVVIDTISRLVPGVL
NNEESAETESFSDGLLEYPQYTRPADYNGQLVPEVLLSGHHANIEKWRHEKSIERTKKYRPDLYEEYVKKHSDEFR

Sequences:

>Translated_236_residues
MDFHVMTLFPDMIMDGLNTSITGRAIKSGVMSVTAHDIRDYSNDKHLKVDDYPYGGGAGMVMRAAPVCDCYEDIVKNIGK
RPRVIYMTPQGYTFTQKMAEDFAKEDNLVILCGHYEGIDERALENIVTDYVSIGDYVLTGGELPAMVVIDTISRLVPGVL
NNEESAETESFSDGLLEYPQYTRPADYNGQLVPEVLLSGHHANIEKWRHEKSIERTKKYRPDLYEEYVKKHSDEFR
>Mature_236_residues
MDFHVMTLFPDMIMDGLNTSITGRAIKSGVMSVTAHDIRDYSNDKHLKVDDYPYGGGAGMVMRAAPVCDCYEDIVKNIGK
RPRVIYMTPQGYTFTQKMAEDFAKEDNLVILCGHYEGIDERALENIVTDYVSIGDYVLTGGELPAMVVIDTISRLVPGVL
NNEESAETESFSDGLLEYPQYTRPADYNGQLVPEVLLSGHHANIEKWRHEKSIERTKKYRPDLYEEYVKKHSDEFR

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=225, Percent_Identity=45.7777777777778, Blast_Score=217, Evalue=4e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_EUBE2 (C4Z429)

Other databases:

- EMBL:   CP001104
- RefSeq:   YP_002931301.1
- GeneID:   7957922
- GenomeReviews:   CP001104_GR
- KEGG:   eel:EUBELI_01865
- OMA:   NRYDLYL
- ProtClustDB:   CLSK2540957
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 26788; Mature: 26788

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFHVMTLFPDMIMDGLNTSITGRAIKSGVMSVTAHDIRDYSNDKHLKVDDYPYGGGAGM
CCEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCH
VMRAAPVCDCYEDIVKNIGKRPRVIYMTPQGYTFTQKMAEDFAKEDNLVILCGHYEGIDE
HHHHCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHH
RALENIVTDYVSIGDYVLTGGELPAMVVIDTISRLVPGVLNNEESAETESFSDGLLEYPQ
HHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHCCCC
YTRPADYNGQLVPEVLLSGHHANIEKWRHEKSIERTKKYRPDLYEEYVKKHSDEFR
CCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDFHVMTLFPDMIMDGLNTSITGRAIKSGVMSVTAHDIRDYSNDKHLKVDDYPYGGGAGM
CCEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCH
VMRAAPVCDCYEDIVKNIGKRPRVIYMTPQGYTFTQKMAEDFAKEDNLVILCGHYEGIDE
HHHHCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHH
RALENIVTDYVSIGDYVLTGGELPAMVVIDTISRLVPGVLNNEESAETESFSDGLLEYPQ
HHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHCCCC
YTRPADYNGQLVPEVLLSGHHANIEKWRHEKSIERTKKYRPDLYEEYVKKHSDEFR
CCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA