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The map label for this gene is recJ [H]

Identifier: 238917314

GI number: 238917314

Start: 1431460

End: 1433175

Strand: Reverse

Name: recJ [H]

Synonym: EUBELI_01389

Alternate gene names: 238917314

Gene position: 1433175-1431460 (Counterclockwise)

Preceding gene: 238917315

Following gene: 238917309

Centisome position: 66.84

GC content: 38.29

Gene sequence:

>1716_bases
GTGAAAAGCAACTGGAAAGTATACACTAAGAAAGCTGATTTCAAAGCAATTGGGGAAAGATTTGGCATTGACCAGGTTAT
GGCAAGAATTATCCGTAACAGAGGGTTAGAAACGGACGAGCAGATAAATATGTATCTTAATGGAACCGTGGATGATATGC
ATTCACCACATTTATTAAAGGATGCAGACAAATGTGTTGATATCCTGACAGACAAGATACATGCTGGGAAGAAAATACAT
ATTATCGGTGATTATGATATAGATGGGATATGTTCAACAACTATTCTTTATAAAGGGCTAAAGGCAGCGGGAGCAGATGT
TACATTTGCAGTACCAGACAGAATTATTGACGGATACGGTATTAATGAGCATCTGATAGATGAAGCATATAATAATGGAA
CTGATACGATTATTACATGTGATAATGGAATTGCTGCGATTGAGCAGATTAAATATGCTAAAGAAAAGGGAATGACAGTA
ATTGTTACTGACCATCATGATATTCCGTTTGACTTTGTTGATGGAGAAAAGATACATAAAGTACCACAGGCAGATGCAAT
TGTTAATCCTAAGCAGGAGGACTGTCCGTATCCATTTGATAAGCTGTGTGGAGCCGGAGTAGCCTTCAAGGTTATTAAGA
TTCTATATGAGAGACTTGGACTTAATCCAATCGATCTTGAAGAATATGCAGAGCTTATGGCAATTGCAACAATAGGAGAT
GTAGTTGATTTATCAGATGAGAACAGGGTAATTGTCAAATATGGTTTAAAGCATCTGGCAGTAACTTCTAATATCGGTAT
AAGAGCGCTTGTAGAGGCGTGTGAGCTTGAAATAGACAAGATAAGCTCATATCACATAGGATTTGTTATAGGTCCATGTC
TTAATGCGACAGGAAGACTTGATTCAGCAAGAAGAGCCATTGAGCTGCTTCTTACTACAGATATGGAAGATGCGAGAAAG
AAGGCTTTAGAGCTTCGCACACTTAATGTTGAAAGAAAAGATATAACAGAAGAATATGCCGCTATTGCAATTGAACAGGT
TGAGAACACTGAACTAAAAGACGATAAGGTGTTAGTTGTGTATCTTCCTGAATGCCATGAAAGTGTTGCAGGGATAATTG
CAGGAAGGGTAAGAGAAAAATTCTACAGACCTTCAATAGTAATCACTAAGGCAGAAGATGGTGCAAAGGGCTCAGGGCGT
TCAATAGAAGGCTATAACATGTTTGAGGAGATAAGCAAATGTGGTAAACTCCTTAACAAATATGGCGGACATCCAATGGC
TGCCGGAATATCGCTTGATATTGATAAAATTGATGAATTCAGGAAAGCGCTGAATGAGAATCAGACAATGACAGAAAATG
TACTAACGCCGACAGTGTGGATTGATGTGCCTATGCCGGTTGATTATGTTGATATAAAACTTATAAAACAGTTTGAGAAG
CTGCAGCCTTTCGGGAAAGGTAATGAAAAACCGGTGTTTGCAGACAGAAATCTTACTGTCAGACAGTCAGCTGTGATTGG
AAAAAATAAAAATGTATTAAGATGTCAGTTAGAAAGTGAGCATGGCAAGCTGGTGACAGCAATCAGGTTCAAATTAGACG
GACAGGAGATACCAGAGGTTGGAAGGAAGATATCTATGGTGTATTACCCGGATATTAATGAATATAATGGGATTGTTTCA
ATACAGTTTCGGATAGAAGACTGGAGATATGTGTAA

Upstream 100 bases:

>100_bases
TCTCAGGTTTAATTAACAGATACAATAAGTTTATGACATGGCGGGGATTGTATATCAGTTCCCGCCATAATTGGTTTAAT
AATGAAACAGGGAGAAAAGA

Downstream 100 bases:

>100_bases
ATAGCAGCCTTCCAATAAAAGAACCGTCGGTTCTTTGACCTGTATAGGTCAATGGGACCGGCGGTTTATATATTCTGTCT
TATACTTAGAATAGACGATT

Product: single-stranded-DNA-specific exonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MKSNWKVYTKKADFKAIGERFGIDQVMARIIRNRGLETDEQINMYLNGTVDDMHSPHLLKDADKCVDILTDKIHAGKKIH
IIGDYDIDGICSTTILYKGLKAAGADVTFAVPDRIIDGYGINEHLIDEAYNNGTDTIITCDNGIAAIEQIKYAKEKGMTV
IVTDHHDIPFDFVDGEKIHKVPQADAIVNPKQEDCPYPFDKLCGAGVAFKVIKILYERLGLNPIDLEEYAELMAIATIGD
VVDLSDENRVIVKYGLKHLAVTSNIGIRALVEACELEIDKISSYHIGFVIGPCLNATGRLDSARRAIELLLTTDMEDARK
KALELRTLNVERKDITEEYAAIAIEQVENTELKDDKVLVVYLPECHESVAGIIAGRVREKFYRPSIVITKAEDGAKGSGR
SIEGYNMFEEISKCGKLLNKYGGHPMAAGISLDIDKIDEFRKALNENQTMTENVLTPTVWIDVPMPVDYVDIKLIKQFEK
LQPFGKGNEKPVFADRNLTVRQSAVIGKNKNVLRCQLESEHGKLVTAIRFKLDGQEIPEVGRKISMVYYPDINEYNGIVS
IQFRIEDWRYV

Sequences:

>Translated_571_residues
MKSNWKVYTKKADFKAIGERFGIDQVMARIIRNRGLETDEQINMYLNGTVDDMHSPHLLKDADKCVDILTDKIHAGKKIH
IIGDYDIDGICSTTILYKGLKAAGADVTFAVPDRIIDGYGINEHLIDEAYNNGTDTIITCDNGIAAIEQIKYAKEKGMTV
IVTDHHDIPFDFVDGEKIHKVPQADAIVNPKQEDCPYPFDKLCGAGVAFKVIKILYERLGLNPIDLEEYAELMAIATIGD
VVDLSDENRVIVKYGLKHLAVTSNIGIRALVEACELEIDKISSYHIGFVIGPCLNATGRLDSARRAIELLLTTDMEDARK
KALELRTLNVERKDITEEYAAIAIEQVENTELKDDKVLVVYLPECHESVAGIIAGRVREKFYRPSIVITKAEDGAKGSGR
SIEGYNMFEEISKCGKLLNKYGGHPMAAGISLDIDKIDEFRKALNENQTMTENVLTPTVWIDVPMPVDYVDIKLIKQFEK
LQPFGKGNEKPVFADRNLTVRQSAVIGKNKNVLRCQLESEHGKLVTAIRFKLDGQEIPEVGRKISMVYYPDINEYNGIVS
IQFRIEDWRYV
>Mature_571_residues
MKSNWKVYTKKADFKAIGERFGIDQVMARIIRNRGLETDEQINMYLNGTVDDMHSPHLLKDADKCVDILTDKIHAGKKIH
IIGDYDIDGICSTTILYKGLKAAGADVTFAVPDRIIDGYGINEHLIDEAYNNGTDTIITCDNGIAAIEQIKYAKEKGMTV
IVTDHHDIPFDFVDGEKIHKVPQADAIVNPKQEDCPYPFDKLCGAGVAFKVIKILYERLGLNPIDLEEYAELMAIATIGD
VVDLSDENRVIVKYGLKHLAVTSNIGIRALVEACELEIDKISSYHIGFVIGPCLNATGRLDSARRAIELLLTTDMEDARK
KALELRTLNVERKDITEEYAAIAIEQVENTELKDDKVLVVYLPECHESVAGIIAGRVREKFYRPSIVITKAEDGAKGSGR
SIEGYNMFEEISKCGKLLNKYGGHPMAAGISLDIDKIDEFRKALNENQTMTENVLTPTVWIDVPMPVDYVDIKLIKQFEK
LQPFGKGNEKPVFADRNLTVRQSAVIGKNKNVLRCQLESEHGKLVTAIRFKLDGQEIPEVGRKISMVYYPDINEYNGIVS
IQFRIEDWRYV

Specific function: Putative single-stranded-DNA-specific exonuclease. RecA thread formation during DNA double-strand break repair requires RecJ or AadAB [H]

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OB DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=565, Percent_Identity=32.212389380531, Blast_Score=288, Evalue=5e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610
- InterPro:   IPR018779 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1; PF10141 ssDNA-exonuc_C [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 64046; Mature: 64046

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSNWKVYTKKADFKAIGERFGIDQVMARIIRNRGLETDEQINMYLNGTVDDMHSPHLLK
CCCCCEEEEECCCHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEEECCCHHCCCCCCHHH
DADKCVDILTDKIHAGKKIHIIGDYDIDGICSTTILYKGLKAAGADVTFAVPDRIIDGYG
HHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHCCCC
INEHLIDEAYNNGTDTIITCDNGIAAIEQIKYAKEKGMTVIVTDHHDIPFDFVDGEKIHK
CCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHC
VPQADAIVNPKQEDCPYPFDKLCGAGVAFKVIKILYERLGLNPIDLEEYAELMAIATIGD
CCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
VVDLSDENRVIVKYGLKHLAVTSNIGIRALVEACELEIDKISSYHIGFVIGPCLNATGRL
EEECCCCCEEEEEECCHHEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCC
DSARRAIELLLTTDMEDARKKALELRTLNVERKDITEEYAAIAIEQVENTELKDDKVLVV
HHHHHHHHHHHCCCHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
YLPECHESVAGIIAGRVREKFYRPSIVITKAEDGAKGSGRSIEGYNMFEEISKCGKLLNK
ECCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
YGGHPMAAGISLDIDKIDEFRKALNENQTMTENVLTPTVWIDVPMPVDYVDIKLIKQFEK
HCCCCEECCEEEEHHHHHHHHHHHCCCCCCHHHCCCCEEEEECCCCCCHHHHHHHHHHHH
LQPFGKGNEKPVFADRNLTVRQSAVIGKNKNVLRCQLESEHGKLVTAIRFKLDGQEIPEV
HCCCCCCCCCCEEECCCCEEEHHEEECCCCCEEEEEEECCCCCEEEEEEEEECCHHHHHH
GRKISMVYYPDINEYNGIVSIQFRIEDWRYV
CCEEEEEECCCCCCCCCEEEEEEEECCCCCC
>Mature Secondary Structure
MKSNWKVYTKKADFKAIGERFGIDQVMARIIRNRGLETDEQINMYLNGTVDDMHSPHLLK
CCCCCEEEEECCCHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEEECCCHHCCCCCCHHH
DADKCVDILTDKIHAGKKIHIIGDYDIDGICSTTILYKGLKAAGADVTFAVPDRIIDGYG
HHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHCCCC
INEHLIDEAYNNGTDTIITCDNGIAAIEQIKYAKEKGMTVIVTDHHDIPFDFVDGEKIHK
CCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHC
VPQADAIVNPKQEDCPYPFDKLCGAGVAFKVIKILYERLGLNPIDLEEYAELMAIATIGD
CCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
VVDLSDENRVIVKYGLKHLAVTSNIGIRALVEACELEIDKISSYHIGFVIGPCLNATGRL
EEECCCCCEEEEEECCHHEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCC
DSARRAIELLLTTDMEDARKKALELRTLNVERKDITEEYAAIAIEQVENTELKDDKVLVV
HHHHHHHHHHHCCCHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
YLPECHESVAGIIAGRVREKFYRPSIVITKAEDGAKGSGRSIEGYNMFEEISKCGKLLNK
ECCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
YGGHPMAAGISLDIDKIDEFRKALNENQTMTENVLTPTVWIDVPMPVDYVDIKLIKQFEK
HCCCCEECCEEEEHHHHHHHHHHHCCCCCCHHHCCCCEEEEECCCCCCHHHHHHHHHHHH
LQPFGKGNEKPVFADRNLTVRQSAVIGKNKNVLRCQLESEHGKLVTAIRFKLDGQEIPEV
HCCCCCCCCCCEEECCCCEEEHHEEECCCCCEEEEEEECCCCCEEEEEEEEECCHHHHHH
GRKISMVYYPDINEYNGIVSIQFRIEDWRYV
CCEEEEEECCCCCCCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]