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The map label for this gene is leuD

Identifier: 238917292

GI number: 238917292

Start: 1407305

End: 1407799

Strand: Reverse

Name: leuD

Synonym: EUBELI_01367

Alternate gene names: 238917292

Gene position: 1407799-1407305 (Counterclockwise)

Preceding gene: 238917293

Following gene: 238917291

Centisome position: 65.66

GC content: 37.98

Gene sequence:

>495_bases
ATGAACGAGAAATTTAGCGGTAAAGTATGGGTACTTGGTGATGACATTGATACAGATATCATTATCCCTACAGAGTACCT
TGCATTAAAGACAGTAGAAGATATGAAACCATATGCATTTTCGCCATTAAGACCAGAACTTGCAGGTCAGATTAAGCCAG
GCGACATCATTGTTGCAGGAAAGAACTTTGGTTGTGGTTCATCAAGAGAGCAGGCACCTGAGGTTATTAAGGCATTAGGT
ATTAAATGTGTTATTGCCAAATCATATGCAAGAATATTCTTCAGAAATTCTATTAATAACGGACTTCTCCTTATAGAGAA
TGCAGACCTTCACGACGAAGTTACGGAAGGCGATACTATTGATGTTGAAGTAAATGCAAAGATTACACACAATGGAAAAG
CATATCCTATTGCTTCACTTCCTGATAATCTTGTGGATATACTTAATGCAGGCGGACTTGTTAAGGCAATGAGAAAGCTT
AACGGTCTTGACTAA

Upstream 100 bases:

>100_bases
ATCTTGCTTCAGCGGCTACTGTTACAGCATCAGCAATCAAGGGCGAGATAGCAACAGCAGACATGGTATAAGACAAGGAT
TTAACTTCAAAGGAGTAATT

Downstream 100 bases:

>100_bases
GACAGGGAGAGGTAAGAATAATGGGACATACAATTATTGAGAAGATTATTGGAAAGAATATTGGCAGGGAAGTAAAGCCG
GGAGATATCGTAACTGTTAA

Product: 3-isopropylmalate/(R)-2-methylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MNEKFSGKVWVLGDDIDTDIIIPTEYLALKTVEDMKPYAFSPLRPELAGQIKPGDIIVAGKNFGCGSSREQAPEVIKALG
IKCVIAKSYARIFFRNSINNGLLLIENADLHDEVTEGDTIDVEVNAKITHNGKAYPIASLPDNLVDILNAGGLVKAMRKL
NGLD

Sequences:

>Translated_164_residues
MNEKFSGKVWVLGDDIDTDIIIPTEYLALKTVEDMKPYAFSPLRPELAGQIKPGDIIVAGKNFGCGSSREQAPEVIKALG
IKCVIAKSYARIFFRNSINNGLLLIENADLHDEVTEGDTIDVEVNAKITHNGKAYPIASLPDNLVDILNAGGLVKAMRKL
NGLD
>Mature_164_residues
MNEKFSGKVWVLGDDIDTDIIIPTEYLALKTVEDMKPYAFSPLRPELAGQIKPGDIIVAGKNFGCGSSREQAPEVIKALG
IKCVIAKSYARIFFRNSINNGLLLIENADLHDEVTEGDTIDVEVNAKITHNGKAYPIASLPDNLVDILNAGGLVKAMRKL
NGLD

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=108, Percent_Identity=37.962962962963, Blast_Score=71, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320440, Length=107, Percent_Identity=42.9906542056075, Blast_Score=79, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6321429, Length=106, Percent_Identity=39.622641509434, Blast_Score=71, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_EUBE2 (C4Z1K1)

Other databases:

- EMBL:   CP001104
- RefSeq:   YP_002930809.1
- GeneID:   7958222
- GenomeReviews:   CP001104_GR
- KEGG:   eel:EUBELI_01367
- OMA:   VAGKNMG
- HAMAP:   MF_01032
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR02087

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 17825; Mature: 17825

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEKFSGKVWVLGDDIDTDIIIPTEYLALKTVEDMKPYAFSPLRPELAGQIKPGDIIVAG
CCCCCCCEEEEECCCCCCCEEECCHHHEEEEHHHCCCCCCCCCCHHHCCCCCCCCEEEEC
KNFGCGSSREQAPEVIKALGIKCVIAKSYARIFFRNSINNGLLLIENADLHDEVTEGDTI
CCCCCCCCHHHHHHHHHHHCCEEEEEHHHHHHHEECCCCCCEEEEECCCCCCCCCCCCEE
DVEVNAKITHNGKAYPIASLPDNLVDILNAGGLVKAMRKLNGLD
EEEEEEEEEECCCEEEECCCCHHHHHHHCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MNEKFSGKVWVLGDDIDTDIIIPTEYLALKTVEDMKPYAFSPLRPELAGQIKPGDIIVAG
CCCCCCCEEEEECCCCCCCEEECCHHHEEEEHHHCCCCCCCCCCHHHCCCCCCCCEEEEC
KNFGCGSSREQAPEVIKALGIKCVIAKSYARIFFRNSINNGLLLIENADLHDEVTEGDTI
CCCCCCCCHHHHHHHHHHHCCEEEEEHHHHHHHEECCCCCCEEEEECCCCCCCCCCCCEE
DVEVNAKITHNGKAYPIASLPDNLVDILNAGGLVKAMRKLNGLD
EEEEEEEEEECCCEEEECCCCHHHHHHHCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA