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The map label for this gene is leuC1 [H]

Identifier: 238917291

GI number: 238917291

Start: 1406043

End: 1407284

Strand: Reverse

Name: leuC1 [H]

Synonym: EUBELI_01366

Alternate gene names: 238917291

Gene position: 1407284-1406043 (Counterclockwise)

Preceding gene: 238917292

Following gene: 238917289

Centisome position: 65.63

GC content: 41.22

Gene sequence:

>1242_bases
ATGGGACATACAATTATTGAGAAGATTATTGGAAAGAATATTGGCAGGGAAGTAAAGCCGGGAGATATCGTAACTGTTAA
TGTTGACAGAGTAATGATTCATGATATTTTCATTCCATTTGTTAAAGAAAAATTCGAGGAAATGGGATTCACTAAGATAT
GGGACCCTGATAAGGCTGTTCTTATATATGACCATCTTGTTCCAGCAAGTCAGGTTGATGATACAAGACATTTCCGTATT
GGTAATGAATTTGTGGAAGAATACGGAATGAAGAATATCCACCGTTCAGACGGAATCTGCCATCAGTTAATGACAGAGGC
AGGTTATGTAAAGCCGGGAGATATCGCATTTGGTACAGACAGCCACACAACAACATACGGCTGTGTTGGTGCATTTTCAT
CAGGTATCGGATATACAGAGATGGCAAGTGTATTAGGAACAGGCAAAATGTGGGTTAAAGTTCCACAGACTATCAAGGTA
ACAGTTAATGGAAAGCTTCCTGAAAATGTGCGTTCAAAGGATATCATATTAAGACTTATCGGTGACCTTGGAGCAGATGG
CGCAACTTATCAGGCACTTGAATTCACAGGTTCAACAATTGACGAGATGAGCGTTGCAAGCCGTATGACTATGTCTAACA
TGGCTATTGAAGCCGGTGCCAAATGTGCTCTTTTTACACCAGATGAAAAGACAGCAGAGTACTGTAATGTAGAACTTACA
GATGAATTAAAGGATTTAAAGGGAGATGCGGATGCAGTATATGCAAGAGAGATTACATATAATGCAGAGGATTTAGTTCC
AGTTATTGCATGTCCATCACAGGTAGATAACATCAAGCCTGTTACAGAGCTTGCCGGAACAGCAGTAGATCAGGTATTCA
TCGGTTCATGTACTAATGGAAGATTAGAGGATCTTCAGTGTGCAGCAGCTATTCTTAAGGGTAAGAAGGTTGCACCATTT
GTCAAGTTAATAGTTACTCCTGCAAGCCGCAAGATATATAAAGAAGCTCTTGCAGACGGAACACTTGAGACACTTGTTGA
AGCCGGAGCAATTGTTACACATCCAGGATGCGGATTATGCTGTGGACGAACAGGCGGAATTCTTACTGACGGAGAGGTAG
TTGTTGCAACTAACAACAGAAACTTCCTTGGAAGAATGGGAACAAGCAAGGTTAAGATATTCCTTGCGTCTCCAGCTACA
GCAGCAGCAAGTGCTATTGCTGGAAAGATTGTGGAGGCTTAA

Upstream 100 bases:

>100_bases
CACTTCCTGATAATCTTGTGGATATACTTAATGCAGGCGGACTTGTTAAGGCAATGAGAAAGCTTAACGGTCTTGACTAA
GACAGGGAGAGGTAAGAATA

Downstream 100 bases:

>100_bases
AAACAGACAGACTTTACATAATTTCTGAAATTCTTAGACAATATAATATTGTGATAAAGGGAGGCATATTCTACGGAGGA
TATGCCTTCTATGTAATTAG

Product: 3-isopropylmalate/(R)-2-methylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MGHTIIEKIIGKNIGREVKPGDIVTVNVDRVMIHDIFIPFVKEKFEEMGFTKIWDPDKAVLIYDHLVPASQVDDTRHFRI
GNEFVEEYGMKNIHRSDGICHQLMTEAGYVKPGDIAFGTDSHTTTYGCVGAFSSGIGYTEMASVLGTGKMWVKVPQTIKV
TVNGKLPENVRSKDIILRLIGDLGADGATYQALEFTGSTIDEMSVASRMTMSNMAIEAGAKCALFTPDEKTAEYCNVELT
DELKDLKGDADAVYAREITYNAEDLVPVIACPSQVDNIKPVTELAGTAVDQVFIGSCTNGRLEDLQCAAAILKGKKVAPF
VKLIVTPASRKIYKEALADGTLETLVEAGAIVTHPGCGLCCGRTGGILTDGEVVVATNNRNFLGRMGTSKVKIFLASPAT
AAASAIAGKIVEA

Sequences:

>Translated_413_residues
MGHTIIEKIIGKNIGREVKPGDIVTVNVDRVMIHDIFIPFVKEKFEEMGFTKIWDPDKAVLIYDHLVPASQVDDTRHFRI
GNEFVEEYGMKNIHRSDGICHQLMTEAGYVKPGDIAFGTDSHTTTYGCVGAFSSGIGYTEMASVLGTGKMWVKVPQTIKV
TVNGKLPENVRSKDIILRLIGDLGADGATYQALEFTGSTIDEMSVASRMTMSNMAIEAGAKCALFTPDEKTAEYCNVELT
DELKDLKGDADAVYAREITYNAEDLVPVIACPSQVDNIKPVTELAGTAVDQVFIGSCTNGRLEDLQCAAAILKGKKVAPF
VKLIVTPASRKIYKEALADGTLETLVEAGAIVTHPGCGLCCGRTGGILTDGEVVVATNNRNFLGRMGTSKVKIFLASPAT
AAASAIAGKIVEA
>Mature_412_residues
GHTIIEKIIGKNIGREVKPGDIVTVNVDRVMIHDIFIPFVKEKFEEMGFTKIWDPDKAVLIYDHLVPASQVDDTRHFRIG
NEFVEEYGMKNIHRSDGICHQLMTEAGYVKPGDIAFGTDSHTTTYGCVGAFSSGIGYTEMASVLGTGKMWVKVPQTIKVT
VNGKLPENVRSKDIILRLIGDLGADGATYQALEFTGSTIDEMSVASRMTMSNMAIEAGAKCALFTPDEKTAEYCNVELTD
ELKDLKGDADAVYAREITYNAEDLVPVIACPSQVDNIKPVTELAGTAVDQVFIGSCTNGRLEDLQCAAAILKGKKVAPFV
KLIVTPASRKIYKEALADGTLETLVEAGAIVTHPGCGLCCGRTGGILTDGEVVVATNNRNFLGRMGTSKVKIFLASPATA
AASAIAGKIVEA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=447, Percent_Identity=29.5302013422819, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI8659555, Length=403, Percent_Identity=26.7990074441687, Blast_Score=102, Evalue=7e-22,
Organism=Escherichia coli, GI1786259, Length=467, Percent_Identity=33.4047109207709, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI2367097, Length=431, Percent_Identity=22.2737819025522, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787531, Length=390, Percent_Identity=25.6410256410256, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI87081781, Length=422, Percent_Identity=22.0379146919431, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25149337, Length=415, Percent_Identity=27.9518072289157, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI32564738, Length=347, Percent_Identity=29.1066282420749, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17568399, Length=394, Percent_Identity=26.3959390862944, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25149342, Length=284, Percent_Identity=28.5211267605634, Blast_Score=95, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6320440, Length=442, Percent_Identity=33.2579185520362, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6321429, Length=455, Percent_Identity=32.0879120879121, Blast_Score=208, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6323335, Length=448, Percent_Identity=29.6875, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6322261, Length=430, Percent_Identity=26.2790697674419, Blast_Score=131, Evalue=2e-31,
Organism=Drosophila melanogaster, GI28571643, Length=445, Percent_Identity=26.7415730337079, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI281365315, Length=419, Percent_Identity=28.4009546539379, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI17864292, Length=419, Percent_Identity=28.4009546539379, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI161076999, Length=351, Percent_Identity=29.6296296296296, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17137564, Length=371, Percent_Identity=26.1455525606469, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24645686, Length=369, Percent_Identity=25.7452574525745, Blast_Score=86, Evalue=6e-17,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 44333; Mature: 44202

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00450 ACONITASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHTIIEKIIGKNIGREVKPGDIVTVNVDRVMIHDIFIPFVKEKFEEMGFTKIWDPDKAV
CCHHHHHHHHHHHCCCCCCCCCEEEEEECCEEEHHHHHHHHHHHHHHCCCCEEECCCCEE
LIYDHLVPASQVDDTRHFRIGNEFVEEYGMKNIHRSDGICHQLMTEAGYVKPGDIAFGTD
EEEECCCCCCCCCCCCEEECCHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCEEECCC
SHTTTYGCVGAFSSGIGYTEMASVLGTGKMWVKVPQTIKVTVNGKLPENVRSKDIILRLI
CCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEECCEEEEEECCCCCCCCCHHHHHHHHH
GDLGADGATYQALEFTGSTIDEMSVASRMTMSNMAIEAGAKCALFTPDEKTAEYCNVELT
HHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHCCEEEH
DELKDLKGDADAVYAREITYNAEDLVPVIACPSQVDNIKPVTELAGTAVDQVFIGSCTNG
HHHHHCCCCCCEEEEEEEECCHHHCEEEEECCCCCCCCCHHHHHHHHHHHHHEEECCCCC
RLEDLQCAAAILKGKKVAPFVKLIVTPASRKIYKEALADGTLETLVEAGAIVTHPGCGLC
CHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCCHHHHHHHCCCEEECCCCCEE
CGRTGGILTDGEVVVATNNRNFLGRMGTSKVKIFLASPATAAASAIAGKIVEA
ECCCCCEEECCEEEEEECCCCEEECCCCCEEEEEEECCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
GHTIIEKIIGKNIGREVKPGDIVTVNVDRVMIHDIFIPFVKEKFEEMGFTKIWDPDKAV
CHHHHHHHHHHHCCCCCCCCCEEEEEECCEEEHHHHHHHHHHHHHHCCCCEEECCCCEE
LIYDHLVPASQVDDTRHFRIGNEFVEEYGMKNIHRSDGICHQLMTEAGYVKPGDIAFGTD
EEEECCCCCCCCCCCCEEECCHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCEEECCC
SHTTTYGCVGAFSSGIGYTEMASVLGTGKMWVKVPQTIKVTVNGKLPENVRSKDIILRLI
CCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEECCEEEEEECCCCCCCCCHHHHHHHHH
GDLGADGATYQALEFTGSTIDEMSVASRMTMSNMAIEAGAKCALFTPDEKTAEYCNVELT
HHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHCCEEEH
DELKDLKGDADAVYAREITYNAEDLVPVIACPSQVDNIKPVTELAGTAVDQVFIGSCTNG
HHHHHCCCCCCEEEEEEEECCHHHCEEEEECCCCCCCCCHHHHHHHHHHHHHEEECCCCC
RLEDLQCAAAILKGKKVAPFVKLIVTPASRKIYKEALADGTLETLVEAGAIVTHPGCGLC
CHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCCHHHHHHHCCCEEECCCCCEE
CGRTGGILTDGEVVVATNNRNFLGRMGTSKVKIFLASPATAAASAIAGKIVEA
ECCCCCEEECCEEEEEECCCCEEECCCCCEEEEEEECCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]