Click here to switch to the map view.

The map label for this gene is leuC [H]

Identifier: 238917293

GI number: 238917293

Start: 1407829

End: 1409589

Strand: Reverse

Name: leuC [H]

Synonym: EUBELI_01368

Alternate gene names: 238917293

Gene position: 1409589-1407829 (Counterclockwise)

Preceding gene: 238917294

Following gene: 238917292

Centisome position: 65.74

GC content: 41.17

Gene sequence:

>1761_bases
ATGGGTAGGATATTTAAGTTTGATAAAGATGTTGATACAGATCAGATTATTGCATCGCAGTATCTTTTGTTTCCGACAAT
TGATGAGATGAAGAGTCATACATTTGAATCATTAGACTCTGATTTTGCTTCTGCCGTAAAGCCGGGAGATTTCGTTGTTG
CAGATGACAACTTTGGATGTGGTTCATCAAGAGAGCAGGCACCAAGCGTACTTAAGGCATTAGGTGTCAAGGCAGTGATT
GCCAAATCATTTGCAAGAATATTCTATCGTAATGCTATTAATATAGGACTTCCTGTTATTGTATGTAAAGAACTCCATGA
CGAAGTTAATGCGGGGGATGAATGTGAGCTTTCATTAGAAGATGGAATTATAACTGTTAATGGAAAGACTTATACATGTA
CTAAGCTTCCAGCCAAGATGCAGGCTATTCTTAATCAGGGTGGTCTTATTGCATCACTTGATGGTGAGAAAGAAGAGTCT
GAGAGTACAGCCGTACCTGCAGATGAGGCAAAGCATGGCATGACTATTGCAGAGAAGATTATCGCAAGGGCAGCAGGTCT
TTCACAGGTTAAGGCTGGTGATATTGCAACTGTTACTCTTGACAGACTTATGAGTAATGATGGAACAACACATCTTACTA
TTGGTATGTATGAGAAGTTAAAGAATCCTCATATTGCTGACAAGGATAAGCTTGTATGGATTGTAGACCACAATATTCCA
TCAGACAGTCCTAAGACAGCAGCCTCACAGAAGAAGATGAGAGACTTTGCTAAGGCTAATGATATTAAGTTCTACGAGGG
CGAGGGTGTGTGTCATCAGGTTATGATGGAAAATCATGTAGTGCCGGGTGAGCTTATATTTGGAGCAGACAGCCATACTT
GTGCATATGGTGCGTTAGGTGCATTTGGTACAGGTGTTGGTTGTACAGATTATTTGTATGCAATGGTTACAGGAACATCA
TGGGTAATGGTTCCGGGAACATTAAGATTCAACCTTAAGGGAAAGCTTAACGATGGAGTATATGCAAGAGATTTAATACT
CTCTATTATTGGAAAGATTGGCGCTAACGGTGCTAACTATAAAGCAATGGAATTTGCAGGAGAAGGCTTACACAGCCTTT
CAATGGCAGACAGAATATCAATCTGTAACCTTTGTGTAGAGGCAGGTGCTAAGACTGCACTCATGGAAGTTGATGATGTT
GCAATGGATTATCTTAAGGAGCATGGCAGAGAGCCAAAGGCATGCTTTACATCAGATGATGATGCAGTATTTGAGCAGGT
TTATGATATTGACCTTTCAACAATACAGCCAATTGTTGCAAAGCCACATTTTGTTGACAATGTAGTTCCGGCTAAGGAAT
CACTTGGTGTAAAGATTGATGAAGCATTTCTTGGTTCATGTAACAATGGACGAATCGAGGATTTAAGAGTTGGCGCAGCG
ATCATCAAAGGGAAAAAGGTTGCTCCTAAGGTAAGATTCCTTGTAGTTCCTGCAAGCCGTTCGGTATATCTTCAGGCTAT
GAAAGAGGGACTCCTTGATATATTCATGGACGCAGGTGCAATTGTTATGAATCCTAACTGCTCTGTATGCTGGGGAAGCT
GTCAGGGTGTTATCGGTGAGGGTGAGACTCTTATCAGTACAGGTACACGAAACTTCAAGGGACGTGCAGGACATAAAGAT
TCTTTTGTATATCTTGCTTCAGCGGCTACTGTTACAGCATCAGCAATCAAGGGCGAGATAGCAACAGCAGACATGGTATA
A

Upstream 100 bases:

>100_bases
TGTTAATATTAACGATTGAAAAACACACTTTAATAGGGTAAAATGTTAAAATGACTATTAATAATACCTGTTATATCAGG
GATAAGATAAGGAGAAAACA

Downstream 100 bases:

>100_bases
GACAAGGATTTAACTTCAAAGGAGTAATTATGAACGAGAAATTTAGCGGTAAAGTATGGGTACTTGGTGATGACATTGAT
ACAGATATCATTATCCCTAC

Product: 3-isopropylmalate/(R)-2-methylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 586; Mature: 585

Protein sequence:

>586_residues
MGRIFKFDKDVDTDQIIASQYLLFPTIDEMKSHTFESLDSDFASAVKPGDFVVADDNFGCGSSREQAPSVLKALGVKAVI
AKSFARIFYRNAINIGLPVIVCKELHDEVNAGDECELSLEDGIITVNGKTYTCTKLPAKMQAILNQGGLIASLDGEKEES
ESTAVPADEAKHGMTIAEKIIARAAGLSQVKAGDIATVTLDRLMSNDGTTHLTIGMYEKLKNPHIADKDKLVWIVDHNIP
SDSPKTAASQKKMRDFAKANDIKFYEGEGVCHQVMMENHVVPGELIFGADSHTCAYGALGAFGTGVGCTDYLYAMVTGTS
WVMVPGTLRFNLKGKLNDGVYARDLILSIIGKIGANGANYKAMEFAGEGLHSLSMADRISICNLCVEAGAKTALMEVDDV
AMDYLKEHGREPKACFTSDDDAVFEQVYDIDLSTIQPIVAKPHFVDNVVPAKESLGVKIDEAFLGSCNNGRIEDLRVGAA
IIKGKKVAPKVRFLVVPASRSVYLQAMKEGLLDIFMDAGAIVMNPNCSVCWGSCQGVIGEGETLISTGTRNFKGRAGHKD
SFVYLASAATVTASAIKGEIATADMV

Sequences:

>Translated_586_residues
MGRIFKFDKDVDTDQIIASQYLLFPTIDEMKSHTFESLDSDFASAVKPGDFVVADDNFGCGSSREQAPSVLKALGVKAVI
AKSFARIFYRNAINIGLPVIVCKELHDEVNAGDECELSLEDGIITVNGKTYTCTKLPAKMQAILNQGGLIASLDGEKEES
ESTAVPADEAKHGMTIAEKIIARAAGLSQVKAGDIATVTLDRLMSNDGTTHLTIGMYEKLKNPHIADKDKLVWIVDHNIP
SDSPKTAASQKKMRDFAKANDIKFYEGEGVCHQVMMENHVVPGELIFGADSHTCAYGALGAFGTGVGCTDYLYAMVTGTS
WVMVPGTLRFNLKGKLNDGVYARDLILSIIGKIGANGANYKAMEFAGEGLHSLSMADRISICNLCVEAGAKTALMEVDDV
AMDYLKEHGREPKACFTSDDDAVFEQVYDIDLSTIQPIVAKPHFVDNVVPAKESLGVKIDEAFLGSCNNGRIEDLRVGAA
IIKGKKVAPKVRFLVVPASRSVYLQAMKEGLLDIFMDAGAIVMNPNCSVCWGSCQGVIGEGETLISTGTRNFKGRAGHKD
SFVYLASAATVTASAIKGEIATADMV
>Mature_585_residues
GRIFKFDKDVDTDQIIASQYLLFPTIDEMKSHTFESLDSDFASAVKPGDFVVADDNFGCGSSREQAPSVLKALGVKAVIA
KSFARIFYRNAINIGLPVIVCKELHDEVNAGDECELSLEDGIITVNGKTYTCTKLPAKMQAILNQGGLIASLDGEKEESE
STAVPADEAKHGMTIAEKIIARAAGLSQVKAGDIATVTLDRLMSNDGTTHLTIGMYEKLKNPHIADKDKLVWIVDHNIPS
DSPKTAASQKKMRDFAKANDIKFYEGEGVCHQVMMENHVVPGELIFGADSHTCAYGALGAFGTGVGCTDYLYAMVTGTSW
VMVPGTLRFNLKGKLNDGVYARDLILSIIGKIGANGANYKAMEFAGEGLHSLSMADRISICNLCVEAGAKTALMEVDDVA
MDYLKEHGREPKACFTSDDDAVFEQVYDIDLSTIQPIVAKPHFVDNVVPAKESLGVKIDEAFLGSCNNGRIEDLRVGAAI
IKGKKVAPKVRFLVVPASRSVYLQAMKEGLLDIFMDAGAIVMNPNCSVCWGSCQGVIGEGETLISTGTRNFKGRAGHKDS
FVYLASAATVTASAIKGEIATADMV

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=341, Percent_Identity=29.3255131964809, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI8659555, Length=447, Percent_Identity=25.503355704698, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI41352693, Length=395, Percent_Identity=25.8227848101266, Blast_Score=87, Evalue=6e-17,
Organism=Escherichia coli, GI1786259, Length=357, Percent_Identity=34.453781512605, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI87081781, Length=355, Percent_Identity=28.7323943661972, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI2367097, Length=431, Percent_Identity=24.5939675174014, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1787531, Length=389, Percent_Identity=25.9640102827763, Blast_Score=96, Evalue=9e-21,
Organism=Escherichia coli, GI1786258, Length=156, Percent_Identity=35.2564102564103, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25149337, Length=469, Percent_Identity=27.7185501066098, Blast_Score=145, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI32564738, Length=344, Percent_Identity=30.2325581395349, Blast_Score=140, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI25149342, Length=280, Percent_Identity=28.9285714285714, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17568399, Length=399, Percent_Identity=25.8145363408521, Blast_Score=98, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320440, Length=452, Percent_Identity=32.5221238938053, Blast_Score=203, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6321429, Length=387, Percent_Identity=31.266149870801, Blast_Score=181, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6322261, Length=455, Percent_Identity=28.3516483516484, Blast_Score=171, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6323335, Length=417, Percent_Identity=29.7362110311751, Blast_Score=167, Evalue=5e-42,
Organism=Drosophila melanogaster, GI281365315, Length=447, Percent_Identity=25.9507829977629, Blast_Score=129, Evalue=8e-30,
Organism=Drosophila melanogaster, GI17864292, Length=447, Percent_Identity=25.9507829977629, Blast_Score=129, Evalue=8e-30,
Organism=Drosophila melanogaster, GI161076999, Length=370, Percent_Identity=27.5675675675676, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI28571643, Length=448, Percent_Identity=26.3392857142857, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24645686, Length=403, Percent_Identity=24.8138957816377, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17137564, Length=401, Percent_Identity=23.9401496259352, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251
- InterPro:   IPR011823 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 62903; Mature: 62771

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRIFKFDKDVDTDQIIASQYLLFPTIDEMKSHTFESLDSDFASAVKPGDFVVADDNFGC
CCCCCCCCCCCCHHHHHHHCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
GSSREQAPSVLKALGVKAVIAKSFARIFYRNAINIGLPVIVCKELHDEVNAGDECELSLE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEEC
DGIITVNGKTYTCTKLPAKMQAILNQGGLIASLDGEKEESESTAVPADEAKHGMTIAEKI
CCEEEECCCEEEEECCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHCCHHHHHHH
IARAAGLSQVKAGDIATVTLDRLMSNDGTTHLTIGMYEKLKNPHIADKDKLVWIVDHNIP
HHHHCCCCCCCCCCEEHEEHHHHHCCCCCEEEEEEHHHHHCCCCCCCCCCEEEEEECCCC
SDSPKTAASQKKMRDFAKANDIKFYEGEGVCHQVMMENHVVPGELIFGADSHTCAYGALG
CCCCCHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHCCCCCCCEEECCCCCCCHHHCHH
AFGTGVGCTDYLYAMVTGTSWVMVPGTLRFNLKGKLNDGVYARDLILSIIGKIGANGANY
HCCCCCCHHHHHHHHCCCCEEEEECCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCE
KAMEFAGEGLHSLSMADRISICNLCVEAGAKTALMEVDDVAMDYLKEHGREPKACFTSDD
EHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCCEECCCC
DAVFEQVYDIDLSTIQPIVAKPHFVDNVVPAKESLGVKIDEAFLGSCNNGRIEDLRVGAA
HHHHHHHHHCCHHHCCHHHCCCCHHCCCCCCHHHCCCEECHHHHCCCCCCCCHHEEHHHH
IIKGKKVAPKVRFLVVPASRSVYLQAMKEGLLDIFMDAGAIVMNPNCSVCWGSCQGVIGE
HHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEEECCCCCCCCC
GETLISTGTRNFKGRAGHKDSFVYLASAATVTASAIKGEIATADMV
CCEEEECCCCCCCCCCCCCCCEEEEEEHHHHHHHHHCCCCEECCCC
>Mature Secondary Structure 
GRIFKFDKDVDTDQIIASQYLLFPTIDEMKSHTFESLDSDFASAVKPGDFVVADDNFGC
CCCCCCCCCCCHHHHHHHCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
GSSREQAPSVLKALGVKAVIAKSFARIFYRNAINIGLPVIVCKELHDEVNAGDECELSLE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEEC
DGIITVNGKTYTCTKLPAKMQAILNQGGLIASLDGEKEESESTAVPADEAKHGMTIAEKI
CCEEEECCCEEEEECCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHCCHHHHHHH
IARAAGLSQVKAGDIATVTLDRLMSNDGTTHLTIGMYEKLKNPHIADKDKLVWIVDHNIP
HHHHCCCCCCCCCCEEHEEHHHHHCCCCCEEEEEEHHHHHCCCCCCCCCCEEEEEECCCC
SDSPKTAASQKKMRDFAKANDIKFYEGEGVCHQVMMENHVVPGELIFGADSHTCAYGALG
CCCCCHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHCCCCCCCEEECCCCCCCHHHCHH
AFGTGVGCTDYLYAMVTGTSWVMVPGTLRFNLKGKLNDGVYARDLILSIIGKIGANGANY
HCCCCCCHHHHHHHHCCCCEEEEECCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCE
KAMEFAGEGLHSLSMADRISICNLCVEAGAKTALMEVDDVAMDYLKEHGREPKACFTSDD
EHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCCEECCCC
DAVFEQVYDIDLSTIQPIVAKPHFVDNVVPAKESLGVKIDEAFLGSCNNGRIEDLRVGAA
HHHHHHHHHCCHHHCCHHHCCCCHHCCCCCCHHHCCCEECHHHHCCCCCCCCHHEEHHHH
IIKGKKVAPKVRFLVVPASRSVYLQAMKEGLLDIFMDAGAIVMNPNCSVCWGSCQGVIGE
HHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEEECCCCCCCCC
GETLISTGTRNFKGRAGHKDSFVYLASAATVTASAIKGEIATADMV
CCEEEECCCCCCCCCCCCCCCEEEEEEHHHHHHHHHCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA