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The map label for this gene is ptsI [H]

Identifier: 238917199

GI number: 238917199

Start: 1307965

End: 1309692

Strand: Reverse

Name: ptsI [H]

Synonym: EUBELI_01272

Alternate gene names: 238917199

Gene position: 1309692-1307965 (Counterclockwise)

Preceding gene: 238917200

Following gene: 238917198

Centisome position: 61.08

GC content: 38.02

Gene sequence:

>1728_bases
ATGGAAAGAGGAATAGGAACTTCAAGAGGAATTGGCATAGGACATGTTCTTATTGTTTATAATGCCAGTGCTGTTGTAAA
CAGGGGAACGCATTATAATGCCGAAGAAGAGAAGAAAAGATTCTTTGAGGCCAGAAAAACATTTATAGAGCAGACGGAAG
AATTATTGTCTGAACTGGAGAAAAAACTTGGTGAGTCTGACAAGGATGCACTGGTGCTTAAGAATCAGATATATCTTGCA
AGAGATATAACAACAGAGAATGAAGTTGTTGCTGCAATTGATAATAGTCATATATGTGCAGAAGCAGCTTTTGATGATGT
GTGTAATAAGCTGATTCAATTGTTTTCTTCAATGGATAATCCTGATATGCAGCAGCGTGTTACTGATATTCAGGATATGA
GAGAAAGAATGATACAGATATTGCAGGGAACGAAAGCATTTGACCTTACTAATCTTCCGGATAATACTGTTATTGTTGCA
GATGAGATTAAGCCATCGATGACAGCTTCTATGGACATAGCACATGTTGCAGGTATTATAGCTGAAAAGGGTGGCGATAC
GGCGCATGCTTCAATTCTTGCAAGAGCACTTGAGATTCCGGCAGTACTTAGTGTGAAGGGAATACTTCAGAAAGTAAAAG
ATGGAGATTTTATAATAATAGATGGAGCTTATGGAGAAGTTTTTATTAATCCGACACCGCGTACACTTTCTATATATGAG
AAGAAAAAGAAAAAATATGAAGAACATATTGAGGAATTAAAGACCTTTATTAATAAAAGCACAGAGACAGCTGATGGAAA
GAAGGTTATGCTTGCTGCCAATATTGGTGGAGCTGATGAAGCAGCCAAGGCAGTTAAGGACGGAGCTGAAGGCGTGGGAC
TTTTCAGGACAGAGTTTCTCTTTCTTAATAAGAATGCGCTGCCGACAGAGGAGGAACAGTTTGAGGAATATAAGAAGGCA
GCAGTACTGACAAAAGGCATGCCACTTACAATAAGAACACTTGATATTGGTGGAGATAAGGATATTCCTTATATGGGACT
TACCAAAGAGGCTAATCCATTTTTAGGATACCGTGCAATAAGATTCTGCCTTGACAGAGTTGATATATTTACAACACAGC
TTCGTGCAATTCTAAGGGCAAGTGCATATGGCTGTATAAGAATTATGATACCTATGATTACATCAGTTAATGAAGTAAGA
CAGGTGAAAGAGCTGATTAAGAAGATATGCAGCGACCTTGACCGTAACGGAATTAATTATGATAAGAATATCCAGACAGG
CATTATGATTGAAACTCCGGCAGCTGCTGTTATGGCAGATACATTAGCAAAGGAGGCTGATTTCTTTTCAATTGGTACTA
ATGACCTTACACAGTATACTATTGCAGTAGACAGATGCAATGAAAATGTGGCATATCTGTATTCTGCATTTAATCCGGCA
GTATTAAGGCTTATAAGAAGGATAATAGAATGTGCCCATAATGCAGGTATTGAAGCCGGAATGTGTGGTGAGGCAGCTGC
AAATGCTTTAATGGCTCCGATACTTTTATCATTTGGTCTGGATGAATTTTCAGTATCAACAGGAAAGGTATTAGAGACGA
GAAAGACAATTGCAGACTGGTCAATTAAAGAAGCCGGACTTGTAACAGATAAGGTTATGTCAATGGTAACTGAGAAAGAA
GTAACAGACTATCTTAGTGACTATATATCAGAAAGAAATAAAAAGTAA

Upstream 100 bases:

>100_bases
TCCGGTGCTTGCAAGGGAGACAATATATTCGAGAGATACATCGGAAACTATTGTTAACATGATAAGGTATGTGTTTAATC
AGTAGAATGGGGGCATATGT

Downstream 100 bases:

>100_bases
AAGGAAATATATATATGGCAGTTTATGAGACAGATGAGATTATCGAGAGAGTTATTCTGGTTGGAGTAGCATTTGATACA
GATGATGATACAGAACGCTC

Product: hypothetical protein

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MERGIGTSRGIGIGHVLIVYNASAVVNRGTHYNAEEEKKRFFEARKTFIEQTEELLSELEKKLGESDKDALVLKNQIYLA
RDITTENEVVAAIDNSHICAEAAFDDVCNKLIQLFSSMDNPDMQQRVTDIQDMRERMIQILQGTKAFDLTNLPDNTVIVA
DEIKPSMTASMDIAHVAGIIAEKGGDTAHASILARALEIPAVLSVKGILQKVKDGDFIIIDGAYGEVFINPTPRTLSIYE
KKKKKYEEHIEELKTFINKSTETADGKKVMLAANIGGADEAAKAVKDGAEGVGLFRTEFLFLNKNALPTEEEQFEEYKKA
AVLTKGMPLTIRTLDIGGDKDIPYMGLTKEANPFLGYRAIRFCLDRVDIFTTQLRAILRASAYGCIRIMIPMITSVNEVR
QVKELIKKICSDLDRNGINYDKNIQTGIMIETPAAAVMADTLAKEADFFSIGTNDLTQYTIAVDRCNENVAYLYSAFNPA
VLRLIRRIIECAHNAGIEAGMCGEAAANALMAPILLSFGLDEFSVSTGKVLETRKTIADWSIKEAGLVTDKVMSMVTEKE
VTDYLSDYISERNKK

Sequences:

>Translated_575_residues
MERGIGTSRGIGIGHVLIVYNASAVVNRGTHYNAEEEKKRFFEARKTFIEQTEELLSELEKKLGESDKDALVLKNQIYLA
RDITTENEVVAAIDNSHICAEAAFDDVCNKLIQLFSSMDNPDMQQRVTDIQDMRERMIQILQGTKAFDLTNLPDNTVIVA
DEIKPSMTASMDIAHVAGIIAEKGGDTAHASILARALEIPAVLSVKGILQKVKDGDFIIIDGAYGEVFINPTPRTLSIYE
KKKKKYEEHIEELKTFINKSTETADGKKVMLAANIGGADEAAKAVKDGAEGVGLFRTEFLFLNKNALPTEEEQFEEYKKA
AVLTKGMPLTIRTLDIGGDKDIPYMGLTKEANPFLGYRAIRFCLDRVDIFTTQLRAILRASAYGCIRIMIPMITSVNEVR
QVKELIKKICSDLDRNGINYDKNIQTGIMIETPAAAVMADTLAKEADFFSIGTNDLTQYTIAVDRCNENVAYLYSAFNPA
VLRLIRRIIECAHNAGIEAGMCGEAAANALMAPILLSFGLDEFSVSTGKVLETRKTIADWSIKEAGLVTDKVMSMVTEKE
VTDYLSDYISERNKK
>Mature_575_residues
MERGIGTSRGIGIGHVLIVYNASAVVNRGTHYNAEEEKKRFFEARKTFIEQTEELLSELEKKLGESDKDALVLKNQIYLA
RDITTENEVVAAIDNSHICAEAAFDDVCNKLIQLFSSMDNPDMQQRVTDIQDMRERMIQILQGTKAFDLTNLPDNTVIVA
DEIKPSMTASMDIAHVAGIIAEKGGDTAHASILARALEIPAVLSVKGILQKVKDGDFIIIDGAYGEVFINPTPRTLSIYE
KKKKKYEEHIEELKTFINKSTETADGKKVMLAANIGGADEAAKAVKDGAEGVGLFRTEFLFLNKNALPTEEEQFEEYKKA
AVLTKGMPLTIRTLDIGGDKDIPYMGLTKEANPFLGYRAIRFCLDRVDIFTTQLRAILRASAYGCIRIMIPMITSVNEVR
QVKELIKKICSDLDRNGINYDKNIQTGIMIETPAAAVMADTLAKEADFFSIGTNDLTQYTIAVDRCNENVAYLYSAFNPA
VLRLIRRIIECAHNAGIEAGMCGEAAANALMAPILLSFGLDEFSVSTGKVLETRKTIADWSIKEAGLVTDKVMSMVTEKE
VTDYLSDYISERNKK

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=575, Percent_Identity=38.9565217391304, Blast_Score=423, Evalue=1e-119,
Organism=Escherichia coli, GI48994992, Length=516, Percent_Identity=35.2713178294574, Blast_Score=320, Evalue=1e-88,
Organism=Escherichia coli, GI1788726, Length=528, Percent_Identity=35.4166666666667, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1789193, Length=502, Percent_Identity=31.4741035856574, Blast_Score=267, Evalue=1e-72,
Organism=Escherichia coli, GI1787994, Length=441, Percent_Identity=26.984126984127, Blast_Score=116, Evalue=4e-27,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63653; Mature: 63653

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERGIGTSRGIGIGHVLIVYNASAVVNRGTHYNAEEEKKRFFEARKTFIEQTEELLSELE
CCCCCCCCCCCCCEEEEEEEECHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KKLGESDKDALVLKNQIYLARDITTENEVVAAIDNSHICAEAAFDDVCNKLIQLFSSMDN
HHHCCCCCCEEEEECCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCC
PDMQQRVTDIQDMRERMIQILQGTKAFDLTNLPDNTVIVADEIKPSMTASMDIAHVAGII
CHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
AEKGGDTAHASILARALEIPAVLSVKGILQKVKDGDFIIIDGAYGEVFINPTPRTLSIYE
HHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCHHHHH
KKKKKYEEHIEELKTFINKSTETADGKKVMLAANIGGADEAAKAVKDGAEGVGLFRTEFL
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHHEEE
FLNKNALPTEEEQFEEYKKAAVLTKGMPLTIRTLDIGGDKDIPYMGLTKEANPFLGYRAI
EEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHH
RFCLDRVDIFTTQLRAILRASAYGCIRIMIPMITSVNEVRQVKELIKKICSDLDRNGINY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCC
DKNIQTGIMIETPAAAVMADTLAKEADFFSIGTNDLTQYTIAVDRCNENVAYLYSAFNPA
CCCCCCCEEEECCHHHHHHHHHHHCCCEEECCCCCHHEEEEEEECCCCCCEEHHHHCCHH
VLRLIRRIIECAHNAGIEAGMCGEAAANALMAPILLSFGLDEFSVSTGKVLETRKTIADW
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHCC
SIKEAGLVTDKVMSMVTEKEVTDYLSDYISERNKK
CCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MERGIGTSRGIGIGHVLIVYNASAVVNRGTHYNAEEEKKRFFEARKTFIEQTEELLSELE
CCCCCCCCCCCCCEEEEEEEECHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KKLGESDKDALVLKNQIYLARDITTENEVVAAIDNSHICAEAAFDDVCNKLIQLFSSMDN
HHHCCCCCCEEEEECCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCC
PDMQQRVTDIQDMRERMIQILQGTKAFDLTNLPDNTVIVADEIKPSMTASMDIAHVAGII
CHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
AEKGGDTAHASILARALEIPAVLSVKGILQKVKDGDFIIIDGAYGEVFINPTPRTLSIYE
HHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCHHHHH
KKKKKYEEHIEELKTFINKSTETADGKKVMLAANIGGADEAAKAVKDGAEGVGLFRTEFL
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHHEEE
FLNKNALPTEEEQFEEYKKAAVLTKGMPLTIRTLDIGGDKDIPYMGLTKEANPFLGYRAI
EEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHH
RFCLDRVDIFTTQLRAILRASAYGCIRIMIPMITSVNEVRQVKELIKKICSDLDRNGINY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCC
DKNIQTGIMIETPAAAVMADTLAKEADFFSIGTNDLTQYTIAVDRCNENVAYLYSAFNPA
CCCCCCCEEEECCHHHHHHHHHHHCCCEEECCCCCHHEEEEEEECCCCCCEEHHHHCCHH
VLRLIRRIIECAHNAGIEAGMCGEAAANALMAPILLSFGLDEFSVSTGKVLETRKTIADW
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHCC
SIKEAGLVTDKVMSMVTEKEVTDYLSDYISERNKK
CCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA