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The map label for this gene is ilvB [H]

Identifier: 238916701

GI number: 238916701

Start: 773560

End: 775242

Strand: Direct

Name: ilvB [H]

Synonym: EUBELI_00763

Alternate gene names: 238916701

Gene position: 773560-775242 (Clockwise)

Preceding gene: 238916700

Following gene: 238916702

Centisome position: 36.08

GC content: 39.69

Gene sequence:

>1683_bases
ATGAAGTTAAATGGTTCAGAGATAATTGTTGAATGCTTGAAAGAACAGGGCGTTGATACAATATTTGGATACCCTGGTGG
AGCTATTCTTAATGTATATGATGCTCTTTATAAGCATTCAGATGAGATAACTCATATTCTTACATCACATGAGCAGGGTG
CATCGCACGCTGCTGATGGATATGCTAGGGCAACAGGTAAGGTTGGTGTATGTTTTGCAACATCAGGTCCAGGAGCGACT
AACCTTGTAACAGGTATTGCAACTGCATATATGGATTCAGTTCCTATGGTTGCAATTACATGTAATGTAACAGTTCCTCT
TCTTGGAAGAGACAGCTTCCAGGAGATAGATATAGCGGGTGTTACAATGCCAATTACAAAGCACAGCTATATTGTTAAGA
ATGTAAAGGATCTTGCCAAGACATTAAGAAATGCTTTCAGAATTGCCAAGGAAGGAAGACCGGGTCCGGTATTAGTTGAT
GTTACTAAGGATGTAACAGCAGCAGAGTGTGAGTACACACCAAGAGTTCCAAAACCTGTAGAGAGAATTACAGCAAGCAT
TAAGGAAGAAGATATTGACAAGGCTGTGACACTTATTAAGAAATCTAAGAAGCCATATGTATTCGTTGGTGGCGGAGCTG
TTATATCAGAGGCATCTGATGAACTTAAGAAGTTTGTTGAGAAGGTTGATGCACCGGTATGTGACTCACTTATGGGTAAG
GGCGCATTTGACGGATCTAATGAAAGATACACAGGAATGCTTGGTATGCATGGTACTAAGACATCTAACTACGGAGTTTC
AGAGTGTGATCTTCTTATTGTGGTTGGTGCAAGATTCTCAGACCGTGTTACTGGAAATGCTAAGAAATTCGCTAAGAATG
CCAAGATTCTCCAGTTTGATGTTGATGCAGCTGAGATTAACAAGAATGTCAGGACAGATGCACATGTACTCGGAGATGCT
AAAGAGATTCTTAAGAGAATCAATGATAAGTTAGATAATCAGGATCACTCAGACTGGATGGAACACATCAAGTCTTATAA
GCTTGATTATCCTATGAGATATAACAGAGATATTCTTAACGGACCTCTTATCATGGAGAAGATATACGAGATTACAAATG
GTGATGCAATTATTACAACAGATGTTGGACAGCATCAGATGTGGGCTGCACAGCATTATAAGTTCAAAGAGCCAAGAACA
CTTCTTACATCAGGCGGACTTGGAACTATGGGATATGGTCTTGGTGCATGTATAGGTGCCAAGGTTGGACGTAAGGATAA
GACAGTTGTTAATATTGCAGGTGATGGCTGCTTCCGTATGAATATGAATGAAGTTGCAACAGCAACCAGATATAACATTC
CTATTATTGAAGTTATATTTAATAACCATGTACTTGGTATGGTTAGACAGTGGCAGGATCTTTTCTATGGCAAGAGATAT
TCAGCTACAGTTCTTGATGATCAGGTGGATTTTGTTAAGGTTTCAGAAGGAATGGGAGCCAAGGCTTACAGTGTTGATAC
AATAGAGGAGTTTGAGAAAGCATTTAAAGAAGCAATCGAACTCAATATTCCATGTGTTATTGACTGTCATATTGACAGAG
AAGACAAGGTATTCCCTATGGTTTCACCGGGGGCTGCAATCTCAGAGGCATTCGACAGAGAAGACCTTAATAACAAGAAA
TAA

Upstream 100 bases:

>100_bases
TTACATCAGGAAACAGAGGAGCTATTCTTAAAAGTTCAGCAGATGAGTAATATTTAATATAATTATAAAAAGACATTAAA
GAGAAATCAGGAGAAGATAA

Downstream 100 bases:

>100_bases
TTCATATATTGACTTTAATGTGATAAAGGACTAAAATAGTTCTTAATATTTTATATTTTAGGAGGAAGAAAAGGTGAGTA
GAGTTTATAACTTTAGCGCT

Product: acetolactate synthase

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MKLNGSEIIVECLKEQGVDTIFGYPGGAILNVYDALYKHSDEITHILTSHEQGASHAADGYARATGKVGVCFATSGPGAT
NLVTGIATAYMDSVPMVAITCNVTVPLLGRDSFQEIDIAGVTMPITKHSYIVKNVKDLAKTLRNAFRIAKEGRPGPVLVD
VTKDVTAAECEYTPRVPKPVERITASIKEEDIDKAVTLIKKSKKPYVFVGGGAVISEASDELKKFVEKVDAPVCDSLMGK
GAFDGSNERYTGMLGMHGTKTSNYGVSECDLLIVVGARFSDRVTGNAKKFAKNAKILQFDVDAAEINKNVRTDAHVLGDA
KEILKRINDKLDNQDHSDWMEHIKSYKLDYPMRYNRDILNGPLIMEKIYEITNGDAIITTDVGQHQMWAAQHYKFKEPRT
LLTSGGLGTMGYGLGACIGAKVGRKDKTVVNIAGDGCFRMNMNEVATATRYNIPIIEVIFNNHVLGMVRQWQDLFYGKRY
SATVLDDQVDFVKVSEGMGAKAYSVDTIEEFEKAFKEAIELNIPCVIDCHIDREDKVFPMVSPGAAISEAFDREDLNNKK

Sequences:

>Translated_560_residues
MKLNGSEIIVECLKEQGVDTIFGYPGGAILNVYDALYKHSDEITHILTSHEQGASHAADGYARATGKVGVCFATSGPGAT
NLVTGIATAYMDSVPMVAITCNVTVPLLGRDSFQEIDIAGVTMPITKHSYIVKNVKDLAKTLRNAFRIAKEGRPGPVLVD
VTKDVTAAECEYTPRVPKPVERITASIKEEDIDKAVTLIKKSKKPYVFVGGGAVISEASDELKKFVEKVDAPVCDSLMGK
GAFDGSNERYTGMLGMHGTKTSNYGVSECDLLIVVGARFSDRVTGNAKKFAKNAKILQFDVDAAEINKNVRTDAHVLGDA
KEILKRINDKLDNQDHSDWMEHIKSYKLDYPMRYNRDILNGPLIMEKIYEITNGDAIITTDVGQHQMWAAQHYKFKEPRT
LLTSGGLGTMGYGLGACIGAKVGRKDKTVVNIAGDGCFRMNMNEVATATRYNIPIIEVIFNNHVLGMVRQWQDLFYGKRY
SATVLDDQVDFVKVSEGMGAKAYSVDTIEEFEKAFKEAIELNIPCVIDCHIDREDKVFPMVSPGAAISEAFDREDLNNKK
>Mature_560_residues
MKLNGSEIIVECLKEQGVDTIFGYPGGAILNVYDALYKHSDEITHILTSHEQGASHAADGYARATGKVGVCFATSGPGAT
NLVTGIATAYMDSVPMVAITCNVTVPLLGRDSFQEIDIAGVTMPITKHSYIVKNVKDLAKTLRNAFRIAKEGRPGPVLVD
VTKDVTAAECEYTPRVPKPVERITASIKEEDIDKAVTLIKKSKKPYVFVGGGAVISEASDELKKFVEKVDAPVCDSLMGK
GAFDGSNERYTGMLGMHGTKTSNYGVSECDLLIVVGARFSDRVTGNAKKFAKNAKILQFDVDAAEINKNVRTDAHVLGDA
KEILKRINDKLDNQDHSDWMEHIKSYKLDYPMRYNRDILNGPLIMEKIYEITNGDAIITTDVGQHQMWAAQHYKFKEPRT
LLTSGGLGTMGYGLGACIGAKVGRKDKTVVNIAGDGCFRMNMNEVATATRYNIPIIEVIFNNHVLGMVRQWQDLFYGKRY
SATVLDDQVDFVKVSEGMGAKAYSVDTIEEFEKAFKEAIELNIPCVIDCHIDREDKVFPMVSPGAAISEAFDREDLNNKK

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=576, Percent_Identity=25.6944444444444, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI21361361, Length=586, Percent_Identity=24.4027303754266, Blast_Score=158, Evalue=1e-38,
Organism=Escherichia coli, GI87081685, Length=565, Percent_Identity=40.7079646017699, Blast_Score=470, Evalue=1e-133,
Organism=Escherichia coli, GI1790104, Length=550, Percent_Identity=40, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1786717, Length=580, Percent_Identity=31.2068965517241, Blast_Score=298, Evalue=7e-82,
Organism=Escherichia coli, GI1787096, Length=549, Percent_Identity=26.4116575591985, Blast_Score=181, Evalue=9e-47,
Organism=Escherichia coli, GI1788716, Length=554, Percent_Identity=26.5342960288809, Blast_Score=177, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17531299, Length=571, Percent_Identity=28.1961471103327, Blast_Score=182, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17531301, Length=571, Percent_Identity=28.1961471103327, Blast_Score=182, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17542570, Length=572, Percent_Identity=25.1748251748252, Blast_Score=147, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6323755, Length=585, Percent_Identity=42.0512820512821, Blast_Score=443, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6320816, Length=585, Percent_Identity=23.7606837606838, Blast_Score=116, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320588, Length=538, Percent_Identity=23.0483271375465, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321524, Length=492, Percent_Identity=25.2032520325203, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323163, Length=488, Percent_Identity=25.4098360655738, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323073, Length=487, Percent_Identity=24.0246406570842, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI19922626, Length=571, Percent_Identity=25.569176882662, Blast_Score=174, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61511; Mature: 61511

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNGSEIIVECLKEQGVDTIFGYPGGAILNVYDALYKHSDEITHILTSHEQGASHAADG
CCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCC
YARATGKVGVCFATSGPGATNLVTGIATAYMDSVPMVAITCNVTVPLLGRDSFQEIDIAG
HHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCEEEECC
VTMPITKHSYIVKNVKDLAKTLRNAFRIAKEGRPGPVLVDVTKDVTAAECEYTPRVPKPV
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCHH
ERITASIKEEDIDKAVTLIKKSKKPYVFVGGGAVISEASDELKKFVEKVDAPVCDSLMGK
HHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHCC
GAFDGSNERYTGMLGMHGTKTSNYGVSECDLLIVVGARFSDRVTGNAKKFAKNAKILQFD
CCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHCCCEEEEEE
VDAAEINKNVRTDAHVLGDAKEILKRINDKLDNQDHSDWMEHIKSYKLDYPMRYNRDILN
CCHHHHCCCCCCHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
GPLIMEKIYEITNGDAIITTDVGQHQMWAAQHYKFKEPRTLLTSGGLGTMGYGLGACIGA
CCHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHC
KVGRKDKTVVNIAGDGCFRMNMNEVATATRYNIPIIEVIFNNHVLGMVRQWQDLFYGKRY
CCCCCCCEEEEEECCCEEEECHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCCC
SATVLDDQVDFVKVSEGMGAKAYSVDTIEEFEKAFKEAIELNIPCVIDCHIDREDKVFPM
EEEEECCCCCEEEECCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEE
VSPGAAISEAFDREDLNNKK
ECCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKLNGSEIIVECLKEQGVDTIFGYPGGAILNVYDALYKHSDEITHILTSHEQGASHAADG
CCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCC
YARATGKVGVCFATSGPGATNLVTGIATAYMDSVPMVAITCNVTVPLLGRDSFQEIDIAG
HHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCEEEECC
VTMPITKHSYIVKNVKDLAKTLRNAFRIAKEGRPGPVLVDVTKDVTAAECEYTPRVPKPV
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCHH
ERITASIKEEDIDKAVTLIKKSKKPYVFVGGGAVISEASDELKKFVEKVDAPVCDSLMGK
HHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHCC
GAFDGSNERYTGMLGMHGTKTSNYGVSECDLLIVVGARFSDRVTGNAKKFAKNAKILQFD
CCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHCCCEEEEEE
VDAAEINKNVRTDAHVLGDAKEILKRINDKLDNQDHSDWMEHIKSYKLDYPMRYNRDILN
CCHHHHCCCCCCHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
GPLIMEKIYEITNGDAIITTDVGQHQMWAAQHYKFKEPRTLLTSGGLGTMGYGLGACIGA
CCHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHC
KVGRKDKTVVNIAGDGCFRMNMNEVATATRYNIPIIEVIFNNHVLGMVRQWQDLFYGKRY
CCCCCCCEEEEEECCCEEEECHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCCC
SATVLDDQVDFVKVSEGMGAKAYSVDTIEEFEKAFKEAIELNIPCVIDCHIDREDKVFPM
EEEEECCCCCEEEECCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEE
VSPGAAISEAFDREDLNNKK
ECCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]