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The map label for this gene is gph [H]

Identifier: 238896853

GI number: 238896853

Start: 4817323

End: 4818084

Strand: Reverse

Name: gph [H]

Synonym: KP1_5086

Alternate gene names: 238896853

Gene position: 4818084-4817323 (Counterclockwise)

Preceding gene: 238896854

Following gene: 238896852

Centisome position: 91.8

GC content: 58.4

Gene sequence:

>762_bases
ATGGATAAATTACAGGCGATCCGCGGCATCGCATTTGATCTCGACGGCACGCTGGTGGACAGCGCGCCGGGTCTAACCTC
GGCGGTTGATAACGCCCTGTACGCCCTGGAGCTGCCGGTGGCGGGAGAAGAACGCGTTATCACCTGGATCGGTAACGGCG
CCGATGTCCTGATGGAGCGGGCGCTGACCTGGGCTCGTCAGGAGCGAGCAACGTTACGTGCGGCGATGGGCAAGCCTTCC
GTCGACGATCATGATATTCCGCAAGACGAGCAGCTGCGCATTTTGCGTAAACTGTTTGACCGCTATTACGCCGAAGCGGC
GGAAGAGGGCAGTTTCCTGTTCCCGGCGGTGGCCGATACGCTCGGCGCGCTGCATGCCAAAGGCTTGCCGCTGGCGCTGG
TTACCAATAAGCCGACGCCGTTTGTCGCCCCGCTGCTGGACGCGCTCGATATTGCGAAATATTTTACCGTGGTGATTGGC
GGCGATGACGTGCAGAATAAAAAGCCGCATCCGGAACCGCTGCTGCTGGTGGCGGAAAAGCTCTCTCTGGCTCCAGCCGA
GCTGCTGTTTGTCGGCGATTCGCGCAATGATATTCAGGCGGCCAAAGCGGCAGGCTGCTGCTCTGTTGGTCTGACCTACG
GTTATAACTATGGTGAACCGCTTGCGCTGAGCGAGCCGGACTATCTCTTCGACCAGTTTAACGAACTCTTACCCGCTCTC
GGGTTACCGCACAGTGAAACTCAGGAATTGAAACATGACTAA

Upstream 100 bases:

>100_bases
GCGGGTGCGGATATGTTTGTTGCCGGTTCGGCGATCTTCGGTCAGCCGGACTACAAGCAAGTCATTGATCAAATGCGTAG
TGAATTAGCGAAGGTGAGTC

Downstream 100 bases:

>100_bases
GCCCATCGTTTTTAGTGGCGCACAGCCCTCAGGTGAACTGACCATTGGCAACTATATGGGTGCGCTGCGTCAGTGGGTAA
ACATGCAGGACGATTACCAC

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MDKLQAIRGIAFDLDGTLVDSAPGLTSAVDNALYALELPVAGEERVITWIGNGADVLMERALTWARQERATLRAAMGKPS
VDDHDIPQDEQLRILRKLFDRYYAEAAEEGSFLFPAVADTLGALHAKGLPLALVTNKPTPFVAPLLDALDIAKYFTVVIG
GDDVQNKKPHPEPLLLVAEKLSLAPAELLFVGDSRNDIQAAKAAGCCSVGLTYGYNYGEPLALSEPDYLFDQFNELLPAL
GLPHSETQELKHD

Sequences:

>Translated_253_residues
MDKLQAIRGIAFDLDGTLVDSAPGLTSAVDNALYALELPVAGEERVITWIGNGADVLMERALTWARQERATLRAAMGKPS
VDDHDIPQDEQLRILRKLFDRYYAEAAEEGSFLFPAVADTLGALHAKGLPLALVTNKPTPFVAPLLDALDIAKYFTVVIG
GDDVQNKKPHPEPLLLVAEKLSLAPAELLFVGDSRNDIQAAKAAGCCSVGLTYGYNYGEPLALSEPDYLFDQFNELLPAL
GLPHSETQELKHD
>Mature_253_residues
MDKLQAIRGIAFDLDGTLVDSAPGLTSAVDNALYALELPVAGEERVITWIGNGADVLMERALTWARQERATLRAAMGKPS
VDDHDIPQDEQLRILRKLFDRYYAEAAEEGSFLFPAVADTLGALHAKGLPLALVTNKPTPFVAPLLDALDIAKYFTVVIG
GDDVQNKKPHPEPLLLVAEKLSLAPAELLFVGDSRNDIQAAKAAGCCSVGLTYGYNYGEPLALSEPDYLFDQFNELLPAL
GLPHSETQELKHD

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=253, Percent_Identity=81.4229249011858, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1789046, Length=203, Percent_Identity=25.615763546798, Blast_Score=60, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 27438; Mature: 27438

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLQAIRGIAFDLDGTLVDSAPGLTSAVDNALYALELPVAGEERVITWIGNGADVLMER
CCHHHHHHCEEEECCCEEECCCCCHHHHHHCCEEEEECCCCCCCEEEEEECCCHHHHHHH
ALTWARQERATLRAAMGKPSVDDHDIPQDEQLRILRKLFDRYYAEAAEEGSFLFPAVADT
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHH
LGALHAKGLPLALVTNKPTPFVAPLLDALDIAKYFTVVIGGDDVQNKKPHPEPLLLVAEK
HHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHH
LSLAPAELLFVGDSRNDIQAAKAAGCCSVGLTYGYNYGEPLALSEPDYLFDQFNELLPAL
HCCCCEEEEEECCCCCHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
GLPHSETQELKHD
CCCCCHHHHHCCC
>Mature Secondary Structure
MDKLQAIRGIAFDLDGTLVDSAPGLTSAVDNALYALELPVAGEERVITWIGNGADVLMER
CCHHHHHHCEEEECCCEEECCCCCHHHHHHCCEEEEECCCCCCCEEEEEECCCHHHHHHH
ALTWARQERATLRAAMGKPSVDDHDIPQDEQLRILRKLFDRYYAEAAEEGSFLFPAVADT
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHH
LGALHAKGLPLALVTNKPTPFVAPLLDALDIAKYFTVVIGGDDVQNKKPHPEPLLLVAEK
HHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHH
LSLAPAELLFVGDSRNDIQAAKAAGCCSVGLTYGYNYGEPLALSEPDYLFDQFNELLPAL
HCCCCEEEEEECCCCCHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
GLPHSETQELKHD
CCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11114933 [H]