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The map label for this gene is cobD

Identifier: 238896303

GI number: 238896303

Start: 4282469

End: 4283428

Strand: Reverse

Name: cobD

Synonym: KP1_4465

Alternate gene names: 238896303

Gene position: 4283428-4282469 (Counterclockwise)

Preceding gene: 238896304

Following gene: 238896302

Centisome position: 81.61

GC content: 65.0

Gene sequence:

>960_bases
ATGACCCTTCTCGCCTGGTGTGTAGCCTGGATACTTGATGTCGTCATTGGCGATCCGCCGCACTGGCCGCATCCGGTGCG
CTGGATAGGTCGCCTGATCGCCGTCAGCCAGCGTGTAGTGCGACGCATCTGCCATAGCGACCGGGCGCTGCGCATCGGCG
GCGGGGTGATGTGGCTGGTGGTTATCGGCCTGACATGGGGCGTGGCCTGGGGCGTACTGGCGCTGGCGCACGGGATCCAC
CCCTGGCTTGGATGGCTGGTGGAAGTGTGGATGATCTTCACCGCGCTGGCGGGACGCTGTCTGGCACAGTCGGCGATGGC
GGTGGCCCGGCCGCTGCAGGCCGGAGATCTCGCCGAAAGCCGGCATAAGCTCTCCTGGATTGTCGGGCGCGATACCTCTC
AGCTCCAGCCAGCGCAGATCAACCGTGCGGTGGTGGAGACCGTGGCGGAGAACACGGTGGATGGCATTATCGCGCCGCTC
TTTTTCCTGCTCCTCGGCGGCGCGCCGCTGGCCATGGCCTATAAAGCGGTCAACACCCTCGATTCCATGGTCGGCTATAA
ACACGAAAAATACCGGGCGATCGGCATGGTCAGCGCTCGTCTTGATGATGTAGCCAACTTCCTTCCCGCCCGGCTGAGCT
GGCTGCTGCTCAGCCTTGCCGCGGTGCTATGCCGGGAAGATGGCGCCCGGGCGCTGCGTACGGGCTGGCGCGATCGCTAT
CAGCACAGCAGTCCTAACTGCGCCTGGCCGGAAGCGACCGTCGCCGGCGCGCTGGGGATCCGCCTTGGCGGCCCGAACGA
TTACTTCGGCCAGCGCGTTGAAAAGCCCTGGATAGGCGACGCCGTCCGCGACATCGCGGTGGACGATATTTCCCGAACCA
TTCGATTAATGTGGGTGGCGTCAAGCCTGGCGCTGGCGCTGTTTATCGGCGTGCGCTACTGGCTGGTCGGCGCGGCCTGA

Upstream 100 bases:

>100_bases
GATGGCAGTCCGGCAGCGCCTTTGCGAGCTATCTCCATGTCCATTTTGCCCAGCGCCCGACCATGCTCAACCACTGGCTG
CAGGCAGCAAGGAGGGCGCT

Downstream 100 bases:

>100_bases
GGAGAACCTGATGCAGTACATCCAACAACCGCAGGCGATCGAGGCGAAAAGCTTCGACATCATCAGCGAGATCATCGCCG
AAACGCGACCGGACTACCGT

Product: cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTLLAWCVAWILDVVIGDPPHWPHPVRWIGRLIAVSQRVVRRICHSDRALRIGGGVMWLVVIGLTWGVAWGVLALAHGIH
PWLGWLVEVWMIFTALAGRCLAQSAMAVARPLQAGDLAESRHKLSWIVGRDTSQLQPAQINRAVVETVAENTVDGIIAPL
FFLLLGGAPLAMAYKAVNTLDSMVGYKHEKYRAIGMVSARLDDVANFLPARLSWLLLSLAAVLCREDGARALRTGWRDRY
QHSSPNCAWPEATVAGALGIRLGGPNDYFGQRVEKPWIGDAVRDIAVDDISRTIRLMWVASSLALALFIGVRYWLVGAA

Sequences:

>Translated_319_residues
MTLLAWCVAWILDVVIGDPPHWPHPVRWIGRLIAVSQRVVRRICHSDRALRIGGGVMWLVVIGLTWGVAWGVLALAHGIH
PWLGWLVEVWMIFTALAGRCLAQSAMAVARPLQAGDLAESRHKLSWIVGRDTSQLQPAQINRAVVETVAENTVDGIIAPL
FFLLLGGAPLAMAYKAVNTLDSMVGYKHEKYRAIGMVSARLDDVANFLPARLSWLLLSLAAVLCREDGARALRTGWRDRY
QHSSPNCAWPEATVAGALGIRLGGPNDYFGQRVEKPWIGDAVRDIAVDDISRTIRLMWVASSLALALFIGVRYWLVGAA
>Mature_318_residues
TLLAWCVAWILDVVIGDPPHWPHPVRWIGRLIAVSQRVVRRICHSDRALRIGGGVMWLVVIGLTWGVAWGVLALAHGIHP
WLGWLVEVWMIFTALAGRCLAQSAMAVARPLQAGDLAESRHKLSWIVGRDTSQLQPAQINRAVVETVAENTVDGIIAPLF
FLLLGGAPLAMAYKAVNTLDSMVGYKHEKYRAIGMVSARLDDVANFLPARLSWLLLSLAAVLCREDGARALRTGWRDRYQ
HSSPNCAWPEATVAGALGIRLGGPNDYFGQRVEKPWIGDAVRDIAVDDISRTIRLMWVASSLALALFIGVRYWLVGAA

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBD_KLEP7 (A6TDC6)

Other databases:

- EMBL:   CP000647
- RefSeq:   YP_001336827.1
- STRING:   A6TDC6
- GeneID:   5338941
- GenomeReviews:   CP000647_GR
- KEGG:   kpn:KPN_03199
- eggNOG:   COG1270
- HOGENOM:   HBG647131
- OMA:   RWHPLVG
- ProtClustDB:   PRK01209
- HAMAP:   MF_00024
- InterPro:   IPR004485
- TIGRFAMs:   TIGR00380

Pfam domain/function: PF03186 CobD_Cbib

EC number: 6.3.1.10

Molecular weight: Translated: 35103; Mature: 34971

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1fa050b8)-; HASH(0x19d8c120)-; HASH(0x1df37740)-; HASH(0x1f8d7714)-; HASH(0x1f77db78)-;

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLAWCVAWILDVVIGDPPHWPHPVRWIGRLIAVSQRVVRRICHSDRALRIGGGVMWLV
CHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHH
VIGLTWGVAWGVLALAHGIHPWLGWLVEVWMIFTALAGRCLAQSAMAVARPLQAGDLAES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RHKLSWIVGRDTSQLQPAQINRAVVETVAENTVDGIIAPLFFLLLGGAPLAMAYKAVNTL
HHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
DSMVGYKHEKYRAIGMVSARLDDVANFLPARLSWLLLSLAAVLCREDGARALRTGWRDRY
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
QHSSPNCAWPEATVAGALGIRLGGPNDYFGQRVEKPWIGDAVRDIAVDDISRTIRLMWVA
CCCCCCCCCCCHHHHHHHHEEECCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SSLALALFIGVRYWLVGAA
HHHHHHHHHHHHHHHHCCH
>Mature Secondary Structure 
TLLAWCVAWILDVVIGDPPHWPHPVRWIGRLIAVSQRVVRRICHSDRALRIGGGVMWLV
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHH
VIGLTWGVAWGVLALAHGIHPWLGWLVEVWMIFTALAGRCLAQSAMAVARPLQAGDLAES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RHKLSWIVGRDTSQLQPAQINRAVVETVAENTVDGIIAPLFFLLLGGAPLAMAYKAVNTL
HHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
DSMVGYKHEKYRAIGMVSARLDDVANFLPARLSWLLLSLAAVLCREDGARALRTGWRDRY
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
QHSSPNCAWPEATVAGALGIRLGGPNDYFGQRVEKPWIGDAVRDIAVDDISRTIRLMWVA
CCCCCCCCCCCHHHHHHHHEEECCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SSLALALFIGVRYWLVGAA
HHHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA