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The map label for this gene is hycB [H]

Identifier: 238896181

GI number: 238896181

Start: 4153269

End: 4153877

Strand: Reverse

Name: hycB [H]

Synonym: KP1_4328

Alternate gene names: 238896181

Gene position: 4153877-4153269 (Counterclockwise)

Preceding gene: 238896182

Following gene: 238896180

Centisome position: 79.14

GC content: 63.71

Gene sequence:

>609_bases
GTGAACCGTTTTGTAATTGCTGACTCCACTGTCTGTATCGGCTGCCGCACCTGCGAAGCCGCCTGCTCTGAGACGCATCG
CCTGCACGGCCTGCAGGCTATGCCGCGGCTGCAGGTGATGCGTAATGAAAAAGAATCGGCACCGCAGATGTGCCACCACT
GTGAAGATGCTCCCTGCGCCACCGTCTGCCCGGTTAACGCCATCCAGCGCGTCGATGGCGCGGTCCAGCTCAACGAGAGC
CTGTGCGTAAGCTGCAAACTGTGCGGCATTGCCTGCCCGTTCGGCGCCATTGAATTTTCCGGCAGCCGCCCGCTGGATAT
CCCGGCGAATGCCAATACCCGGCTGGCGCCCCCCGCGCCGCCGGCGCCGGCGCGCGTCAGCTCGCTGCTCGACTGGGTAC
CGGGCGTGCGCGCTATTGCCGTGAAATGCGACCTCTGCCACTTCGATGACCAGGGCCCGGCCTGCGTGCGGACCTGTCCG
ACCAACGCGCTGATGCTGGTGGATAGCCGTGATATCGCCCAGGCCAGCAAGCGTAAACGCCAGCTGACCTTTAATACCGA
CCTTGGCGACTTGTCGCTGTTCCAGGCGCAACAGGGGGAGAGGGAATGA

Upstream 100 bases:

>100_bases
CTGGCGCTGACCCACTGATCGTAAGGTGTCGACACGTGTCGAAAATGACACCTGCGCAACCTGATTTCGTCAAAAATGAC
TTTCACCCGAGGATGACCCG

Downstream 100 bases:

>100_bases
GTGTAATTGGATTAGTGAATCAGGCGGTGGCGTGGTATGCCGCCAGCGCCGTACTGGCCTTTCTGTTTGCCATGCGCAAA
CCGCTCAGCGGCGCCATTGC

Product: hydrogenase 3 iron-sulfur subunit

Products: Oxidized ferredoxin; CO2; H2 [C]

Alternate protein names: FHL subunit 2; Hydrogenase-3 component B [H]

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MNRFVIADSTVCIGCRTCEAACSETHRLHGLQAMPRLQVMRNEKESAPQMCHHCEDAPCATVCPVNAIQRVDGAVQLNES
LCVSCKLCGIACPFGAIEFSGSRPLDIPANANTRLAPPAPPAPARVSSLLDWVPGVRAIAVKCDLCHFDDQGPACVRTCP
TNALMLVDSRDIAQASKRKRQLTFNTDLGDLSLFQAQQGERE

Sequences:

>Translated_202_residues
MNRFVIADSTVCIGCRTCEAACSETHRLHGLQAMPRLQVMRNEKESAPQMCHHCEDAPCATVCPVNAIQRVDGAVQLNES
LCVSCKLCGIACPFGAIEFSGSRPLDIPANANTRLAPPAPPAPARVSSLLDWVPGVRAIAVKCDLCHFDDQGPACVRTCP
TNALMLVDSRDIAQASKRKRQLTFNTDLGDLSLFQAQQGERE
>Mature_202_residues
MNRFVIADSTVCIGCRTCEAACSETHRLHGLQAMPRLQVMRNEKESAPQMCHHCEDAPCATVCPVNAIQRVDGAVQLNES
LCVSCKLCGIACPFGAIEFSGSRPLDIPANANTRLAPPAPPAPARVSSLLDWVPGVRAIAVKCDLCHFDDQGPACVRTCP
TNALMLVDSRDIAQASKRKRQLTFNTDLGDLSLFQAQQGERE

Specific function: Probable electron transfer protein for hydrogenase 3 [H]

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789079, Length=201, Percent_Identity=86.5671641791045, Blast_Score=338, Evalue=2e-94,
Organism=Escherichia coli, GI87082114, Length=206, Percent_Identity=49.5145631067961, Blast_Score=191, Evalue=2e-50,
Organism=Escherichia coli, GI1789067, Length=190, Percent_Identity=40.5263157894737, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1788811, Length=188, Percent_Identity=37.7659574468085, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI2367245, Length=187, Percent_Identity=37.9679144385027, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI87082179, Length=183, Percent_Identity=35.5191256830601, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI87082180, Length=189, Percent_Identity=34.9206349206349, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI2367345, Length=204, Percent_Identity=28.4313725490196, Blast_Score=71, Evalue=4e-14,
Organism=Escherichia coli, GI1787122, Length=114, Percent_Identity=30.7017543859649, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1787872, Length=114, Percent_Identity=29.8245614035088, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 21833; Mature: 21833

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
10.4 %Cys+Met (Translated Protein)
7.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
10.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFVIADSTVCIGCRTCEAACSETHRLHGLQAMPRLQVMRNEKESAPQMCHHCEDAPCA
CCEEEEECCEEEEECCCHHHHHHHHHHHHCCHHCHHHHHHHCCCCCHHHHHHCCCCCCCC
TVCPVNAIQRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGSRPLDIPANANTRLAPPAP
EECCHHHHHHCCCCEEECCCCEEEEEECEEECCCCCEEECCCCCEECCCCCCCCCCCCCC
PAPARVSSLLDWVPGVRAIAVKCDLCHFDDQGPACVRTCPTNALMLVDSRDIAQASKRKR
CCHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCEEEEECCCCEEEEEECCHHHHHHHHHC
QLTFNTDLGDLSLFQAQQGERE
EEEEECCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MNRFVIADSTVCIGCRTCEAACSETHRLHGLQAMPRLQVMRNEKESAPQMCHHCEDAPCA
CCEEEEECCEEEEECCCHHHHHHHHHHHHCCHHCHHHHHHHCCCCCHHHHHHCCCCCCCC
TVCPVNAIQRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGSRPLDIPANANTRLAPPAP
EECCHHHHHHCCCCEEECCCCEEEEEECEEECCCCCEEECCCCCEECCCCCCCCCCCCCC
PAPARVSSLLDWVPGVRAIAVKCDLCHFDDQGPACVRTCPTNALMLVDSRDIAQASKRKR
CCHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCEEEEECCCCEEEEEECCHHHHHHHHHC
QLTFNTDLGDLSLFQAQQGERE
EEEEECCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced ferredoxin; H+; formate [C]

Specific reaction: 2 reduced ferredoxin + 2 H+ = 2 oxidized ferredoxin + H2 formate = CO2 + H2 [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]