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The map label for this gene is hycI [H]

Identifier: 238896174

GI number: 238896174

Start: 4146605

End: 4147075

Strand: Reverse

Name: hycI [H]

Synonym: KP1_4320

Alternate gene names: 238896174

Gene position: 4147075-4146605 (Counterclockwise)

Preceding gene: 238896175

Following gene: 238896170

Centisome position: 79.01

GC content: 60.3

Gene sequence:

>471_bases
GTGACTGACGTTTTACTCTGTGTCGGCAACAGCATGATGGGCGACGACGGCGCCGGCCCGCTGCTGGCGGAGATGTGCGC
GGCTAACCCGGCCGGCCAGTGGGTGGTCATCGACGGCGGCAGCGCGCCGGAAAACGACATCGTGGCGATCCGCGAATTAC
GCCCCGAACGCCTGCTGATTGTCGATGCCACCGATATGGGCCTAAACCCCGGCGACATTCGCATCATCGATCCCGACGAC
ATCGCCGAGATGTTTATGATGACCACCCATAACATGCCGCTCAACTATCTGGTCGATCAGCTGAAAGAAGATGTCGGCGA
GGTGATTTTCCTCGGCATTCAGCCGGATATCGTCGGTTTTTATTATCCGATGACCCAGCCGGTGAAAGAGGCCGTTGCCC
GGGTGTATCAGCAGCTGGCAGGCTGGCAGGGCAAGGGCGGCTTTACTCAGCTGGAGGCCGCGGACGACTAG

Upstream 100 bases:

>100_bases
GCGCAGACCCCGCAGCAGCAGGCGTGGAGCAAAACCCTGCTCAGCATGCTGCATGATATTCACCAGGAGAGCGCCATCTA
CCTGATGGTGAGGAGATTAC

Downstream 100 bases:

>100_bases
CCCTGCGGCGAACCGGGCTCTCCGCCGGCGCACGACTGGCCGGAAGGGAGCCCCGTTCCCGGCGCTGCCAGCTCAAGATT
CTCTATCACTAATTTACCGG

Product: hydrogenase 3 maturation protease

Products: NA

Alternate protein names: HycI protease [H]

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MTDVLLCVGNSMMGDDGAGPLLAEMCAANPAGQWVVIDGGSAPENDIVAIRELRPERLLIVDATDMGLNPGDIRIIDPDD
IAEMFMMTTHNMPLNYLVDQLKEDVGEVIFLGIQPDIVGFYYPMTQPVKEAVARVYQQLAGWQGKGGFTQLEAADD

Sequences:

>Translated_156_residues
MTDVLLCVGNSMMGDDGAGPLLAEMCAANPAGQWVVIDGGSAPENDIVAIRELRPERLLIVDATDMGLNPGDIRIIDPDD
IAEMFMMTTHNMPLNYLVDQLKEDVGEVIFLGIQPDIVGFYYPMTQPVKEAVARVYQQLAGWQGKGGFTQLEAADD
>Mature_155_residues
TDVLLCVGNSMMGDDGAGPLLAEMCAANPAGQWVVIDGGSAPENDIVAIRELRPERLLIVDATDMGLNPGDIRIIDPDDI
AEMFMMTTHNMPLNYLVDQLKEDVGEVIFLGIQPDIVGFYYPMTQPVKEAVARVYQQLAGWQGKGGFTQLEAADD

Specific function: Protease involved in the C-terminal processing of hycE, the large subunit of hydrogenase 3 [H]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1789072, Length=156, Percent_Identity=85.8974358974359, Blast_Score=280, Evalue=4e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004420
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: =3.4.23.51 [H]

Molecular weight: Translated: 16944; Mature: 16812

Theoretical pI: Translated: 3.75; Mature: 3.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
6.4 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVLLCVGNSMMGDDGAGPLLAEMCAANPAGQWVVIDGGSAPENDIVAIRELRPERLLI
CCCEEEEECCCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCCEEEE
VDATDMGLNPGDIRIIDPDDIAEMFMMTTHNMPLNYLVDQLKEDVGEVIFLGIQPDIVGF
EECCCCCCCCCCEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEECCCCCCEEE
YYPMTQPVKEAVARVYQQLAGWQGKGGFTQLEAADD
ECCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCC
>Mature Secondary Structure 
TDVLLCVGNSMMGDDGAGPLLAEMCAANPAGQWVVIDGGSAPENDIVAIRELRPERLLI
CCEEEEECCCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCCEEEE
VDATDMGLNPGDIRIIDPDDIAEMFMMTTHNMPLNYLVDQLKEDVGEVIFLGIQPDIVGF
EECCCCCCCCCCEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEECCCCCCEEE
YYPMTQPVKEAVARVYQQLAGWQGKGGFTQLEAADD
ECCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]