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The map label for this gene is ascG [H]

Identifier: 238896170

GI number: 238896170

Start: 4141944

End: 4142999

Strand: Reverse

Name: ascG [H]

Synonym: KP1_4316

Alternate gene names: 238896170

Gene position: 4142999-4141944 (Counterclockwise)

Preceding gene: 238896174

Following gene: 238896169

Centisome position: 78.94

GC content: 61.36

Gene sequence:

>1056_bases
GTGCAGTACACTGCCTGCAACTTTTGCAACAGGAAGAATCACATGGCAACGATGCTGGAGGTCGCAAAGCGCGCGGGGGT
ATCGAAGGCGACCGTTTCGCGGGTGCTGTCGGGCAACGGCTATGTCAGCCAGGAGACCAAAGACCGGGTGTTTAAGGCGG
TGGCGGAGAGCGGCTACCGGCCAAATCTGCTGGCGCGCAACCTGGCGACCAAAACCACGCAAACCCTCGGCCTGGTGGTG
ACCAATACCCTGTACCACGGCGTCTATTTTAGCGAACTGCTGTATCACGTGGCGCGGATGACGGAGGATAAAGGGCGGCA
GCTGATCCTGGCCGACGGCAAGCACAGCGCGCAAGAGGAGCGGGAGGCGATCCAGTACCTGCTGGATCTACGCTGCGATG
CGATCATCATCTACCCGCGCTTTCTCACCGTTGATGAGATGGACGAGATCGTTGAGAACCACTCGCAGCCGATCATGGTG
CTTAATCGCCGCCTGCGCCGCCACGCCAGCCACTGCGTCTGGTCCGATCAAAAAGCCTCGGCGATGCGGGTAGTGGAGCG
GCTGATTGCGCTTGGCCATCGCGATATCGCCTTTATTACCGGCTCCCTTGACTCTCCCACCGGCGTCGAACGCCTCGCCG
GCTACAAAGACGCGCTGGCTGGCCACGGCATCGCCCTGAACGAGGCGCTTATCGCCGAAGGGCGCTGGACGCCGGAAACC
GGGGCGGCGGGGGTGGAGAGCCTGCGTCAGCGCGGGGTTACCTACAGCGCGCTGGTGGCGAGCAATGACGATATGGCCAT
CGGGGCGATGAAGCAGCTTCACCAGCTGGGCGTTAAGGTGCCAGAACAGGTCTCGGTGGTGGGGTTTGACGATATTGCTC
TTGCGCCGTATATGGTGCCGTCGCTCGCCAGCGTCCGGGTGCCGGTGACGGAGATGATTAAAGAGAGCATCAATCGGCTG
ATCTTTATGCTGGATGGCGGGGAATTCAACTTTCAGCAAAGCTTTCCCGGAGCGCTGATTGAACGCGAGTCGCTGGTGGC
GGGCCCCTGTGCCTGA

Upstream 100 bases:

>100_bases
AATAAAAAAATATGATCTCAATTTTGTGATTTTGATCGCTAAGCGTCGCCGTGAGCCGGATCGCGGTGATGAACAGGGGA
GTGCTGTAAAAGCGGGAAAC

Downstream 100 bases:

>100_bases
CGTCGTCAGCTGTCATCGTCACTACTGACGATGACAGCAAACCGCAGCTATAATAATCTTTATAATCAATATATTAAAAA
CTTGGAACGTATTATGCATA

Product: ascBF operon repressor

Products: NA

Alternate protein names: Cryptic asc operon repressor [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MQYTACNFCNRKNHMATMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFKAVAESGYRPNLLARNLATKTTQTLGLVV
TNTLYHGVYFSELLYHVARMTEDKGRQLILADGKHSAQEEREAIQYLLDLRCDAIIIYPRFLTVDEMDEIVENHSQPIMV
LNRRLRRHASHCVWSDQKASAMRVVERLIALGHRDIAFITGSLDSPTGVERLAGYKDALAGHGIALNEALIAEGRWTPET
GAAGVESLRQRGVTYSALVASNDDMAIGAMKQLHQLGVKVPEQVSVVGFDDIALAPYMVPSLASVRVPVTEMIKESINRL
IFMLDGGEFNFQQSFPGALIERESLVAGPCA

Sequences:

>Translated_351_residues
MQYTACNFCNRKNHMATMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFKAVAESGYRPNLLARNLATKTTQTLGLVV
TNTLYHGVYFSELLYHVARMTEDKGRQLILADGKHSAQEEREAIQYLLDLRCDAIIIYPRFLTVDEMDEIVENHSQPIMV
LNRRLRRHASHCVWSDQKASAMRVVERLIALGHRDIAFITGSLDSPTGVERLAGYKDALAGHGIALNEALIAEGRWTPET
GAAGVESLRQRGVTYSALVASNDDMAIGAMKQLHQLGVKVPEQVSVVGFDDIALAPYMVPSLASVRVPVTEMIKESINRL
IFMLDGGEFNFQQSFPGALIERESLVAGPCA
>Mature_351_residues
MQYTACNFCNRKNHMATMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFKAVAESGYRPNLLARNLATKTTQTLGLVV
TNTLYHGVYFSELLYHVARMTEDKGRQLILADGKHSAQEEREAIQYLLDLRCDAIIIYPRFLTVDEMDEIVENHSQPIMV
LNRRLRRHASHCVWSDQKASAMRVVERLIALGHRDIAFITGSLDSPTGVERLAGYKDALAGHGIALNEALIAEGRWTPET
GAAGVESLRQRGVTYSALVASNDDMAIGAMKQLHQLGVKVPEQVSVVGFDDIALAPYMVPSLASVRVPVTEMIKESINRL
IFMLDGGEFNFQQSFPGALIERESLVAGPCA

Specific function: Repressor of the asc operon. The cryptic operon is activated by the insertion of IS186 into the ascG gene [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789068, Length=333, Percent_Identity=75.6756756756757, Blast_Score=540, Evalue=1e-155,
Organism=Escherichia coli, GI1788474, Length=301, Percent_Identity=35.8803986710963, Blast_Score=177, Evalue=9e-46,
Organism=Escherichia coli, GI1789202, Length=333, Percent_Identity=32.7327327327327, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1787948, Length=350, Percent_Identity=28.5714285714286, Blast_Score=151, Evalue=7e-38,
Organism=Escherichia coli, GI1790369, Length=318, Percent_Identity=31.1320754716981, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1790194, Length=308, Percent_Identity=29.5454545454545, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1786540, Length=336, Percent_Identity=29.1666666666667, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1787580, Length=320, Percent_Identity=26.5625, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI48994940, Length=318, Percent_Identity=28.6163522012579, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1790715, Length=303, Percent_Identity=25.7425742574257, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1787906, Length=284, Percent_Identity=28.5211267605634, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786268, Length=289, Percent_Identity=24.2214532871972, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790689, Length=336, Percent_Identity=23.8095238095238, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38709; Mature: 38709

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYTACNFCNRKNHMATMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFKAVAESGYR
CCCCCCCHHCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCC
PNLLARNLATKTTQTLGLVVTNTLYHGVYFSELLYHVARMTEDKGRQLILADGKHSAQEE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHH
REAIQYLLDLRCDAIIIYPRFLTVDEMDEIVENHSQPIMVLNRRLRRHASHCVWSDQKAS
HHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHH
AMRVVERLIALGHRDIAFITGSLDSPTGVERLAGYKDALAGHGIALNEALIAEGRWTPET
HHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHCHHHHHHCCCEEECHHHHCCCCCCCCC
GAAGVESLRQRGVTYSALVASNDDMAIGAMKQLHQLGVKVPEQVSVVGFDDIALAPYMVP
CHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHH
SLASVRVPVTEMIKESINRLIFMLDGGEFNFQQSFPGALIERESLVAGPCA
HHHHCCCCHHHHHHHHHHHEEEEEECCCCCCCCCCCCHHHCCCCCCCCCCC
>Mature Secondary Structure
MQYTACNFCNRKNHMATMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFKAVAESGYR
CCCCCCCHHCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCC
PNLLARNLATKTTQTLGLVVTNTLYHGVYFSELLYHVARMTEDKGRQLILADGKHSAQEE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHH
REAIQYLLDLRCDAIIIYPRFLTVDEMDEIVENHSQPIMVLNRRLRRHASHCVWSDQKAS
HHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHH
AMRVVERLIALGHRDIAFITGSLDSPTGVERLAGYKDALAGHGIALNEALIAEGRWTPET
HHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHCHHHHHHCCCEEECHHHHCCCCCCCCC
GAAGVESLRQRGVTYSALVASNDDMAIGAMKQLHQLGVKVPEQVSVVGFDDIALAPYMVP
CHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHH
SLASVRVPVTEMIKESINRLIFMLDGGEFNFQQSFPGALIERESLVAGPCA
HHHHCCCCHHHHHHHHHHHEEEEEECCCCCCCCCCCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]